Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30363.Seq
(715 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g20290.1 68418.m02415 40S ribosomal protein S8 (RPS8A) riboso... 97 1e-20
At5g59240.1 68418.m07424 40S ribosomal protein S8 (RPS8B) 40S ri... 90 2e-18
At2g22830.1 68415.m02711 squalene monooxygenase, putative / squa... 31 0.76
At5g44010.1 68418.m05386 expressed protein 29 2.3
At1g73260.1 68414.m08478 trypsin and protease inhibitor family p... 29 3.1
At5g44890.1 68418.m05504 Ulp1 protease family protein contains P... 28 7.1
At4g04400.1 68417.m00634 hypothetical protein contains Pfam prof... 28 7.1
At3g26530.1 68416.m03312 Ulp1 protease family protein contains P... 28 7.1
At1g21020.1 68414.m02630 hypothetical protein similar to At3g265... 28 7.1
At1g08740.1 68414.m00971 Ulp1 protease family protein contains P... 28 7.1
>At5g20290.1 68418.m02415 40S ribosomal protein S8 (RPS8A) ribosomal
protein S8 - Zea mays, PIR:T04088
Length = 222
Score = 96.7 bits (230), Expect = 1e-20
Identities = 78/172 (45%), Positives = 93/172 (54%), Gaps = 19/172 (11%)
Frame = +1
Query: 31 MGISRDHWHKRRATGWETCAHTQEEEV*XRASRCKHQARPSANPLRSF--TWWKY*VPCA 204
MGISRD HKRRATG + Q++ R R R AN S T + V
Sbjct: 1 MGISRDSIHKRRATGGK-----QKQ---WRKKRKYEMGRQPANTKLSSNKTVRRIRVRGG 52
Query: 205 ASGHR*LLLGIGMFN-------SQTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQ 363
R L L G ++ +TR++DVVYNASNNELVRTKTLVK+AIV VDA PF+Q
Sbjct: 53 NVKWRALRLDTGNYSWGSEATTRKTRVLDVVYNASNNELVRTKTLVKSAIVQVDAAPFKQ 112
Query: 364 WYESHYTLPLGR-KKGAKLT-----EAEEAII----NKKRSQKTARKYLARQ 489
WY SHY + LGR KK A T E EEA + K+S RK +RQ
Sbjct: 113 WYLSHYGVELGRKKKSASSTKKDGEEGEEAAVAAPEEVKKSNHLLRKIASRQ 164
Score = 81.4 bits (192), Expect = 5e-16
Identities = 37/61 (60%), Positives = 46/61 (75%), Gaps = 1/61 (1%)
Frame = +2
Query: 77 GKRAPIRKKRKYXLGRPAANTRLGPQR-IHSVRSRGGNTKYRALRLDTGNFSWGSECSTR 253
GK+ RKKRKY +GR ANT+L + + +R RGGN K+RALRLDTGN+SWGSE +TR
Sbjct: 16 GKQKQWRKKRKYEMGRQPANTKLSSNKTVRRIRVRGGNVKWRALRLDTGNYSWGSEATTR 75
Query: 254 K 256
K
Sbjct: 76 K 76
Score = 56.0 bits (129), Expect = 2e-08
Identities = 22/28 (78%), Positives = 27/28 (96%)
Frame = +3
Query: 513 LEEQFHTGRLLACVASRPGQCGRADGYI 596
+E+QF +GRLLAC++SRPGQCGRADGYI
Sbjct: 173 IEDQFASGRLLACISSRPGQCGRADGYI 200
>At5g59240.1 68418.m07424 40S ribosomal protein S8 (RPS8B) 40S
ribosomal protein S8, Prunus armeniaca, EMBL:AF071889
Length = 210
Score = 89.8 bits (213), Expect = 2e-18
Identities = 63/156 (40%), Positives = 84/156 (53%), Gaps = 3/156 (1%)
Frame = +1
Query: 31 MGISRDHWHKRRATGWETCAHTQEE--EV*XRASRCKHQARPSANPLRSFTW-WKY*VPC 201
MGISRD HKRRATG + ++ E+ + + K + + +R K+
