BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30412.Seq (477 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16C6.11 |rpl3201|rpl32-1|60S ribosomal protein L32|Schizosac... 58 7e-10 SPAC3H5.10 |rpl3202|rpl32-2, rpl32|60S ribosomal protein L32|Sch... 57 1e-09 SPBC6B1.10 |prp17||splicing factor Prp17|Schizosaccharomyces pom... 27 1.1 SPAC139.03 |||transcription factor, zf-fungal binuclear cluster ... 27 1.5 SPAC1250.04c |atl1||alkyltransferase-like protein Atl1|Schizosac... 27 1.5 SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar... 25 4.5 SPBC336.10c |tif512||translation initiation factor|Schizosacchar... 25 5.9 SPAC26H5.10c |tif51||translation initiation factor eIF5A|Schizos... 25 5.9 SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces pomb... 25 7.9 >SPBC16C6.11 |rpl3201|rpl32-1|60S ribosomal protein L32|Schizosaccharomyces pombe|chr 2|||Manual Length = 127 Score = 58.0 bits (134), Expect = 7e-10 Identities = 34/88 (38%), Positives = 52/88 (59%), Gaps = 1/88 (1%) Frame = +1 Query: 169 QRFKGQYLMPNIGYGSNKKTRHMLPNGFRRS*FTMLKSWKS**CKTGS-TAQRSLMVSLR 345 +RF+G MP IGYG+NKKTR+ +PNG + + + + A+ + VS R Sbjct: 41 RRFRGTISMPKIGYGNNKKTRYCMPNGLKAFLVRNVSDVELLLMHNKTYAAEIASNVSAR 100 Query: 346 RSGS*FVERAQQLSIRVTNAAARLRSQE 429 + VE+A+ L ++VTNA A++RSQE Sbjct: 101 KRVE-IVEKARALGVKVTNAGAKVRSQE 127 Score = 52.4 bits (120), Expect = 3e-08 Identities = 23/35 (65%), Positives = 28/35 (80%) Frame = +2 Query: 65 IVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVR 169 IVKKRTK F RHQSD + ++ +WRKPRGID+ VR Sbjct: 6 IVKKRTKPFKRHQSDLFKRVGESWRKPRGIDSCVR 40 >SPAC3H5.10 |rpl3202|rpl32-2, rpl32|60S ribosomal protein L32|Schizosaccharomyces pombe|chr 1|||Manual Length = 127 Score = 57.2 bits (132), Expect = 1e-09 Identities = 34/88 (38%), Positives = 52/88 (59%), Gaps = 1/88 (1%) Frame = +1 Query: 169 QRFKGQYLMPNIGYGSNKKTRHMLPNGFRRS*FTMLKSWKS**CKTGS-TAQRSLMVSLR 345 +RF+G MP IGYG+NKKTR+ +PNG + + + + A+ + VS R Sbjct: 41 RRFRGTISMPKIGYGNNKKTRYCMPNGLKAFLVRNVSDVELLLMHNKTYAAEIAGNVSAR 100 Query: 346 RSGS*FVERAQQLSIRVTNAAARLRSQE 429 + VE+A+ L ++VTNA A++RSQE Sbjct: 101 KRVE-IVEKARALGVKVTNAGAKVRSQE 127 Score = 54.8 bits (126), Expect = 6e-09 Identities = 23/35 (65%), Positives = 29/35 (82%) Frame = +2 Query: 65 IVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVR 169 I+KKRTK F RHQSDR+ ++ +WRKPRGID+ VR Sbjct: 6 IIKKRTKPFKRHQSDRFKRVGESWRKPRGIDSCVR 40 Score = 42.3 bits (95), Expect = 4e-05 Identities = 21/47 (44%), Positives = 30/47 (63%) Frame = +3 Query: 240 PKWIPKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIRGKSPA 380 P + LV NV ++E+L+M N+ Y AEIA VS++KR I K+ A Sbjct: 65 PNGLKAFLVRNVSDVELLLMHNKTYAAEIAGNVSARKRVEIVEKARA 111 >SPBC6B1.10 |prp17||splicing factor Prp17|Schizosaccharomyces pombe|chr 2|||Manual Length = 558 Score = 27.5 