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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30456.Seq
         (478 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3H5.10 |rpl3202|rpl32-2, rpl32|60S ribosomal protein L32|Sch...   108   4e-25
SPBC16C6.11 |rpl3201|rpl32-1|60S ribosomal protein L32|Schizosac...   107   1e-24
SPBC6B1.10 |prp17||splicing factor Prp17|Schizosaccharomyces pom...    27   1.1  
SPAC139.03 |||transcription factor, zf-fungal binuclear cluster ...    27   1.5  
SPAC1250.04c |atl1||alkyltransferase-like protein Atl1|Schizosac...    27   1.5  
SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar...    25   4.5  
SPBC336.10c |tif512||translation initiation factor|Schizosacchar...    25   5.9  
SPBC21C3.20c |git1||C2 domain protein Git1|Schizosaccharomyces p...    25   5.9  
SPAC26H5.10c |tif51||translation initiation factor eIF5A|Schizos...    25   5.9  
SPAC15A10.15 |sgo2||shugoshin Sgo2|Schizosaccharomyces pombe|chr...    25   7.9  

>SPAC3H5.10 |rpl3202|rpl32-2, rpl32|60S ribosomal protein
           L32|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 127

 Score =  108 bits (260), Expect = 4e-25
 Identities = 61/124 (49%), Positives = 82/124 (66%), Gaps = 2/124 (1%)
 Frame = +2

Query: 65  IVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYGSNKKTRHMLP 244
           I+KKRTK F RHQSDR+ ++  +WRKPRGID+ VRRRF+G   MP IGYG+NKKTR+ +P
Sbjct: 6   IIKKRTKPFKRHQSDRFKRVGESWRKPRGIDSCVRRRFRGTISMPKIGYGNNKKTRYCMP 65

Query: 245 NGFLRS*FTMLKSWKS**CKTGST--AQRSLMVSLRRSGS*FVERAQQLSIRVTNAAARL 418
           NG L++      S          T  A+ +  VS R+     VE+A+ L ++VTNA A++
Sbjct: 66  NG-LKAFLVRNVSDVELLLMHNKTYAAEIAGNVSARKRVE-IVEKARALGVKVTNAGAKV 123

Query: 419 RSQE 430
           RSQE
Sbjct: 124 RSQE 127



 Score = 42.3 bits (95), Expect = 4e-05
 Identities = 21/47 (44%), Positives = 30/47 (63%)
 Frame = +1

Query: 241 PKWIPKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIRGKSPA 381
           P  +   LV NV ++E+L+M N+ Y AEIA  VS++KR  I  K+ A
Sbjct: 65  PNGLKAFLVRNVSDVELLLMHNKTYAAEIAGNVSARKRVEIVEKARA 111


>SPBC16C6.11 |rpl3201|rpl32-1|60S ribosomal protein
           L32|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 127

 Score =  107 bits (256), Expect = 1e-24
 Identities = 61/124 (49%), Positives = 81/124 (65%), Gaps = 2/124 (1%)
 Frame = +2

Query: 65  IVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYGSNKKTRHMLP 244
           IVKKRTK F RHQSD + ++  +WRKPRGID+ VRRRF+G   MP IGYG+NKKTR+ +P
Sbjct: 6   IVKKRTKPFKRHQSDLFKRVGESWRKPRGIDSCVRRRFRGTISMPKIGYGNNKKTRYCMP 65

Query: 245 NGFLRS*FTMLKSWKS**CKTGST--AQRSLMVSLRRSGS*FVERAQQLSIRVTNAAARL 418
           NG L++      S          T  A+ +  VS R+     VE+A+ L ++VTNA A++
Sbjct: 66  NG-LKAFLVRNVSDVELLLMHNKTYAAEIASNVSARKRVE-IVEKARALGVKVTNAGAKV 123

Query: 419 RSQE 430
           RSQE
Sbjct: 124 RSQE 127


>SPBC6B1.10 |prp17||splicing factor Prp17|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 558

 Score = 27.5 bits (58), Expect = 1.1
 Identities = 13/23 (56%), Positives = 17/23 (73%), Gaps = 2/23 (8%)
 Frame = -1

