Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30344.Seq
(577 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q9NRW7 Cluster: Vacuolar protein sorting-associated pro... 120 2e-26
UniRef50_Q54GE3 Cluster: Putative uncharacterized protein; n=1; ... 79 7e-14
UniRef50_Q18609 Cluster: Putative uncharacterized protein C44C1.... 73 6e-12
UniRef50_Q5KML3 Cluster: VpsB, putative; n=2; Basidiomycota|Rep:... 73 6e-12
UniRef50_O49048 Cluster: Vacuolar protein sorting-associated pro... 66 7e-10
UniRef50_Q0TYN0 Cluster: Putative uncharacterized protein; n=1; ... 47 8e-10
UniRef50_A0BVV2 Cluster: Chromosome undetermined scaffold_130, w... 62 7e-09
UniRef50_A3LRH9 Cluster: Vacuolar protein sorting associated pro... 62 7e-09
UniRef50_P38932 Cluster: Vacuolar protein sorting-associated pro... 61 2e-08
UniRef50_A2X4A8 Cluster: Putative uncharacterized protein; n=2; ... 60 4e-08
UniRef50_Q236N2 Cluster: Sec1 family protein; n=1; Tetrahymena t... 60 4e-08
UniRef50_Q1EPY2 Cluster: EhVps45A; n=2; Entamoeba histolytica|Re... 57 3e-07
UniRef50_Q6FS71 Cluster: Candida glabrata strain CBS138 chromoso... 56 8e-07
UniRef50_A7TEA7 Cluster: Putative uncharacterized protein; n=1; ... 56 8e-07
UniRef50_A2ESF3 Cluster: Sec1 family protein; n=2; Trichomonas v... 53 4e-06
UniRef50_Q09805 Cluster: Putative vacuolar protein sorting-assoc... 51 2e-05
UniRef50_A4RVJ6 Cluster: Predicted protein; n=2; Ostreococcus|Re... 50 5e-05
UniRef50_A1CM48 Cluster: Vacuolar protein sorting-associated pro... 47 3e-04
UniRef50_A2F2C8 Cluster: Sec1 family protein; n=1; Trichomonas v... 46 5e-04
UniRef50_Q5ABT2 Cluster: Putative uncharacterized protein VPS45;... 46 8e-04
UniRef50_Q1EPY1 Cluster: EhVps45B; n=1; Entamoeba histolytica|Re... 41 0.024
UniRef50_A2GDG9 Cluster: Sec1 family protein; n=3; Trichomonas v... 40 0.055
UniRef50_Q4Q1J8 Cluster: Vacuolar protein sorting-associated pro... 37 0.39
UniRef50_Q7P2P9 Cluster: Hypothetical Membrane Spanning Protein;... 36 0.68
UniRef50_Q4Q232 Cluster: Vacuolar protein sorting-associated pro... 36 0.68
UniRef50_A2EN46 Cluster: Sec1 family protein; n=1; Trichomonas v... 36 0.68
UniRef50_O96243 Cluster: Vacuolar protein-sorting protein VPS45,... 35 1.6
UniRef50_Q8SRN0 Cluster: Putative VACUOLAR PROTEIN SORTING-ASSOC... 34 2.8
UniRef50_UPI000069F4A8 Cluster: Hyaluronidase-1 precursor (EC 3.... 32 8.4
>UniRef50_Q9NRW7 Cluster: Vacuolar protein sorting-associated
