Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30477.Seq
(696 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g20290.1 68418.m02415 40S ribosomal protein S8 (RPS8A) riboso... 133 9e-32
At5g59240.1 68418.m07424 40S ribosomal protein S8 (RPS8B) 40S ri... 127 6e-30
At2g22830.1 68415.m02711 squalene monooxygenase, putative / squa... 31 0.73
At5g44010.1 68418.m05386 expressed protein 29 2.2
At1g73260.1 68414.m08478 trypsin and protease inhibitor family p... 29 2.9
At5g44890.1 68418.m05504 Ulp1 protease family protein contains P... 29 3.9
At3g26530.1 68416.m03312 Ulp1 protease family protein contains P... 29 3.9
At1g21020.1 68414.m02630 hypothetical protein similar to At3g265... 29 3.9
At1g08740.1 68414.m00971 Ulp1 protease family protein contains P... 29 3.9
At4g04400.1 68417.m00634 hypothetical protein contains Pfam prof... 28 6.8
At5g57240.1 68418.m07151 oxysterol-binding family protein low si... 27 9.0
>At5g20290.1 68418.m02415 40S ribosomal protein S8 (RPS8A) ribosomal
protein S8 - Zea mays, PIR:T04088
Length = 222
Score = 133 bits (322), Expect = 9e-32
Identities = 98/228 (42%), Positives = 127/228 (55%), Gaps = 19/228 (8%)
Frame = +1
Query: 31 MGISRDHWHKRRATGWETCAHTQEEEV*VRASRCKHQARPSANPLRSF--TWWKY*VPCA 204
MGISRD HKRRATG + Q++ R R R AN S T + V
Sbjct: 1 MGISRDSIHKRRATGGK-----QKQW---RKKRKYEMGRQPANTKLSSNKTVRRIRVRGG 52
Query: 205 ASGHR*LLLGIGMFN-------SQTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQ 363
R L L G ++ +TR++DVVYNASNNELVRTKTLVK+AIV VDA PF+Q
Sbjct: 53 NVKWRALRLDTGNYSWGSEATTRKTRVLDVVYNASNNELVRTKTLVKSAIVQVDAAPFKQ 112
Query: 364 WYESHYTLPLGR-KKGAKLT-----EAEEAII----NKKRSQKTARKYLARQRLAKVEVL 513
WY SHY + LGR KK A T E EEA + K+S RK +RQ ++
Sbjct: 113 WYLSHYGVELGRKKKSASSTKKDGEEGEEAAVAAPEEVKKSNHLLRKIASRQEGRSLDSH 172
Query: 514 *KSNSTQGVCWACVASRPGQCGRADGLHLRXAKKLEFYLRXVKF*EGE 657
+ G AC++SRPGQCGRADG ++ K+LEFY++ ++ +G+
Sbjct: 173 IEDQFASGRLLACISSRPGQCGRADG-YILEGKELEFYMKKIQKKKGK 219
Score = 83.8 bits (198), Expect = 1e-16
Identities = 38/61 (62%), Positives = 47/61 (77%), Gaps = 1/61 (1%)
Frame = +2
Query: 77 GKRAPIRKKRKYELGRPAANTRLGPQR-IHSVRSRGGNTKYRALRLDTGNFSWGSECSTR 253
GK+ RKKRKYE+GR ANT+L + + +R RGGN K+RALRLDTGN+SWGSE +TR
Sbjct: 16 GKQKQWRKKRKYEMGRQPANTKLSSNKTVRRIRVRGGNVKWRALRLDTGNYSWGSEATTR 75
Query: 254 K 256
K
Sbjct: 76 K 76
>At5g59240.1 68418.m07424 40S ribosomal protein S8 (RPS8B) 40S
ribosomal protein S8, Prunus armeniaca, EMBL:AF071889
Length = 210
Score = 127 bits (307), Expect = 6e-30
Identities = 88/216 (40%), Positives = 119/216 (55%), Gaps = 7/216 (3%)
Frame = +1
Query: 31 MGISRDHWHKRRATGWETCAHTQEEEV*VRASRCKHQARPSANPLRSFTWWKY*VPCAAS 210
MGISRD HKRRATG + ++ + R + S+N T + V
Sbjct: 1 MGISRDSIHKRRATGGKQKMWRKKRKY--ELGRQPANTKLSSNK----TVRRIRVRGGNV 54
Query: 211 GHR*LLLGIGMFN-------SQTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWY 369
R L L G F+ +TRI+DV YNASNNELVRT+TLVK+AIV VDA PF+Q Y
