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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30377.Seq
         (651 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g77140.1 68414.m08986 vacuolar protein sorting protein 45, pu...    66   2e-11
At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putat...    33   0.12 
At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE...    33   0.16 
At3g15060.1 68416.m01905 Ras-related GTP-binding family protein ...    28   4.7  
At5g09860.1 68418.m01140 nuclear matrix protein-related low simi...    28   6.2  
At4g29060.1 68417.m04157 elongation factor Ts family protein sim...    28   6.2  
At3g11900.1 68416.m01459 amino acid transporter family protein l...    27   8.2  

>At1g77140.1 68414.m08986 vacuolar protein sorting protein 45,
           putative / VPS45p, putative identical to vacuolar
           protein sorting homolog VPS45p [Arabidopsis thaliana]
           gi|2921406|gb|AAC39472
          Length = 569

 Score = 66.1 bits (154), Expect = 2e-11
 Identities = 28/69 (40%), Positives = 45/69 (65%)
 Frame = +3

Query: 255 HMXCIVFIRPTSENIALLSRELRDPKYGVYFIYFSNVVSXADIKTLAECDXXXAVRXVQE 434
           H+  + FIRPTS+NI  L  +L +P++G Y ++FSN++    I  LA+ D    V+ VQE
Sbjct: 66  HLKAVYFIRPTSDNIQKLRYQLANPRFGEYHLFFSNLLKDTQIHILADSDEQEVVQQVQE 125

Query: 435 VFADYLAVD 461
            +AD+++ D
Sbjct: 126 YYADFVSGD 134



 Score = 60.9 bits (141), Expect = 7e-10
 Identities = 31/57 (54%), Positives = 42/57 (73%)
 Frame = +1

Query: 61  MNVIQAVKMYITKMXXXSGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDS 231
           M ++ +V+ YI +M      GMKV+++D ET S VS+VYSQSE+LQKEV+L E IDS
Sbjct: 1   MVLVTSVRDYINRMLQDIS-GMKVLILDSETVSNVSIVYSQSELLQKEVFLVEMIDS 56


>At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putative
           similar to cytokinesis-related Sec1 protein KEULE
           [Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
           Pfam domain, PF00995: Sec1 family
          Length = 662

 Score = 33.5 bits (73), Expect = 0.12
 Identities = 18/71 (25%), Positives = 38/71 (53%), Gaps = 3/71 (4%)
 Frame = +3

Query: 258 MXCIVFIRPTSENIALLSREL--RDPKYGVYFIYFSNVVSXADIKTL-AECDXXXAVRXV 428
           M  I FI+PT EN+     ++  + P Y   F++FS+ VS + +  +  +      +  +
Sbjct: 86  MEVIYFIQPTEENVTAFLSDMTGKSPLYKKAFVFFSSPVSRSLVNLIKKDMRAMKRIGGL 145

Query: 429 QEVFADYLAVD 461
           +E+  +Y+++D
Sbjct: 146 KEMNLEYISMD 156



 Score = 31.9 bits (69), Expect = 0.38
 Identities = 16/36 (44%), Positives = 21/36 (58%)
 Frame = +1

Query: 127 KVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSH 234
           KV++MDK T  I+S     SEI Q+ + L E I  H
Sbjct: 44  KVLVMDKFTVKIMSSACKMSEITQEGISLVEVITKH 79


>At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE)
           similar to cytokinesis-related Sec1 protein KEULE
           [Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
           Pfam domain, PF00995: Sec1 family
          Length = 666

 Score = 33.1 bits (72), Expect = 0.16
 Identities = 18/71 (25%), Positives = 37/71 (52%), Gaps = 3/71 (4%)
 Frame = +3

Query: 258 MXCIVFIRPTSENIALLSREL--RDPKYGVYFIYFSNVVSXADIKTL-AECDXXXAVRXV 428
           M  I FI+PT EN+ +   ++  + P Y   F++FS+ VS   +  +  +      +  +
Sbjct: 86  MDAIYFIQPTKENVIMFLSDMSGKSPLYKKAFVFFSSPVSKELVGHIKKDSSVLPRIGAL 145

Query: 429 QEVFADYLAVD 461
           +E+  ++ A+D
Sbjct: 146 REMNLEFFAID 156


>At3g15060.1 68416.m01905 Ras-related GTP-binding family protein
           similar to GTP-binding protein GI:303742 from [Pisum
           sativum]; contains Pfam profile: PF00071 ras family
          Length = 217

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 13/37 (35%), Positives = 22/37 (59%)
 Frame = -2

Query: 452 QVVSKNFLDLSDSXXXITFGQSFDVCXRNYITEIYKV 342
           +V SK  LD+ D    +  GQS +V  ++ ++E+ KV
Sbjct: 176 RVASKKALDIGDDHTTLPKGQSINVGSKDDVSEVKKV 212


>At5g09860.1 68418.m01140 nuclear matrix protein-related low
           similarity to nuclear matrix protein p84 [Homo sapiens]
           GI:550058
          Length = 599

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 11/27 (40%), Positives = 14/27 (51%)
 Frame = -1

Query: 561 EGARADLEKLVGDAVGSTPTVEASNNI 481
           EG R D E +  DA+  TPT E    +
Sbjct: 536 EGGRGDAETMESDAIADTPTPEEQQRL 562


>At4g29060.1 68417.m04157 elongation factor Ts family protein
           similar to SP|P35019 Elongation factor Ts (EF-Ts)
           {Galdieria sulphuraria}; contains Pfam profiles PF00627:
           UBA/TS-N domain, PF00889: Elongation factor TS, PF00575:
           S1 RNA binding domain
          Length = 953

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 12/30 (40%), Positives = 16/30 (53%)
 Frame = -1

Query: 513 STPTVEASNNIEREQVXCPQPGSQQKLPGP 424
           S+P   AS  +E+EQV    P  + K P P
Sbjct: 468 SSPEALASEEVEKEQVVAETPVDEVKTPAP 497


>At3g11900.1 68416.m01459 amino acid transporter family protein low
           similarity to proton/amino acid transporter 1 [Mus
           musculus] GI:21908024; belongs to INTERPRO:IPR002422
           amino acid/polyamine transporter, family II
          Length = 432

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 15/41 (36%), Positives = 24/41 (58%)
 Frame = -2

Query: 179 LYTILTMLVVSLSINITFIPGPLSXXILVMYILTACITFIL 57
           ++T  T+LVV L+   + +PG  +   LV   L A I+F+L
Sbjct: 349 IFTTRTLLVVGLAAIASLVPGFGTFASLVGSTLCALISFVL 389


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,017,812
Number of Sequences: 28952
Number of extensions: 218915
Number of successful extensions: 451
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 444
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 451
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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