protein 45; n=35; Eumetazoa|Rep: Vacuolar protein
sorting-associated protein 45 - Homo sapiens (Human)
Length = 570
Score = 120 bits (289), Expect = 2e-26
Identities = 73/152 (48%), Positives = 91/152 (59%), Gaps = 2/152 (1%)
Frame = +1
Query: 61 MNVIQAVKMYITKMXXESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSHAK 240
MNV+ AVK YI+KM +SGPGMKV+LMDKETT IVSMVY+QSEILQKEVYLFERIDS +
Sbjct: 1 MNVVFAVKQYISKMIEDSGPGMKVLLMDKETTGIVSMVYTQSEILQKEVYLFERIDSQNR 60
Query: 241 WDNMNT*NA*FLYVQHQRT*LCCHGN*EILNMVFTLY--XXSNVVSKADIKTLAECDDXX 414
+ FL + E+ +T+Y SNV+SK+D+K+LAE D+
Sbjct: 61 EIMKHLKAICFLRPTKENVDYIIQ---ELRRPKYTIYFIYFSNVISKSDVKSLAEADEQE 117
Query: 415 AVRXVHXVFADYPGXGXETCSLSXWLDXXQGR 510
V V + DY SL+ L QGR
Sbjct: 118 VVAEVQEFYGDYIAVNPHLFSLNI-LGCCQGR 148
>UniRef50_Q54GE3 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 563
Score = 79.0 bits (186), Expect = 7e-14
Identities = 49/144 (34%), Positives = 80/144 (55%), Gaps = 2/144 (1%)
Frame = +1
Query: 58 KMNVIQAVKMYITKMXXESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSHA 237
+M+VI +++ YI K+ GMKV+++DKET IVSMVY+QSEILQKEV+LFE+I+ +
Sbjct: 5 QMDVIASIQEYINKILTNI-QGMKVLVLDKETAGIVSMVYTQSEILQKEVFLFEKIE-NT 62
Query: 238 KWDNMNT*NA*FLYVQHQRT*LCCHGN*EILNMVFTLY--XXSNVVSKADIKTLAECDDX 411
K ++ F+ + C E+ + F Y +N +SK + +A+ D+
Sbjct: 63 KEKMLHMKGVYFIRPTQENIQSICD---ELKDPKFNKYHLFFTNTISKVSLDEIAKADEQ 119
Query: 412 XAVRXVHXVFADYPGXGXETCSLS 483
V + F D+ +T +L+
Sbjct: 120 DVVSEIQEYFGDFFAVNPDTFTLN 143
>UniRef50_Q18609 Cluster: Putative uncharacterized protein C44C1.4;
n=3; Caenorhabditis|Rep: Putative uncharacterized
protein C44C1.4 - Caenorhabditis elegans
Length = 547
Score = 72.5 bits (170), Expect = 6e-12
Identities = 46/130 (35%), Positives = 71/130 (54%), Gaps = 1/130 (0%)
Frame = +1
Query: 61 MNVIQAVKMYITKMXXESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSHAK 240
M+++Q+ + I M +G MK++LMD ETT VS ++QSE++QKEVY+F+RI++
Sbjct: 1 MDLVQSSRKLIQDMIQLAGSQMKLLLMDGETTPTVSCAFAQSEVMQKEVYIFDRIENKTS 60
Query: 241 WDNM-NT*NA*FLYVQHQRT*LCCHGN*EILNMVFTLYXXSNVVSKADIKTLAECDDXXA 417
+N+ N F+ + E + LY +N ++K D+K LAE D
Sbjct: 61 SENIKNLKCVVFVRPTPKNIERLVKELQEPRFSQYYLY-FTNTINKYDVKRLAEADKNET 119
Query: 418 VRXVHXVFAD 447
VR V VF D
Sbjct: 120 VREVQEVFLD 129
Score = 45.6 bits (103), Expect = 8e-04
Identities = 15/34 (44%), Positives = 28/34 (82%)
Frame = +3
Query: 252 EHMKCIVFIRPTSENIALLSRELRDPKYGVYFIY 353
+++KC+VF+RPT +NI L +EL++P++ Y++Y
Sbjct: 65 KNLKCVVFVRPTPKNIERLVKELQEPRFSQYYLY 98
