BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30489.Seq (749 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC31A2.02 |trm112||tRNA |Schizosaccharomyces pombe|chr 1|||Manual 43 4e-05 SPBC1604.17c |||conserved fungal protein|Schizosaccharomyces pom... 31 0.23 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 30 0.40 SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|c... 26 6.6 SPAC7D4.03c |||conserved fungal family|Schizosaccharomyces pombe... 26 6.6 >SPAC31A2.02 |trm112||tRNA |Schizosaccharomyces pombe|chr 1|||Manual Length = 126 Score = 43.2 bits (97), Expect = 4e-05 Identities = 20/84 (23%), Positives = 40/84 (47%), Gaps = 1/84 (1%) Frame = +2 Query: 263 YPLAINATDIKVKEVDFNPEFISRVIPKLDWEVLWVAADSIGHSDGLPRSLENKYDENEE 442 +PL + + +++++ PEF+ ++P++DW L +G+ LP + D+++E Sbjct: 23 FPLDVVDAKLAIQQLELKPEFLIGIMPRIDWNALLKTTRQLGNY-SLPDEKPDLVDDSDE 81 Query: 443 -FLKKAHKXXXXXXXXXGHLTCPN 511 LK H G + C N Sbjct: 82 VLLKSLHNVLLETEITEGKMVCGN 105 Score = 31.5 bits (68), Expect = 0.13 Identities = 12/20 (60%), Positives = 17/20 (85%) Frame = +1 Query: 514 GRQFPISKGIPNMLLNEAEV 573 G +PI +GIPNMLL+E+E+ Sbjct: 107 GHVYPIFEGIPNMLLSESEI 126 >SPBC1604.17c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 459 Score = 30.7 bits (66), Expect = 0.23 Identities = 12/35 (34%), Positives = 21/35 (60%) Frame = +2 Query: 311 FNPEFISRVIPKLDWEVLWVAADSIGHSDGLPRSL 415 F +FI V+ +++WE+L + G +GL R+L Sbjct: 100 FQEQFIENVVKEINWEILAILISLSGIENGLVRNL 134 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 29.9 bits (64), Expect = 0.40 Identities = 25/70 (35%), Positives = 35/70 (50%), Gaps = 1/70 (1%) Frame = -3 Query: 492 PSSTSTSNKTL*AFFKNSSFSSYLFSKDLGRPSLWPMLSAATHNTSQS-SFGITRLINSG 316 PS +STS+ T + S+ SSYL S + S P S+++ TS S S +S Sbjct: 187 PSVSSTSSSTFSSAAPTSTSSSYLSSSSVVSSSSSPSSSSSSTLTSSSLSTSSIPSTSSS 246 Query: 315 LKSTSLTLMS 286 STS +L S Sbjct: 247 SSSTSSSLSS 256 >SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1111 Score = 25.8 bits (54), Expect = 6.6 Identities = 12/32 (37%), Positives = 15/32 (46%) Frame = +2 Query: 311 FNPEFISRVIPKLDWEVLWVAADSIGHSDGLP 406 F+PE + + LDW W A S G LP Sbjct: 577 FSPEVRNGFLKTLDWLSQWACARSYGLGTRLP 608 >SPAC7D4.03c |||conserved fungal family|Schizosaccharomyces pombe|chr 1|||Manual Length = 886 Score = 25.8 bits (54), Expect = 6.6 Identities = 18/65 (27%), Positives = 29/65 (44%), Gaps = 3/65 (4%) Frame = -1 Query: 515 PDLDKSSVPLQ---LPPLTRLYELSSKTLRFHHTYFLKILADHHCGRCYQRPPIILPNPA 345 P+LDK L + L RL S + R +T + +A HHC + ++ Sbjct: 192 PELDKLRHSLYNWWVSILRRLQSNISTSERITYTKAILAIAKHHCWNKVEHSALLYLEHQ 251 Query: 344 LVLHD 330 L+L+D Sbjct: 252 LILYD 256 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,906,200 Number of Sequences: 5004 Number of extensions: 58647 Number of successful extensions: 152 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 149 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 152 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 357280532 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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