Sbjct: 1 MGISRDSIHKRRATGGKQKMWRKKRKYELGRQPANTKLSSNKTVRRIRVRGGNVKWRALR 60
Query: 202 AASGHR*LLLGIGMFNSQTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHY 381
+G+ G +TRI+DV YNASNNELVRT+TLVK+AIV VDA PF+Q Y HY
Sbjct: 61 LDTGN--FSWGSEAVTRKTRILDVAYNASNNELVRTQTLVKSAIVQVDAAPFKQGYLQHY 118
Query: 382 TLPLGRKKGAKLTEAEEAIINKKRSQKTARKYLARQ 489
+ +GRKK + EE K+S RK RQ
Sbjct: 119 GVDIGRKKKGEAVTTEEV----KKSNHVQRKLEMRQ 150
Score = 81.8 bits (193), Expect = 4e-16
Identities = 39/61 (63%), Positives = 45/61 (73%), Gaps = 1/61 (1%)
Frame = +2
Query: 77 GKRAPIRKKRKYXLGRPAANTRLGPQR-IHSVRSRGGNTKYRALRLDTGNFSWGSECSTR 253
GK+ RKKRKY LGR ANT+L + + +R RGGN K+RALRLDTGNFSWGSE TR
Sbjct: 16 GKQKMWRKKRKYELGRQPANTKLSSNKTVRRIRVRGGNVKWRALRLDTGNFSWGSEAVTR 75
Query: 254 K 256
K
Sbjct: 76 K 76
Score = 59.7 bits (138), Expect = 2e-09
Identities = 25/28 (89%), Positives = 27/28 (96%)
Frame = +3
Query: 513 LEEQFHTGRLLACVASRPGQCGRADGYI 596
LEEQF +GRLLAC+ASRPGQCGRADGYI
Sbjct: 159 LEEQFSSGRLLACIASRPGQCGRADGYI 186
>At2g22830.1 68415.m02711 squalene monooxygenase, putative /
squalene epoxidase, putative similar to SP|O65404 (SE
1,1), SP|O65402 (SE 1,2), SP|O65403 (SE 2)
Length = 585
Score = 31.1 bits (67), Expect = 0.76
Identities = 16/49 (32%), Positives = 25/49 (51%)
Frame = -3
Query: 377 CDSYHCLNGVASTTTIAFLTRVFVRTNSLLDALYTTSMIRVCELNIPIP 231
C Y L GV S+ +A L+ + R SL+ + ++ VC L +P P
Sbjct: 492 CFDYLSLGGVFSSGPVALLSGLNPRPLSLVLHFFAVAIYAVCRLMLPFP 540
>At5g44010.1 68418.m05386 expressed protein
Length = 357
Score = 29.5 bits (63), Expect = 2.3
Identities = 16/48 (33%), Positives = 27/48 (56%), Gaps = 2/48 (4%)
Frame = -2
Query: 327 ILDKGLCTHQFIVRCIIHNINDTGLRV--EHSDPQEKLPVSRRSARYL 190
I+D LC Q +V C+ + +TGL++ +H +P+E L A +L
Sbjct: 98 IIDGKLCKRQSVVSCV--SALETGLKILSKHIEPKESLHREDNDATFL 143
>At1g73260.1 68414.m08478 trypsin and protease inhibitor family
protein / Kunitz family protein similar to trypsin
inhibitor propeptide [Brassica oleracea] GI:841208;
contains Pfam profile PF00197: Trypsin and protease
inhibitor
Length = 215
Score = 29.1 bits (62), Expect = 3.1
Identities = 18/44 (40%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
Frame = +1
Query: 298 LVRTKTLVKNAI-VVVDATPFRQWYESHYTLPLGRKKGAKLTEA 426
L T L NA VVD ++ES+Y LP+ R +G LT A
Sbjct: 16 LALTAVLASNAYGAVVDIDGNAMFHESYYVLPVIRGRGGGLTLA 59
>At5g44890.1 68418.m05504 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain; similar to At1g21030, At2g29240,
At1g08740, At1g08760
Length = 1015
Score = 27.9 bits (59), Expect = 7.1
Identities = 21/77 (27%), Positives = 32/77 (41%), Gaps = 1/77 (1%)
Frame = -2
Query: 270 INDTGLRVEHSDPQEKLPVSRRSARYLVFPPRERTEWIR*GPSLVFAAGRPXSYFLFLRM 91