>UniRef50_Q5KML3 Cluster: VpsB, putative; n=2; Basidiomycota|Rep:
VpsB, putative - Cryptococcus neoformans (Filobasidiella
neoformans)
Length = 686
Score = 72.5 bits (170), Expect = 6e-12
Identities = 47/132 (35%), Positives = 78/132 (59%), Gaps = 2/132 (1%)
Frame = +1
Query: 61 MNVIQAVKMYITKMXXESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSHAK 240
M+V +A++ Y+ KM + PGMKV+L+D TT IVS+V +QSE+L E+YL +RID++++
Sbjct: 1 MDVTKAIQTYLFKMINQV-PGMKVLLLDSHTTPIVSLVTTQSELLSHEIYLVDRIDNNSR 59
Query: 241 WDNMNT*NA*FLYVQHQRT*LCCHGN*EILNMVFTLY--XXSNVVSKADIKTLAECDDXX 414
+ +N + ++ + + E+ + Y SNV+SKA I+ +A D+
Sbjct: 60 -EALNHLSC-IAFLSPSGSSIEAVKT-ELAKPRYGNYWLFFSNVLSKAQIEEMASVDELE 116
Query: 415 AVRXVHXVFADY 450
V+ V FADY
Sbjct: 117 VVKEVQEYFADY 128
Score = 36.3 bits (80), Expect = 0.52
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = +3
Query: 255 HMKCIVFIRPTSENIALLSRELRDPKYGVYFIY 353
H+ CI F+ P+ +I + EL P+YG Y+++
Sbjct: 64 HLSCIAFLSPSGSSIEAVKTELAKPRYGNYWLF 96
>UniRef50_O49048 Cluster: Vacuolar protein sorting-associated
protein 45 homolog; n=4; Magnoliophyta|Rep: Vacuolar
protein sorting-associated protein 45 homolog -
Arabidopsis thaliana (Mouse-ear cress)
Length = 569
Score = 65.7 bits (153), Expect = 7e-10
Identities = 47/133 (35%), Positives = 73/133 (54%), Gaps = 3/133 (2%)
Frame = +1
Query: 61 MNVIQAVKMYITKMXXESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDS-HA 237
M ++ +V+ YI +M + GMKV+++D ET S VS+VYSQSE+LQKEV+L E IDS
Sbjct: 1 MVLVTSVRDYINRMLQDIS-GMKVLILDSETVSNVSIVYSQSELLQKEVFLVEMIDSISV 59
Query: 238 KWDNMNT*NA*FLYVQHQRT*LCCHGN*EILNMVFTLY--XXSNVVSKADIKTLAECDDX 411
++M+ A +Y + ++ N F Y SN++ I LA+ D+
Sbjct: 60 SKESMSHLKA--VYFIRPTSDNIQKLRYQLANPRFGEYHLFFSNLLKDTQIHILADSDEQ 117
Query: 412 XAVRXVHXVFADY 450
V+ V +AD+
Sbjct: 118 EVVQQVQEYYADF 130
Score = 40.7 bits (91), Expect = 0.024
Identities = 15/33 (45%), Positives = 24/33 (72%)
Frame = +3
Query: 255 HMKCIVFIRPTSENIALLSRELRDPKYGVYFIY 353
H+K + FIRPTS+NI L +L +P++G Y ++
Sbjct: 66 HLKAVYFIRPTSDNIQKLRYQLANPRFGEYHLF 98
>UniRef50_Q0TYN0 Cluster: Putative uncharacterized protein; n=1;
Phaeosphaeria nodorum|Rep: Putative uncharacterized
protein - Phaeosphaeria nodorum (Septoria nodorum)
Length = 506
Score = 46.8 bits (106), Expect(2) = 8e-10
Identities = 27/63 (42%), Positives = 36/63 (57%), Gaps = 8/63 (12%)
Frame = +1
Query: 61 MNVIQAVKMYITKMXXESGPG--------MKVILMDKETTSIVSMVYSQSEILQKEVYLF 216
