BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30367.Seq (815 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomy... 142 4e-35 SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 133 2e-32 SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 88 2e-18 SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharo... 60 5e-10 SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2||... 38 0.002 SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 35 0.012 SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1||... 35 0.012 SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|... 34 0.028 SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr 1|||... 28 1.4 SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr 1||... 28 1.4 SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 27 3.2 SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|ch... 27 4.2 SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ... 26 5.6 SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 26 7.4 SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 26 7.4 >SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomyces pombe|chr 2|||Manual Length = 180 Score = 142 bits (345), Expect = 4e-35 Identities = 70/145 (48%), Positives = 89/145 (61%) Frame = +2 Query: 254 YKLQVGEVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDS 433 YKL++GE+VTTIPTIGFNVE V Y+N+ F VWD+GGQ IRP WR Y+ NT II+VVDS Sbjct: 35 YKLKLGEIVTTIPTIGFNVETVEYRNISFTVWDVGGQDKIRPLWRHYFQNTQGIIFVVDS 94 Query: 434 ADRDRIGISKDELVHMLRXXXXXXXXXXXXXXKQDMAGCLTVAEVHQALGLDALRDRTFQ 613 DR+RI + +EL ML KQD+ + AE+ LGL +LR R + Sbjct: 95 NDRERISEAHEELQRMLNEDELRDALLLVFANKQDLPNAMNAAEITDKLGLHSLRHRQWY 154 Query: 614 IFKTSAVRGXGLDQAMDWALETRFK 688 I T A G GL + ++W L T K Sbjct: 155 IQATCATSGDGLYEGLEW-LSTNLK 178 Score = 43.6 bits (98), Expect = 3e-05 Identities = 20/30 (66%), Positives = 23/30 (76%) Frame = +3 Query: 177 FRGLLGAREMRILILGLDGAGKTTILINCK 266 F+ L G REMRIL++GLD AGKTTIL K Sbjct: 9 FQSLFGKREMRILMVGLDAAGKTTILYKLK 38 >SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 133 bits (322), Expect = 2e-32 Identities = 62/138 (44%), Positives = 86/138 (62%) Frame = +2 Query: 254 YKLQVGEVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDS 433 YKL++ + V TIPT+GFNVE VTYKN+KF VWD+GGQ IRP WR Y+ T +I+VVDS Sbjct: 39 YKLKLNQSVVTIPTVGFNVETVTYKNIKFNVWDVGGQDKIRPLWRHYFTGTKGLIFVVDS 98 Query: 434 ADRDRIGISKDELVHMLRXXXXXXXXXXXXXXKQDMAGCLTVAEVHQALGLDALRDRTFQ 613 AD +RI ++ EL ++ KQD+ G L+ A++ L LD L+DR + Sbjct: 99 ADSNRISEARQELHRIISDREMRDCLLLVLANKQDLPGALSPAQITDVLQLDKLKDRLWN 158 Query: 614 IFKTSAVRGXGLDQAMDW 667 + T A+ G GL + + W Sbjct: 159 VQPTCALTGDGLLEGLAW 176 Score = 39.5 bits (88), Expect = 6e-04 Identities = 20/35 (57%), Positives = 22/35 (62%) Frame = +3 Query: 162 GLFSYFRGLLGAREMRILILGLDGAGKTTILINCK 266 G F L +EMRIL+LGLD AGKTTIL K Sbjct: 8 GFSKPFSRLFSNKEMRILMLGLDAAGKTTILYKLK 42 >SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosaccharomyces pombe|chr 1|||Manual Length = 186 Score = 87.8 bits (208), Expect = 2e-18 Identities = 39/126 (30%), Positives = 69/126 (54%) Frame = +2 Query: 290 PTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDRIGISKDE 469 PT GF + + + L+F +WD+GGQ ++R +W+ Y+ +T+AII+VVDS D R+ ++ Sbjct: 46 PTFGFQIRTLEVEGLRFTIWDIGGQKTLRNFWKNYFESTEAIIWVVDSLDDLRLEECRNT 105 Query: 470 LVHMLRXXXXXXXXXXXXXXKQDMAGCLTVAEVHQALGLDALRDRTFQIFKTSAVRGXGL 649 L +L K D++G L+ E+ + L + + ++IF SA+ G + Sbjct: 106 LQELLVEEKLLFTSILVLANKSDVSGALSSEEISKILNISKYKSSHWRIFSVSALTGLNI 165 Query: 650 DQAMDW 667 A+ W Sbjct: 166 KDAISW 171 Score = 35.9 bits (79), Expect = 0.007 Identities = 16/22 (72%), Positives = 19/22 (86%) Frame = +3 Query: 189 LGAREMRILILGLDGAGKTTIL 254 L RE+R+L+LGLD AGKTTIL Sbjct: 12 LKEREVRVLLLGLDNAGKTTIL 33 >SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharomyces pombe|chr 2|||Manual Length = 190 Score = 59.7 bits (138), Expect = 5e-10 Identities = 32/99 (32%), Positives = 48/99 (48%) Frame = +2 Query: 290 PTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDRIGISKDE 469 PT+ E++ N++F +DLGG R WR Y+ + I+Y+VD D +R+ SK E Sbjct: 50 PTLHPTSEELAIGNVRFTTFDLGGHQQARRLWRDYFPEVNGIVYLVDCCDFERLSESKAE 109 Query: 470 LVHMLRXXXXXXXXXXXXXXKQDMAGCLTVAEVHQALGL 586 L +L K D G ++ E+ ALGL Sbjct: 110 LDALLAMEELARVPFLILGNKIDAPGAISEDELKAALGL 148 Score = 28.7 bits (61), Expect = 1.0 Identities = 13/26 (50%), Positives = 18/26 (69%) Frame = +3 Query: 198 REMRILILGLDGAGKTTILINCKSVR 275 + ++L LGLD AGKTT+L K+ R Sbjct: 19 KHAKMLFLGLDNAGKTTLLHMLKNDR 44 >SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 203 Score = 37.9 bits (84), Expect = 0.002 Identities = 21/57 (36%), Positives = 27/57 (47%), Gaps = 4/57 (7%) Frame = +2 Query: 287 IPTIGFNVEQVTY----KNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRD 445 I TIG + + T+ K +K Q+WD GQ R YY II V D D+D Sbjct: 38 ISTIGVDFKIRTFELEGKTVKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQD 94 >SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 35.1 bits (77), Expect = 0.012 Identities = 16/52 (30%), Positives = 30/52 (57%) Frame = +2 Query: 293 TIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDR 448 T+G + T +L+ +++D+GGQ + R W + N ++II+ V D D+ Sbjct: 179 TLGISEISFTLDHLQIRMFDVGGQRTERRKWIYCFENVNSIIFCVSLNDYDK 230 >SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 35.1 bits (77), Expect = 0.012 Identities = 20/68 (29%), Positives = 31/68 (45%), Gaps = 1/68 (1%) Frame = +2 Query: 245 DNSYKLQVG-EVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIY 421 D+ VG + + I ++G +Q K +K Q+WD GQ R R YY + Sbjct: 35 DDQVSHTVGIDFASRIISVGMGNQQ---KRIKLQIWDTAGQEKFRSVARNYYRGAAGAVL 91 Query: 422 VVDSADRD 445 V D ++D Sbjct: 92 VYDVTNKD 99 >SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 33.9 bits (74), Expect = 0.028 Identities = 18/57 (31%), Positives = 26/57 (45%), Gaps = 2/57 (3%) Frame = +2 Query: 278 VTTIPTIGFNVEQVTY--KNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADR 442 +TTI I F + + K +K Q+WD GQ R YY I+ + D D+ Sbjct: 39 ITTIG-IDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGILLLYDVTDK 94 >SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 211 Score = 28.3 bits (60), Expect = 1.4 Identities = 11/34 (32%), Positives = 17/34 (50%) Frame = +2 Query: 329 NLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVD 430 ++K ++WD GQ + YY N + I V D Sbjct: 63 SVKLEIWDTAGQERYKSLAPMYYRNANCAIVVYD 96 >SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr 1|||Manual Length = 214 Score = 28.3 bits (60), Expect = 1.4 Identities = 12/35 (34%), Positives = 15/35 (42%) Frame = +2 Query: 326 KNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVD 430 K +K Q+WD GQ R YY + V D Sbjct: 57 KKIKAQIWDTAGQERYRAITSAYYRGAVGALIVYD 91 >SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces pombe|chr 2|||Manual Length = 1142 Score = 27.1 bits (57), Expect = 3.2 Identities = 10/31 (32%), Positives = 19/31 (61%) Frame = -2 Query: 463 FRYSYSIPVS*VYNIYYCICVAIVTSPIRPN 371 F ++Y++ S VYN + +C + T+P + N Sbjct: 494 FDFTYNLSESEVYNTVFNLCDELYTTPCKKN 524 >SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|chr 1|||Manual Length = 198 Score = 26.6 bits (56), Expect = 4.2 Identities = 13/59 (22%), Positives = 28/59 (47%), Gaps = 2/59 (3%) Frame = +2 Query: 296 IGFNVEQVTYKN--LKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDRIGISKD 466 + F + ++ +N + F +WDLGGQ + AI+++ D + + + K+ Sbjct: 45 VNFMEKTISIRNTEITFSIWDLGGQREFVNMLPMVCNDAVAILFMFDLSRKSTLNSIKE 103 >SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 407 Score = 26.2 bits (55), Expect = 5.6 Identities = 9/21 (42%), Positives = 16/21 (76%) Frame = +3 Query: 192 GAREMRILILGLDGAGKTTIL 254 G ++++L+LG +GKTTI+ Sbjct: 71 GGNDIKVLLLGAGDSGKTTIM 91 >SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9|Schizosaccharomyces pombe|chr 1|||Manual Length = 1223 Score = 25.8 bits (54), Expect = 7.4 Identities = 13/34 (38%), Positives = 19/34 (55%) Frame = -2 Query: 295 RRYSGHDLTDLQFIRIVVFPAPSNPSIKIRISLA 194 R S H+L+ + FIR + P P++ KI LA Sbjct: 1107 RNNSSHELSLVAFIREMAMPVPNDEFKKISTILA 1140 >SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 25.8 bits (54), Expect = 7.4 Identities = 13/44 (29%), Positives = 20/44 (45%) Frame = -3 Query: 135 NLLLLIRNVYGLRLHIKDF*NCFFVLIYTWSTLNILFNNKYCTF 4 N + +R+ + R HI C ++ WS + L NN Y F Sbjct: 422 NKCIQLRDYFDYRKHI-----CIVTDLFGWSVFDFLKNNNYIPF 460 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,233,780 Number of Sequences: 5004 Number of extensions: 68065 Number of successful extensions: 186 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 179 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 186 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 398435810 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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