Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30545.Seq
(748 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q17GU0 Cluster: Putative uncharacterized protein; n=1; ... 95 2e-18
UniRef50_UPI0000DB6E45 Cluster: PREDICTED: similar to CG6962-PA;... 88 2e-16
UniRef50_Q7QFC4 Cluster: ENSANGP00000010147; n=1; Anopheles gamb... 66 7e-10
UniRef50_Q9NXE4-4 Cluster: Isoform 4 of Q9NXE4 ; n=1; Homo sapie... 64 3e-09
UniRef50_Q9NXE4 Cluster: Sphingomyelin phosphodiesterase 4; n=32... 64 3e-09
UniRef50_A7SAI1 Cluster: Predicted protein; n=1; Nematostella ve... 64 5e-09
UniRef50_UPI0000E47E3F Cluster: PREDICTED: similar to neutral sp... 61 2e-08
UniRef50_Q6PFJ7 Cluster: Sphingomyelin phosphodiesterase 4; n=6;... 60 6e-08
UniRef50_Q4T200 Cluster: Chromosome undetermined SCAF10406, whol... 57 4e-07
UniRef50_Q4JC25 Cluster: Conserved Archaeal protein; n=1; Sulfol... 35 2.4
UniRef50_A2EEQ4 Cluster: Ankyrin repeat protein, putative; n=1; ... 34 4.3
UniRef50_A4SAJ3 Cluster: Predicted protein; n=1; Ostreococcus lu... 33 5.6
UniRef50_Q5C353 Cluster: SJCHGC04234 protein; n=1; Schistosoma j... 33 5.6
UniRef50_Q6CNT5 Cluster: Kluyveromyces lactis strain NRRL Y-1140... 33 5.6
UniRef50_Q4UHH4 Cluster: Putative uncharacterized protein; n=3; ... 33 7.5
UniRef50_Q59TI0 Cluster: Hypothetical LPF family protein 31; n=2... 33 7.5
UniRef50_Q96A72 Cluster: Protein mago nashi homolog 2; n=89; Euk... 33 7.5
>UniRef50_Q17GU0 Cluster: Putative uncharacterized protein; n=1;
Aedes aegypti|Rep: Putative uncharacterized protein -
Aedes aegypti (Yellowfever mosquito)
Length = 765
Score = 95.1 bits (226), Expect = 2e-18
Identities = 51/131 (38%), Positives = 81/131 (61%), Gaps = 1/131 (0%)
Frame = +2
Query: 263 ELTRIIDQSSSTKDLQTIFPQLINNIFSSSFSTGWDLKSITCDVNRYEFEALISFLEP-Q 439
EL +ID++S K+LQ I+P LIN+IF + +GW + +T D + ++F+ L +F P
Sbjct: 28 ELGLLIDRAS-LKELQDIYPTLINSIFGLNGGSGWGFRVMTRDTHPHDFDVLYNFFIPIG 86
Query: 440 GPMFRLCYRLLSDTQLKYELPLNYYLLIYR*H*KEGDAHNFMADMLTADAQTMNMDFFGT 619
GPMFR+CYRLL+D+ LKYELP++Y + + G F +D++ D +
Sbjct: 87 GPMFRMCYRLLNDS-LKYELPISYLPPKMQQVIESGRYSAFYSDIINIDPFRRQVVSLSL 145
Query: 620 QSFDYYIFNFA 652
+FDY++F+FA
Sbjct: 146 NAFDYFMFHFA 156
>UniRef50_UPI0000DB6E45 Cluster: PREDICTED: similar to CG6962-PA;
n=2; Apocrita|Rep: PREDICTED: similar to CG6962-PA -
Apis mellifera
Length = 744
Score = 88.2 bits (209), Expect = 2e-16
Identities = 46/111 (41%), Positives = 66/111 (59%)
Frame = +2
