Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30421.Seq
(806 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g80460.1 68414.m09423 glycerol kinase, putative similar to gl... 48 6e-06
At4g30310.3 68417.m04309 ribitol kinase, putative similar to rib... 35 0.073
At4g30310.2 68417.m04308 ribitol kinase, putative similar to rib... 35 0.073
At5g54650.2 68418.m06805 formin homology 2 domain-containing pro... 34 0.13
At5g54650.1 68418.m06804 formin homology 2 domain-containing pro... 34 0.13
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 31 1.2
At4g24900.1 68417.m03564 expressed protein 30 2.1
At1g70620.2 68414.m08137 cyclin-related contains weak similarity... 30 2.1
At5g11610.2 68418.m01356 exostosin family protein contains Pfam ... 29 2.7
At5g11610.1 68418.m01355 exostosin family protein contains Pfam ... 29 2.7
At1g16610.1 68414.m01989 arginine/serine-rich protein, putative ... 29 2.7
At1g74260.1 68414.m08600 AIR synthase-related family protein con... 29 4.8
At1g27750.1 68414.m03391 ubiquitin system component Cue domain-c... 29 4.8
At5g52040.2 68418.m06459 arginine/serine-rich splicing factor RS... 28 6.3
At5g52040.1 68418.m06458 arginine/serine-rich splicing factor RS... 28 6.3
At5g27420.1 68418.m03273 zinc finger (C3HC4-type RING finger) fa... 28 6.3
At3g02830.1 68416.m00275 zinc finger (CCCH-type) family protein ... 28 6.3
At2g40070.1 68415.m04923 expressed protein 28 6.3
At1g10520.1 68414.m01184 DNA polymerase lambda (POLL) identical ... 28 6.3
At5g24480.1 68418.m02885 hypothetical protein similar to unknown... 28 8.4
At3g14130.1 68416.m01787 (S)-2-hydroxy-acid oxidase, peroxisomal... 28 8.4
At1g16610.2 68414.m01990 arginine/serine-rich protein, putative ... 28 8.4
>At1g80460.1 68414.m09423 glycerol kinase, putative similar to
glycerol kinase (ATP:glycerol 3-phosphotransferase,
Glycerokinase, GK)[Mycobacterium tuberculosis]
Swiss-Prot:O69664
Length = 522
Score = 48.4 bits (110), Expect = 6e-06
Identities = 21/36 (58%), Positives = 29/36 (80%)
Frame = -1
Query: 509 VQMQADLLGIPVIRPLMMESTALGAAIVAGRAMRVW 402
+Q+QADL+G PV+RP+ +E+TALGAA AG A+ W
Sbjct: 438 MQIQADLMGSPVVRPVDIETTALGAAYAAGLAVGFW 473
Score = 37.5 bits (83), Expect = 0.010
Identities = 19/57 (33%), Positives = 33/57 (57%)
Frame = -2
Query: 763 AXMEIEASISFVPAFNGLYSPILEKDAQGK*CAV*AKGLDVSILSGPALEAVCHQTR 593
A ++ + FVPAFNGL++P +DA+G C + + S ++ LE++C Q +
Sbjct: 345 AMVDSTGGVYFVPAFNGLFAPWWREDARGV-CIGITRFTNKSHIARAVLESMCFQVK 400
>At4g30310.3 68417.m04309 ribitol kinase, putative similar to
ribitol kinase [Klebsiella pneumoniae]
gi|2905643|gb|AAC26495
Length = 451
Score = 34.7 bits (76), Expect = 0.073
Identities = 16/38 (42%), Positives = 24/38 (63%)
Frame = -1
Query: 509 VQMQADLLGIPVIRPLMMESTALGAAIVAGRAMRVWPT 396
+Q AD++G P+I P ES LGAAI+ A + +P+
Sbjct: 361 IQEHADIVGCPIILPRESESVLLGAAILGAVAGKNYPS 398
>At4g30310.2 68417.m04308 ribitol kinase, putative similar to
ribitol kinase [Klebsiella pneumoniae]
gi|2905643|gb|AAC26495
Length = 579
Score = 34.7 bits (76), Expect = 0.073
Identities = 16/38 (42%), Positives = 24/38 (63%)
Frame = -1
Query: 509 VQMQADLLGIPVIRPLMMESTALGAAIVAGRAMRVWPT 396
