Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30429.Seq
         (712 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccha...   114   1e-26
SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosacchar...   114   1e-26
SPAC6G9.02c |nop9||RNA-binding protein Nop9|Schizosaccharomyces ...    33   0.053
SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki...    29   0.66 
SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|...    27   2.6  
SPAC139.03 |||transcription factor, zf-fungal binuclear cluster ...    26   6.1  
SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc...    26   6.1  

>SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein
           S8|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 200

 Score =  114 bits (275), Expect = 1e-26
 Identities = 52/75 (69%), Positives = 61/75 (81%)
 Frame = +1

Query: 31  MGISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRXHSVRSRGGNTKYRALRL 210
           MGI+RD  HKR ATG KRA  RKKRK+ELGR  +NTR+GP+R H VR RGGN K+RALRL
Sbjct: 1   MGITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRL 60

Query: 211 DTGNFSWGSECSTRK 255
           D+GNFSWGSE  ++K
Sbjct: 61  DSGNFSWGSEGVSKK 75



 Score = 77.8 bits (183), Expect = 1e-15
 Identities = 42/87 (48%), Positives = 55/87 (63%)
 Frame = +3

Query: 246 NSQTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRKKGAKLTEA 425
           + +TRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY + +G  KG K T  
Sbjct: 73  SKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGILMG-SKGKKATST 131

Query: 426 EEAIINKKRSQKTARKYLARQRLAKVE 506
                   +S+   RK+ AR   +KV+
Sbjct: 132 P-----NPKSKHVQRKHSARLGDSKVD 153



 Score = 67.7 bits (158), Expect = 2e-12
 Identities = 31/46 (67%), Positives = 36/46 (78%)
 Frame = +2

Query: 509 ALEEQFHTGRLLACVASRPGQCGRADGYILEGKELEFYLRKIKSKR 646
           ALE QF  GRL A V+SRPGQ GR DGYILEG+EL FYLR++  K+
Sbjct: 155 ALETQFAAGRLYAVVSSRPGQSGRCDGYILEGEELHFYLRRMAPKK 200


>SPAC521.05 |rps802|rps8-2|40S ribosomal protein
           S8|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 200

 Score =  114 bits (275), Expect = 1e-26
 Identities = 52/75 (69%), Positives = 61/75 (81%)
 Frame = +1

Query: 31  MGISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRXHSVRSRGGNTKYRALRL 210
           MGI+RD  HKR ATG KRA  RKKRK+ELGR  +NTR+GP+R H VR RGGN K+RALRL
Sbjct: 1   MGITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRL 60

Query: 211 DTGNFSWGSECSTRK 255
           D+GNFSWGSE  ++K
Sbjct: 61  DSGNFSWGSEGVSKK 75



 Score = 77.0 bits (181), Expect = 2e-15
 Identities = 42/87 (48%), Positives = 55/87 (63%)
 Frame = +3

Query: 246 NSQTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRKKGAKLTEA 425
           + +TRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY + +G  KG K T  
Sbjct: 73  SKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGILMG-SKGKKATAT 131

Query: 426 EEAIINKKRSQKTARKYLARQRLAKVE 506
                   +S+   RK+ AR   +KV+
Sbjct: 132 P-----TPKSKHVQRKHSARLGDSKVD 153



 Score = 67.7 bits (158), Expect = 2e-12
 Identities = 31/46 (67%), Positives = 36/46 (78%)
 Frame = +2

Query: 509 ALEEQFHTGRLLACVASRPGQCGRADGYILEGKELEFYLRKIKSKR 646
           ALE QF  GRL A V+SRPGQ GR DGYILEG+EL FYLR++  K+
Sbjct: 155 ALETQFAAGRLYAVVSSRPGQSGRCDGYILEGEELHFYLRRMAPKK 200


>SPAC6G9.02c |nop9||RNA-binding protein Nop9|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 655

 Score = 32.7 bits (71), Expect = 0.053
 Identities = 16/40 (40%), Positives = 21/40 (52%)
 Frame = -3

Query: 623 DRTRVLCLLRCNHRRDHTDLGDSPRKPANALCGIALLEPL 504
           D   + CLLR  H+RD  +  +  RK  N L G  LLE +
Sbjct: 434 DANIIPCLLRSKHKRDKANPENKERKLVNNLLGAQLLEEM 473


>SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase
            Win1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1436

 Score = 29.1 bits (62), Expect = 0.66
 Identities = 21/74 (28%), Positives = 32/74 (43%), Gaps = 2/74 (2%)
 Frame = +1

Query: 43   RDHWHKRRATGGKRAPIRKKRKY--ELGRPAANTRLGPQRXHSVRSRGGNTKYRALRLDT 216
            RD  +K +  G       K+ +   EL    +N  +  Q+   + S    T YRA+ LDT
Sbjct: 1083 RDRLYKSQLIGRVLDDTTKENRLLKELASSKSNITIRWQQGGLIGSGSFGTVYRAVNLDT 1142

Query: 217  GNFSWGSECSTRKP 258
            G+     E +  KP
Sbjct: 1143 GDLMAVKEVALHKP 1156


>SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 207

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 17/54 (31%), Positives = 25/54 (46%)
 Frame = +1

Query: 46  DHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRXHSVRSRGGNTKYRALR 207
           + W K   T  K+    +KRK  L R A   RL  +   S+ S+GG    +A +
Sbjct: 32  EKWSKGVKTN-KKEQEAEKRKAALERKAERERLEKEEMESLPSKGGKGSKKAAK 84


>SPAC139.03 |||transcription factor, zf-fungal binuclear cluster
           type |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 625

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 15/39 (38%), Positives = 19/39 (48%)
 Frame = +1

Query: 19  DPTKMGISRDHWHKRRATGGKRAPIRKKRKYELGRPAAN 135
           + TK G  +     RRA     A  RKK K + GRP +N
Sbjct: 3   ETTKSGSKKSGQTSRRAIHSCLACRRKKLKCDHGRPCSN 41


>SPBC16H5.10c |prp43||ATP-dependent RNA helicase
           Prp43|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 735

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 11/25 (44%), Positives = 15/25 (60%)
 Frame = -1

Query: 367 YHCLNGVASTTTIAFLTRVFVRTNS 293
           ++C N V S T +  +  VFVR NS
Sbjct: 518 FYCSNEVLSLTALLSVPNVFVRPNS 542


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,721,219
Number of Sequences: 5004
Number of extensions: 53703
Number of successful extensions: 148
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)