Query: 263 ELTRIIDQSSSTKDLQTIFPQLINNIFSSSFSTGWDLKSITCDVNRYEFEALISFLEPQG 442
E+ +ID+SS+T +LQ +F LI+++F + + GW L SIT N E+E L +FL PQG
Sbjct: 26 EIAMLIDESSTT-ELQHVFSILIDSLFGITDNIGWGLHSITFKKNAQEYETLCNFLNPQG 84
Query: 443 PMFRLCYRLLSDTQLKYELPLNYYLLIYR*H*KEGDAHNFMADMLTADAQT 595
P+F LCY+LL D LKY P+++ R +EG F D + D T
Sbjct: 85 PVFSLCYKLLPDCYLKYNFPVSFLPAKIRLMLEEGVIPPFYLDKIRDDQST 135
>UniRef50_Q7QFC4 Cluster: ENSANGP00000010147; n=1; Anopheles gambiae
str. PEST|Rep: ENSANGP00000010147 - Anopheles gambiae
str. PEST
Length = 452
Score = 66.5 bits (155), Expect = 7e-10
Identities = 44/131 (33%), Positives = 67/131 (51%), Gaps = 1/131 (0%)
Frame = +2
Query: 263 ELTRIIDQSSSTKDLQTIFPQLINNIFSSSFSTGWDLKSITCDVNRYEFEALISFLEP-Q 439
E R I + S DL +FP LI++IF S GW+L+ IT + EF+ L F P +
Sbjct: 26 EELRTIFEHESVHDLHDMFPLLISSIFDLS-GPGWNLRKITRENAPREFDVLQEFFSPLR 84
Query: 440 GPMFRLCYRLLSDTQLKYELPLNYYLLIYR*H*KEGDAHNFMADMLTADAQTMNMDFFGT 619
GPM RLCY+L+ + KYE+P+ Y + + G F + + D + +
Sbjct: 85 GPMIRLCYKLID--RNKYEVPIAYLPVKMQHMLLAGLYPPFYSKRIRIDPFSRTVVGLTL 142
Query: 620 QSFDYYIFNFA 652
SF+Y++ FA
Sbjct: 143 NSFEYFLLFFA 153
>UniRef50_Q9NXE4-4 Cluster: Isoform 4 of Q9NXE4 ; n=1; Homo
sapiens|Rep: Isoform 4 of Q9NXE4 - Homo sapiens (Human)
Length = 873
Score = 64.1 bits (149), Expect = 3e-09
Identities = 31/91 (34%), Positives = 56/91 (61%), Gaps = 2/91 (2%)
Frame = +2
Query: 245 FTREVIELTRIIDQSSSTKDLQTIFPQLINNIFSS--SFSTGWDLKSITCDVNRYEFEAL 418
F ++ +L ++I+ + K+L TIFP L+ +IF S GW+L+ + VN E+ +
Sbjct: 25 FAQQCQDLVKVIEDFPA-KELHTIFPWLVESIFGSLDGVLVGWNLRCLQGRVNPVEYSIV 83
Query: 419 ISFLEPQGPMFRLCYRLLSDTQLKYELPLNY 511
+ FL+P GPM +L Y+L ++ K++ P++Y
Sbjct: 84 MEFLDPGGPMMKLVYKLQAE-DYKFDFPVSY 113
>UniRef50_Q9NXE4 Cluster: Sphingomyelin phosphodiesterase 4; n=32;
Tetrapoda|Rep: Sphingomyelin phosphodiesterase 4 - Homo
sapiens (Human)
Length = 827
Score = 64.1 bits (149), Expect = 3e-09
Identities = 31/91 (34%), Positives = 56/91 (61%), Gaps = 2/91 (2%)
Frame = +2
Query: 245 FTREVIELTRIIDQSSSTKDLQTIFPQLINNIFSS--SFSTGWDLKSITCDVNRYEFEAL 418
F ++ +L ++I+ + K+L TIFP L+ +IF S GW+L+ + VN E+ +
Sbjct: 25 FAQQCQDLVKVIEDFPA-KELHTIFPWLVESIFGSLDGVLVGWNLRCLQGRVNPVEYSIV 83
Query: 419 ISFLEPQGPMFRLCYRLLSDTQLKYELPLNY 511
+ FL+P GPM +L Y+L ++ K++ P++Y
Sbjct: 84 MEFLDPGGPMMKLVYKLQAE-DYKFDFPVSY 113
>UniRef50_A7SAI1 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 737
Score = 63.7 bits (148), Expect = 5e-09
Identities = 34/78 (43%), Positives = 45/78 (57%)
Frame = +2
