Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30483.Seq
(667 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g77140.1 68414.m08986 vacuolar protein sorting protein 45, pu... 79 3e-15
At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putat... 37 0.014
At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE... 36 0.024
At3g15060.1 68416.m01905 Ras-related GTP-binding family protein ... 29 2.8
At3g53510.1 68416.m05908 ABC transporter family protein breast c... 29 3.7
At5g14390.1 68418.m01681 expressed protein 28 4.9
At3g01690.1 68416.m00101 expressed protein 28 4.9
At4g24760.1 68417.m03545 expressed protein 28 6.4
At4g31020.2 68417.m04406 expressed protein 27 8.5
At4g31020.1 68417.m04405 expressed protein 27 8.5
At3g11900.1 68416.m01459 amino acid transporter family protein l... 27 8.5
At2g24320.1 68415.m02907 hypothetical protein 27 8.5
>At1g77140.1 68414.m08986 vacuolar protein sorting protein 45,
putative / VPS45p, putative identical to vacuolar
protein sorting homolog VPS45p [Arabidopsis thaliana]
gi|2921406|gb|AAC39472
Length = 569
Score = 79.0 bits (186), Expect = 3e-15
Identities = 33/77 (42%), Positives = 54/77 (70%)
Frame = +3
Query: 255 HMKCIVFIRPTSENIALLSRELRDPKYGVYFIYFSNVVSKADIKTLAECDEXEAVREVQE 434
H+K + FIRPTS+NI L +L +P++G Y ++FSN++ I LA+ DE E V++VQE
Sbjct: 66 HLKAVYFIRPTSDNIQKLRYQLANPRFGEYHLFFSNLLKDTQIHILADSDEQEVVQQVQE 125
Query: 435 VFADYLAVDRHLFSFNI 485
+AD+++ D + F+ N+
Sbjct: 126 YYADFVSGDPYHFTLNM 142
Score = 60.9 bits (141), Expect = 7e-10
Identities = 31/57 (54%), Positives = 42/57 (73%)
Frame = +1
Query: 61 MNVIQAVKMYITKMTXXSGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDS 231
M ++ +V+ YI +M GMKV+++D ET S VS+VYSQSE+LQKEV+L E IDS
Sbjct: 1 MVLVTSVRDYINRMLQDIS-GMKVLILDSETVSNVSIVYSQSELLQKEVFLVEMIDS 56
>At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putative
similar to cytokinesis-related Sec1 protein KEULE
[Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
Pfam domain, PF00995: Sec1 family
Length = 662
Score = 36.7 bits (81), Expect = 0.014
Identities = 21/84 (25%), Positives = 45/84 (53%), Gaps = 3/84 (3%)
Frame = +3
Query: 258 MKCIVFIRPTSENIALLSREL--RDPKYGVYFIYFSNVVSKADIKTL-AECDEXEAVREV 428
M+ I FI+PT EN+ ++ + P Y F++FS+ VS++ + + + + + +
Sbjct: 86 MEVIYFIQPTEENVTAFLSDMTGKSPLYKKAFVFFSSPVSRSLVNLIKKDMRAMKRIGGL 145
Query: 429 QEVFADYLAVDRHLFSFNIVGCLQ 500
+E+ +Y+++D F N L+
Sbjct: 146 KEMNLEYISMDIQGFVTNNENALE 169
Score = 31.9 bits (69), Expect = 0.39
Identities = 16/36 (44%), Positives = 21/36 (58%)
Frame = +1
Query: 127 KVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSH 234
KV++MDK T I+S SEI Q+ + L E I H
Sbjct: 44 KVLVMDKFTVKIMSSACKMSEITQEGISLVEVITKH 79
>At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE)
similar to cytokinesis-related Sec1 protein KEULE
[Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
Pfam domain, PF00995: Sec1 family
Length = 666
Score = 35.9 bits (79), Expect = 0.024
Identities = 23/95 (24%), Positives = 45/95 (47%), Gaps = 3/95 (3%)
Frame = +3
Query: 258 MKCIVFIRPTSENIALLSREL--RDPKYGVYFIYFSNVVSKADIKTL-AECDEXEAVREV 428
M I FI+PT EN+ + ++ + P Y F++FS+ VSK + + + + +
Sbjct: 86 MDAIYFIQPTKENVIMFLSDMSGKSPLYKKAFVFFSSPVSKELVGHIKKDSSVLPRIGAL 145
Query: 429 QEVFADYLAVDRHLFSFNIVGCLQGRVGTNSISNE 533
+E+ ++ A+D F + L+ G S +
Sbjct: 146 REMNLEFFAIDSQGFITDHERALEDLFGDEETSRK 180
>At3g15060.1 68416.m01905 Ras-related GTP-binding family protein
similar to GTP-binding protein GI:303742 from [Pisum
sativum]; contains Pfam profile: PF00071 ras family
Length = 217
