BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30618.Seq (797 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1906.01 |mpg1||mannose-1-phosphate guanyltransferase Mpg1|Sc... 126 4e-30 SPBC13G1.02 |||mannose-1-phosphate guanyltransferase |Schizosacc... 53 4e-08 SPBC1711.12 |||serine peptidase |Schizosaccharomyces pombe|chr 2... 34 0.021 SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon... 31 0.19 SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p... 25 9.5 SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyce... 25 9.5 SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces pomb... 25 9.5 >SPCC1906.01 |mpg1||mannose-1-phosphate guanyltransferase Mpg1|Schizosaccharomyces pombe|chr 3|||Manual Length = 363 Score = 126 bits (304), Expect = 4e-30 Identities = 60/90 (66%), Positives = 72/90 (80%), Gaps = 3/90 (3%) Frame = +3 Query: 510 VICEFPFKELARYHKNHGKEGTIVVTKVEEPSKYGVVVYDDNGQ--IESFIEKPQEFISN 683 VICE+PF +LA +HK HG EGTIVVTKVEEPSKYGVVV+ N + IE F+EKP EF+SN Sbjct: 111 VICEYPFADLAAFHKAHGAEGTIVVTKVEEPSKYGVVVHYPNSESLIERFVEKPVEFVSN 170 Query: 684 KINAGMYLLNPSVLSRIELRPMSLRRS-FP 770 +IN G+Y+LNPSVL RIE RP S+ + FP Sbjct: 171 RINGGIYILNPSVLDRIEPRPTSIEKEVFP 200 Score = 94.3 bits (224), Expect = 2e-20 Identities = 43/78 (55%), Positives = 58/78 (74%) Frame = +1 Query: 256 VEFANKPILMHQIEALVEAGVTQVILAVSYRAEEMEKELTEQVSKLGVSLTFSHETEPLG 435 VEF NKP+++HQ+EAL AGVT ++LAV+YR E M + L + + V++TFS E EPLG Sbjct: 26 VEFGNKPMILHQVEALAAAGVTDIVLAVNYRPEIMVEALKKYEKEYNVNITFSVENEPLG 85 Query: 436 TAGPLALARELLSTSSEP 489 TAGPLALAR++L+ P Sbjct: 86 TAGPLALARDILAKDHSP 103 Score = 44.8 bits (101), Expect = 1e-05 Identities = 19/24 (79%), Positives = 23/24 (95%) Frame = +2 Query: 182 IRALILVGGYGTRLRPLTLSRPKP 253 ++ALILVGG+GTRLRPLTL+ PKP Sbjct: 1 MKALILVGGFGTRLRPLTLTLPKP 24 >SPBC13G1.02 |||mannose-1-phosphate guanyltransferase |Schizosaccharomyces pombe|chr 2|||Manual Length = 414 Score = 53.2 bits (122), Expect = 4e-08 Identities = 25/79 (31%), Positives = 46/79 (58%), Gaps = 3/79 (3%) Frame = +3 Query: 510 VICEFPFKELARYHKNHGKEGTIVVTKV--EEPSKYGVVVYDDN-GQIESFIEKPQEFIS 680 V C FP +EL H T++ TKV E+ S +G +V + + G++ +++KP ++S Sbjct: 119 VCCSFPLQELLNVHHEKKALVTLMATKVSKEDASNFGCLVEEPSTGRVLHYVDKPSSYLS 178 Query: 681 NKINAGMYLLNPSVLSRIE 737 N I+ G+Y+ + S+ I+ Sbjct: 179 NIISCGIYIFDASIFDEIK 197 Score = 29.5 bits (63), Expect = 0.58 Identities = 15/24 (62%), Positives = 17/24 (70%), Gaps = 2/24 (8%) Frame = +2 Query: 188 ALILVGG--YGTRLRPLTLSRPKP 253 A+ILVGG GTR RPL+ PKP Sbjct: 5 AVILVGGPSRGTRFRPLSFDVPKP 28 >SPBC1711.12 |||serine peptidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 683 Score = 34.3 bits (75), Expect = 0.021 Identities = 18/48 (37%), Positives = 25/48 (52%) Frame = +3 Query: 597 EPSKYGVVVYDDNGQIESFIEKPQEFISNKINAGMYLLNPSVLSRIEL 740 E + VV + G + FIE F +K N+G+YLLN S + EL Sbjct: 31 EAPRRSAVVSNPLGNLGIFIESNYSFADHKYNSGIYLLNESTRNHQEL 78 >SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 678 Score = 31.1 bits (67), Expect = 0.19 Identities = 14/38 (36%), Positives = 23/38 (60%), Gaps = 3/38 (7%) Frame = +2 Query: 164 DKKLEEIRALILVGGYGTRLRPLTLSRPK---PRSNSP 268 +K ++A++L Y R RPLTL +P+ P +N+P Sbjct: 12 EKPKHALQAIVLSDSYNYRFRPLTLDKPRCLLPLANTP 49 >SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1323 Score = 25.4 bits (53), Expect = 9.5 Identities = 13/36 (36%), Positives = 15/36 (41%), Gaps = 1/36 (2%) Frame = -3 Query: 519 HKLHRVQYKEWFRTGAEQFPGKCQWSC-CSQWFSLM 415 H +H YKEWF G + C C C F M Sbjct: 1284 HVMHEDCYKEWFSNG-DSISQSCSSGCGCKCQFQHM 1318 >SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 844 Score = 25.4 bits (53), Expect = 9.5 Identities = 11/22 (50%), Positives = 16/22 (72%) Frame = -2 Query: 541 ASSLNGNSQITQSSIQRMVPNW 476 AS + +S+ +SSIQR +PNW Sbjct: 794 ASIILLDSRYNRSSIQRKLPNW 815 >SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces pombe|chr 2|||Manual Length = 2386 Score = 25.4 bits (53), Expect = 9.5 Identities = 10/24 (41%), Positives = 16/24 (66%) Frame = +2 Query: 2 SKQNLCLRTYIHTPLETNLAECAY 73 +++NLC+RTY+ PL EC + Sbjct: 2112 NRRNLCIRTYVVIPLN---EECGF 2132 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,129,061 Number of Sequences: 5004 Number of extensions: 60952 Number of successful extensions: 164 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 152 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 162 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 389395636 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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