M+++QAV YITKM MK++L+D ET SIVS +QS +L EVYL
Sbjct: 1 MDIVQAVSGYITKMVSAGDSAATGTTAAKMKILLLDNETVSIVSTATTQSALLSHEVYLT 60
Query: 217 ERI 225
+ I
Sbjct: 61 DNI 63
Score = 38.7 bits (86), Expect(2) = 8e-10
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = +1
Query: 346 LYXXSNVVSKADIKTLAECDDXXAVRXVHXVFADYPGXGXETCSLS 483
+Y N++ K+ ++ LAE DD VR +H FAD+ + SL+
Sbjct: 57 VYLTDNIIKKSSLERLAEADDHEVVRAIHEYFADFLVINPDLMSLN 102
>UniRef50_A0BVV2 Cluster: Chromosome undetermined scaffold_130,
whole genome shotgun sequence; n=2; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_130,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 603
Score = 62.5 bits (145), Expect = 7e-09
Identities = 43/135 (31%), Positives = 73/135 (54%), Gaps = 5/135 (3%)
Frame = +1
Query: 61 MNVIQAVKMYITKMXXESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSHAK 240
+N++ + YI ++ E GMK +++D+ET I+S++YSQS+IL+K+VYL E+I++ A
Sbjct: 15 LNLLNSATDYIDRILSEVS-GMKCLILDQETIGIISLIYSQSQILKKDVYLMEKIEADAS 73
Query: 241 W-DNMNT*NA*FLY--VQHQRT*LCCHGN*EILNMVFTLY--XXSNVVSKADIKTLAECD 405
+ FL Q +T L EI + F Y +N +S I+ LAE D
Sbjct: 74 TKQKLQHMKVIFLIRPTQENQTLLLQ----EIKDKRFCEYYIFFTNTLSNFYIEQLAEAD 129
Query: 406 DXXAVRXVHXVFADY 450
++ + ++ DY
Sbjct: 130 GSDLIKQLQEIYLDY 144
>UniRef50_A3LRH9 Cluster: Vacuolar protein sorting associated
protein; n=4; Saccharomycetaceae|Rep: Vacuolar protein
sorting associated protein - Pichia stipitis (Yeast)
Length = 614
Score = 62.5 bits (145), Expect = 7e-09
Identities = 42/139 (30%), Positives = 74/139 (53%), Gaps = 5/139 (3%)
Frame = +1
Query: 49 NLIKMNVIQAVKMYITKMXXESGPG---MKVILMDKETTSIVSMVYSQSEILQKEVYLFE 219
NL K++ + K++ + S KV+L+DK TT I+SM Y+QS++LQ ++ L E
Sbjct: 4 NLAKVSNVYFEKLFAVQRNESSTNSEIKAKVLLVDKFTTPIISMCYTQSQLLQNDIILIE 63
Query: 220 RIDSHAKWDNMNT*NA*FLYVQHQRT*LCCHGN*EILNMVFTLY--XXSNVVSKADIKTL 393
I++ + + M N +Y++ R + E+ N F+ Y +N +SK +++ L
Sbjct: 64 LIENQSTLNVMKHLNC-IVYIKPTRESIQSLIK-ELNNPHFSKYQLFLNNTISKGELERL 121
Query: 394 AECDDXXAVRXVHXVFADY 450
AE D+ + V +F DY
Sbjct: 122 AEADEFEVINQVTEIFQDY 140
>UniRef50_P38932 Cluster: Vacuolar protein sorting-associated
protein 45; n=4; Saccharomycetaceae|Rep: Vacuolar
protein sorting-associated protein 45 - Saccharomyces
cerevisiae (Baker's yeast)
Length = 577
Score = 60.9 bits (141), Expect = 2e-08
Identities = 40/111 (36%), Positives = 64/111 (57%), Gaps = 2/111 (1%)
Frame = +1
Query: 124 MKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSHAKWDNMNT*NA*FLYVQHQRT*L 303
