Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30643.Seq
(748 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 71 6e-13
At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 70 2e-12
At1g02930.1 68414.m00260 glutathione S-transferase, putative sim... 44 8e-05
At4g02520.1 68417.m00345 glutathione S-transferase, putative 43 2e-04
At2g02930.1 68415.m00241 glutathione S-transferase, putative 42 3e-04
At1g02920.1 68414.m00259 glutathione S-transferase, putative sim... 40 0.001
At1g02950.2 68414.m00263 glutathione S-transferase, putative sim... 38 0.005
At1g02950.1 68414.m00262 glutathione S-transferase, putative sim... 38 0.005
At2g02380.1 68415.m00176 glutathione S-transferase, putative sim... 38 0.007
At1g02940.1 68414.m00261 glutathione S-transferase, putative sim... 38 0.009
At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1... 36 0.022
At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1... 36 0.022
At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1... 33 0.20
At2g28290.2 68415.m03434 chromatin remodeling protein, putative ... 30 1.9
At2g28290.1 68415.m03433 chromatin remodeling protein, putative ... 30 1.9
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 30 1.9
At5g46550.1 68418.m05731 DNA-binding bromodomain-containing prot... 29 2.5
At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N domain-... 29 2.5
At1g16900.1 68414.m02047 curculin-like (mannose-binding) lectin ... 29 2.5
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 29 3.3
At4g22130.1 68417.m03199 protein kinase family protein contains ... 29 4.3
At1g27850.1 68414.m03413 expressed protein similar to En/Spm-lik... 28 7.6
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 71.3 bits (167), Expect = 6e-13
Identities = 41/89 (46%), Positives = 54/89 (60%), Gaps = 4/89 (4%)
Frame = +1
Query: 1 YTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVL 180
+TY N A KALIAA+Y+G ++ + +F G TNKS +FLK P GKVP E+ +G +
Sbjct: 6 HTYKGNKGANKALIAAEYAGVKIEESADFQMGVTNKSPEFLKMNPIGKVPVLETPEGPIF 65
Query: 181 LTESNAIAYYVA----NESLRGGDLATQA 255
ESNAIA YV+ + SL G L A
Sbjct: 66 --ESNAIARYVSRKNGDNSLNGSSLIEYA 92
Score = 50.0 bits (114), Expect = 2e-06
Identities = 35/128 (27%), Positives = 54/128 (42%), Gaps = 1/128 (0%)
Frame = +3
Query: 252 SRVWQWASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFL 431
+ + QW +S E+ W P +G F+ E A S L L+ L+ HL + TFL
Sbjct: 92 AHIEQWIDFSSLEIDANMLKWFAPRMGYAPFSAPAEEAAISALKRGLEALNTHLASNTFL 151
Query: 432 VTERITCR-CHCLQYTAACFPARARPDVRSSLINVQRWFLTVAHQPQVSAVVGSLTLCAA 608
V +T + F S+ +V+R+F T+ +QP+ V+G A
Sbjct: 152 VGHSVTLADIVTICNLNLGFATVMTKKFTSAFPHVERYFWTMVNQPEFKKVLGDAKQTEA 211
Query: 609 PPTYDPKK 632
P KK
