BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30645.Seq (551 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_37381| Best HMM Match : Ribosomal_S12 (HMM E-Value=0) 126 9e-30 SB_33031| Best HMM Match : Ribosomal_S12 (HMM E-Value=0) 85 5e-17 SB_34124| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.5 SB_52986| Best HMM Match : zf-C2H2 (HMM E-Value=0.0042) 29 3.3 SB_48401| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.3 SB_46453| Best HMM Match : DUF947 (HMM E-Value=0.13) 28 4.4 SB_32242| Best HMM Match : Pam16 (HMM E-Value=7.4e-20) 27 7.7 SB_54786| Best HMM Match : Ribosomal_S12 (HMM E-Value=1.4e-12) 27 7.7 >SB_37381| Best HMM Match : Ribosomal_S12 (HMM E-Value=0) Length = 413 Score = 126 bits (305), Expect = 9e-30 Identities = 57/66 (86%), Positives = 62/66 (93%) Frame = +3 Query: 51 NHRREQRWADKEFKKAHMGTKWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQL 230 +HRR+Q+W DK +KKAH+GT KANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQL Sbjct: 14 SHRRDQKWHDKAYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQL 73 Query: 231 IKNGKK 248 IKNGKK Sbjct: 74 IKNGKK 79 Score = 37.5 bits (83), Expect = 0.007 Identities = 15/21 (71%), Positives = 17/21 (80%) Frame = +2 Query: 242 KESDPFVPRDGCLNHIEENDE 304 K+ FVP DGCLN+IEENDE Sbjct: 78 KKITAFVPNDGCLNYIEENDE 98 Score = 37.5 bits (83), Expect = 0.007 Identities = 13/17 (76%), Positives = 17/17 (100%) Frame = +1 Query: 310 VAGFGRKGHAVGDIPGV 360 ++GFGR+GHAVGDIPG+ Sbjct: 101 ISGFGRRGHAVGDIPGI 117 >SB_33031| Best HMM Match : Ribosomal_S12 (HMM E-Value=0) Length = 143 Score = 84.6 bits (200), Expect = 5e-17 Identities = 38/42 (90%), Positives = 42/42 (100%) Frame = +1 Query: 310 VAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKEKKERPRS 435 ++GFGR+GHAVGDIPGVRFKVVKVANVSLLAL+KEKKERPRS Sbjct: 102 ISGFGRRGHAVGDIPGVRFKVVKVANVSLLALFKEKKERPRS 143 Score = 55.2 bits (127), Expect = 3e-08 Identities = 25/28 (89%), Positives = 27/28 (96%) Frame = +3 Query: 165 EKVGVEAKQPNSAIRKCVRVQLIKNGKK 248 ++ GVEAKQPNSAIRKCVRVQLIKNGKK Sbjct: 53 QEPGVEAKQPNSAIRKCVRVQLIKNGKK 80 Score = 37.5 bits (83), Expect = 0.007 Identities = 15/21 (71%), Positives = 17/21 (80%) Frame = +2 Query: 242 KESDPFVPRDGCLNHIEENDE 304 K+ FVP DGCLN+IEENDE Sbjct: 79 KKITAFVPNDGCLNYIEENDE 99 >SB_34124| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 468 Score = 29.1 bits (62), Expect = 2.5 Identities = 15/33 (45%), Positives = 18/33 (54%) Frame = +2 Query: 95 SPHGYEMEG*PFRWCISRKGHRPRESWCRS*AA 193 S HG MEG P W +S G P+ S C S +A Sbjct: 85 SQHGTRMEGVPVCWYVSVWGLSPQVSQCDSVSA 117 >SB_52986| Best HMM Match : zf-C2H2 (HMM E-Value=0.0042) Length = 623 Score = 28.7 bits (61), Expect = 3.3 Identities = 17/43 (39%), Positives = 22/43 (51%) Frame = +2 Query: 401 LSTKRKRSDQDHRCTL**VTCCREARCL*VHKM*NPVHNKYCV 529 +S+KR RS+ + C V CC E V K +PV K CV Sbjct: 1 MSSKRTRSESSNLC----VVCCEEIEFSAVGKCDHPVCYKCCV 39 >SB_48401| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 694 Score = 28.7 bits (61), Expect = 3.3 Identities = 17/43 (39%), Positives = 22/43 (51%) Frame = +2 Query: 401 LSTKRKRSDQDHRCTL**VTCCREARCL*VHKM*NPVHNKYCV 529 +S+KR RS+ + C V CC E V K +PV K CV Sbjct: 1 MSSKRTRSESSNLC----VVCCEEIEFSAVGKCDHPVCYKCCV 39 >SB_46453| Best HMM Match : DUF947 (HMM E-Value=0.13) Length = 943 Score = 28.3 bits (60), Expect = 4.4 Identities = 17/35 (48%), Positives = 22/35 (62%), Gaps = 3/35 (8%) Frame = -2 Query: 163 RTMPFA*D-APPKGLAFHFVPMW-AFLN-SLSAHR 68 R +PF+ APPKG F VP+W +F N S+ HR Sbjct: 902 RKVPFSSVLAPPKGYRFLIVPLWRSFSNRSVFGHR 936 >SB_32242| Best HMM Match : Pam16 (HMM E-Value=7.4e-20) Length = 255 Score = 27.5 bits (58), Expect = 7.7 Identities = 12/31 (38%), Positives = 20/31 (64%) Frame = +1 Query: 55 TVVNSDGRTKNSRKPTWVRNGRLTLSVVHLT 147 T V R N++KP +R+GR+T+S+ +T Sbjct: 103 THVQFHHRDTNTKKPVHLRDGRVTVSLAAMT 133 >SB_54786| Best HMM Match : Ribosomal_S12 (HMM E-Value=1.4e-12) Length = 302 Score = 27.5 bits (58), Expect = 7.7 Identities = 14/32 (43%), Positives = 21/32 (65%) Frame = +3 Query: 150 KGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGK 245 KG+ ++ + K+PNSA RKC ++L NGK Sbjct: 230 KGVCVKVFIRKPKKPNSAQRKCALLKL-SNGK 260 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,131,228 Number of Sequences: 59808 Number of extensions: 403582 Number of successful extensions: 753 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 676 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 753 length of database: 16,821,457 effective HSP length: 78 effective length of database: 12,156,433 effective search space used: 1276425465 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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