Query: 263 ELTRIIDQSSSTKDLQTIFPQLINNIFSSSFSTGWDLKSITCDVNRYEFEALISFLEPQG 442
E++R+ID SS K+L FP L+ ++F W L++I N EFE L FL P G
Sbjct: 22 EISRLIDDSS-IKELHNFFPDLLGSVFGYDGKPDWGLRTIL--PNHREFEVLRLFLSPHG 78
Query: 443 PMFRLCYRLLSDTQLKYE 496
P+FRL +L D LKYE
Sbjct: 79 PLFRLLDKLQVDGHLKYE 96
>UniRef50_UPI0000E47E3F Cluster: PREDICTED: similar to neutral
sphingomyelinase 3, partial; n=1; Strongylocentrotus
purpuratus|Rep: PREDICTED: similar to neutral
sphingomyelinase 3, partial - Strongylocentrotus
purpuratus
Length = 874
Score = 61.3 bits (142), Expect = 2e-08
Identities = 37/130 (28%), Positives = 63/130 (48%)
Frame = +2
Query: 263 ELTRIIDQSSSTKDLQTIFPQLINNIFSSSFSTGWDLKSITCDVNRYEFEALISFLEPQG 442
EL +I +SSS KDL +I+P ++ +F + GW L ++ + + + L F+ P G
Sbjct: 19 ELESLIRRSSS-KDLNSIYPLFLDLVFGLNGHPGWGLNTLNSLYAKDDSKHLQDFMSPSG 77
Query: 443 PMFRLCYRLLSDTQLKYELPLNYYLLIYR*H*KEGDAHNFMADMLTADAQTMNMDFFGTQ 622
P+F + Y+L SD L+YE P++ R G F L +
Sbjct: 78 PVFLMVYKLQSDHYLRYEFPVDKLSAPTRASLASGIVPPFYQGKLNYQSHGRAPSVVMLN 137
Query: 623 SFDYYIFNFA 652
+F+Y+++ FA
Sbjct: 138 AFEYFMYVFA 147
>UniRef50_Q6PFJ7 Cluster: Sphingomyelin phosphodiesterase 4; n=6;
Euteleostomi|Rep: Sphingomyelin phosphodiesterase 4 -
Danio rerio (Zebrafish) (Brachydanio rerio)
Length = 791
Score = 60.1 bits (139), Expect = 6e-08
Identities = 32/85 (37%), Positives = 50/85 (58%), Gaps = 2/85 (2%)
Frame = +2
Query: 263 ELTRIIDQSSSTKDLQTIFPQLINNIFSS--SFSTGWDLKSITCDVNRYEFEALISFLEP 436
EL +IID + K+L IFP L+ +F S TGW+L+ + E+ + FL+P
Sbjct: 31 ELCKIIDDYPA-KELHAIFPWLVECVFGSLDGILTGWNLRFL--QARSAEYSIAMEFLDP 87
Query: 437 QGPMFRLCYRLLSDTQLKYELPLNY 511
GPM +L Y+L ++ + KYE P++Y
Sbjct: 88 SGPMMKLVYKLQAE-EYKYEFPISY 111
>UniRef50_Q4T200 Cluster: Chromosome undetermined SCAF10406, whole
genome shotgun sequence; n=3; Clupeocephala|Rep:
Chromosome undetermined SCAF10406, whole genome shotgun
sequence - Tetraodon nigroviridis (Green puffer)
Length = 825
Score = 57.2 bits (132), Expect = 4e-07
Identities = 29/85 (34%), Positives = 51/85 (60%), Gaps = 2/85 (2%)
Frame = +2
Query: 263 ELTRIIDQSSSTKDLQTIFPQLINNIFSS--SFSTGWDLKSITCDVNRYEFEALISFLEP 436
+L +II+ + K+L +FP L+ ++F S GW+L+ + N Y ++ FL P
Sbjct: 21 DLVKIIEDYPA-KELHPVFPWLVESVFGSLDGVMAGWNLRLLNSRTNDYN--VVMEFLSP 77
Query: 437 QGPMFRLCYRLLSDTQLKYELPLNY 511
GPM +L Y+L ++ + KYE+P++Y
Sbjct: 78 GGPMMKLVYKLQAE-EYKYEVPVSY 101
>UniRef50_Q4JC25 Cluster: Conserved Archaeal protein; n=1;
Sulfolobus acidocaldarius|Rep: Conserved Archaeal