Sbjct: 212 VPPVPTKK 219
>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative Similar to elongation factor
1-gamma (gb|EF1G_XENLA). ESTs
gb|T20564,gb|T45940,gb|T04527 come from this gene
Length = 414
Score = 69.7 bits (163), Expect = 2e-12
Identities = 42/89 (47%), Positives = 51/89 (57%), Gaps = 4/89 (4%)
Frame = +1
Query: 1 YTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVL 180
+TY N A KALIAA+Y G + V +F G TNK+ FLK P GKVP E+ +G V
Sbjct: 6 HTYKGNKSAEKALIAAEYVGVQIDVPSDFQMGVTNKTPAFLKMNPIGKVPVLETPEGSVF 65
Query: 181 LTESNAIAYYVA----NESLRGGDLATQA 255
ESNAIA YV+ + SL G L A
Sbjct: 66 --ESNAIARYVSRLNGDNSLNGSSLIEYA 92
Score = 54.0 bits (124), Expect = 1e-07
Identities = 37/128 (28%), Positives = 56/128 (43%), Gaps = 1/128 (0%)
Frame = +3
Query: 252 SRVWQWASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFL 431
+++ QW +S E+ + W P +G M ++ E A S L AL L+ HL + T+L
Sbjct: 92 AQIEQWIDFSSLEIYASILRWFGPRMGFMPYSAPAEEGAISTLKRALDALNTHLTSNTYL 151
Query: 432 VTERITCRCHCLQYTAAC-FPARARPDVRSSLINVQRWFLTVAHQPQVSAVVGSLTLCAA 608
V IT F S +V+R+F TV +QP + V+G + A
Sbjct: 152 VGHSITLADIITVCNLNLGFATVMTKKFTSEFPHVERYFWTVVNQPNFTKVLGDVKQTEA 211
Query: 609 PPTYDPKK 632
P KK
Sbjct: 212 VPPIASKK 219
>At1g02930.1 68414.m00260 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]
Length = 208
Score = 44.4 bits (100), Expect = 8e-05
Identities = 28/82 (34%), Positives = 42/82 (51%), Gaps = 1/82 (1%)
Frame = +1
Query: 1 YTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVL 180
+ +P + + LIA D + + +K E F+ + P GKVPAFE D K+
Sbjct: 7 FGHPASTATRRVLIALHEKNVDFEFVHVELKDGEHKKEPFILRNPFGKVPAFEDGDFKIF 66
Query: 181 LTESNAIAYYVANE-SLRGGDL 243
ES AI Y+A+E S +G +L
Sbjct: 67 --ESRAITQYIAHEFSDKGNNL 86
>At4g02520.1 68417.m00345 glutathione S-transferase, putative
Length = 212
Score = 43.2 bits (97), Expect = 2e-04
Identities = 25/73 (34%), Positives = 38/73 (52%)
Frame = +1
Query: 1 YTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVL 180
+ +P + + LIA D ++ + +K E FL + P G+VPAFE DG +
Sbjct: 7 FGHPASIATRRVLIALHEKNLDFELVHVELKDGEHKKEPFLSRNPFGQVPAFE--DGDLK 64
Query: 181 LTESNAIAYYVAN 219
L ES AI Y+A+
Sbjct: 65 LFESRAITQYIAH 77
>At2g02930.1 68415.m00241 glutathione S-transferase, putative
Length = 212
Score = 42.3 bits (95), Expect = 3e-04
Identities = 25/73 (34%), Positives = 38/73 (52%)
Frame = +1
Query: 1 YTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVL 180
+ +P + + LIA D ++ + +K E FL + P G+VPAFE DG +
Sbjct: 7 FGHPASTSTRRVLIALHEKNLDFELVHVELKDGEHKKEPFLSRNPFGQVPAFE--DGDLK 64
Query: 181 LTESNAIAYYVAN 219
L ES AI Y+A+
Sbjct: 65 LFESRAITQYIAH 77
>At1g02920.1 68414.m00259 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]; supported by cDNA GI:443697.
