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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30625.Seq
         (698 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g53480.1 68418.m06646 importin beta-2, putative similar to im...    50   2e-06
At2g32810.1 68415.m04016 beta-galactosidase, putative / lactase,...    31   0.56 
At3g25840.1 68416.m03219 protein kinase family protein contains ...    30   1.7  
At3g59990.2 68416.m06698 methionyl aminopeptidase, putative / me...    29   3.9  
At3g59990.1 68416.m06697 methionyl aminopeptidase, putative / me...    29   3.9  
At2g44180.1 68415.m05496 methionyl aminopeptidase, putative / me...    29   3.9  
At5g47690.1 68418.m05887 expressed protein                             28   6.8  
At1g08730.1 68414.m00969 myosin heavy chain (PCR43) identical to...    28   6.8  

>At5g53480.1 68418.m06646 importin beta-2, putative similar to
           importin-beta2 [Oryza sativa (japonica cultivar-group)]
           GI:3983665; contains Pfam profile PF03810: Importin-beta
           N-terminal domain
          Length = 870

 Score = 49.6 bits (113), Expect = 2e-06
 Identities = 35/121 (28%), Positives = 57/121 (47%), Gaps = 1/121 (0%)
 Frame = +3

Query: 267 ARMAAGLQLKNHLTSKDPTLKQQYQQRWLALAEDVRLYIKENILAAIGTE-NSRPSSAAQ 443
           +R  AGL LKN L +K+   K +  QRWLAL    +  I+  +L  +        S+A+Q
Sbjct: 55  SRKLAGLVLKNALDAKEQHRKYELVQRWLALDMSTKSQIRAFLLKTLSAPVPDVRSTASQ 114

Query: 444 CVAYVGSS*APSRTMGMI*YQFCRKCCSCPIY*TQKEATLEAIGLHCQDIDAEV*QKEVS 623
            +A V     P +    +           P +   K+ATLE +G  C+++  +V ++E  
Sbjct: 115 VIAKVAGIELPQKQWPELIVSLLSNIHQLPAH--VKQATLETLGYLCEEVSPDVVEQEHV 172

Query: 624 N 626
           N
Sbjct: 173 N 173


>At2g32810.1 68415.m04016 beta-galactosidase, putative / lactase,
           putative similar to beta-galactosidase GI:7939617 from
           [Lycopersicon esculentum]
          Length = 887

 Score = 31.5 bits (68), Expect = 0.56
 Identities = 21/81 (25%), Positives = 34/81 (41%)
 Frame = +1

Query: 412 QRIVVQVQQHSVWRMWAVAELPVGQWE*SNTNFVENVVHVQSTELKRKRLWRLLGYIVRI 591
           Q++V Q     + + W   + P+G W  +N  F   + H+  T+ +   LW      V  
Sbjct: 456 QKVVRQDNVSYISKSWMALKEPIGIWGENNFTFQGLLEHLNVTKDRSDYLWHKTRISVSE 515

Query: 592 LMLKFNRKKYPIPTGIIHGMR 654
             + F +K  P  T  I  MR
Sbjct: 516 DDISFWKKNGPNSTVSIDSMR 536


>At3g25840.1 68416.m03219 protein kinase family protein contains
           Pfam profile: PF00069 eukaryotic protein kinase domain
          Length = 935

 Score = 29.9 bits (64), Expect = 1.7
 Identities = 14/37 (37%), Positives = 22/37 (59%)
 Frame = +1

Query: 76  LKNSETMHAETTLTLIQILEKTVSPDRNELEAAVRYL 186
           ++N+ETMH    +  +QIL+K    DR +    VR+L
Sbjct: 652 IRNNETMHKAGKIE-VQILKKLAGADREDRRHCVRFL 687