protein - Sulfolobus acidocaldarius
Length = 324
Score = 34.7 bits (76), Expect = 2.4
Identities = 17/44 (38%), Positives = 25/44 (56%)
Frame = +2
Query: 389 DVNRYEFEALISFLEPQGPMFRLCYRLLSDTQLKYELPLNYYLL 520
+ + + F L+ FL+ P RL ++LSD Y+ PLN YLL
Sbjct: 132 EASAHVFHNLVEFLKGDQPRIRLGEKILSDEISVYDNPLNNYLL 175
>UniRef50_A2EEQ4 Cluster: Ankyrin repeat protein, putative; n=1;
Trichomonas vaginalis G3|Rep: Ankyrin repeat protein,
putative - Trichomonas vaginalis G3
Length = 226
Score = 33.9 bits (74), Expect = 4.3
Identities = 15/44 (34%), Positives = 28/44 (63%)
Frame = +2
Query: 137 YENKSLEYIFNHQTKLNK*NNGSRFDESVLYKSEFAFTREVIEL 268
Y+ ++++F+H +++ NG+R DES L + F T E++EL
Sbjct: 110 YQKDMVQFLFSHGVEIS---NGNRDDESSLQSAAFRSTPEIVEL 150
>UniRef50_A4SAJ3 Cluster: Predicted protein; n=1; Ostreococcus
lucimarinus CCE9901|Rep: Predicted protein -
Ostreococcus lucimarinus CCE9901
Length = 954
Score = 33.5 bits (73), Expect = 5.6
Identities = 27/100 (27%), Positives = 44/100 (44%), Gaps = 2/100 (2%)
Frame = +2
Query: 215 ESVLYKSEFAFTREVIELTRIIDQSSSTKDLQTIFPQLINNIFS--SSFSTGWDLKSITC 388
ES SE T E IE ++ + + F ++ +FS S S GW + T
Sbjct: 89 ESANLGSEAMKTIETIERVLKRNRENLREFFSACFQPILCRLFSFDGSSSEGWMNAAFTT 148
Query: 389 DVNRYEFEALISFLEPQGPMFRLCYRLLSDTQLKYELPLN 508
R + + L+ FL P+G + R SD +++ PL+
Sbjct: 149 GSER-DVKDLLDFLSPEGSLIRAMLIADSDKLVQFAFPLD 187
>UniRef50_Q5C353 Cluster: SJCHGC04234 protein; n=1; Schistosoma
japonicum|Rep: SJCHGC04234 protein - Schistosoma
japonicum (Blood fluke)
Length = 238
Score = 33.5 bits (73), Expect = 5.6
Identities = 29/86 (33%), Positives = 40/86 (46%), Gaps = 3/86 (3%)
Frame = +2
Query: 257 VIELTRIIDQSSSTKDLQTIFPQLINNIFS-SSFSTGWDLKSITCDVNRYEFEALISFLE 433
V EL+ I+ + +DL +++NIF S+ + GW L SI Y F + FL
Sbjct: 60 VKELSSILARGK-LQDLHNSVVLIVDNIFGYSNGNDGWALHSIKEADEPYLFGCIREFLS 118
Query: 434 PQGPMFR-LCYRL-LSDTQLKYELPL 505
P G L RL L KY+ PL
Sbjct: 119 PNGRFLEALSSRLTLEFPTCKYDFPL 144
>UniRef50_Q6CNT5 Cluster: Kluyveromyces lactis strain NRRL Y-1140
chromosome E of strain NRRL Y- 1140 of Kluyveromyces
lactis; n=1; Kluyveromyces lactis|Rep: Kluyveromyces
lactis strain NRRL Y-1140 chromosome E of strain NRRL Y-
1140 of Kluyveromyces lactis - Kluyveromyces lactis
(Yeast) (Candida sphaerica)
Length = 338
Score = 33.5 bits (73), Expect = 5.6
Identities = 41/129 (31%), Positives = 59/129 (45%)
Frame = +2
Query: 137 YENKSLEYIFNHQTKLNK*NNGSRFDESVLYKSEFAFTREVIELTRIIDQSSSTKDLQTI 316
YEN EY +T LNK N E +L+ RE ++S T +Q+I
