Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30412.Seq
(477 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g18100.1 68417.m02692 60S ribosomal protein L32 (RPL32A) ribo... 69 1e-12
At5g46430.2 68418.m05716 60S ribosomal protein L32 (RPL32B) 66 2e-11
At5g46430.1 68418.m05715 60S ribosomal protein L32 (RPL32B) 66 2e-11
At5g41840.1 68418.m05095 F-box family protein contains F-box dom... 33 0.13
At1g79930.1 68414.m09340 heat shock protein, putative contains P... 29 1.6
At5g07810.1 68418.m00895 SNF2 domain-containing protein / helica... 28 2.8
At4g01720.1 68417.m00223 WRKY family transcription factor simila... 28 2.8
At3g13570.1 68416.m01707 SC35-like splicing factor, 30a kD (SCL3... 28 3.8
At1g26570.1 68414.m03237 UDP-glucose 6-dehydrogenase, putative s... 28 3.8
At4g07670.1 68417.m01203 protease-associated (PA) domain-contain... 27 5.0
At1g22880.1 68414.m02856 glycosyl hydrolase family 9 protein sim... 27 5.0
At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam d... 27 6.6
At3g07210.1 68416.m00860 expressed protein predicted using genef... 27 6.6
At5g44490.1 68418.m05451 F-box family protein contains F-box dom... 27 8.7
At4g02020.1 68417.m00272 zeste-like protein 1 (EZA1) identical t... 27 8.7
At3g59160.1 68416.m06596 F-box family protein contains F-box dom... 27 8.7
>At4g18100.1 68417.m02692 60S ribosomal protein L32 (RPL32A)
ribosomal protein L32, human, PIR1:R5HU32
Length = 133
Score = 69.3 bits (162), Expect = 1e-12
Identities = 41/90 (45%), Positives = 56/90 (62%), Gaps = 1/90 (1%)
Frame = +1
Query: 169 QRFKGQYLMPNIGYGSNKKTRHMLPNGFRRS*FTMLKSWKS**CKTGS-TAQRSLMVSLR 345
++FKG LMPN+GYGS+KKTRH LPNGF++ + + A+ + VS +
Sbjct: 45 RKFKGVTLMPNVGYGSDKKTRHYLPNGFKKFVVHNTSELELLMMHNRTYCAEIAHNVSTK 104
Query: 346 RSGS*FVERAQQLSIRVTNAAARLRSQENE 435
+ + VERA QL + VTN ARLRSQE+E
Sbjct: 105 KRKA-IVERASQLDVVVTNRLARLRSQEDE 133
Score = 62.9 bits (146), Expect = 1e-10
Identities = 28/43 (65%), Positives = 31/43 (72%)
Frame = +3
Query: 234 YAPKWIPKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLI 362
Y P K +VHN ELE+LMM NR YCAEIAH VS+KKRK I
Sbjct: 67 YLPNGFKKFVVHNTSELELLMMHNRTYCAEIAHNVSTKKRKAI 109
Score = 52.8 bits (121), Expect = 1e-07
Identities = 24/45 (53%), Positives = 34/45 (75%)
Frame = +2
Query: 35 MAIRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVR 169
MA+ P+ +VKKR+ +FIR QSDR +K +WR+P+GID+RVR
Sbjct: 1 MAV-PLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVR 44
>At5g46430.2 68418.m05716 60S ribosomal protein L32 (RPL32B)
Length = 133
Score = 65.7 bits (153), Expect = 2e-11
Identities = 38/90 (42%), Positives = 55/90 (61%), Gaps = 1/90 (1%)
Frame = +1
Query: 169 QRFKGQYLMPNIGYGSNKKTRHMLPNGFRRS*FTMLKSWKS**CKTGS-TAQRSLMVSLR 345
++FKG LMPN+GYGS+KKTRH LPNGF++ + + A+ + +S +