Length = 209
Score = 40.3 bits (90), Expect = 0.001
Identities = 25/73 (34%), Positives = 36/73 (49%)
Frame = +1
Query: 1 YTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVL 180
+ +P + + LIA D + + +K E F+ + P GKVPAFE DG
Sbjct: 7 FGHPASTATRRVLIALHEKNLDFEFVHIELKDGEHKKEPFIFRNPFGKVPAFE--DGDFK 64
Query: 181 LTESNAIAYYVAN 219
L ES AI Y+A+
Sbjct: 65 LFESRAITQYIAH 77
>At1g02950.2 68414.m00263 glutathione S-transferase, putative
similar to glutathione-S-transferase GI:169887 from
[Silene vulgaris]
Length = 245
Score = 38.3 bits (85), Expect = 0.005
Identities = 24/48 (50%), Positives = 28/48 (58%), Gaps = 1/48 (2%)
Frame = +1
Query: 103 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN-ESLRGGDL 243
+K+E FL P G+VP FE DG V L ES AI Y+A S RG L
Sbjct: 64 HKTEPFLSLNPFGQVPVFE--DGSVKLYESRAITQYIAYVHSSRGTQL 109
>At1g02950.1 68414.m00262 glutathione S-transferase, putative
similar to glutathione-S-transferase GI:169887 from
[Silene vulgaris]
Length = 243
Score = 38.3 bits (85), Expect = 0.005
Identities = 24/48 (50%), Positives = 28/48 (58%), Gaps = 1/48 (2%)
Frame = +1
Query: 103 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN-ESLRGGDL 243
+K+E FL P G+VP FE DG V L ES AI Y+A S RG L
Sbjct: 62 HKTEPFLSLNPFGQVPVFE--DGSVKLYESRAITQYIAYVHSSRGTQL 107
>At2g02380.1 68415.m00176 glutathione S-transferase, putative
similar to gi:167970 gb:AAA72320 gb:AY052332
Length = 223
Score = 37.9 bits (84), Expect = 0.007
Identities = 26/72 (36%), Positives = 38/72 (52%), Gaps = 1/72 (1%)
Frame = +1
Query: 1 YTYPENFRAYKALIAAQYSGTDVKVAP-NFVFGETNKSEDFLKKFPAGKVPAFESADGKV 177
Y+Y + A++ IA G D + P N + G+ + S DF K P G VPA DG V
Sbjct: 15 YSYWRSSCAHRVRIALTLKGLDYEYIPVNLLKGDQSDS-DFKKINPMGTVPAL--VDGDV 71
Query: 178 LLTESNAIAYYV 213
++ +S AI Y+
Sbjct: 72 VINDSFAIIMYL 83
>At1g02940.1 68414.m00261 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]
Length = 256
Score = 37.5 bits (83), Expect = 0.009
Identities = 26/72 (36%), Positives = 36/72 (50%)
Frame = +1
Query: 1 YTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVL 180
Y Y N R A++ + D + N + G+ K FL P G+VP F DG +
Sbjct: 44 YPYSTNTRRVLAVLHEKGLSYD-PITVNLIAGD-QKKPSFLAINPFGQVPVF--LDGGLK 99
Query: 181 LTESNAIAYYVA 216
LTES AI+ Y+A
Sbjct: 100 LTESRAISEYIA 111
>At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1)
(GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
(Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
{Arabidopsis thaliana}; contains Pfam profiles PF02798:
Glutathione S-transferase, N-terminal domain and
PF00043:Glutathione S-transferase, C-terminal domain
Length = 191
Score = 36.3 bits (80), Expect = 0.022
Identities = 26/72 (36%), Positives = 37/72 (51%), Gaps = 1/72 (1%)
Frame = +1
Query: 1 YTYPENFRAYKALIAAQYSGTDVKVAP-NFVFGETNKSEDFLKKFPAGKVPAFESADGKV 177
Y+Y + A++ IA G D + P N + G+ S DF K P G VPA DG V
Sbjct: 12 YSYWRSSCAHRVRIALALKGLDYEYIPVNLLKGDQFDS-DFKKINPMGTVPAL--VDGDV 68
Query: 178 LLTESNAIAYYV 213
++ +S AI Y+
Sbjct: 69 VINDSFAIIMYL 80
>At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1)
(GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
(Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
{Arabidopsis thaliana}; contains Pfam profiles PF02798:
Glutathione S-transferase, N-terminal domain and
PF00043:Glutathione S-transferase, C-terminal domain
Length = 221
Score = 36.3 bits (80), Expect = 0.022
Identities = 26/72 (36%), Positives = 37/72 (51%), Gaps = 1/72 (1%)
Frame = +1
Query: 1 YTYPENFRAYKALIAAQYSGTDVKVAP-NFVFGETNKSEDFLKKFPAGKVPAFESADGKV 177
Y+Y + A++ IA G D + P N + G+ S DF K P G VPA DG V
Sbjct: 12 YSYWRSSCAHRVRIALALKGLDYEYIPVNLLKGDQFDS-DFKKINPMGTVPAL--VDGDV 68
Query: 178 LLTESNAIAYYV 213
++ +S AI Y+
Sbjct: 69 VINDSFAIIMYL 80
>At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1)
(GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
(Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
{Arabidopsis thaliana}; contains Pfam profiles PF02798:
Glutathione S-transferase, N-terminal domain and
PF00043:Glutathione S-transferase, C-terminal domain
Length = 228
Score = 33.1 bits (72), Expect = 0.20
Identities = 26/78 (33%), Positives = 38/78 (48%), Gaps = 7/78 (8%)
Frame = +1
Query: 1 YTYPENFRAYKALIAAQYSGTDVKVAP-NFVFGETNKS------EDFLKKFPAGKVPAFE 159
Y+Y + A++ IA G D + P N + G+ S +DF K P G VPA
Sbjct: 12 YSYWRSSCAHRVRIALALKGLDYEYIPVNLLKGDQFDSVYRFDLQDFKKINPMGTVPAL- 70
Query: 160 SADGKVLLTESNAIAYYV 213
DG V++ +S AI Y+
Sbjct: 71 -VDGDVVINDSFAIIMYL 87
>At2g28290.2 68415.m03434 chromatin remodeling protein, putative (SYD)
similar to transcriptional activator HBRM [Homo sapiens]
GI:414117; contains Pfam profiles PF00271: Helicase
conserved C-terminal domain, PF00176: SNF2 family
N-terminal domain; identical to cDNA putative chromatin
remodeling protein SYD (SPLAYED) GI:13603720
Length = 3529
Score = 29.9 bits (64), Expect = 1.9
Identities = 16/44 (36%), Positives = 23/44 (52%)
Frame = -2
Query: 330 QGKGRPKRRKQAVVRCQTMMPTARHGLGSQISSAETFIGNVVSD 199
+G+GRPKR +A+ + G+ ISSA T + V SD
Sbjct: 1449 RGRGRPKRTDKALTPVSLSAVSRTQATGNAISSAATGLDFVSSD 1492
>At2g28290.1 68415.m03433 chromatin remodeling protein, putative (SYD)
similar to transcriptional activator HBRM [Homo sapiens]
GI:414117; contains Pfam profiles PF00271: Helicase
conserved C-terminal domain, PF00176: SNF2 family
N-terminal domain; identical to cDNA putative chromatin
remodeling protein SYD (SPLAYED) GI:13603720
Length = 3574
Score = 29.9 bits (64), Expect = 1.9
Identities = 16/44 (36%), Positives = 23/44 (52%)
Frame = -2
Query: 330 QGKGRPKRRKQAVVRCQTMMPTARHGLGSQISSAETFIGNVVSD 199
+G+GRPKR +A+ + G+ ISSA T + V SD
Sbjct: 1449 RGRGRPKRTDKALTPVSLSAVSRTQATGNAISSAATGLDFVSSD 1492
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 29.9 bits (64), Expect = 1.9
Identities = 20/66 (30%), Positives = 33/66 (50%)
Frame = +3
Query: 384 AALKVLDGHLLTRTFLVTERITCRCHCLQYTAACFPARARPDVRSSLINVQRWFLTVAHQ 563
+ LK LD HLLTR+++ + + + F A ++P S +NV RWF +
Sbjct: 11 SGLKKLDEHLLTRSYITGYQAS------KDDITVFTALSKPPT-SEFVNVSRWFNHIDAL 63
Query: 564 PQVSAV 581
