BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30377.Seq (651 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g77140.1 68414.m08986 vacuolar protein sorting protein 45, pu... 66 2e-11 At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putat... 33 0.12 At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE... 33 0.16 At3g15060.1 68416.m01905 Ras-related GTP-binding family protein ... 28 4.7 At5g09860.1 68418.m01140 nuclear matrix protein-related low simi... 28 6.2 At4g29060.1 68417.m04157 elongation factor Ts family protein sim... 28 6.2 At3g11900.1 68416.m01459 amino acid transporter family protein l... 27 8.2 >At1g77140.1 68414.m08986 vacuolar protein sorting protein 45, putative / VPS45p, putative identical to vacuolar protein sorting homolog VPS45p [Arabidopsis thaliana] gi|2921406|gb|AAC39472 Length = 569 Score = 66.1 bits (154), Expect = 2e-11 Identities = 28/69 (40%), Positives = 45/69 (65%) Frame = +3 Query: 255 HMXCIVFIRPTSENIALLSRELRDPKYGVYFIYFSNVVSXADIKTLAECDXXXAVRXVQE 434 H+ + FIRPTS+NI L +L +P++G Y ++FSN++ I LA+ D V+ VQE Sbjct: 66 HLKAVYFIRPTSDNIQKLRYQLANPRFGEYHLFFSNLLKDTQIHILADSDEQEVVQQVQE 125 Query: 435 VFADYLAVD 461 +AD+++ D Sbjct: 126 YYADFVSGD 134 Score = 60.9 bits (141), Expect = 7e-10 Identities = 31/57 (54%), Positives = 42/57 (73%) Frame = +1 Query: 61 MNVIQAVKMYITKMXXXSGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDS 231 M ++ +V+ YI +M GMKV+++D ET S VS+VYSQSE+LQKEV+L E IDS Sbjct: 1 MVLVTSVRDYINRMLQDIS-GMKVLILDSETVSNVSIVYSQSELLQKEVFLVEMIDS 56 >At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putative similar to cytokinesis-related Sec1 protein KEULE [Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains Pfam domain, PF00995: Sec1 family Length = 662 Score = 33.5 bits (73), Expect = 0.12 Identities = 18/71 (25%), Positives = 38/71 (53%), Gaps = 3/71 (4%) Frame = +3 Query: 258 MXCIVFIRPTSENIALLSREL--RDPKYGVYFIYFSNVVSXADIKTL-AECDXXXAVRXV 428 M I FI+PT EN+ ++ + P Y F++FS+ VS + + + + + + Sbjct: 86 MEVIYFIQPTEENVTAFLSDMTGKSPLYKKAFVFFSSPVSRSLVNLIKKDMRAMKRIGGL 145 Query: 429 QEVFADYLAVD 461 +E+ +Y+++D Sbjct: 146 KEMNLEYISMD 156 Score = 31.9 bits (69), Expect = 0.38 Identities = 16/36 (44%), Positives = 21/36 (58%) Frame = +1 Query: 127 KVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSH 234 KV++MDK T I+S SEI Q+ + L E I H Sbjct: 44 KVLVMDKFTVKIMSSACKMSEITQEGISLVEVITKH 79 >At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE) similar to cytokinesis-related Sec1 protein KEULE [Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains Pfam domain, PF00995: Sec1 family Length = 666 Score = 33.1 bits (72), Expect = 0.16 Identities = 18/71 (25%), Positives = 37/71 (52%), Gaps = 3/71 (4%) Frame = +3 Query: 258 MXCIVFIRPTSENIALLSREL--RDPKYGVYFIYFSNVVSXADIKTL-AECDXXXAVRXV 428 M I FI+PT EN+ + ++ + P Y F++FS+ VS + + + + + Sbjct: 86 MDAIYFIQPTKENVIMFLSDMSGKSPLYKKAFVFFSSPVSKELVGHIKKDSSVLPRIGAL 145 Query: 429 QEVFADYLAVD 461 +E+ ++ A+D Sbjct: 146 REMNLEFFAID 156 >At3g15060.1 68416.m01905 Ras-related GTP-binding family protein similar to GTP-binding protein GI:303742 from [Pisum sativum]; contains Pfam profile: PF00071 ras family Length = 217 Score = 28.3 bits (60), Expect = 4.7 Identities = 13/37 (35%), Positives = 22/37 (59%) Frame = -2 Query: 452 QVVSKNFLDLSDSXXXITFGQSFDVCXRNYITEIYKV 342 +V SK LD+ D + GQS +V ++ ++E+ KV Sbjct: 176 RVASKKALDIGDDHTTLPKGQSINVGSKDDVSEVKKV 212 >At5g09860.1 68418.m01140 nuclear matrix protein-related low similarity to nuclear matrix protein p84 [Homo sapiens] GI:550058 Length = 599 Score = 27.9 bits (59), Expect = 6.2 Identities = 11/27 (40%), Positives = 14/27 (51%) Frame = -1 Query: 561 EGARADLEKLVGDAVGSTPTVEASNNI 481 EG R D E + DA+ TPT E + Sbjct: 536 EGGRGDAETMESDAIADTPTPEEQQRL 562 >At4g29060.1 68417.m04157 elongation factor Ts family protein similar to SP|P35019 Elongation factor Ts (EF-Ts) {Galdieria sulphuraria}; contains Pfam profiles PF00627: UBA/TS-N domain, PF00889: Elongation factor TS, PF00575: S1 RNA binding domain Length = 953 Score = 27.9 bits (59), Expect = 6.2 Identities = 12/30 (40%), Positives = 16/30 (53%) Frame = -1 Query: 513 STPTVEASNNIEREQVXCPQPGSQQKLPGP 424 S+P AS +E+EQV P + K P P Sbjct: 468 SSPEALASEEVEKEQVVAETPVDEVKTPAP 497 >At3g11900.1 68416.m01459 amino acid transporter family protein low similarity to proton/amino acid transporter 1 [Mus musculus] GI:21908024; belongs to INTERPRO:IPR002422 amino acid/polyamine transporter, family II Length = 432 Score = 27.5 bits (58), Expect = 8.2 Identities = 15/41 (36%), Positives = 24/41 (58%) Frame = -2 Query: 179 LYTILTMLVVSLSINITFIPGPLSXXILVMYILTACITFIL 57 ++T T+LVV L+ + +PG + LV L A I+F+L Sbjct: 349 IFTTRTLLVVGLAAIASLVPGFGTFASLVGSTLCALISFVL 389 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,017,812 Number of Sequences: 28952 Number of extensions: 218915 Number of successful extensions: 451 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 444 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 451 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1354097952 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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