BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30509.Seq (548 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharo... 84 1e-17 SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizo... 59 4e-10 SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 27 2.4 SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 26 4.2 SPAC17G6.17 |pof8||F-box protein Pof8|Schizosaccharomyces pombe|... 26 4.2 SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr... 25 5.6 SPBC1685.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 25 9.7 SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyc... 25 9.7 >SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharomyces pombe|chr 2|||Manual Length = 302 Score = 83.8 bits (198), Expect = 1e-17 Identities = 37/87 (42%), Positives = 57/87 (65%), Gaps = 2/87 (2%) Frame = +1 Query: 1 ISVKVHPVVLFQIVDAYERRNADSHRVIGTLLGT--SDKGVVEVTNCFCVPHKEHADQVE 174 +++ + P VLF I+D R++ ++ RVIGTLLGT D +E+ +CF VPH E ++QVE Sbjct: 21 LNIVIEPAVLFSILDHSTRKSENNQRVIGTLLGTRSEDGREIEIKSCFAVPHNESSEQVE 80 Query: 175 AELNYAMDVYELNRRVNSSESIVGWWA 255 E+ Y +Y L+ + N E +VGW+A Sbjct: 81 VEMEYHRAMYHLHLKANPREVVVGWYA 107 >SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizosaccharomyces pombe|chr 3|||Manual Length = 324 Score = 59.3 bits (137), Expect = 4e-10 Identities = 29/86 (33%), Positives = 48/86 (55%), Gaps = 4/86 (4%) Frame = +1 Query: 7 VKVHPVVLFQIVDAYERR-NADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVEA-- 177 V VHP+VL VD+Y R RV+G LLG ++ VV V N + +P +E Sbjct: 17 VIVHPLVLLSAVDSYNRSAKGTKRRVVGILLGQNNGDVVNVANSYAIPFEEDEKNASVWF 76 Query: 178 -ELNYAMDVYELNRRVNSSESIVGWW 252 + N+ + E+ +++N++E +VGW+ Sbjct: 77 LDHNFMESMNEMFKKINANEKLVGWY 102 >SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1334 Score = 26.6 bits (56), Expect = 2.4 Identities = 13/42 (30%), Positives = 21/42 (50%) Frame = +1 Query: 49 YERRNADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVE 174 Y+ R DSHR I L GT + + + + +K H ++E Sbjct: 483 YDHRLRDSHREIYQLQGTGYRPFLRANDNASINNKNHHKELE 524 >SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc25|Schizosaccharomyces pombe|chr 1|||Manual Length = 596 Score = 25.8 bits (54), Expect = 4.2 Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 1/49 (2%) Frame = +3 Query: 60 KCRFSQSYRHLIGHERQRSGG-SNQLLLRATQRTCRSSRSGT*LRDGCL 203 + R S SY I ++ SGG + + L A RTC S + T L + CL Sbjct: 201 RSRSSSSYS--INKKKGTSGGQATRHLTYALSRTCSQSSNTTSLLESCL 247 >SPAC17G6.17 |pof8||F-box protein Pof8|Schizosaccharomyces pombe|chr 1|||Manual Length = 402 Score = 25.8 bits (54), Expect = 4.2 Identities = 18/71 (25%), Positives = 33/71 (46%), Gaps = 2/71 (2%) Frame = -1 Query: 518 TRDPTRHSLLAHL*TYNLRFIACESDI--YRSEHATLLSIWHSQWYTNICT*THSTSSQR 345 T T HSLL+++ + + IACE YR + + W + + C ++ +Q Sbjct: 304 TNKSTIHSLLSYVFSRQTQNIACEPMYIDYRKDETEAIIRWKTPLHAETC--INAFRTQE 361 Query: 344 SVQSNMDRLTA 312 Q++ D + A Sbjct: 362 RKQNSHDDIRA 372 >SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 131 Score = 25.4 bits (53), Expect = 5.6 Identities = 6/11 (54%), Positives = 10/11 (90%) Frame = +2 Query: 422 HVHSCRCHSHM 454 H+H CRCH+++ Sbjct: 69 HIHCCRCHTYI 79 >SPBC1685.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 325 Score = 24.6 bits (51), Expect = 9.7 Identities = 15/37 (40%), Positives = 22/37 (59%), Gaps = 2/37 (5%) Frame = -1 Query: 455 ACE--SDIYRSEHATLLSIWHSQWYTNICT*THSTSS 351 AC+ +D+YRS+ L S W SQ T+I + ST + Sbjct: 93 ACQENADLYRSQQRKLKSRWDSQ--TDILSQIESTKA 127 >SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 24.6 bits (51), Expect = 9.7 Identities = 19/59 (32%), Positives = 24/59 (40%) Frame = -2 Query: 409 PFGTPNGTQTYARKPIRPPASEVSRVTWTGSRXSRE*YSCITEEWLVTSLPVPTNQQYF 233 P G+ +Q A I S +T G S YS TEE L+T L T+ F Sbjct: 903 PTGSYEVSQPLANVTILGLTESPSSITLNGQNVSSFQYSNDTEELLITGLQNITSSGAF 961 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,514,999 Number of Sequences: 5004 Number of extensions: 54252 Number of successful extensions: 162 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 155 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 160 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 227943826 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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