+KV+L+DK TT +S+ +QSE+L+ E+YL ERI++ + ++ +YV+ L
Sbjct: 32 IKVLLLDKNTTPTISLCATQSELLKHEIYLVERIENEQR--EVSRHLRCLVYVKPTEETL 89
Query: 304 CCHGN*EILNMVFTLY--XXSNVVSKADIKTLAECDDXXAVRXVHXVFADY 450
H E+ N + Y SN+VSK+ ++ LAE DD AV V +F D+
Sbjct: 90 -QHLLRELRNPRYGEYQIFFSNIVSKSQLERLAESDDLEAVTKVEEIFQDF 139
Score = 45.6 bits (103), Expect = 8e-04
Identities = 16/33 (48%), Positives = 26/33 (78%)
Frame = +3
Query: 255 HMKCIVFIRPTSENIALLSRELRDPKYGVYFIY 353
H++C+V+++PT E + L RELR+P+YG Y I+
Sbjct: 75 HLRCLVYVKPTEETLQHLLRELRNPRYGEYQIF 107
>UniRef50_A2X4A8 Cluster: Putative uncharacterized protein; n=2;
Oryza sativa|Rep: Putative uncharacterized protein -
Oryza sativa subsp. indica (Rice)
Length = 543
Score = 60.1 bits (139), Expect = 4e-08
Identities = 41/132 (31%), Positives = 70/132 (53%), Gaps = 2/132 (1%)
Frame = +1
Query: 61 MNVIQAVKMYITKMXXESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSHAK 240
M +I ++ YI +M + PGMKV+++D +T +VS+VYSQS++L+KEV+L E +D +A
Sbjct: 1 MTLITLIRDYIDRMLHDI-PGMKVLVLDPDTVGMVSVVYSQSDLLRKEVFLVETVD-NAS 58
Query: 241 WDNMNT*NA*FLYVQHQRT*LCCHGN*EILNMVFTLY--XXSNVVSKADIKTLAECDDXX 414
+ + +Y + + F Y SNV+ I+ LA+ D+
Sbjct: 59 SSRESMAHLKAVYFLRPSSDNVQKLRRHLAAPRFAEYHLFFSNVLKIPQIQVLADSDEQE 118
Query: 415 AVRXVHXVFADY 450
V+ V +AD+
Sbjct: 119 VVQQVQEFYADF 130
Score = 36.3 bits (80), Expect = 0.52
Identities = 12/33 (36%), Positives = 22/33 (66%)
Frame = +3
Query: 255 HMKCIVFIRPTSENIALLSRELRDPKYGVYFIY 353
H+K + F+RP+S+N+ L R L P++ Y ++
Sbjct: 66 HLKAVYFLRPSSDNVQKLRRHLAAPRFAEYHLF 98
>UniRef50_Q236N2 Cluster: Sec1 family protein; n=1; Tetrahymena
thermophila SB210|Rep: Sec1 family protein - Tetrahymena
thermophila SB210
Length = 593
Score = 60.1 bits (139), Expect = 4e-08
Identities = 40/132 (30%), Positives = 70/132 (53%), Gaps = 2/132 (1%)
Frame = +1
Query: 61 MNVIQAVKMYITKMXXESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSHAK 240
++ + ++ Y +M + G+K +++D ETT I+S+++SQS+IL+K+VYL ERI+ +
Sbjct: 11 LSPFKVLEDYFERMLQQVS-GIKCLVLDVETTPIISLIFSQSKILKKDVYLIERIEMPSD 69
Query: 241 WDNMNT*NA*FLYVQHQRT*LCCHGN*EILNMVFTLY--XXSNVVSKADIKTLAECDDXX 414
+ F+ + L EI F Y SN V I++LA+ D
Sbjct: 70 GKMQHLKAIYFIRPTEENLKLL---QKEIEKPRFAEYYIFYSNSVPNLTIESLAQIDTND 126
Query: 415 AVRXVHXVFADY 450
++ +H V+ADY
Sbjct: 127 YIKEIHEVYADY 138
Score = 45.2 bits (102), Expect = 0.001
Identities = 16/34 (47%), Positives = 25/34 (73%)
Frame = +3
Query: 252 EHMKCIVFIRPTSENIALLSRELRDPKYGVYFIY 353