bits (58), Expect = 1.1 Identities = 13/23 (56%), Positives = 17/23 (73%), Gaps = 2/23 (8%) Frame = -3 Query: 139 TPIPLKFV--IAIRLMPDKSLRP 77 TP+P+KFV IA+ MP +LRP Sbjct: 427 TPVPIKFVADIAMHSMPRVALRP 449 >SPAC139.03 |||transcription factor, zf-fungal binuclear cluster type |Schizosaccharomyces pombe|chr 1|||Manual Length = 625 Score = 27.1 bits (57), Expect = 1.5 Identities = 17/48 (35%), Positives = 22/48 (45%), Gaps = 3/48 (6%) Frame = -1 Query: 405 RIRHSDAELLGSFHESASAS---SKRHHERSLRSTSCFASSRFPAL*H 271 R+ D EL F E + S HER +R + S+RFP L H Sbjct: 424 RLLELDRELSNFFKELIEVNIPTSNTMHERHIRLLVSYLSNRFPILLH 471 >SPAC1250.04c |atl1||alkyltransferase-like protein Atl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 108 Score = 27.1 bits (57), Expect = 1.5 Identities = 14/32 (43%), Positives = 20/32 (62%) Frame = +1 Query: 13 VSKRNIQDGYKTCLQADNRQKEDEEIYQASIG 108 +SKR+I G + Q D ++E EIYQ S+G Sbjct: 66 ISKRDISAGEQR--QKDRLEEEGVEIYQTSLG 95 >SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosaccharomyces pombe|chr 2|||Manual Length = 479 Score = 25.4 bits (53), Expect = 4.5 Identities = 17/57 (29%), Positives = 29/57 (50%), Gaps = 1/57 (1%) Frame = -2 Query: 446 NYIY-SFSWERRRAAAFVTLMLSCWALSTNQLPLLRRDTMSDLCAVLPVLHHQDFQL 279 N++Y F+W+ + AA + S S QLP L + + L V++ QDF++ Sbjct: 217 NFVYVMFTWDNKAAALYQAFFRSGLTSSQIQLPNL-WNFFATLLVFGVVIYLQDFRV 272 >SPBC336.10c |tif512||translation initiation factor|Schizosaccharomyces pombe|chr 2|||Manual Length = 157 Score = 25.0 bits (52), Expect = 5.9 Identities = 9/29 (31%), Positives = 15/29 (51%) Frame = +1 Query: 25 NIQDGYKTCLQADNRQKEDEEIYQASIGS 111 NI DGY + D K+D + + +G+ Sbjct: 95 NIDDGYLNLMTTDGTTKDDVRLPEGELGN 123 >SPAC26H5.10c |tif51||translation initiation factor eIF5A|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 25.0 bits (52), Expect = 5.9 Identities = 9/29 (31%), Positives = 15/29 (51%) Frame = +1 Query: 25 NIQDGYKTCLQADNRQKEDEEIYQASIGS 111 NI DGY + D K+D + + +G+ Sbjct: 95 NIDDGYLNLMTTDGTTKDDVRLPEGELGN 123 >SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 372 Score = 24.6 bits (51), Expect = 7.9 Identities = 15/60 (25%), Positives = 26/60 (43%) Frame = +2 Query: 5 TVAFLKETYKMAIRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRSGSRV 184 ++ L +AIR R K +T+RFI R + + + I+N ++G V Sbjct: 246 SIIMLITVQALAIRKFIRTYRTKSKTRRFIEDLPSRTISREGFYSEEEEIENSTQNGELV 305 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,887,366 Number of Sequences: 5004 Number of extensions: 37493 Number of successful extensions: 119 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 112 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 117 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 184476110 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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