BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30433.Seq (904 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3H5.10 |rpl3202|rpl32-2, rpl32|60S ribosomal protein L32|Sch... 97 4e-21 SPBC16C6.11 |rpl3201|rpl32-1|60S ribosomal protein L32|Schizosac... 94 2e-20 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 31 0.30 SPBC6B1.10 |prp17||splicing factor Prp17|Schizosaccharomyces pom... 27 2.8 SPBC21.02 |||TLDc domain protein 2|Schizosaccharomyces pombe|chr... 27 3.6 SPAC1250.04c |atl1||alkyltransferase-like protein Atl1|Schizosac... 27 3.6 SPBC1105.10 |rav1||RAVE complex subunit Rav1 |Schizosaccharomyce... 26 8.4 SPBC1709.01 |chs2|SPBC1734.17|chitin synthase homolog Chs2|Schiz... 26 8.4 >SPAC3H5.10 |rpl3202|rpl32-2, rpl32|60S ribosomal protein L32|Schizosaccharomyces pombe|chr 1|||Manual Length = 127 Score = 96.7 bits (230), Expect = 4e-21 Identities = 41/62 (66%), Positives = 51/62 (82%) Frame = +2 Query: 65 IVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYGSNKKTRHMLP 244 I+KKRTK F RHQSDR+ ++ +WRKPRGID+ VRRRF+G MP IGYG+NKKTR+ +P Sbjct: 6 IIKKRTKPFKRHQSDRFKRVGESWRKPRGIDSCVRRRFRGTISMPKIGYGNNKKTRYCMP 65 Query: 245 NG 250 NG Sbjct: 66 NG 67 Score = 68.9 bits (161), Expect = 9e-13 Identities = 32/63 (50%), Positives = 46/63 (73%) Frame = +1 Query: 241 PKWIPKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIVERAQQLSIRVTNAAARLR 420 P + LV NV ++E+L+M N+ Y AEIA VS++KR IVE+A+ L ++VTNA A++R Sbjct: 65 PNGLKAFLVRNVSDVELLLMHNKTYAAEIAGNVSARKRVEIVEKARALGVKVTNAGAKVR 124 Query: 421 SQE 429 SQE Sbjct: 125 SQE 127 >SPBC16C6.11 |rpl3201|rpl32-1|60S ribosomal protein L32|Schizosaccharomyces pombe|chr 2|||Manual Length = 127 Score = 94.3 bits (224), Expect = 2e-20 Identities = 41/62 (66%), Positives = 50/62 (80%) Frame = +2 Query: 65 IVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYGSNKKTRHMLP 244 IVKKRTK F RHQSD + ++ +WRKPRGID+ VRRRF+G MP IGYG+NKKTR+ +P Sbjct: 6 IVKKRTKPFKRHQSDLFKRVGESWRKPRGIDSCVRRRFRGTISMPKIGYGNNKKTRYCMP 65 Query: 245 NG 250 NG Sbjct: 66 NG 67 Score = 69.3 bits (162), Expect = 7e-13 Identities = 32/63 (50%), Positives = 46/63 (73%) Frame = +1 Query: 241 PKWIPKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIVERAQQLSIRVTNAAARLR 420 P + LV NV ++E+L+M N+ Y AEIA VS++KR IVE+A+ L ++VTNA A++R Sbjct: 65 PNGLKAFLVRNVSDVELLLMHNKTYAAEIASNVSARKRVEIVEKARALGVKVTNAGAKVR 124 Query: 421 SQE 429 SQE Sbjct: 125 SQE 127 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 30.7 bits (66), Expect = 0.30 Identities = 20/65 (30%), Positives = 32/65 (49%), Gaps = 5/65 (7%) Frame = +2 Query: 485 PVPNSPYSSRITIHWPSFYNVVTGKTLALPNLIALQ-----HIPLSPAGVIAKRPAPIAL 649 P+P P S ++ H + + +L N I+L ++PLSP A+ P+PI L Sbjct: 170 PLPRPPLPSSVSSHSSPYSTTSSTSLYSLYNDISLSCSPEPYLPLSPTRSPARTPSPIRL 229 Query: 650 PNSCA 664 +S A Sbjct: 230 YSSDA 234 >SPBC6B1.10 |prp17||splicing factor Prp17|Schizosaccharomyces pombe|chr 2|||Manual Length = 558 Score = 27.5 bits (58), Expect = 2.8 Identities = 13/23 (56%), Positives = 17/23 (73%), Gaps = 2/23 (8%) Frame = -1 Query: 139 TPIPLKFV--IAIRLMPDKSLRP 77 TP+P+KFV IA+ MP +LRP Sbjct: 427 TPVPIKFVADIAMHSMPRVALRP 449 >SPBC21.02 |||TLDc domain protein 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 511 Score = 27.1 bits (57), Expect = 3.6 Identities = 12/32 (37%), Positives = 19/32 (59%) Frame = -2 Query: 405 RIRHSDAELLGSFHDQLPLLRRDTMSDLCAVL 310 R+ HSD + + +F DQ +RR + LC +L Sbjct: 148 RLIHSDVQNISNFFDQNFGIRRSNIYQLCLLL 179 >SPAC1250.04c |atl1||alkyltransferase-like protein Atl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 108 Score = 27.1 bits (57), Expect = 3.6 Identities = 14/32 (43%), Positives = 20/32 (62%) Frame = +1 Query: 13 VSKRNIQDGYKTCLQADNRQKEDEEIYQASIG 108 +SKR+I G + Q D ++E EIYQ S+G Sbjct: 66 ISKRDISAGEQR--QKDRLEEEGVEIYQTSLG 95 >SPBC1105.10 |rav1||RAVE complex subunit Rav1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1297 Score = 25.8 bits (54), Expect = 8.4 Identities = 18/62 (29%), Positives = 32/62 (51%), Gaps = 1/62 (1%) Frame = +1 Query: 247 WIPKVLVHNVKEL-EILMMQNRKYCAEIAHGVSSKKRKLIVERAQQLSIRVTNAAARLRS 423 W+ +VL + E+ EI +Q A + G +SKK ++ E ++LSI T ++ Sbjct: 579 WMARVLGDDKAEISEISKVQTSIKSATMIKGSTSKKVAVVSENNRRLSIFDTRSSEFSEK 638 Query: 424 QE 429 +E Sbjct: 639 EE 640 >SPBC1709.01 |chs2|SPBC1734.17|chitin synthase homolog Chs2|Schizosaccharomyces pombe|chr 2|||Manual Length = 926 Score = 25.8 bits (54), Expect = 8.4 Identities = 11/27 (40%), Positives = 13/27 (48%) Frame = -1 Query: 673 PFXCATVGKGDRCGPLRYYASWRKGDV 593 P C V G R GP Y +W+ DV Sbjct: 392 PKVCLFVRNGARLGPTSIYHAWKAFDV 418 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,542,616 Number of Sequences: 5004 Number of extensions: 70375 Number of successful extensions: 169 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 166 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 169 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 456499320 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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