+Q AD++G P+I P ES LGAAI+ A + +P+
Sbjct: 489 IQEHADIVGCPIILPRESESVLLGAAILGAVAGKNYPS 526
>At5g54650.2 68418.m06805 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 900
Score = 33.9 bits (74), Expect = 0.13
Identities = 18/40 (45%), Positives = 24/40 (60%), Gaps = 3/40 (7%)
Frame = +1
Query: 514 LSSAPSLRRRGVAS---GARSLQPSRPPRRASGDTRPPAR 624
LSS + RR +A+ + S PSRPP+R+ G RPP R
Sbjct: 127 LSSMLDIPRRNLATKPGSSPSPSPSRPPKRSRGPPRPPTR 166
>At5g54650.1 68418.m06804 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 900
Score = 33.9 bits (74), Expect = 0.13
Identities = 18/40 (45%), Positives = 24/40 (60%), Gaps = 3/40 (7%)
Frame = +1
Query: 514 LSSAPSLRRRGVAS---GARSLQPSRPPRRASGDTRPPAR 624
LSS + RR +A+ + S PSRPP+R+ G RPP R
Sbjct: 127 LSSMLDIPRRNLATKPGSSPSPSPSRPPKRSRGPPRPPTR 166
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 30.7 bits (66), Expect = 1.2
Identities = 16/36 (44%), Positives = 18/36 (50%)
Frame = +1
Query: 517 SSAPSLRRRGVASGARSLQPSRPPRRASGDTRPPAR 624
S APS RRR + AR + PP R PPAR
Sbjct: 303 SPAPSRRRRSPSPPARRRRSPSPPARRRRSPSPPAR 338
Score = 28.3 bits (60), Expect = 6.3
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +1
Query: 523 APSLRRRGVASGARSLQPSRPPRRASGDTRPPARAL 630
+PS+ +R + PS PPRRA + PPA+ L
Sbjct: 444 SPSIEQRLPSPPVAQRLPSPPPRRAGLPSPPPAQRL 479
Score = 27.9 bits (59), Expect = 8.4
Identities = 14/35 (40%), Positives = 17/35 (48%)
Frame = +1
Query: 520 SAPSLRRRGVASGARSLQPSRPPRRASGDTRPPAR 624
S P+ RRR + AR + PP R PPAR
Sbjct: 314 SPPARRRRSPSPPARRRRSPSPPARRHRSPTPPAR 348
>At4g24900.1 68417.m03564 expressed protein
Length = 421
Score = 29.9 bits (64), Expect = 2.1
Identities = 19/52 (36%), Positives = 25/52 (48%), Gaps = 1/52 (1%)
Frame = -3
Query: 510 CADAG*FTGYTSHSSPHDGK-YSPGGGHCRRSRNACVAHDNTQPTGRYIPAG 358
C ++G G+ HS P K YS G +C + N V+ D Q G Y P G
Sbjct: 224 CDESG--NGFGEHSIPCRSKDYSGNGNYCTQE-NYQVSQDKKQIDGSYNPPG 272
>At1g70620.2 68414.m08137 cyclin-related contains weak similarity to
Swiss-Prot:P35662 cylicin I (Multiple-band polypeptide
I) [Bos taurus]
Length = 884
Score = 29.9 bits (64), Expect = 2.1
Identities = 23/74 (31%), Positives = 30/74 (40%), Gaps = 1/74 (1%)
Frame = +2
Query: 398 WATHALRDLRQWPPPGLYFPS*GDE*LVYPVNQPASAQN*VLRHPSV-GEELPQGRAVCS 574
+ TH +D Q PG+ + + V P QP N + S G LPQ S
Sbjct: 181 YPTHGYQDFHQQAVPGVSYQQ-HQQFPVPPTTQPERYPNYATGNESFPGVGLPQENLPTS 239
Query: 575 HRVRHGARLVTHGL 616
+ RLVTH L
Sbjct: 240 SAIHQQERLVTHKL 253
>At5g11610.2 68418.m01356 exostosin family protein contains Pfam
domain, PF03016: Exostosin family
Length = 453
Score = 29.5 bits (63), Expect = 2.7
Identities = 22/69 (31%), Positives = 30/69 (43%), Gaps = 6/69 (8%)
Frame = +1
Query: 523 APSLRRRG--VASGARSLQPSRPPRRAS----GDTRPPARALTIC*RRVLSLKPHITSPG 684
A RRR AS LQP P R T+PP+ ++I + LK H
Sbjct: 106 ASGQRRRSNETASSLHPLQPKIPQIRKKYPHRSITKPPSIVISIKQMNNMILKRHNDPKN 165
Query: 685 RLSPIWANK 711
L+P+W +K
Sbjct: 166 SLAPLWGSK 174
>At5g11610.1 68418.m01355 exostosin family protein contains Pfam
domain, PF03016: Exostosin family
Length = 546
Score = 29.5 bits (63), Expect = 2.7
Identities = 22/69 (31%), Positives = 30/69 (43%), Gaps = 6/69 (8%)