Query: 139 TPIPLKFV--IAIRLMPDKSLRP 77
           TP+P+KFV  IA+  MP  +LRP
Sbjct: 427 TPVPIKFVADIAMHSMPRVALRP 449


>SPAC139.03 |||transcription factor, zf-fungal binuclear cluster
           type |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 625

 Score = 27.1 bits (57), Expect = 1.5
 Identities = 17/48 (35%), Positives = 22/48 (45%), Gaps = 3/48 (6%)
 Frame = -1

Query: 406 RIRHSDAELLGSFHESASAS---SKRHHERSLRSTSCFASSRFPAL*H 272
           R+   D EL   F E    +   S   HER +R    + S+RFP L H
Sbjct: 424 RLLELDRELSNFFKELIEVNIPTSNTMHERHIRLLVSYLSNRFPILLH 471


>SPAC1250.04c |atl1||alkyltransferase-like protein
           Atl1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 108

 Score = 27.1 bits (57), Expect = 1.5
 Identities = 14/32 (43%), Positives = 20/32 (62%)
 Frame = +1

Query: 13  VSKRNIQDGYKTCLQADNRQKEDEEIYQASIG 108
           +SKR+I  G +   Q D  ++E  EIYQ S+G
Sbjct: 66  ISKRDISAGEQR--QKDRLEEEGVEIYQTSLG 95


>SPBC354.02c |sec61||translocon alpha subunit
           Sec61|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 479

 Score = 25.4 bits (53), Expect = 4.5
 Identities = 17/57 (29%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
 Frame = -2

Query: 447 NYIY-SFSWERRRAAAFVTLMLSCWALSTNQLPLLRRDTMSDLCAVLPVLHHQDFQL 280
           N++Y  F+W+ + AA +     S    S  QLP L  +  + L     V++ QDF++
Sbjct: 217 NFVYVMFTWDNKAAALYQAFFRSGLTSSQIQLPNL-WNFFATLLVFGVVIYLQDFRV 272


>SPBC336.10c |tif512||translation initiation
           factor|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 157

 Score = 25.0 bits (52), Expect = 5.9
 Identities = 9/29 (31%), Positives = 15/29 (51%)
 Frame = +1

Query: 25  NIQDGYKTCLQADNRQKEDEEIYQASIGS 111
           NI DGY   +  D   K+D  + +  +G+
Sbjct: 95  NIDDGYLNLMTTDGTTKDDVRLPEGELGN 123


>SPBC21C3.20c |git1||C2 domain protein Git1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1098

 Score = 25.0 bits (52), Expect = 5.9
 Identities = 14/42 (33%), Positives = 20/42 (47%)
 Frame = +2

Query: 89  FIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYG 214
           F  H  D YD+L  NW++   ++    +RFK      NI  G
Sbjct: 822 FCVHTRDVYDELVPNWKETYQMEITNVKRFKLIIYQRNIHTG 863


>SPAC26H5.10c |tif51||translation initiation factor
           eIF5A|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 157

 Score = 25.0 bits (52), Expect = 5.9
 Identities = 9/29 (31%), Positives = 15/29 (51%)
 Frame = +1

Query: 25  NIQDGYKTCLQADNRQKEDEEIYQASIGS 111
           NI DGY   +  D   K+D  + +  +G+
Sbjct: 95  NIDDGYLNLMTTDGTTKDDVRLPEGELGN 123


>SPAC15A10.15 |sgo2||shugoshin Sgo2|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 647

 Score = 24.6 bits (51), Expect = 7.9
 Identities = 16/50 (32%), Positives = 23/50 (46%)
 Frame = +2

Query: 56  RPTIVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNI 205
           R T VK+R K  I+  S+    +     KP G   R R+  K  Y +P +
Sbjct: 540 RETKVKRRRKARIQETSEESTVVNEPNEKPDGRSRRERK--KVNYALPGL 587


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,849,542
Number of Sequences: 5004
Number of extensions: 36274
Number of successful extensions: 120
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 115
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 118
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 184476110
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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