BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30646.Seq (797 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac... 177 2e-45 SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|S... 43 6e-05 SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schi... 43 6e-05 SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schiz... 42 1e-04 SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schiz... 40 3e-04 SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizo... 36 0.007 SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 34 0.027 SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomy... 34 0.027 SPAC17A5.01 |pex6||peroxin-6 |Schizosaccharomyces pombe|chr 1|||... 32 0.11 SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizo... 31 0.14 SPCC24B10.10c |||mitochondrial outer membrane ATPase Msp1 |Schiz... 31 0.25 SPAC1556.02c |sdh1||succinate dehydrogenase Sdh1|Schizosaccharom... 30 0.33 SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit ... 29 0.58 SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schi... 29 0.77 SPBC16E9.10c |||AAA family ATPase Rix7 |Schizosaccharomyces pomb... 29 0.77 SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein S15a... 29 1.0 SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces po... 29 1.0 SPAC328.04 |||AAA family ATPase, unknown biological role|Schizos... 29 1.0 SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein S... 29 1.0 SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac... 28 1.8 SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr... 27 2.4 SPBC11C11.10 ||SPBC3B8.13c|pseudouridine synthase|Schizosaccharo... 27 3.1 SPBP4G3.03 |||PI31 proteasome regulator related|Schizosaccharomy... 26 5.4 SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.2 SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr ... 26 7.2 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.2 SPCC11E10.05c |ynd1||nucleoside diphosphatase |Schizosaccharomyc... 26 7.2 SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun... 25 9.5 SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyce... 25 9.5 SPBC947.01 |||AAA family ATPase, unknown biological role|Schizos... 25 9.5 >SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosaccharomyces pombe|chr 1|||Manual Length = 815 Score = 177 bits (431), Expect = 2e-45 Identities = 83/174 (47%), Positives = 112/174 (64%) Frame = +1 Query: 223 LAQGQTPQGNRCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 402 + +G+ + IVL+D+ D R+ D+V+I PCP +KY +R+ +L Sbjct: 78 VVKGKRRKDTVLIVLTDEEMEDGVARINRVVRNNLRVRLGDIVTINPCPDIKYAERISVL 137 Query: 403 PIDDSVEGLTGNLFEVYLKPYFMEAYRPIHRDDTFRSAGACAPSSSKWSKQIHHHFASWA 582 P+ D+VEGLTG+LF+VYLKPYF+EAYRPI + D F G+ K F + Sbjct: 138 PLADTVEGLTGSLFDVYLKPYFVEAYRPIRKGDLFVVRGSMRQVEFKVVDVAPDEFGIVS 197 Query: 583 PDTVIHCDGEPIKRXEEEEALXAVGYDDIGGCRKQLAQIKEMVELPLRHPSLFK 744 DT+IH +GEPI R +EE +L VGYDDIGGCR+Q+AQI+E+VELPLRHP LFK Sbjct: 198 QDTIIHWEGEPINREDEESSLAEVGYDDIGGCRRQMAQIRELVELPLRHPQLFK 251 Score = 88.6 bits (210), Expect = 9e-19 Identities = 41/68 (60%), Positives = 53/68 (77%) Frame = +2 Query: 53 NKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLK 232 N D S +D +TAILR+K +PN L+V++A +DDNSV+ LS ME LQLFRGDTV++K Sbjct: 21 NPPKDTYSAEDTATAILRKKRKPNSLVVDDATNDDNSVITLSSNTMETLQLFRGDTVVVK 80 Query: 233 GKRRKETV 256 GKRRK+TV Sbjct: 81 GKRRKDTV 88 >SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|Schizosaccharomyces pombe|chr 2|||Manual Length = 403 Score = 42.7 bits (96), Expect = 6e-05 Identities = 15/29 (51%), Positives = 24/29 (82%) Frame = +1 Query: 658 YDDIGGCRKQLAQIKEMVELPLRHPSLFK 744 Y+ +GG KQ+ +IKE++ELP++HP LF+ Sbjct: 144 YEMVGGLEKQIKEIKEVIELPVKHPELFE 172 >SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 42.7 bits (96), Expect = 6e-05 Identities = 19/47 (40%), Positives = 27/47 (57%) Frame = +1 Query: 604 DGEPIKRXEEEEALXAVGYDDIGGCRKQLAQIKEMVELPLRHPSLFK 744 D +P+ + + Y DIGG Q+ +IKE VELPL HP L++ Sbjct: 172 DTDPMINVMKLDKAPTESYADIGGLESQIQEIKEAVELPLTHPELYE 218 >SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 809 Score = 41.5 bits (93), Expect = 1e-04 Identities = 17/32 (53%), Positives = 25/32 (78%) Frame = +1 Query: 649 AVGYDDIGGCRKQLAQIKEMVELPLRHPSLFK 744 AV + IGG + Q+AQI+++VELP ++P LFK Sbjct: 275 AVTFSSIGGLQAQIAQIRDIVELPFQNPELFK 306 Score = 27.9 bits (59), Expect = 1.8 Identities = 13/30 (43%), Positives = 17/30 (56%) Frame = +1 Query: 652 VGYDDIGGCRKQLAQIKEMVELPLRHPSLF 741 V + DIGG + ++KE VE PL H F Sbjct: 545 VHWSDIGGQEEVKQKLKESVEWPLTHGETF 574 >SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 40.3 bits (90), Expect = 3e-04 Identities = 15/30 (50%), Positives = 23/30 (76%) Frame = +1 Query: 652 VGYDDIGGCRKQLAQIKEMVELPLRHPSLF 741 V Y D+GGC++Q+ +++E+VELPL P F Sbjct: 176 VTYGDVGGCKEQIERLREVVELPLLSPERF 205 >SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizosaccharomyces pombe|chr 3|||Manual Length = 388 Score = 35.9 bits (79), Expect = 0.007 Identities = 11/30 (36%), Positives = 23/30 (76%) Frame = +1 Query: 652 VGYDDIGGCRKQLAQIKEMVELPLRHPSLF 741 + + +GG +Q+ +++E++ELPL++P LF Sbjct: 129 ISFAGVGGLNEQIRELREVIELPLKNPELF 158 >SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1190 Score = 33.9 bits (74), Expect = 0.027 Identities = 12/34 (35%), Positives = 23/34 (67%) Frame = +1 Query: 649 AVGYDDIGGCRKQLAQIKEMVELPLRHPSLFKGY 750 ++ ++ +GG + Q+KEMV LPL +P +F+ + Sbjct: 262 SLSFESVGGLDNYINQLKEMVMLPLLYPEIFQRF 295 >SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 1201 Score = 33.9 bits (74), Expect = 0.027 Identities = 16/48 (33%), Positives = 26/48 (54%) Frame = +1 Query: 598 HCDGEPIKRXEEEEALXAVGYDDIGGCRKQLAQIKEMVELPLRHPSLF 741 H D + I+ + + ++ IGG + Q+KEMV LPL +P +F Sbjct: 351 HIDEKTIRSTDPFANRENLDFNSIGGLEDIILQLKEMVMLPLLYPEVF 398 >SPAC17A5.01 |pex6||peroxin-6 |Schizosaccharomyces pombe|chr 1|||Manual Length = 948 Score = 31.9 bits (69), Expect = 0.11 Identities = 12/30 (40%), Positives = 20/30 (66%) Frame = +1 Query: 652 VGYDDIGGCRKQLAQIKEMVELPLRHPSLF 741 V +DDIGG + +++ ++LPL+ P LF Sbjct: 652 VNWDDIGGLEEAKTVLRDTLQLPLQFPELF 681 >SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizosaccharomyces pombe|chr 3|||Manual Length = 389 Score = 31.5 bits (68), Expect = 0.14 Identities = 13/31 (41%), Positives = 19/31 (61%) Frame = +1 Query: 652 VGYDDIGGCRKQLAQIKEMVELPLRHPSLFK 744 V Y D+GG Q +++E VELPL L++ Sbjct: 131 VSYADVGGLDVQKQEVREAVELPLTQGDLYR 161 >SPCC24B10.10c |||mitochondrial outer membrane ATPase Msp1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 355 Score = 30.7 bits (66), Expect = 0.25 Identities = 12/30 (40%), Positives = 20/30 (66%) Frame = +1 Query: 652 VGYDDIGGCRKQLAQIKEMVELPLRHPSLF 741 V +DDIGG + + Q+ + V PL++P +F Sbjct: 86 VSFDDIGGMDEHVNQLLQDVLFPLKYPEVF 115 >SPAC1556.02c |sdh1||succinate dehydrogenase Sdh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 641 Score = 30.3 bits (65), Expect = 0.33 Identities = 19/56 (33%), Positives = 28/56 (50%), Gaps = 1/56 (1%) Frame = -1 Query: 548 