Sbjct: 45 RKFKGVTLMPNVGYGSDKKTRHYLPNGFKKFIVHNTSELELLMMHNRTYCAEIAHNISTK 104
Query: 346 RSGS*FVERAQQLSIRVTNAAARLRSQENE 435
+ + VERA QL + V+N RLRSQE+E
Sbjct: 105 KRKA-IVERASQLDVVVSNKLGRLRSQEDE 133
Score = 62.9 bits (146), Expect = 1e-10
Identities = 27/43 (62%), Positives = 31/43 (72%)
Frame = +3
Query: 234 YAPKWIPKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLI 362
Y P K +VHN ELE+LMM NR YCAEIAH +S+KKRK I
Sbjct: 67 YLPNGFKKFIVHNTSELELLMMHNRTYCAEIAHNISTKKRKAI 109
Score = 52.8 bits (121), Expect = 1e-07
Identities = 24/45 (53%), Positives = 34/45 (75%)
Frame = +2
Query: 35 MAIRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVR 169
MA+ P+ +VKKR+ +FIR QSDR +K +WR+P+GID+RVR
Sbjct: 1 MAV-PLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVR 44
>At5g46430.1 68418.m05715 60S ribosomal protein L32 (RPL32B)
Length = 133
Score = 65.7 bits (153), Expect = 2e-11
Identities = 38/90 (42%), Positives = 55/90 (61%), Gaps = 1/90 (1%)
Frame = +1
Query: 169 QRFKGQYLMPNIGYGSNKKTRHMLPNGFRRS*FTMLKSWKS**CKTGS-TAQRSLMVSLR 345
++FKG LMPN+GYGS+KKTRH LPNGF++ + + A+ + +S +
Sbjct: 45 RKFKGVTLMPNVGYGSDKKTRHYLPNGFKKFIVHNTSELELLMMHNRTYCAEIAHNISTK 104
Query: 346 RSGS*FVERAQQLSIRVTNAAARLRSQENE 435
+ + VERA QL + V+N RLRSQE+E
Sbjct: 105 KRKA-IVERASQLDVVVSNKLGRLRSQEDE 133
Score = 62.9 bits (146), Expect = 1e-10
Identities = 27/43 (62%), Positives = 31/43 (72%)
Frame = +3
Query: 234 YAPKWIPKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLI 362
Y P K +VHN ELE+LMM NR YCAEIAH +S+KKRK I
Sbjct: 67 YLPNGFKKFIVHNTSELELLMMHNRTYCAEIAHNISTKKRKAI 109
Score = 52.8 bits (121), Expect = 1e-07
Identities = 24/45 (53%), Positives = 34/45 (75%)
Frame = +2
Query: 35 MAIRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVR 169
MA+ P+ +VKKR+ +FIR QSDR +K +WR+P+GID+RVR
Sbjct: 1 MAV-PLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVR 44
>At5g41840.1 68418.m05095 F-box family protein contains F-box domain
Pfam:PF00646
Length = 540
Score = 32.7 bits (71), Expect = 0.13
Identities = 12/31 (38%), Positives = 20/31 (64%)
Frame = +3
Query: 225 DPSYAPKWIPKVLVHNVKELEILMMQNRKYC 317
D S+ +WIPKVL V ++++ + +R YC
Sbjct: 110 DQSWVLEWIPKVLKRGVLDIDLHITSSRNYC 140
>At1g79930.1 68414.m09340 heat shock protein, putative contains Pfam
profile: PF00012 Heat shock hsp70 proteins; similar to
heat-shock proteins GB:CAA94389, GB:AAD55461
[Arabidopsis thaliana]
Length = 831
Score = 29.1 bits (62), Expect = 1.6
Identities = 27/90 (30%), Positives = 46/90 (51%), Gaps = 4/90 (4%)
Frame = +3
Query: 162 ESAAVQGSILDAQHWLRFQQEDPSYAPKW-IPKVLVHNVK-ELEILMMQNRKYCAEIAHG 335
E V ++A+ WLR +Q+ PK+ P +L +VK + E L K+C I
Sbjct: 732 EKQKVLNECVEAEAWLRGKQQQQDTLPKYATPALLSADVKSKAEAL----DKFCRPI--- 784
Query: 336 VSSKKRKLIRGKSPAAQ--HQSDECGRSPP 419
+K + + + ++P A+ Q+DE G+S P
Sbjct: 785 -MTKPKPVAKAEAPQAKGGEQADE-GKSEP 812
