BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30518.Seq (698 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac... 120 3e-28 SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein S15a... 29 0.85 SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein S... 29 0.85 SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schi... 28 1.1 SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac... 28 1.5 SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr... 27 2.6 SPCC1919.03c |||AMP-activated protein kinase beta subunit |Schiz... 26 6.0 SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyce... 26 6.0 SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr ... 26 6.0 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.0 SPBC1685.01 |pmp1||dual-specificity MAP kinase phosphatase Pmp1|... 25 7.9 >SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosaccharomyces pombe|chr 1|||Manual Length = 815 Score = 120 bits (288), Expect = 3e-28 Identities = 54/110 (49%), Positives = 76/110 (69%) Frame = +2 Query: 233 LAQGQTPQETVCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 412 + +G+ ++TV IVL+D+ D R+ D+V+I PCP +KY +R+ +L Sbjct: 78 VVKGKRRKDTVLIVLTDEEMEDGVARINRVVRNNLRVRLGDIVTINPCPDIKYAERISVL 137 Query: 413 PIDDSVEGLTGNLFEVYLKPYFMEAYRPIHRDTPFMVRGGMRAVEFKVVE 562 P+ D+VEGLTG+LF+VYLKPYF+EAYRPI + F+VRG MR VEFKVV+ Sbjct: 138 PLADTVEGLTGSLFDVYLKPYFVEAYRPIRKGDLFVVRGSMRQVEFKVVD 187 Score = 83.0 bits (196), Expect = 4e-17 Identities = 38/65 (58%), Positives = 50/65 (76%) Frame = +3 Query: 63 NKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLK 242 N D S +D +TAILR+K +PN L+V++A +DDNSV+ LS ME LQLFRGDTV++K Sbjct: 21 NPPKDTYSAEDTATAILRKKRKPNSLVVDDATNDDNSVITLSSNTMETLQLFRGDTVVVK 80 Query: 243 GKRRR 257 GKRR+ Sbjct: 81 GKRRK 85 >SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein S15a|Schizosaccharomyces pombe|chr 1|||Manual Length = 130 Score = 28.7 bits (61), Expect = 0.85 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%) Frame = -1 Query: 431 QLNHQLAVCGLV---FHISLKDTERWI 360 QLN ++ CG++ F++ LKD E+W+ Sbjct: 64 QLNGRINKCGVISPRFNVKLKDIEKWV 90 >SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein S15a|Schizosaccharomyces pombe|chr 1|||Manual Length = 130 Score = 28.7 bits (61), Expect = 0.85 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%) Frame = -1 Query: 431 QLNHQLAVCGLV---FHISLKDTERWI 360 QLN ++ CG++ F++ LKD E+W+ Sbjct: 64 QLNGRINKCGVISPRFNVKLKDIEKWV 90 >SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schizosaccharomyces pombe|chr 1|||Manual Length = 319 Score = 28.3 bits (60), Expect = 1.1 Identities = 12/39 (30%), Positives = 20/39 (51%), Gaps = 1/39 (2%) Frame = +1 Query: 88 LMIYRPRSSVARTDPTVSLSKKQSAMTTQSWH-FHRPKW 201 L + R + DP ++ + Q + T+SWH FH +W Sbjct: 259 LALIRGCDGLLSDDPVMARALSQGPIVTKSWHYFHYSEW 297 >SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 1|||Manual Length = 905 Score = 27.9 bits (59), Expect = 1.5 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%) Frame = -1 Query: 401 LVFHISLKDTERWIP-HQKDAHEGCFSQHGSSEFSHQG 291 LV + LKD WI + + E F G ++FS+QG Sbjct: 435 LVSDLYLKDLWSWIHLSHRQSEESLFGDTGDTDFSYQG 472 >SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 382 Score = 27.1 bits (57), Expect = 2.6 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 2/62 (3%) Frame = +1 Query: 37 FLDSNNKF*IKWQIIKALMIYRPRSSVARTDPTVS--LSKKQSAMTTQSWHFHRPKWSNF 210 F+++N K KW++ A+ P S + + PT+S + + SA TT +RP S Sbjct: 286 FIEANIK---KWRLAPAVGSLAPPPSYSTSQPTISWDAASESSAGTTTQGDTNRPDKSKN 342 Query: 211 NS 216 S Sbjct: 343 RS 344 >SPCC1919.03c |||AMP-activated protein kinase beta subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 298 Score = 25.8 bits (54), Expect = 6.0 Identities = 11/34 (32%), Positives = 17/34 (50%) Frame = +1 Query: 520 GPRGHARRRVQSGRKQIHHHFASWAPXTVIHCDG 621 G GH +RR Q+ K+ H ++ T+I G Sbjct: 74 GSSGHTKRRSQTSGKKTHQPYSGPCVPTIIRWRG 107 >SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyces pombe|chr 3|||Manual Length = 1008 Score = 25.8 bits (54), Expect = 6.0 Identities = 11/37 (29%), Positives = 21/37 (56%) Frame = +3 Query: 147 EEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKRRR 257 E+AV D+N+ ++L+ + + RGD ++ G R Sbjct: 247 EKAVCDENTKISLTNTEHYKFHSLRGDVEVVVGDLER 283 >SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 409 Score = 25.8 bits (54), Expect = 6.0 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 1/27 (3%) Frame = +3 Query: 87 PD-DLSTAILRRKDRPNRLIVEEAVSD 164 PD D + + + RK+ PNRL++ A+S+ Sbjct: 233 PDADANISKIARKEDPNRLVLASAISE 259 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 6.0 Identities = 14/53 (26%), Positives = 30/53 (56%) Frame = +3 Query: 60 LNKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLF 218 L +A N + L+T +RK + N ++ +A ++ N+ +++ A E+ QL+ Sbjct: 768 LGSLAGNLPQESLTTKS-KRKSKVNLELMFDATAEKNNENSMNSAVFEKSQLY 819 >SPBC1685.01 |pmp1||dual-specificity MAP kinase phosphatase Pmp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 278 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/40 (32%), Positives = 19/40 (47%) Frame = -2 Query: 604 RYXEPTMQNGDGSVFDHFELDGAHAPADHERCVTMDRTVS 485 R+ + N D VFDH E H D + + +D+ VS Sbjct: 102 RHYRDSKHNLDIQVFDHIEYVHIHWDHDTQFALELDKLVS 141 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,839,655 Number of Sequences: 5004 Number of extensions: 57174 Number of successful extensions: 168 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 162 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 168 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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