Score = 29.1 bits (62), Expect = 2.8
Identities = 20/55 (36%), Positives = 30/55 (54%), Gaps = 2/55 (3%)
Frame = -3
Query: 500 LEASN--NIEREQVSVHSQVVSKNFLDLSDSLXFITFGQSFDVCLRNYITEIYKV 342
LEA N N E +S +V SK LD+ D + GQS +V ++ ++E+ KV
Sbjct: 158 LEALNVENAFTEVLSQIYRVASKKALDIGDDHTTLPKGQSINVGSKDDVSEVKKV 212
>At3g53510.1 68416.m05908 ABC transporter family protein breast
cancer resistance protein (BCRP), Homo sapiens,
EMBL:AF098951
Length = 739
Score = 28.7 bits (61), Expect = 3.7
Identities = 15/49 (30%), Positives = 27/49 (55%)
Frame = -3
Query: 176 YTILTMLVVSLSINITFIPGPLSSVILVMYILTACITFILIKLSLFISQ 30
+TILT S +TF+ G +S V++ ++ A + + L+ FIS+
Sbjct: 568 FTILTAFWAGSSF-VTFLSGVVSHVMIGFTVVVAILAYFLLFSGFFISR 615
>At5g14390.1 68418.m01681 expressed protein
Length = 369
Score = 28.3 bits (60), Expect = 4.9
Identities = 16/43 (37%), Positives = 26/43 (60%), Gaps = 1/43 (2%)
Frame = -3
Query: 566 ETGGAPADLEKLVGDAVGSNPTLEASNNIEREQVSV-HSQVVS 441
ET GA + L G +VGS PTL+ + + + + +V HS ++S
Sbjct: 132 ETYGAKQEDIILYGQSVGSGPTLDLAARLPQLRAAVLHSPILS 174
>At3g01690.1 68416.m00101 expressed protein
Length = 361
Score = 28.3 bits (60), Expect = 4.9
Identities = 15/43 (34%), Positives = 26/43 (60%), Gaps = 1/43 (2%)
Frame = -3
Query: 566 ETGGAPADLEKLVGDAVGSNPTLEASNNI-EREQVSVHSQVVS 441
ET G+ + L G +VGS PTL+ ++ + + V +HS ++S
Sbjct: 132 ETFGSKQEGVILYGQSVGSGPTLDLASRLPQLRAVVLHSPILS 174
>At4g24760.1 68417.m03545 expressed protein
Length = 365
Score = 27.9 bits (59), Expect = 6.4
Identities = 15/43 (34%), Positives = 25/43 (58%), Gaps = 1/43 (2%)
Frame = -3
Query: 566 ETGGAPADLEKLVGDAVGSNPTLEASNNIEREQVSV-HSQVVS 441
E GA + L G +VGS PT++ + + R + S+ HS ++S
Sbjct: 132 ENYGAKQENIILYGQSVGSGPTVDLAARLPRLRASILHSPILS 174
>At4g31020.2 68417.m04406 expressed protein
Length = 294
Score = 27.5 bits (58), Expect = 8.5
Identities = 13/32 (40%), Positives = 21/32 (65%), Gaps = 1/32 (3%)
Frame = -3
Query: 533 LVGDAVGSNPTLEASNNIER-EQVSVHSQVVS 441
L G +VGS PTL ++ ++R V +HS ++S
Sbjct: 143 LYGQSVGSGPTLHMASRLKRLRGVVLHSAILS 174
>At4g31020.1 68417.m04405 expressed protein
Length = 294
Score = 27.5 bits (58), Expect = 8.5
Identities = 13/32 (40%), Positives = 21/32 (65%), Gaps = 1/32 (3%)
Frame = -3
Query: 533 LVGDAVGSNPTLEASNNIER-EQVSVHSQVVS 441
L G +VGS PTL ++ ++R V +HS ++S
Sbjct: 143 LYGQSVGSGPTLHMASRLKRLRGVVLHSAILS 174
>At3g11900.1 68416.m01459 amino acid transporter family protein low
similarity to proton/amino acid transporter 1 [Mus
musculus] GI:21908024; belongs to INTERPRO:IPR002422
amino acid/polyamine transporter, family II
Length = 432
Score = 27.5 bits (58), Expect = 8.5
Identities = 15/41 (36%), Positives = 24/41 (58%)
Frame = -3
Query: 179 LYTILTMLVVSLSINITFIPGPLSSVILVMYILTACITFIL 57
++T T+LVV L+ + +PG + LV L A I+F+L
Sbjct: 349 IFTTRTLLVVGLAAIASLVPGFGTFASLVGSTLCALISFVL 389
>At2g24320.1 68415.m02907 hypothetical protein
Length = 286
Score = 27.5 bits (58), Expect = 8.5
Identities = 12/32 (37%), Positives = 21/32 (65%), Gaps = 1/32 (3%)
Frame = -3
Query: 533 LVGDAVGSNPTLEASNNIER-EQVSVHSQVVS 441
L G +VGS PTL ++ ++R + +HS ++S
Sbjct: 135 LYGQSVGSGPTLHLASRVKRLRGIVLHSAILS 166
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,184,557
Number of Sequences: 28952
Number of extensions: 246417
Number of successful extensions: 597
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 587
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 596
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1403159472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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