BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30460.Seq (755 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 33 0.058 SPAC20G4.05c |||UPF0061 family protein|Schizosaccharomyces pombe... 27 2.9 SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch... 27 2.9 SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po... 27 2.9 SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces... 27 3.8 SPBC28F2.09 |||transcription factor TFIIA complex large subunit ... 26 5.0 SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2... 26 5.0 SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|c... 26 6.7 SPBC119.10 |asn1||asparagine synthetase|Schizosaccharomyces pomb... 26 6.7 >SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 409 Score = 32.7 bits (71), Expect = 0.058 Identities = 26/123 (21%), Positives = 55/123 (44%), Gaps = 3/123 (2%) Frame = +3 Query: 252 SRVWQWASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFL 431 ++V Q+ S+++SEL A + P + +++Q + A++ + D L ++T+L Sbjct: 91 AKVLQYCSFTNSELPGAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDEELASKTYL 150 Query: 432 VTERITCR---CHCLQYTAACFPARARPDVRSSLINVQRWFLTVAHQPQVSAVVGSLTLC 602 V R+T C A + + ++ R++ T+ HQ ++ A+ L Sbjct: 151 VGSRLTLADIFFTCFLKFGATYV--LTKSYLAKYTHIYRYYQTIYHQAKLDAITEPLKFI 208 Query: 603 AAP 611 P Sbjct: 209 DQP 211 Score = 31.9 bits (69), Expect = 0.10 Identities = 17/39 (43%), Positives = 23/39 (58%) Frame = +1 Query: 103 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN 219 N D KFP K+P F DG L+E+ AIA+Y+A+ Sbjct: 38 NFPADLAAKFPLQKMPVFVGKDG-FPLSETLAIAFYLAS 75 >SPAC20G4.05c |||UPF0061 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 568 Score = 27.1 bits (57), Expect = 2.9 Identities = 24/65 (36%), Positives = 32/65 (49%), Gaps = 1/65 (1%) Frame = +1 Query: 97 ETNKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVANESLRG-GDLATQAVSGSGH 273 ET K + K AG+ P ADGK +L S I Y+ E+L G TQA++ S Sbjct: 134 ETGKRFEIQVK-GAGRTPYSRFADGKAVLRSS--IREYLCCEALYALGIPTTQALAISNL 190 Query: 274 HGLTA 288 G+ A Sbjct: 191 EGVVA 195 >SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Schizosaccharomyces pombe|chr 1|||Manual Length = 738 Score = 27.1 bits (57), Expect = 2.9 Identities = 16/62 (25%), Positives = 30/62 (48%) Frame = +1 Query: 10 PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 189 P F++ KA + QY+ +FG +K ++F K+ ++ F S + +L+ Sbjct: 553 PNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEKLESEIVRF-SFEHSILINT 611 Query: 190 SN 195 SN Sbjct: 612 SN 613 >SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 220 Score = 27.1 bits (57), Expect = 2.9 Identities = 16/34 (47%), Positives = 18/34 (52%) Frame = +1 Query: 109 SEDFLKKFPAGKVPAFESADGKVLLTESNAIAYY 210 S D KFP K+P F ADG L+E AI Y Sbjct: 40 SADLAAKFPLQKLPVFIGADG-FELSEVIAIVKY 72 Score = 27.1 bits (57), Expect = 2.9 Identities = 18/88 (20%), Positives = 42/88 (47%), Gaps = 1/88 (1%) Frame = +3 Query: 312 WVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERIT-CRCHCLQYTAACF 488 WV + G + + ++ + + + + +LK+ + + RT+LV +R T F Sbjct: 116 WVGMFRGNIPYEEKPFKESATRAIDSLKIPNELVKDRTYLVGDRFTLADLFFGSLLRIFF 175 Query: 489 PARARPDVRSSLINVQRWFLTVAHQPQV 572 + R L ++ R+++T+ HQ ++ Sbjct: 176 NSIIDEKTRKELPHLTRYYITMFHQAKL 203 >SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 442 Score = 26.6 bits (56), Expect = 3.8 Identities = 11/27 (40%), Positives = 15/27 (55%) Frame = +1 Query: 376 TYWPP*KYWTDIFSHAPSLLPRESLAD 456 TY PP Y D+FS APS+ + + Sbjct: 257 TYLPPFNYDHDVFSFAPSVASADQFTE 283 >SPBC28F2.09 |||transcription factor TFIIA complex large subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 369 Score = 26.2 bits (55), Expect = 5.0 Identities = 12/25 (48%), Positives = 14/25 (56%) Frame = -1 Query: 179 STFPSALSNAGTFPAGNFFKKSSDL 105 +TFP A + GTFP G F S L Sbjct: 52 ATFPWAQAPVGTFPIGQLFDPVSGL 76 >SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2|Schizosaccharomyces pombe|chr 1|||Manual Length = 750 Score = 26.2 bits (55), Expect = 5.0 Identities = 13/33 (39%), Positives = 19/33 (57%) Frame = +1 Query: 406 DIFSHAPSLLPRESLADVIVFSTLLHAFQHVLD 504 DIFSHA L +L ++ ++TLL F + D Sbjct: 188 DIFSHASRYLFVTNLPRIVPYATLLELFSKLGD 220 >SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 339 Score = 25.8 bits (54), Expect = 6.7 Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 3/39 (7%) Frame = +1 Query: 268 GHHGLTANYCLLPALGSSLTLV--SCNSTN-RMLNVQSL 375 GH L + C P++GSS L+ +C N R+ V++L Sbjct: 213 GHTDLIRDICWAPSMGSSYYLIATACKDGNVRIFKVETL 251 >SPBC119.10 |asn1||asparagine synthetase|Schizosaccharomyces pombe|chr 2|||Manual Length = 557 Score = 25.8 bits (54), Expect = 6.7 Identities = 17/66 (25%), Positives = 29/66 (43%) Frame = +1 Query: 298 LLPALGSSLTLVSCNSTNRMLNVQSLTYWPP*KYWTDIFSHAPSLLPRESLADVIVFSTL 477 L+ ++ + T NST++ +++T WP + +P LL +AD Sbjct: 244 LIASIAARETEKLANSTSQSEEARTITAWPKLHSFAIGLPGSPDLLAARKVAD------F 297 Query: 478 LHAFQH 495 LH F H Sbjct: 298 LHTFHH 303 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,069,168 Number of Sequences: 5004 Number of extensions: 63470 Number of successful extensions: 196 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 193 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 196 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 361294920 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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