Frame = +1
Query: 523 APSLRRRG--VASGARSLQPSRPPRRAS----GDTRPPARALTIC*RRVLSLKPHITSPG 684
A RRR AS LQP P R T+PP+ ++I + LK H
Sbjct: 106 ASGQRRRSNETASSLHPLQPKIPQIRKKYPHRSITKPPSIVISIKQMNNMILKRHNDPKN 165
Query: 685 RLSPIWANK 711
L+P+W +K
Sbjct: 166 SLAPLWGSK 174
>At1g16610.1 68414.m01989 arginine/serine-rich protein, putative
(SR45) similar to arginine/serine-rich protein
GI:6601502 from [Arabidopsis thaliana]
Length = 414
Score = 29.5 bits (63), Expect = 2.7
Identities = 23/59 (38%), Positives = 26/59 (44%), Gaps = 4/59 (6%)
Frame = -1
Query: 695 GERRPGEVM----CGLSERTRRQHIVRARAGGRVSPDARRGGRDGCRLRAPEATPRRRR 531
G RRP E GLS R R R + R SPD+ R G +R TP RRR
Sbjct: 211 GPRRPRETSPQRKTGLSPRRRSPLPRRGLSPRRRSPDSPHRRRPGSPIRRRGDTPPRRR 269
>At1g74260.1 68414.m08600 AIR synthase-related family protein
contains Pfam profiles: PF00586 AIR synthase related
protein, N-terminal domain, PF02769 AIR synthase related
protein, C-terminal domain
Length = 1387
Score = 28.7 bits (61), Expect = 4.8
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = -1
Query: 608 VSPDARRGGRDGCRLRAPEATPRRRRDGAELSSV 507
V+PD + GG DG L A +RR G+ L+ V
Sbjct: 898 VTPDLKLGGDDGILLHVDLAKGKRRLGGSALAQV 931
>At1g27750.1 68414.m03391 ubiquitin system component Cue
domain-containing protein very low similarity to ASC-1
complex subunit P100 [Homo sapiens] GI:12061187; contains
Pfam profile PF02845: CUE domain
Length = 1973
Score = 28.7 bits (61), Expect = 4.8
Identities = 20/69 (28%), Positives = 32/69 (46%), Gaps = 5/69 (7%)
Frame = -2
Query: 388 PAHRPIHSCRLLPMKNEKCGEFVGKKP*INAWDGPILK--MNMST---QKIR*NYCSFAA 224
P P+ S RL PM + CG K+ + W G + K ++ ST Q++ + C +
Sbjct: 899 PPFSPLLSPRLPPMVTQLCGSEASKQNIGHQWQGALSKSGVHYSTIIAQRLESDICKYII 958
Query: 223 GTFFPRNRP 197
G+ P P
Sbjct: 959 GSPEPVQWP 967
>At5g52040.2 68418.m06459 arginine/serine-rich splicing factor RSP41
(RSP41) nearly identical to SP|P92966
Arginine/serine-rich splicing factor RSP41 {Arabidopsis
thaliana}
Length = 357
Score = 28.3 bits (60), Expect = 6.3
Identities = 21/70 (30%), Positives = 28/70 (40%)
Frame = +1
Query: 409 RIARPATMAAPRAVLSIMRGRMTGIPSKSACICTELSSAPSLRRRGVASGARSLQPSRPP 588
R A P R RGR + P K A + + RR VAS +R P
Sbjct: 207 RGASPVAHKRERTSPDYGRGRRSPSPYKRARLSPDYKR-DDRRRERVASPENGAVRNRSP 265
Query: 589 RRASGDTRPP 618
R+ G++R P
Sbjct: 266 RKGRGESRSP 275
>At5g52040.1 68418.m06458 arginine/serine-rich splicing factor RSP41
(RSP41) nearly identical to SP|P92966
Arginine/serine-rich splicing factor RSP41 {Arabidopsis
thaliana}
Length = 356
Score = 28.3 bits (60), Expect = 6.3
Identities = 21/70 (30%), Positives = 28/70 (40%)
Frame = +1
Query: 409 RIARPATMAAPRAVLSIMRGRMTGIPSKSACICTELSSAPSLRRRGVASGARSLQPSRPP 588
R A P R RGR + P K A + + RR VAS +R P
Sbjct: 207 RGASPVAHKRERTSPDYGRGRRSPSPYKRARLSPDYKR-DDRRRERVASPENGAVRNRSP 265
Query: 589 RRASGDTRPP 618
R+ G++R P
Sbjct: 266 RKGRGESRSP 275
>At5g27420.1 68418.m03273 zinc finger (C3HC4-type RING finger)
family protein similar to RING-H2 zinc finger protein
ATL6 [Arabidopsis thaliana]
gi|4928403|gb|AAD33584.1|AF132016_1[4928403]; contains
Pfam domain, PF00097: Zinc finger, C3HC4 type (RING
finger)
Length = 368