DHFELDGAHAPAD-LKVSSRWIGR*ASMKYGFKYTSNRLPVRPSTESSIGSMWTRF 384 DH L +H PA+ LK I A++ G T +PV P+ ++G + TRF Sbjct: 355 DHCYLQLSHLPAEILKERLPGISETAAIFAGVDVTKEPIPVLPTVHYNMGGIPTRF 410 >SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit Rpt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 438 Score = 29.5 bits (63), Expect = 0.58 Identities = 11/29 (37%), Positives = 18/29 (62%) Frame = +1 Query: 658 YDDIGGCRKQLAQIKEMVELPLRHPSLFK 744 Y DIGG KQ+ ++ E + LP++ F+ Sbjct: 184 YSDIGGLSKQIEELFEAIVLPMQQADKFR 212 >SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schizosaccharomyces pombe|chr 1|||Manual Length = 319 Score = 29.1 bits (62), Expect = 0.77 Identities = 12/39 (30%), Positives = 20/39 (51%), Gaps = 1/39 (2%) Frame = +3 Query: 78 LMIYRPXSSVARTDPTVSLSKKQSAMTTQSWH-FHRPKW 191 L + R + DP ++ + Q + T+SWH FH +W Sbjct: 259 LALIRGCDGLLSDDPVMARALSQGPIVTKSWHYFHYSEW 297 >SPBC16E9.10c |||AAA family ATPase Rix7 |Schizosaccharomyces pombe|chr 2|||Manual Length = 779 Score = 29.1 bits (62), Expect = 0.77 Identities = 9/31 (29%), Positives = 20/31 (64%) Frame = +1 Query: 652 VGYDDIGGCRKQLAQIKEMVELPLRHPSLFK 744 + DIGG + ++ E+V +P++HP +++ Sbjct: 171 ISLSDIGGLDDCINELLELVAMPIKHPEVYQ 201 >SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein S15a|Schizosaccharomyces pombe|chr 1|||Manual Length = 130 Score = 28.7 bits (61), Expect = 1.0 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%) Frame = -2 Query: 421 QLNHQLAVCGLV---FHISLKDTERWI 350 QLN ++ CG++ F++ LKD E+W+ Sbjct: 64 QLNGRINKCGVISPRFNVKLKDIEKWV 90 >SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces pombe|chr 1|||Manual Length = 432 Score = 28.7 bits (61), Expect = 1.0 Identities = 13/30 (43%), Positives = 17/30 (56%) Frame = +1 Query: 652 VGYDDIGGCRKQLAQIKEMVELPLRHPSLF 741 V +DDI G +KE V LP++ P LF Sbjct: 126 VRWDDIAGLENAKEALKETVLLPIKLPQLF 155 >SPAC328.04 |||AAA family ATPase, unknown biological role|Schizosaccharomyces pombe|chr 1|||Manual Length = 741 Score = 28.7 bits (61), Expect = 1.0 Identities = 14/35 (40%), Positives = 17/35 (48%) Frame = +1 Query: 652 VGYDDIGGCRKQLAQIKEMVELPLRHPSLFKGYXE 756 V +DDI G +KE V P P LF+G E Sbjct: 456 VHWDDISGLEFAKHSLKEAVVYPFLRPDLFQGLRE 490 >SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein S15a|Schizosaccharomyces pombe|chr 1|||Manual Length = 130 Score = 28.7 bits (61), Expect = 1.0 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%) Frame = -2 Query: 421 QLNHQLAVCGLV---FHISLKDTERWI 350 QLN ++ CG++ F++ LKD E+W+ Sbjct: 64 QLNGRINKCGVISPRFNVKLKDIEKWV 90 >SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 1|||Manual Length = 905 Score = 27.9 bits (59), Expect = 1.8 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%) Frame = -2 Query: 391 LVFHISLKDTERWIP-HQKDAHEGCFSQHGSSEFSHQG 281 LV + LKD WI + + E F G ++FS+QG Sbjct: 435 LVSDLYLKDLWSWIHLSHRQSEESLFGDTGDTDFSYQG 472 >SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 382 Score = 27.5 bits (58), Expect = 2.4 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 2/62 (3%) Frame = +3 Query: 27 FLDSNNKF*IKWQIIKALMIYRPXSSVARTDPTVS--LSKKQSAMTTQSWHFHRPKWSNF 200 F+++N K KW++ A+ P S + + PT+S + + SA TT +RP S Sbjct: 286 FIEANIK---KWRLAPAVGSLAPPPSYSTSQPTISWDAASESSAGTTTQGDTNRPDKSKN 342 Query: 201 NS 206 S Sbjct: 343 RS 344 >SPBC11C11.10 ||SPBC3B8.13c|pseudouridine synthase|Schizosaccharomyces pombe|chr 2|||Manual Length = 407 Score = 27.1 bits (57), Expect = 3.1 Identities = 22/61 (36%), Positives = 26/61 (42%), Gaps = 1/61 (1%) Frame = +2 Query: 503 