>At3g59990.2 68416.m06698 methionyl aminopeptidase, putative /
           methionine aminopeptidase, putative / peptidase M,
           putative similar to Methionine aminopeptidase 2 (EC
           3.4.11.18) from {Rattus norvegicus} SP|P38062, {Homo
           sapiens} SP|P50579; contains Pfam profile PF00557:
           metallopeptidase family M24; supporting cDNA
           gi|11344921|gb|AF300880.1|AF300880
          Length = 439

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 16/56 (28%), Positives = 25/56 (44%)
 Frame = +1

Query: 70  HKLKNSETMHAETTLTLIQILEKTVSPDRNELEAAVRYLDHAATTNFTTFIKMLSD 237
           H +KN +  H    L   + L  T++ + + L    RYLD    T +   +K L D
Sbjct: 343 HYMKNFDAGHVPLRLPRAKQLLATINKNFSTLAFCRRYLDRIGETKYLMALKNLCD 398


>At3g59990.1 68416.m06697 methionyl aminopeptidase, putative /
           methionine aminopeptidase, putative / peptidase M,
           putative similar to Methionine aminopeptidase 2 (EC
           3.4.11.18) from {Rattus norvegicus} SP|P38062, {Homo
           sapiens} SP|P50579; contains Pfam profile PF00557:
           metallopeptidase family M24; supporting cDNA
           gi|11344921|gb|AF300880.1|AF300880
          Length = 439

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 16/56 (28%), Positives = 25/56 (44%)
 Frame = +1

Query: 70  HKLKNSETMHAETTLTLIQILEKTVSPDRNELEAAVRYLDHAATTNFTTFIKMLSD 237
           H +KN +  H    L   + L  T++ + + L    RYLD    T +   +K L D
Sbjct: 343 HYMKNFDAGHVPLRLPRAKQLLATINKNFSTLAFCRRYLDRIGETKYLMALKNLCD 398


>At2g44180.1 68415.m05496 methionyl aminopeptidase, putative /
           methionine aminopeptidase, putative / peptidase M,
           putative similar to SP|P50579 Methionine aminopeptidase
           2 (EC 3.4.11.18) (MetAP 2) {Homo sapiens}; contains Pfam
           profile PF00557: metallopeptidase family M24
          Length = 441

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 16/56 (28%), Positives = 25/56 (44%)
 Frame = +1

Query: 70  HKLKNSETMHAETTLTLIQILEKTVSPDRNELEAAVRYLDHAATTNFTTFIKMLSD 237
           H +KN +  H    L   + L  T++ + + L    RYLD    T +   +K L D
Sbjct: 345 HYMKNYDVGHVPLRLPRAKQLLATINKNFSTLAFCRRYLDRLGETKYLMALKNLCD 400


>At5g47690.1 68418.m05887 expressed protein
          Length = 1638

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 14/41 (34%), Positives = 22/41 (53%)
 Frame = +3

Query: 207  FHNIHQNVVRCPSPRWNSQVARMAAGLQLKNHLTSKDPTLK 329
            FH+I Q+     + +  +  A    GL + NHLT K+P L+
Sbjct: 1031 FHSIKQSEDVTDATKSKNSHAICELGLSIINHLTQKEPDLQ 1071


>At1g08730.1 68414.m00969 myosin heavy chain (PCR43) identical to
            myosin heavy chain PCR43 (PIR:T00727) [Arabidopsis
            thaliana]; similar to ESTs gb|R30087 and gb|AA394762
          Length = 1538

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 16/45 (35%), Positives = 21/45 (46%)
 Frame = -3

Query: 687  AKSNMITGFSGPHAMYDARRNWILLSVKLQHQYPDNVAQ*PPKSL 553
            ++S +  G    H   DAR N  L S  + H+ P  V   P KSL
Sbjct: 1073 SRSILQRGSESGHLAVDARSNLDLHSHSINHRDPSEVEDKPQKSL 1117


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,217,902
Number of Sequences: 28952
Number of extensions: 311592
Number of successful extensions: 873
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 855
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 872
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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