>At5g07810.1 68418.m00895 SNF2 domain-containing protein / helicase
domain-containing protein / HNH endonuclease
domain-containing protein similar to HepA-related
protein HARP [Homo sapiens] GI:6693791; contains Pfam
profiles PF00271: Helicase conserved C-terminal domain,
PF00176: SNF2 family N-terminal domain, PF01844: HNH
endonuclease
Length = 1190
Score = 28.3 bits (60), Expect = 2.8
Identities = 13/31 (41%), Positives = 21/31 (67%)
Frame = +3
Query: 201 HWLRFQQEDPSYAPKWIPKVLVHNVKELEIL 293
H + Q++P+Y P+W PKV+V + K L+ L
Sbjct: 278 HLVFGHQDNPAYLPRW-PKVVVISYKMLQHL 307
>At4g01720.1 68417.m00223 WRKY family transcription factor similar
to wild oat DNA-binding protein ABF2, GenBank accession
number Z48431
Length = 489
Score = 28.3 bits (60), Expect = 2.8
Identities = 13/43 (30%), Positives = 21/43 (48%)
Frame = -1
Query: 411 SGRIRHSDAELLGSFHESASASSKRHHERSLRSTSCFASSRFP 283
SG H D++ H+ + +RHHE S++ FA+ P
Sbjct: 16 SGEFLHGDSD--SKDHQPNESPVERHHESSIKEVDFFAAKSQP 56
>At3g13570.1 68416.m01707 SC35-like splicing factor, 30a kD (SCL30a)
almost identical to SC35-like splicing factor SCL30a
GI:9843661 from [Arabidopsis thaliana]; contains Pfam
profile PF00076: RNA recognition motif. (a.k.a. RRM,
RBD, or RNP domain)
Length = 262
Score = 27.9 bits (59), Expect = 3.8
Identities = 15/42 (35%), Positives = 20/42 (47%)
Frame = +2
Query: 53 YRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRSGS 178
Y K+ R + Q RY+K + R P +RVRSGS
Sbjct: 164 YNSPPAKRHQSRSVSPQDRRYEKERSYSRSPPHNGSRVRSGS 205
>At1g26570.1 68414.m03237 UDP-glucose 6-dehydrogenase, putative
strong similarity to SP|Q96558 UDP-glucose
6-dehydrogenase (EC 1.1.1.22) (UDP-Glc dehydrogenase)
(UDP-GlcDH) (UDPGDH) {Glycine max}; contains Pfam
profiles PF03721: UDP-glucose/GDP-mannose dehydrogenase
family NAD binding domain, PF00984:
UDP-glucose/GDP-mannose dehydrogenase family central
domain, PF03720: UDP-glucose/GDP-mannose dehydrogenase
family UDP binding domain
Length = 481
Score = 27.9 bits (59), Expect = 3.8
Identities = 16/47 (34%), Positives = 25/47 (53%), Gaps = 2/47 (4%)
Frame = +3
Query: 249 IPKVLVHNVKELEILMMQNRKYCAE--IAHGVSSKKRKLIRGKSPAA 383
I K+L HN K +E ++ N ++ AE + + R LI G+ AA
Sbjct: 137 IEKILTHNSKGIEFQILSNPEFLAEGTAIKDLYNPDRVLIGGRDTAA 183
>At4g07670.1 68417.m01203 protease-associated (PA) domain-containing
protein similar to PF02225: PA domain; similar to
N-acetylated-alpha-linked acidic dipeptidase II
(NAALADase II) (SP:Q9Y3Q) {Homo sapiens};similar to
Glutamate carboxypeptidase II (Membrane glutamate
carboxypeptidase) (mGCP) (N-acetylated-alpha-linked
acidic dipeptidase I) (NAALADase I)
(Pteroylpoly-gamma-glutamate
carboxypeptidase)(Folylpoly-gamma-glutamate
carboxypeptidase) (FGCP) (Folate hydrolase 1)
(Prostate-specific membrane antigen homolog) (SP|O77564)