I GL + P + +P + + L+ PP + E+ PSL P Y + + M
Sbjct: 612 IGGEGLDDIQTSPDKPIPAAASHTQCLLPPPTDVNEFFESEPSL------PDPYVVLVDM 665
Query: 90 -GARFPAGGPSLMPVIP 43
G + G P L IP
Sbjct: 666 DGVAYNVGQPELQYTIP 682
>At4g04400.1 68417.m00634 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 735
Score = 27.9 bits (59), Expect = 7.1
Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
Frame = -2
Query: 333 NCILDKGLCTHQFIVRCIIHNINDTGL-RVEHSDPQEKLP 217
N I KG+C RCI++ I+D + +E+ P+ +P
Sbjct: 530 NMIWWKGMCEMDISSRCIVNEISDEEVHEIENEVPETAVP 569
>At3g26530.1 68416.m03312 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain; similar to At1g21020, At1g08760,
At1g08740, At2g29240, At1g35650
Length = 1015
Score = 27.9 bits (59), Expect = 7.1
Identities = 21/77 (27%), Positives = 32/77 (41%), Gaps = 1/77 (1%)
Frame = -2
Query: 270 INDTGLRVEHSDPQEKLPVSRRSARYLVFPPRERTEWIR*GPSLVFAAGRPXSYFLFLRM 91
I GL + P + +P + + L+ PP + E+ PSL P Y + + M
Sbjct: 612 IGGEGLDDIQTSPDKPIPAAASHTQCLLPPPTDVNEFFESEPSL------PDPYVVLVDM 665
Query: 90 -GARFPAGGPSLMPVIP 43
G + G P L IP
Sbjct: 666 DGVAYNVGQPELQYTIP 682
>At1g21020.1 68414.m02630 hypothetical protein similar to At3g26530,
At1g08760, At1g08740, At2g29240, At1g35650
Length = 751
Score = 27.9 bits (59), Expect = 7.1
Identities = 21/77 (27%), Positives = 32/77 (41%), Gaps = 1/77 (1%)
Frame = -2
Query: 270 INDTGLRVEHSDPQEKLPVSRRSARYLVFPPRERTEWIR*GPSLVFAAGRPXSYFLFLRM 91
I GL + P + +P + + L+ PP + E+ PSL P Y + + M
Sbjct: 612 IGGEGLDDIQTSPDKPIPAAASHTQCLLPPPTDVNEFFESEPSL------PDPYVVLVDM 665
Query: 90 -GARFPAGGPSLMPVIP 43
G + G P L IP
Sbjct: 666 DGVAYNVGQPELQYTIP 682
>At1g08740.1 68414.m00971 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain; similar to At1g21020, At3g26530,
At1g08760, At2g29240, At1g35650, At1g21030
Length = 1014
Score = 27.9 bits (59), Expect = 7.1
Identities = 21/77 (27%), Positives = 32/77 (41%), Gaps = 1/77 (1%)
Frame = -2
Query: 270 INDTGLRVEHSDPQEKLPVSRRSARYLVFPPRERTEWIR*GPSLVFAAGRPXSYFLFLRM 91
I GL + P + +P + + L+ PP + E+ PSL P Y + + M
Sbjct: 611 IGGEGLDDIQTSPDKPIPAAASHTQCLLPPPTDVNEFFESEPSL------PDPYVVLVDM 664
Query: 90 -GARFPAGGPSLMPVIP 43
G + G P L IP
Sbjct: 665 DGVAYNVGQPELQYTIP 681
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,939,927
Number of Sequences: 28952
Number of extensions: 276521
Number of successful extensions: 718
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 698
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 716
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1545769616
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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