++S V
Sbjct: 64 LRISGV 69
>At5g46550.1 68418.m05731 DNA-binding bromodomain-containing protein
contains bromodomain, INTERPRO:IPR001487
Length = 494
Score = 29.5 bits (63), Expect = 2.5
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = +1
Query: 34 ALIAAQYSGTDVKVAPNFVFGETNKSE 114
A++ AQY+GT +K V G+ NK++
Sbjct: 342 AILKAQYAGTIIKAKHRIVLGQNNKAD 368
>At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N
domain-containing protein-related contains weak hit to
Pfam F00627: UBA/TS-N domain; supported by tandem
duplication of ubiquitin-associated (UBA)/TS-N domain
protein (GI:2924790) (TIGR_Ath1:At2g33010) [Arabidopsis
thaliana]
Length = 508
Score = 29.5 bits (63), Expect = 2.5
Identities = 12/40 (30%), Positives = 19/40 (47%)
Frame = +1
Query: 328 LVSCNSTNRMLNVQSLTYWPP*KYWTDIFSHAPSLLPRES 447
L++ + N N +S + WPP K W D H + E+
Sbjct: 195 LMASSQVNTQENQESTSVWPPMKTWVDRICHETKVSREEA 234
>At1g16900.1 68414.m02047 curculin-like (mannose-binding) lectin
family protein very low similarity to Ser Thr protein
kinase GI:2598067 from (Zea mays); contains Pfam lectin
(probable mannose binding) domain PF01453
Length = 919
Score = 29.5 bits (63), Expect = 2.5
Identities = 16/44 (36%), Positives = 22/44 (50%)
Frame = +2
Query: 236 EIWLPKPCLAVGIMV*QRTTACFLRLGLPLPWYHAIQQTEC*TC 367
E WLP C A GI + AC L +GL + W A + + +C
Sbjct: 276 ECWLPSKCGAFGICEDNQCVACPLGVGL-MGWSKACKPKKVKSC 318
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 29.1 bits (62), Expect = 3.3
Identities = 20/66 (30%), Positives = 32/66 (48%)
Frame = +3
Query: 384 AALKVLDGHLLTRTFLVTERITCRCHCLQYTAACFPARARPDVRSSLINVQRWFLTVAHQ 563
A LK LD HLLTR+++ + + + F A A+P S +N RW+ +
Sbjct: 11 AGLKKLDEHLLTRSYITGYQAS------KDDITVFAALAKPPT-SQYVNASRWYNHIDAL 63
Query: 564 PQVSAV 581
++S V
Sbjct: 64 LRISGV 69
>At4g22130.1 68417.m03199 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 338
Score = 28.7 bits (61), Expect = 4.3
Identities = 21/74 (28%), Positives = 35/74 (47%), Gaps = 6/74 (8%)
Frame = +1
Query: 22 RAYKALIAAQYSGTDVKVAPNF-----VFGETNKSEDFLKKFPAGKVPAFESADGKVL-L 183
R + A+QY+ + ++VA N + GE + + +FP GK+ A + D L L
Sbjct: 8 RIRSPITASQYTVSSLQVATNSFSQENIIGEGSLGRVYRAEFPNGKIMAIKKIDNAALSL 67
Query: 184 TESNAIAYYVANES 225
E + V+N S
Sbjct: 68 QEEDNFLEAVSNMS 81
>At1g27850.1 68414.m03413 expressed protein similar to En/Spm-like
transposon protein GB:AAB95292 GI:2088658 from
[Arabidopsis thaliana]
Length = 1148
Score = 27.9 bits (59), Expect = 7.6
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = -2
Query: 591 ASRRRPTLAAGGRRSGTNAERLSATNGR 508
+S R P+ A GRRSGT R+S T G+
Sbjct: 171 SSARHPS-PASGRRSGTPVRRISPTPGK 197
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,730,870
Number of Sequences: 28952
Number of extensions: 365725
Number of successful extensions: 1057
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 1026
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1057
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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