Sbjct: 55 KWRALRLDTGNFSWGSEAVTRKTRILDVAYNASNNELVRTQTLVKSAIVQVDAAPFKQGY 114
Query: 370 ESHYTLPLGRKKGAKLTEAEEAIINKKRSQKTARKYLARQRLAKVEVL*KSNSTQGVCWA 549
HY + +GRKK + EE K+S RK RQ ++ + + G A
Sbjct: 115 LQHYGVDIGRKKKGEAVTTEEV----KKSNHVQRKLEMRQEGRALDSHLEEQFSSGRLLA 170
Query: 550 CVASRPGQCGRADGLHLRXAKKLEFYLRXVKF*EGE 657
C+ASRPGQCGRADG ++ K+LEFY++ ++ +G+
Sbjct: 171 CIASRPGQCGRADG-YILEGKELEFYMKKLQKKKGK 205
Score = 84.2 bits (199), Expect = 7e-17
Identities = 40/61 (65%), Positives = 46/61 (75%), Gaps = 1/61 (1%)
Frame = +2
Query: 77 GKRAPIRKKRKYELGRPAANTRLGPQR-IHSVRSRGGNTKYRALRLDTGNFSWGSECSTR 253
GK+ RKKRKYELGR ANT+L + + +R RGGN K+RALRLDTGNFSWGSE TR
Sbjct: 16 GKQKMWRKKRKYELGRQPANTKLSSNKTVRRIRVRGGNVKWRALRLDTGNFSWGSEAVTR 75
Query: 254 K 256
K
Sbjct: 76 K 76
>At2g22830.1 68415.m02711 squalene monooxygenase, putative /
squalene epoxidase, putative similar to SP|O65404 (SE
1,1), SP|O65402 (SE 1,2), SP|O65403 (SE 2)
Length = 585
Score = 31.1 bits (67), Expect = 0.73
Identities = 16/49 (32%), Positives = 25/49 (51%)
Frame = -2
Query: 377 CDSYHCLNGVASTTTIAFLTRVFVRTNSLLDALYTTSMIRVCELNIPIP 231
C Y L GV S+ +A L+ + R SL+ + ++ VC L +P P
Sbjct: 492 CFDYLSLGGVFSSGPVALLSGLNPRPLSLVLHFFAVAIYAVCRLMLPFP 540
>At5g44010.1 68418.m05386 expressed protein
Length = 357
Score = 29.5 bits (63), Expect = 2.2
Identities = 16/48 (33%), Positives = 27/48 (56%), Gaps = 2/48 (4%)
Frame = -1
Query: 327 ILDKGLCTHQFIVRCIIHNINDTGLRV--EHSDPQEKLPVSRRSARYL 190
I+D LC Q +V C+ + +TGL++ +H +P+E L A +L
Sbjct: 98 IIDGKLCKRQSVVSCV--SALETGLKILSKHIEPKESLHREDNDATFL 143
>At1g73260.1 68414.m08478 trypsin and protease inhibitor family
protein / Kunitz family protein similar to trypsin
inhibitor propeptide [Brassica oleracea] GI:841208;
contains Pfam profile PF00197: Trypsin and protease
inhibitor
Length = 215
Score = 29.1 bits (62), Expect = 2.9
Identities = 18/44 (40%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
Frame = +1
Query: 298 LVRTKTLVKNAI-VVVDATPFRQWYESHYTLPLGRKKGAKLTEA 426
L T L NA VVD ++ES+Y LP+ R +G LT A
Sbjct: 16 LALTAVLASNAYGAVVDIDGNAMFHESYYVLPVIRGRGGGLTLA 59
>At5g44890.1 68418.m05504 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain; similar to At1g21030, At2g29240,
At1g08740, At1g08760
Length = 1015
Score = 28.7 bits (61), Expect = 3.9
Identities = 21/77 (27%), Positives = 33/77 (42%), Gaps = 1/77 (1%)
Frame = -1
Query: 270 INDTGLRVEHSDPQEKLPVSRRSARYLVFPPRERTEWIR*GPSLVFAAGRPNSYFLFLRM 91
I GL + P + +P + + L+ PP + E+ PSL P+ Y + + M
Sbjct: 612 IGGEGLDDIQTSPDKPIPAAASHTQCLLPPPTDVNEFFESEPSL------PDPYVVLVDM 665
Query: 90 -GARFPAGGPSLMPVIP 43
G + G P L IP
Sbjct: 666 DGVAYNVGQPELQYTIP 682
>At3g26530.1 68416.m03312 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain; similar to At1g21020, At1g08760,
At1g08740, At2g29240, At1g35650
Length = 1015
Score = 28.7 bits (61), Expect = 3.9