Sbjct: 218 YENDRDEYQATIET-LNKTNK--ELSEELLHYHSKLKERE--------EKSKVTSQMQSI 266
Query: 317 FPQLINNIFSSSFSTGWDLKSITCDVNRYEFEALISFLEPQGPMFRLCYRLLSDTQLKYE 496
P I+ FS + S D+KS T N F S ++ + F+ R+L+DTQL+YE
Sbjct: 267 -PMDIDRSFSQAISESSDIKSSTSASNHQSF----SMMKQE---FK---RILADTQLRYE 315
Query: 497 LPLNYYLLI 523
L L+
Sbjct: 316 KELEQERLL 324
>UniRef50_Q4UHH4 Cluster: Putative uncharacterized protein; n=3;
Theileria|Rep: Putative uncharacterized protein -
Theileria annulata
Length = 555
Score = 33.1 bits (72), Expect = 7.5
Identities = 20/69 (28%), Positives = 37/69 (53%), Gaps = 3/69 (4%)
Frame = +2
Query: 140 ENKSLEYIFNHQTKLNK*NNGSRFDESVLYK---SEFAFTREVIELTRIIDQSSSTKDLQ 310
ENK + F + + + N S +D+++LYK + + R + +LT ++ S +T
Sbjct: 339 ENKIIIKDFLYNILIERINEDSHYDKNILYKLKNKTYEYIRNIYQLT--VNTSYNTILTT 396
Query: 311 TIFPQLINN 337
TI+P +I N
Sbjct: 397 TIYPNIILN 405
>UniRef50_Q59TI0 Cluster: Hypothetical LPF family protein 31; n=2;
Candida albicans|Rep: Hypothetical LPF family protein 31
- Candida albicans (Yeast)
Length = 711
Score = 33.1 bits (72), Expect = 7.5
Identities = 25/75 (33%), Positives = 37/75 (49%), Gaps = 3/75 (4%)
Frame = +2
Query: 302 DLQTIFPQLINNIFSSSFSTGWDL--KSITCDVNRYEFEALISFLEPQGPMFRLCYRLLS 475
D I+P+ I F+ SF GWD+ K +T + Y F S P+ + LL
Sbjct: 94 DKWNIYPKTIKTEFTDSFEIGWDVFSKLLTKASSIYGFFVGTSNYPPEE-----LFELLM 148
Query: 476 DTQLKYE-LPLNYYL 517
D+ +K+E L L Y+L
Sbjct: 149 DSNVKFESLHLMYFL 163
>UniRef50_Q96A72 Cluster: Protein mago nashi homolog 2; n=89;
Eukaryota|Rep: Protein mago nashi homolog 2 - Homo
sapiens (Human)
Length = 148
Score = 33.1 bits (72), Expect = 7.5
Identities = 18/62 (29%), Positives = 32/62 (51%)
Frame = +2
Query: 137 YENKSLEYIFNHQTKLNK*NNGSRFDESVLYKSEFAFTREVIELTRIIDQSSSTKDLQTI 316
+ ++ LE+ F KL NN + ++ ++ K + + EL RIID S TK+ +
Sbjct: 19 FGHEFLEFEFRPDGKLRYANNSNYKNDVMIRKEAYVHKSVMEELKRIIDDSEITKEDDAL 78
Query: 317 FP 322
+P
Sbjct: 79 WP 80
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 665,470,684
Number of Sequences: 1657284
Number of extensions: 13045048
Number of successful extensions: 25328
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 24313
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25320
length of database: 575,637,011
effective HSP length: 99
effective length of database: 411,565,895
effective search space used: 61323318355
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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