PSGPRGHARRRVQSGRNRSITILHRGLLIP*YT-VTVNRLNVXKKRKHXMLSAMMTSAVV 679 P P RRR +S R I I H G L P + V V L K+ +LS M T Sbjct: 87 PPHPNDRNRRRRKSNRLPDIKIGHGGTLDPLASGVLVVGLGTGTKQLSSLLSCMKTYRAT 146 Query: 680 A 682 A Sbjct: 147 A 147 >SPBP4G3.03 |||PI31 proteasome regulator related|Schizosaccharomyces pombe|chr 2|||Manual Length = 241 Score = 26.2 bits (55), Expect = 5.4 Identities = 14/48 (29%), Positives = 21/48 (43%) Frame = -2 Query: 391 LVFHISLKDTERWIPHQKDAHEGCFSQHGSSEFSHQGNYRLRARCNGF 248 L FH ++ D W PH+ + G F H + +G + R GF Sbjct: 177 LGFHPNVGDYFDWYPHRPNHFGGKFDGHSRGRGAGRGGFCGRHGRGGF 224 >SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 452 Score = 25.8 bits (54), Expect = 7.2 Identities = 14/47 (29%), Positives = 25/47 (53%) Frame = +3 Query: 99 SSVARTDPTVSLSKKQSAMTTQSWHFHRPKWSNFNSSVVTQSCSRAN 239 SSV S S ++M++ + +F+ S+ +SSV T + S +N Sbjct: 329 SSVVIVQSETSCSINSASMSSNTTYFYWNSTSSLSSSVFTNTTSSSN 375 >SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 409 Score = 25.8 bits (54), Expect = 7.2 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 1/27 (3%) Frame = +2 Query: 77 PD-DLSTAILRRKDRPNRLIVEEAVSD 154 PD D + + + RK+ PNRL++ A+S+ Sbjct: 233 PDADANISKIARKEDPNRLVLASAISE 259 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 7.2 Identities = 14/53 (26%), Positives = 30/53 (56%) Frame = +2 Query: 50 LNKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLF 208 L +A N + L+T +RK + N ++ +A ++ N+ +++ A E+ QL+ Sbjct: 768 LGSLAGNLPQESLTTKS-KRKSKVNLELMFDATAEKNNENSMNSAVFEKSQLY 819 >SPCC11E10.05c |ynd1||nucleoside diphosphatase |Schizosaccharomyces pombe|chr 3|||Manual Length = 572 Score = 25.8 bits (54), Expect = 7.2 Identities = 19/48 (39%), Positives = 24/48 (50%) Frame = +1 Query: 631 EEEALXAVGYDDIGGCRKQLAQIKEMVELPLRHPSLFKGYXE*SSTRH 774 EE+ VG+ D+GG Q+A ELP PS K Y + ST H Sbjct: 161 EEKDTSTVGFLDMGGASVQIA-----FELP---PSQLKNYKDSISTVH 200 >SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 214 Score = 25.4 bits (53), Expect = 9.5 Identities = 22/84 (26%), Positives = 35/84 (41%), Gaps = 8/84 (9%) Frame = +1 Query: 439 LFEVYLKPYFMEAYRPIHRDD-TFRSAG-----ACAPSSSKWSKQIHHHFASWAPDTV-- 594 L + L F+E Y P D F++ G A P+ ++W KQI + + P T Sbjct: 15 LNDFLLDKSFIEGYEPSQADAVVFKAVGVAPDTAKYPNGARWYKQIATYDLATLPGTAKE 74 Query: 595 IHCDGEPIKRXEEEEALXAVGYDD 666 + G EE+ + G D+ Sbjct: 75 VSAYGPEGAAAAEEDEIDLFGSDE 98 >SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyces pombe|chr 3|||Manual Length = 1008 Score = 25.4 bits (53), Expect = 9.5 Identities = 10/38 (26%), Positives = 22/38 (57%) Frame = +2 Query: 137 EEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKRRKE 250 E+AV D+N+ ++L+ + + RGD ++ G ++ Sbjct: 247 EKAVCDENTKISLTNTEHYKFHSLRGDVEVVVGDLERD 284 >SPBC947.01 |||AAA family ATPase, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 660 Score = 25.4 bits (53), Expect = 9.5 Identities = 13/35 (37%), Positives = 16/35 (45%) Frame = +1 Query: 652 VGYDDIGGCRKQLAQIKEMVELPLRHPSLFKGYXE 756 V + DI G +KE V P P LF+G E Sbjct: 376 VYWSDIAGLDDAKNSLKEAVIYPFLRPELFQGLRE 410 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,197,849 Number of Sequences: 5004 Number of extensions: 62366 Number of successful extensions: 206 Number of sequences better than 10.0: 30 Number of HSP's better than 10.0 without gapping: 192 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 205 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 389395636 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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