{Sus scrofa}
Length = 280
Score = 27.5 bits (58), Expect = 5.0
Identities = 11/37 (29%), Positives = 22/37 (59%)
Frame = +2
Query: 38 AIRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPR 148
A+ P ++ + +K +++H + R DKL++ KPR
Sbjct: 194 AVDPNSGTAVLMEASKSYLQHIAQRLDKLQKRGWKPR 230
>At1g22880.1 68414.m02856 glycosyl hydrolase family 9 protein
similar to GB:AAB65156 and GB:AAA96135
Length = 484
Score = 27.5 bits (58), Expect = 5.0
Identities = 12/42 (28%), Positives = 20/42 (47%)
Frame = -2
Query: 431 FSWERRRAAAFVTLMLSCWALSTNQLPLLRRDTMSDLCAVLP 306
FSW+ + A A+V L N L ++ + +C +LP
Sbjct: 268 FSWDNKYAGAYVLLSRRAVLNKDNNFELYKQAAENFMCKILP 309
>At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam domain
PF04931: DNA polymerase V
Length = 1306
Score = 27.1 bits (57), Expect = 6.6
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = +3
Query: 255 KVLVHNVKELEILMMQNRKYCAEIAHGVSSKK 350
K LV N+ E ++ Q RK+C I VSS K
Sbjct: 1242 KELVGNMPEAKVRRAQVRKFCGRIFQMVSSLK 1273
>At3g07210.1 68416.m00860 expressed protein predicted using
genefinder
Length = 547
Score = 27.1 bits (57), Expect = 6.6
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = -1
Query: 345 SKRHHERSLRSTSCFASSRF 286
S+ HHER RS SC +SSR+
Sbjct: 451 SEFHHERVGRSQSCVSSSRW 470
>At5g44490.1 68418.m05451 F-box family protein contains F-box domain
Pfam:PF00646
Length = 455
Score = 26.6 bits (56), Expect = 8.7
Identities = 10/37 (27%), Positives = 20/37 (54%)
Frame = +3
Query: 216 QQEDPSYAPKWIPKVLVHNVKELEILMMQNRKYCAEI 326
+ ++ S +WI V H +K L++ + +K C E+
Sbjct: 102 ENDNKSCVTRWIGFVATHKLKHLDVECLLWKKKCLEV 138
>At4g02020.1 68417.m00272 zeste-like protein 1 (EZA1) identical to
enhancer of zeste-like protein 1(EZA1) (GI:4185507)
[Arabidopsis thaliana]; similar to polycomb group
[Arabidopsis thaliana] GI:1903019 (curly leaf); contains
Pfam profile PF00856: SET domain
Length = 856
Score = 26.6 bits (56), Expect = 8.7
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = +1
Query: 22 RNIQDGYKTCLQADNRQKEDE 84
RN+ G KTCL N +E+E
Sbjct: 517 RNLLSGLKTCLDVSNYMRENE 537
>At3g59160.1 68416.m06596 F-box family protein contains F-box domain
Pfam:PF00646
Length = 464
Score = 26.6 bits (56), Expect = 8.7
Identities = 11/27 (40%), Positives = 15/27 (55%)
Frame = +3
Query: 225 DPSYAPKWIPKVLVHNVKELEILMMQN 305
DP WI KVLV V +L +++ N
Sbjct: 127 DPDCVAPWIHKVLVRGVSDLHLVIDMN 153
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,971,292
Number of Sequences: 28952
Number of extensions: 200721
Number of successful extensions: 707
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 687
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 707
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 821630280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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