+H+K I FIRPT EN+ LL +E+ P++ Y+I+
Sbjct: 73 QHLKAIYFIRPTEENLKLLQKEIEKPRFAEYYIF 106
>UniRef50_Q1EPY2 Cluster: EhVps45A; n=2; Entamoeba histolytica|Rep:
EhVps45A - Entamoeba histolytica
Length = 529
Score = 56.8 bits (131), Expect = 3e-07
Identities = 39/132 (29%), Positives = 67/132 (50%), Gaps = 2/132 (1%)
Frame = +1
Query: 61 MNVIQAVKMYITKMXXESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSHAK 240
MNVI A++ Y+ E+ PGMK ++MD +T +VS+++ +EI+QKEVYL +++ +
Sbjct: 1 MNVIHALQEYLNFTFSET-PGMKALIMDSDTIPVVSILFGMTEIIQKEVYLVQQLSDQTR 59
Query: 241 WDNMNT*NA*FLYVQHQRT*LCCHGN*EILNMVFTLY--XXSNVVSKADIKTLAECDDXX 414
D + NA L + E+ N + Y +N + I L++ D
Sbjct: 60 -DTLPHLNAICLLRPTKENMELLRK--ELNNPKYGKYYLFFTNFLDSTQISLLSQSDVHE 116
Query: 415 AVRXVHXVFADY 450
V+ V ++ DY
Sbjct: 117 VVQKVMELYVDY 128
>UniRef50_Q6FS71 Cluster: Candida glabrata strain CBS138 chromosome
H complete sequence; n=1; Candida glabrata|Rep: Candida
glabrata strain CBS138 chromosome H complete sequence -
Candida glabrata (Yeast) (Torulopsis glabrata)
Length = 589
Score = 55.6 bits (128), Expect = 8e-07
Identities = 21/33 (63%), Positives = 29/33 (87%)
Frame = +3
Query: 255 HMKCIVFIRPTSENIALLSRELRDPKYGVYFIY 353
H++CIV+++PT+++I LSRELRDPKYG Y IY
Sbjct: 75 HLRCIVYVKPTNDSIEFLSRELRDPKYGDYHIY 107
Score = 50.8 bits (116), Expect = 2e-05
Identities = 35/111 (31%), Positives = 58/111 (52%), Gaps = 2/111 (1%)
Frame = +1
Query: 124 MKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSHAKWDNMNT*NA*FLYVQHQRT*L 303
+K +L+DK T S +SM +QSE+L E++L + +++ + + +YV+ +
Sbjct: 32 IKALLLDKNTKSTISMCATQSELLHNEIFLIDTLENENRDPERHL--RCIVYVKPTNDSI 89
Query: 304 CCHGN*EILNMVFTLY--XXSNVVSKADIKTLAECDDXXAVRXVHXVFADY 450
E+ + + Y SN VSKA ++ LAE DD AV V +F DY
Sbjct: 90 EFLSR-ELRDPKYGDYHIYFSNSVSKAQLEKLAESDDMEAVSKVEEIFQDY 139
>UniRef50_A7TEA7 Cluster: Putative uncharacterized protein; n=1;
Vanderwaltozyma polyspora DSM 70294|Rep: Putative
uncharacterized protein - Vanderwaltozyma polyspora DSM
70294
Length = 594
Score = 55.6 bits (128), Expect = 8e-07
Identities = 40/121 (33%), Positives = 63/121 (52%), Gaps = 2/121 (1%)
Frame = +1
Query: 124 MKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSHAKWDNMNT*NA*FLYVQHQRT*L 303
+K +L+DK TTS +SM +QS++L +YL + I++ + D M +YV+
Sbjct: 31 VKALLLDKNTTSTISMCATQSDLLNHNIYLIDTIENKNR-DTMRHVKC-IVYVKPSDD-T 87
Query: 304 CCHGN*EILNMVFTLY--XXSNVVSKADIKTLAECDDXXAVRXVHXVFADYPGXGXETCS 477
+ E+ N + Y SN V+K+ ++ LAE DD AV V +F DY G + S