Score = 28.3 bits (60), Expect = 6.3
Identities = 19/50 (38%), Positives = 21/50 (42%), Gaps = 1/50 (2%)
Frame = -3
Query: 498 G*FTGYTSH-SSPHDGKYSPGGGHCRRSRNACVAHDNTQPTGRYIPAGSY 352
G FT Y H + DG +P GG RR NA VA T P Y
Sbjct: 60 GFFTVYIRHCTGAVDGSVTPAGGARRRVTNATVARGLDAETIETFPTFVY 109
>At3g02830.1 68416.m00275 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 397
Score = 28.3 bits (60), Expect = 6.3
Identities = 14/46 (30%), Positives = 17/46 (36%)
Frame = -3
Query: 486 GYTSHSSPHDGKYSPGGGHCRRSRNACVAHDNTQPTGRYIPAGSYQ 349
GY S+ D Y GHC+ H QPT +P Q
Sbjct: 127 GYPLRSNEVDCAYFLRTGHCKFGGTCKFNHPQPQPTNMMVPTSGQQ 172
>At2g40070.1 68415.m04923 expressed protein
Length = 607
Score = 28.3 bits (60), Expect = 6.3
Identities = 18/62 (29%), Positives = 28/62 (45%)
Frame = +1
Query: 415 ARPATMAAPRAVLSIMRGRMTGIPSKSACICTELSSAPSLRRRGVASGARSLQPSRPPRR 594
+RPAT+ + A S + S+ LSS+ RR +SG +P+ P R
Sbjct: 133 SRPATLTSRLANSSTESAARNHLTSRQQTSSPGLSSSSGASRRPSSSGGPGSRPATPTGR 192
Query: 595 AS 600
+S
Sbjct: 193 SS 194
>At1g10520.1 68414.m01184 DNA polymerase lambda (POLL) identical to
DNA polymerase lambda GI:12053869 from [Arabidopsis
thaliana]
Length = 529
Score = 28.3 bits (60), Expect = 6.3
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = -3
Query: 552 GNSSPTEGWRRTQFCADAG*FTGYTSHSSPHDGKYSP 442
G+ + +RT++ DAG F G SHS+ SP
Sbjct: 128 GSEDQSSPQKRTRYSPDAGDFKGVESHSNTQGSPDSP 164
>At5g24480.1 68418.m02885 hypothetical protein similar to unknown
protein (gb|AAD32930.1)
Length = 350
Score = 27.9 bits (59), Expect = 8.4
Identities = 14/39 (35%), Positives = 18/39 (46%), Gaps = 3/39 (7%)
Frame = -1
Query: 470 RPLMMESTALGAAIVAGRAMRVWPTTIPSP---PADTFL 363
RP ++ + R R WP T+PSP A TFL
Sbjct: 10 RPHFQSIHFFDCSLTSDRGQRSWPATLPSPQPLSASTFL 48
>At3g14130.1 68416.m01787 (S)-2-hydroxy-acid oxidase, peroxisomal,
putative / glycolate oxidase, putative / short chain
alpha-hydroxy acid oxidase, putative similar to Chain A,
Glycolate Oxidase (E.C.1.1.3.15) Mutant With Tyr 24
Replaced By Phe (Y24f) gi|999542
Length = 363
Score = 27.9 bits (59), Expect = 8.4
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = -1
Query: 539 RRRDGAELSSVQMQADLLGIPVIRPLMMESTAL 441
R R ++S++ M +LG P+ P+M+ TA+
Sbjct: 49 RPRVLVDVSNIDMSTSMLGYPISAPIMIAPTAM 81
>At1g16610.2 68414.m01990 arginine/serine-rich protein, putative
(SR45) similar to arginine/serine-rich protein
GI:6601502 from [Arabidopsis thaliana]
Length = 407
Score = 27.9 bits (59), Expect = 8.4
Identities = 22/55 (40%), Positives = 25/55 (45%)
Frame = -1
Query: 695 GERRPGEVMCGLSERTRRQHIVRARAGGRVSPDARRGGRDGCRLRAPEATPRRRR 531
G RRP E LS R R R + R SPD+ R G +R TP RRR
Sbjct: 211 GPRRPRE---RLSPRRRSPLPRRGLSPRRRSPDSPHRRRPGSPIRRRGDTPPRRR 262
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,398,273
Number of Sequences: 28952
Number of extensions: 395482
Number of successful extensions: 961
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 904
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 961
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1833827200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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