Identities = 21/77 (27%), Positives = 33/77 (42%), Gaps = 1/77 (1%)
Frame = -1
Query: 270 INDTGLRVEHSDPQEKLPVSRRSARYLVFPPRERTEWIR*GPSLVFAAGRPNSYFLFLRM 91
I GL + P + +P + + L+ PP + E+ PSL P+ Y + + M
Sbjct: 612 IGGEGLDDIQTSPDKPIPAAASHTQCLLPPPTDVNEFFESEPSL------PDPYVVLVDM 665
Query: 90 -GARFPAGGPSLMPVIP 43
G + G P L IP
Sbjct: 666 DGVAYNVGQPELQYTIP 682
>At1g21020.1 68414.m02630 hypothetical protein similar to At3g26530,
At1g08760, At1g08740, At2g29240, At1g35650
Length = 751
Score = 28.7 bits (61), Expect = 3.9
Identities = 21/77 (27%), Positives = 33/77 (42%), Gaps = 1/77 (1%)
Frame = -1
Query: 270 INDTGLRVEHSDPQEKLPVSRRSARYLVFPPRERTEWIR*GPSLVFAAGRPNSYFLFLRM 91
I GL + P + +P + + L+ PP + E+ PSL P+ Y + + M
Sbjct: 612 IGGEGLDDIQTSPDKPIPAAASHTQCLLPPPTDVNEFFESEPSL------PDPYVVLVDM 665
Query: 90 -GARFPAGGPSLMPVIP 43
G + G P L IP
Sbjct: 666 DGVAYNVGQPELQYTIP 682
>At1g08740.1 68414.m00971 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain; similar to At1g21020, At3g26530,
At1g08760, At2g29240, At1g35650, At1g21030
Length = 1014
Score = 28.7 bits (61), Expect = 3.9
Identities = 21/77 (27%), Positives = 33/77 (42%), Gaps = 1/77 (1%)
Frame = -1
Query: 270 INDTGLRVEHSDPQEKLPVSRRSARYLVFPPRERTEWIR*GPSLVFAAGRPNSYFLFLRM 91
I GL + P + +P + + L+ PP + E+ PSL P+ Y + + M
Sbjct: 611 IGGEGLDDIQTSPDKPIPAAASHTQCLLPPPTDVNEFFESEPSL------PDPYVVLVDM 664
Query: 90 -GARFPAGGPSLMPVIP 43
G + G P L IP
Sbjct: 665 DGVAYNVGQPELQYTIP 681
>At4g04400.1 68417.m00634 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 735
Score = 27.9 bits (59), Expect = 6.8
Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
Frame = -1
Query: 333 NCILDKGLCTHQFIVRCIIHNINDTGL-RVEHSDPQEKLP 217
N I KG+C RCI++ I+D + +E+ P+ +P
Sbjct: 530 NMIWWKGMCEMDISSRCIVNEISDEEVHEIENEVPETAVP 569
>At5g57240.1 68418.m07151 oxysterol-binding family protein low
similarity to SP|P22059 Oxysterol-binding protein 1
{Homo sapiens}; contains Pfam profile PF01237:
Oxysterol-binding protein
Length = 379
Score = 27.5 bits (58), Expect = 9.0
Identities = 28/108 (25%), Positives = 49/108 (45%), Gaps = 1/108 (0%)
Frame = +1
Query: 247 NSQTRIIDVVYNASNNELVRTKT-LVKNAIVVVDATPFRQWYESHYTLPLGRKKGAKLTE 423
N++T ++V+Y AS N + + KT +V+N V ++ W E + + A+ E
Sbjct: 268 NTKTGQLEVIYKASEN-IAKLKTPIVENLQEVSESESAVVWGEVSEGIVTNNWEKAR--E 324
Query: 424 AEEAIINKKRSQKTARKYLARQRLAKVEVL*KSNSTQGVCWACVASRP 567
A+ + K+R RK + + K S + G W CV +P
Sbjct: 325 AKRDVEEKQRESLRKRKASGQSWIPKH----FSVARTGKDWDCVPLQP 368
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,403,513
Number of Sequences: 28952
Number of extensions: 295706
Number of successful extensions: 842
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 818
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 837
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1487069504
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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