Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30432.Seq
(857 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC13G6.02c |rps101|rps1-1, rps3a-1|40S ribosomal protein S3a|S... 73 7e-14
SPAC22H12.04c |rps102|rps1-2, rps3a-2|40S ribosomal protein S3a|... 69 9e-13
SPAC23E2.02 |lsd2|swm2, saf140|histone demethylase SWIRM2 |Schiz... 29 1.1
SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|... 28 2.0
SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces pom... 26 7.9
>SPAC13G6.02c |rps101|rps1-1, rps3a-1|40S ribosomal protein
S3a|Schizosaccharomyces pombe|chr 1|||Manual
Length = 252
Score = 72.5 bits (170), Expect = 7e-14
Identities = 65/224 (29%), Positives = 102/224 (45%), Gaps = 9/224 (4%)
Frame = -3
Query: 762 RLSTPFIRKGXVRCQGPVYVQQEASRHHACXPSPGXEXCFXGLKGRVFEVSLADLQADID 583
R+ PF RK + P + + + + G + LKGR+ EVSLADLQ D
Sbjct: 19 RVVDPFSRKDWYDIKAPAFFEVKNVGKTLVNRTAGLKNANDSLKGRILEVSLADLQK--D 76
Query: 582 AERSFRKFRLIAEYVQGRNVLCNFQAW--TSQPISSGGWLKNGRLSSKPTLM*RQPMDTF 409
E SFRK +L E +QG++ L +F + TS + S + + T+ + D +
Sbjct: 77 EEHSFRKVKLRVEDIQGKSCLTSFNGFDMTSDKLRSLVRKWQSTIEANQTI---KTTDGY 133
Query: 408 YVSSALVSXXXXXXXXXXXXXXAHSGQSNQKENV*NHYTRRH*L*TQGGG---EQLIPDS 238
++ QS+Q + H + Q G ++L+
Sbjct: 134 LCRIFVIGFTSRRVNQVKKTTY---AQSSQIRAI--HQKMFQVIQNQANGCSMKELVQKL 188
Query: 237 IAKDI----EKACHGIYPLRDVCIRKVKVLKRPRFEISKLMELH 118
I + I EKA + IYPL++V +RKVK+LK P+ + KL+ELH
Sbjct: 189 IPEVIGRAIEKATNNIYPLQNVFVRKVKILKAPKHDAQKLLELH 232
Score = 69.7 bits (163), Expect = 5e-13
Identities = 30/58 (51%), Positives = 41/58 (70%)
Frame = -2
Query: 526 CALQLPGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCIGFTNKDSLSQRKT 353
C G D+T+DKLR +V+KWQ+ IEAN +KTTDGY+ R+F IGFT++ +KT
Sbjct: 96 CLTSFNGFDMTSDKLRSLVRKWQSTIEANQTIKTTDGYLCRIFVIGFTSRRVNQVKKT 153
Score = 31.1 bits (67), Expect = 0.21
Identities = 12/31 (38%), Positives = 20/31 (64%)
Frame = -1
Query: 350 YAQHTQVRAIRKKMCEIITRDVTNSELREVV 258
YAQ +Q+RAI +KM ++I ++E+V
Sbjct: 155 YAQSSQIRAIHQKMFQVIQNQANGCSMKELV 185
>SPAC22H12.04c |rps102|rps1-2, rps3a-2|40S ribosomal protein
S3a|Schizosaccharomyces pombe|chr 1|||Manual
Length = 252
Score = 68.9 bits (161), Expect = 9e-13
Identities = 30/58 (51%), Positives = 41/58 (70%)
Frame = -2
Query: 526 CALQLPGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCIGFTNKDSLSQRKT 353
C G+ +T+DKLR +V+KWQT IEA+ +KTTDGY+ RVF IGFT + + +KT
Sbjct: 96 CLTSFNGLSITSDKLRSLVRKWQTTIEADQTIKTTDGYLCRVFVIGFTRRRANQVKKT 153
Score = 56.8 bits (131), Expect = 4e-09
Identities = 23/47 (48%), Positives = 37/47 (78%)
Frame = -3
Query: 258 EQLIPDSIAKDIEKACHGIYPLRDVCIRKVKVLKRPRFEISKLMELH 118
++LIP+ I ++IE+A I+PL++V +RKVK+LK P+ + KL+ELH
Sbjct: 186 QKLIPEVIGREIERATGSIFPLQNVLVRKVKILKAPKHDAQKLLELH 232
Score = 51.2 bits (117), Expect = 2e-07
Identities = 30/84 (35%), Positives = 44/84 (52%)
Frame = -3
Query: 762 RLSTPFIRKGXVRCQGPVYVQQEASRHHACXPSPGXEXCFXGLKGRVFEVSLADLQADID 583
R+ PF RK + P + + + + G + LKGR+ EVSLADLQ D
Sbjct: 19 RVVDPFSRKEWYDIKAPAFFEVKNVGKTLVNRTAGLKNANDSLKGRILEVSLADLQK--D 76
Query: 582 AERSFRKFRLIAEYVQGRNVLCNF 511
E +FRK +L E +QG++ L +F
Sbjct: 77 EEHAFRKVKLRVEDIQGKSCLTSF 100
Score = 35.1 bits (77), Expect = 0.013
Identities = 14/31 (45%), Positives = 23/31 (74%)
Frame = -1
Query: 350 YAQHTQVRAIRKKMCEIITRDVTNSELREVV 258
YAQ +Q+RAIR+KM ++I ++ +RE+V
Sbjct: 155 YAQSSQIRAIRQKMFQVIQNQTSSCSMRELV 185
>SPAC23E2.02 |lsd2|swm2, saf140|histone demethylase SWIRM2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1273
Score = 28.7 bits (61), Expect = 1.1
Identities = 17/47 (36%), Positives = 22/47 (46%)
Frame = +3
Query: 129 STSRSRNGASSTLSPFGCKHRAEGRCHGRPSRCPWQWSQESTVHHLP 269
S + S N +S L R EG PSR QWS +S+ H +P
Sbjct: 330 SAATSGNISSPALFDSELGARPEGSVAIEPSRVLLQWSSQSSSHTIP 376
>SPBC1826.01c |mot1||TATA-binding protein associated factor
Mot1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1953
Score = 27.9 bits (59), Expect = 2.0
Identities = 14/24 (58%), Positives = 15/24 (62%)
Frame = +3
Query: 126 PSTSRSRNGASSTLSPFGCKHRAE 197
PSTSR RN S LS KHRA+
Sbjct: 736 PSTSRERNNNISELSNSRTKHRAK 759
>SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 772
Score = 25.8 bits (54), Expect = 7.9
Identities = 18/59 (30%), Positives = 27/59 (45%), Gaps = 5/59 (8%)
Frame = -2
Query: 520 LQLPGMDLTTDKLRWMVKKWQTLIEANI-----DVKTTDGYVLRVFCIGFTNKDSLSQR 359
L+L G+DL W + + + NI D+ DGYV +F + T SL Q+
Sbjct: 654 LELLGVDLDAISFHWFLSVYTDTLPTNISFRIFDMLFCDGYVC-LFRVALTILKSLKQQ 711
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,371,977
Number of Sequences: 5004
Number of extensions: 67168
Number of successful extensions: 189
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 185
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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