Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30643.Seq
         (748 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit...    33   0.043
SPAC20G4.05c |||UPF0061 family protein|Schizosaccharomyces pombe...    27   2.8  
SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch...    27   2.8  
SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po...    27   2.8  
SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces...    27   3.8  
SPBC28F2.09 |||transcription factor TFIIA complex large subunit ...    26   5.0  
SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2...    26   5.0  
SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|c...    26   6.6  
SPBC119.10 |asn1||asparagine synthetase|Schizosaccharomyces pomb...    26   6.6  

>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 409

 Score = 33.1 bits (72), Expect = 0.043
 Identities = 29/138 (21%), Positives = 61/138 (44%), Gaps = 3/138 (2%)
 Frame = +3

Query: 252 SRVWQWASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFL 431
           ++V Q+ S+++SEL  A    + P +    +++Q  + A++ +       D  L ++T+L
Sbjct: 91  AKVLQYCSFTNSELPGAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDEELASKTYL 150

Query: 432 VTERITCR---CHCLQYTAACFPARARPDVRSSLINVQRWFLTVAHQPQVSAVVGSLTLC 602
           V  R+T       C     A +         +   ++ R++ T+ HQ ++ A+   L   
Sbjct: 151 VGSRLTLADIFFTCFLKFGATYV--LTKSYLAKYTHIYRYYQTIYHQAKLDAITEPLKFI 208

Query: 603 AAPPTYDPKKYQELAGAQ 656
             P      + +E A A+
Sbjct: 209 DQPLPIIKAENKEAAPAK 226



 Score = 31.9 bits (69), Expect = 0.100
 Identities = 17/39 (43%), Positives = 23/39 (58%)
 Frame = +1

Query: 103 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN 219
           N   D   KFP  K+P F   DG   L+E+ AIA+Y+A+
Sbjct: 38  NFPADLAAKFPLQKMPVFVGKDG-FPLSETLAIAFYLAS 75


>SPAC20G4.05c |||UPF0061 family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 568

 Score = 27.1 bits (57), Expect = 2.8
 Identities = 24/65 (36%), Positives = 32/65 (49%), Gaps = 1/65 (1%)
 Frame = +1

Query: 97  ETNKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVANESLRG-GDLATQAVSGSGH 273
           ET K  +   K  AG+ P    ADGK +L  S  I  Y+  E+L   G   TQA++ S  
Sbjct: 134 ETGKRFEIQVK-GAGRTPYSRFADGKAVLRSS--IREYLCCEALYALGIPTTQALAISNL 190

Query: 274 HGLTA 288
            G+ A
Sbjct: 191 EGVVA 195


>SPAPB1E7.06c |eme1||Holliday junction resolvase subunit
           Eme1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 738

 Score = 27.1 bits (57), Expect = 2.8
 Identities = 16/62 (25%), Positives = 30/62 (48%)
 Frame = +1

Query: 10  PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 189
           P  F++ KA +  QY+          +FG  +K ++F K+    ++  F S +  +L+  
Sbjct: 553 PNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEKLESEIVRF-SFEHSILINT 611

Query: 190 SN 195
           SN
Sbjct: 612 SN 613


>SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 220

 Score = 27.1 bits (57), Expect = 2.8
 Identities = 16/34 (47%), Positives = 18/34 (52%)
 Frame = +1

Query: 109 SEDFLKKFPAGKVPAFESADGKVLLTESNAIAYY 210
           S D   KFP  K+P F  ADG   L+E  AI  Y
Sbjct: 40  SADLAAKFPLQKLPVFIGADG-FELSEVIAIVKY 72



 Score = 27.1 bits (57), Expect = 2.8
 Identities = 18/88 (20%), Positives = 42/88 (47%), Gaps = 1/88 (1%)
 Frame = +3

Query: 312 WVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERIT-CRCHCLQYTAACF 488
           WV  + G + + ++  + + +  + +LK+ +  +  RT+LV +R T             F
Sbjct: 116 WVGMFRGNIPYEEKPFKESATRAIDSLKIPNELVKDRTYLVGDRFTLADLFFGSLLRIFF 175

Query: 489 PARARPDVRSSLINVQRWFLTVAHQPQV 572
            +      R  L ++ R+++T+ HQ ++
Sbjct: 176 NSIIDEKTRKELPHLTRYYITMFHQAKL 203


>SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 442

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 11/27 (40%), Positives = 15/27 (55%)
 Frame = +1

Query: 376 TYWPP*KYWTDIFSHAPSLLPRESLAD 456
           TY PP  Y  D+FS APS+   +   +
Sbjct: 257 TYLPPFNYDHDVFSFAPSVASADQFTE 283


>SPBC28F2.09 |||transcription factor TFIIA complex large subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 369

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 12/25 (48%), Positives = 14/25 (56%)
 Frame = -3

Query: 179 STFPSALSNAGTFPAGNFFKKSSDL 105
           +TFP A +  GTFP G  F   S L
Sbjct: 52  ATFPWAQAPVGTFPIGQLFDPVSGL 76


>SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis
           Mei2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 750

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 13/33 (39%), Positives = 19/33 (57%)
 Frame = +1

Query: 406 DIFSHAPSLLPRESLADVIVFSTLLHAFQHVLD 504
           DIFSHA   L   +L  ++ ++TLL  F  + D
Sbjct: 188 DIFSHASRYLFVTNLPRIVPYATLLELFSKLGD 220


>SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 339

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 3/39 (7%)
 Frame = +1

Query: 268 GHHGLTANYCLLPALGSSLTLV--SCNSTN-RMLNVQSL 375
           GH  L  + C  P++GSS  L+  +C   N R+  V++L
Sbjct: 213 GHTDLIRDICWAPSMGSSYYLIATACKDGNVRIFKVETL 251


>SPBC119.10 |asn1||asparagine synthetase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 557

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 17/66 (25%), Positives = 29/66 (43%)
 Frame = +1

Query: 298 LLPALGSSLTLVSCNSTNRMLNVQSLTYWPP*KYWTDIFSHAPSLLPRESLADVIVFSTL 477
           L+ ++ +  T    NST++    +++T WP    +      +P LL    +AD       
Sbjct: 244 LIASIAARETEKLANSTSQSEEARTITAWPKLHSFAIGLPGSPDLLAARKVAD------F 297

Query: 478 LHAFQH 495
           LH F H
Sbjct: 298 LHTFHH 303


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,088,628
Number of Sequences: 5004
Number of extensions: 64861
Number of successful extensions: 210
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 210
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 355273338
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)