Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30477.Seq
         (696 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccha...   108   6e-25
SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosacchar...   107   1e-24
SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|...    29   0.48 
SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki...    27   3.4  
SPBC660.14 |mik1||mitotic inhibitor kinase Mik1|Schizosaccharomy...    26   5.9  
SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc...    26   5.9  
SPCC1322.09 |||conserved fungal protein|Schizosaccharomyces pomb...    25   7.8  

>SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein
           S8|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 200

 Score =  108 bits (260), Expect = 6e-25
 Identities = 76/203 (37%), Positives = 109/203 (53%), Gaps = 2/203 (0%)
 Frame = +1

Query: 31  MGISRDHWHKRRATGWETCAHTQEEEV*VRASRCKHQARPSA-NPLRSFTW-WKY*VPCA 204
           MGI+RD  HKR ATG +   + ++ +  +       +  P   + +R      K+     
Sbjct: 1   MGITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRL 60

Query: 205 ASGHR*LLLGIGMFNSQTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYT 384
            SG+     G    + +TRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY 
Sbjct: 61  DSGN--FSWGSEGVSKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYG 118

Query: 385 LPLGRKKGAKLTEAEEAIINKKRSQKTARKYLARQRLAKVEVL*KSNSTQGVCWACVASR 564
           + +G  KG K T          +S+   RK+ AR   +KV+   ++    G  +A V+SR
Sbjct: 119 ILMG-SKGKKATSTP-----NPKSKHVQRKHSARLGDSKVDSALETQFAAGRLYAVVSSR 172

Query: 565 PGQCGRADGLHLRXAKKLEFYLR 633
           PGQ GR DG ++   ++L FYLR
Sbjct: 173 PGQSGRCDG-YILEGEELHFYLR 194



 Score = 94.7 bits (225), Expect = 1e-20
 Identities = 42/59 (71%), Positives = 50/59 (84%)
 Frame = +2

Query: 80  KRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLDTGNFSWGSECSTRK 256
           KRA  RKKRK+ELGR  +NTR+GP+RIH VR RGGN K+RALRLD+GNFSWGSE  ++K
Sbjct: 17  KRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRLDSGNFSWGSEGVSKK 75


>SPAC521.05 |rps802|rps8-2|40S ribosomal protein
           S8|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 200

 Score =  107 bits (258), Expect = 1e-24
 Identities = 76/203 (37%), Positives = 109/203 (53%), Gaps = 2/203 (0%)
 Frame = +1

Query: 31  MGISRDHWHKRRATGWETCAHTQEEEV*VRASRCKHQARPSA-NPLRSFTW-WKY*VPCA 204
           MGI+RD  HKR ATG +   + ++ +  +       +  P   + +R      K+     
Sbjct: 1   MGITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRL 60

Query: 205 ASGHR*LLLGIGMFNSQTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYT 384
            SG+     G    + +TRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY 
Sbjct: 61  DSGN--FSWGSEGVSKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYG 118

Query: 385 LPLGRKKGAKLTEAEEAIINKKRSQKTARKYLARQRLAKVEVL*KSNSTQGVCWACVASR 564
           + +G  KG K T          +S+   RK+ AR   +KV+   ++    G  +A V+SR
Sbjct: 119 ILMG-SKGKKATATP-----TPKSKHVQRKHSARLGDSKVDSALETQFAAGRLYAVVSSR 172

Query: 565 PGQCGRADGLHLRXAKKLEFYLR 633
           PGQ GR DG ++   ++L FYLR
Sbjct: 173 PGQSGRCDG-YILEGEELHFYLR 194



 Score = 94.7 bits (225), Expect = 1e-20
 Identities = 42/59 (71%), Positives = 50/59 (84%)
 Frame = +2

Query: 80  KRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLDTGNFSWGSECSTRK 256
           KRA  RKKRK+ELGR  +NTR+GP+RIH VR RGGN K+RALRLD+GNFSWGSE  ++K
Sbjct: 17  KRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRLDSGNFSWGSEGVSKK 75


>SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 207

 Score = 29.5 bits (63), Expect = 0.48
 Identities = 16/50 (32%), Positives = 25/50 (50%)
 Frame = +2

Query: 59  SEGPPAGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALR 208
           S+G    K+    +KRK  L R A   RL  + + S+ S+GG    +A +
Sbjct: 35  SKGVKTNKKEQEAEKRKAALERKAERERLEKEEMESLPSKGGKGSKKAAK 84


>SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase
            Win1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1436

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 16/49 (32%), Positives = 23/49 (46%)
 Frame = +2

Query: 113  ELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLDTGNFSWGSECSTRKP 259
            EL    +N  +  Q+   + S    T YRA+ LDTG+     E +  KP
Sbjct: 1108 ELASSKSNITIRWQQGGLIGSGSFGTVYRAVNLDTGDLMAVKEVALHKP 1156


>SPBC660.14 |mik1||mitotic inhibitor kinase Mik1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 581

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 21/64 (32%), Positives = 29/64 (45%), Gaps = 1/64 (1%)
 Frame = -2

Query: 674 RLHIITSPS*NLTXLR*NSSFFAXLRCKP-SARPH*PGRLATQAQQTPCVELLF*STSTL 498
           R   +TS S  L   R   S +      P S +PH P +  T+ +  P    L  +TS L
Sbjct: 124 RKRAVTSLSTGLLSKREKWSLWEGNLTNPRSEQPHTPCKKGTKIKLKPPQSPLSPTTSLL 183

Query: 497 ARRC 486
           AR+C
Sbjct: 184 ARKC 187


>SPBC16H5.10c |prp43||ATP-dependent RNA helicase
           Prp43|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 735

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 11/25 (44%), Positives = 15/25 (60%)
 Frame = -2

Query: 368 YHCLNGVASTTTIAFLTRVFVRTNS 294
           ++C N V S T +  +  VFVR NS
Sbjct: 518 FYCSNEVLSLTALLSVPNVFVRPNS 542


>SPCC1322.09 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 455

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 27/92 (29%), Positives = 40/92 (43%), Gaps = 11/92 (11%)
 Frame = -1

Query: 285 CIIHNINDTGLRVEHSDPQEKLPVS-RRSA---RYL-------VFPPRERTEWIR*GPSL 139
           C + ++NDT   +E S   + LP+  +R A   RY+       + P    TEW+    S+
Sbjct: 348 CFLIHLNDTNHNLEFSTTSQPLPLEWQRWAVDPRYIKLFGSNAILPNEWVTEWVE--QSI 405

Query: 138 VFAAGRPNSYFLFLRMGARFPAGGPSLMPVIP 43
             AAG     +   RM      G PSL   +P
Sbjct: 406 SVAAGIVAQMYTSKRMA----LGDPSLFAGLP 433


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,693,585
Number of Sequences: 5004
Number of extensions: 53429
Number of successful extensions: 148
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 146
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 321151040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)