Sbjct: 88 IEYLLEELQNPRYADYYIYFSNTVNKSQLERLAESDDMEAVSKVEEIFQDYQILGEDLFS 147
Query: 478 L 480
L
Sbjct: 148 L 148
Score = 43.6 bits (98), Expect = 0.003
Identities = 15/33 (45%), Positives = 25/33 (75%)
Frame = +3
Query: 255 HMKCIVFIRPTSENIALLSRELRDPKYGVYFIY 353
H+KCIV+++P+ + I L EL++P+Y Y+IY
Sbjct: 74 HVKCIVYVKPSDDTIEYLLEELQNPRYADYYIY 106
>UniRef50_A2ESF3 Cluster: Sec1 family protein; n=2; Trichomonas
vaginalis G3|Rep: Sec1 family protein - Trichomonas
vaginalis G3
Length = 549
Score = 53.2 bits (122), Expect = 4e-06
Identities = 41/134 (30%), Positives = 71/134 (52%), Gaps = 4/134 (2%)
Frame = +1
Query: 61 MNVIQAVKMYITKMXXESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDS--H 234
MNV + +Y++K+ E+ PG+K +L+D+ET + +S+ +++E+L+KEV LFE + S H
Sbjct: 1 MNVQTSAYLYLSKIL-ETQPGVKALLLDQETFNFISVAMTKTELLEKEVVLFENLTSRVH 59
Query: 235 AKWDNMNT*NA*FLYVQHQRT*LCCHGN*EILNMVFTLY--XXSNVVSKADIKTLAECDD 408
D T ++V+ + E+ + F Y SN ++A I+ LA D
Sbjct: 60 KPEDPSCTSLNCIIFVRPTSDNVELISR-ELDHPHFQRYSIFFSNTSAEAHIRQLAAHDS 118
Query: 409 XXAVRXVHXVFADY 450
V V V+ D+
Sbjct: 119 QSLVDIVREVYLDF 132
Score = 42.3 bits (95), Expect = 0.008
Identities = 16/32 (50%), Positives = 24/32 (75%)
Frame = +3
Query: 258 MKCIVFIRPTSENIALLSRELRDPKYGVYFIY 353
+ CI+F+RPTS+N+ L+SREL P + Y I+
Sbjct: 69 LNCIIFVRPTSDNVELISRELDHPHFQRYSIF 100
>UniRef50_Q09805 Cluster: Putative vacuolar protein
sorting-associated protein C2G11.03c; n=1;
Schizosaccharomyces pombe|Rep: Putative vacuolar protein
sorting-associated protein C2G11.03c -
Schizosaccharomyces pombe (Fission yeast)
Length = 558
Score = 51.2 bits (117), Expect = 2e-05
Identities = 36/131 (27%), Positives = 68/131 (51%), Gaps = 1/131 (0%)
Frame = +1
Query: 61 MNVIQAVKMYITKMXXESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERI-DSHA 237
M+++ A + Y ++ E +K++L++++TT IVS +QS +L++++YL + +
Sbjct: 1 MDLVSASQSYFKRIFQEVSD-LKILLLEEDTTKIVSSCITQSNLLEQQIYLTVLLGNKRE 59
Query: 238 KWDNMNT*NA*FLYVQHQRT*LCCHGN*EILNMVFTLYXXSNVVSKADIKTLAECDDXXA 417
K ++ FL L C + + LY +NV+ K+ ++ LAE DD A
Sbjct: 60 KLRHLKC--VAFLRPTPTTLRLLCEELRDPKYAEYHLY-FTNVIPKSFLERLAESDDFEA 116
Query: 418 VRXVHXVFADY 450
V+ + F DY
Sbjct: 117 VKSIQEFFLDY 127
Score = 48.4 bits (110), Expect = 1e-04
Identities = 18/33 (54%), Positives = 23/33 (69%)
Frame = +3
Query: 255 HMKCIVFIRPTSENIALLSRELRDPKYGVYFIY 353
H+KC+ F+RPT + LL ELRDPKY Y +Y
Sbjct: 63 HLKCVAFLRPTPTTLRLLCEELRDPKYAEYHLY 95
>UniRef50_A4RVJ6 Cluster: Predicted protein; n=2; Ostreococcus|Rep:
Predicted protein - Ostreococcus lucimarinus CCE9901
Length = 565
Score = 49.6 bits (113), Expect = 5e-05
Identities = 40/144 (27%), Positives = 71/144 (49%), Gaps = 4/144 (2%)
Frame = +1
Query: 61 MNVIQAVKMYITKMXXESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDS--H 234
M++ +A K Y+ +M + GMK ++ D T +I+S+V SQSE+L +EV+L E++ H
Sbjct: 1 MDLSEAHKEYVKRMLDVAKGGMKALVCDPVTVNILSVVMSQSEVLAREVFLIEQLHERPH 60
Query: 235 AKWDNMNT*NA*FLYVQHQRT*LCCHGN*EILNMVFTLY--XXSNVVSKADIKTLAECDD 408
+ ++ ++V+ R + G ++ + Y SNV + ++ LA DD
Sbjct: 61 EEMPHLKA----VVFVRPTRENVRTLGK-QLKQRTYGEYHVFFSNVCPEGLLQELAAEDD 115
Query: 409 XXAVRXVHXVFADYPGXGXETCSL 480
V V +AD T +L
Sbjct: 116 DELVVQVQEYYADATAVDRNTFAL 139
>UniRef50_A1CM48 Cluster: Vacuolar protein sorting-associated
protein VpsB; n=15; Ascomycota|Rep: Vacuolar protein
sorting-associated protein VpsB - Aspergillus clavatus
Length = 608
Score = 47.2 bits (107), Expect = 3e-04
Identities = 49/166 (29%), Positives = 77/166 (46%), Gaps = 25/166 (15%)
Frame = +1
Query: 61 MNVIQAVKMYITKMXX----------ESGPGMKVILMDKETTS-------------IVSM 171
M+V+ AV YI+KM S MK++L+D ET IVS
Sbjct: 1 MDVVAAVSGYISKMVTTGDSSSVSGSSSSTKMKILLLDSETVGSAFYCWVRLKLMPIVST 60
Query: 172 VYSQSEILQKEVYLFERIDSHAKWDNMNT*NA*FLYVQHQRT*LCCHGN*EILNMVFTLY 351
+QS +L EVYL +R+D+ A+ + M +V+ T + + E+ + + Y
Sbjct: 61 AITQSALLNHEVYLIDRLDNAAR-EKMRHLRC-LCFVRPSPTSIQFLID-ELRDPKYGEY 117
Query: 352 XX--SNVVSKADIKTLAECDDXXAVRXVHXVFADYPGXGXETCSLS 483
SN++ K+ ++ LAE D VR V FAD+ + CSL+
Sbjct: 118 HIYLSNIIRKSSLERLAEADGHEVVRVVQEHFADFLVINPDLCSLN 163
Score = 46.8 bits (106), Expect = 4e-04
Identities = 18/33 (54%), Positives = 24/33 (72%)
Frame = +3
Query: 255 HMKCIVFIRPTSENIALLSRELRDPKYGVYFIY 353
H++C+ F+RP+ +I L ELRDPKYG Y IY
Sbjct: 88 HLRCLCFVRPSPTSIQFLIDELRDPKYGEYHIY 120
>UniRef50_A2F2C8 Cluster: Sec1 family protein; n=1; Trichomonas
vaginalis G3|Rep: Sec1 family protein - Trichomonas
vaginalis G3
Length = 535
Score = 46.4 bits (105), Expect = 5e-04
Identities = 19/55 (34%), Positives = 37/55 (67%)
Frame = +1
Query: 61 MNVIQAVKMYITKMXXESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERI 225
MN +++++ YI + G K++++D ETT I+ ++YS++E+LQ +V L + I
Sbjct: 1 MNAVESLRAYIDSILPNV-EGPKILVLDDETTKIIGLIYSKTELLQHDVVLIDTI 54
Score = 36.3 bits (80), Expect = 0.52
Identities = 14/32 (43%), Positives = 21/32 (65%)
Frame = +3
Query: 258 MKCIVFIRPTSENIALLSRELRDPKYGVYFIY 353
++C+ +RPT E I LS EL P YG Y+++
Sbjct: 69 IQCVCILRPTHEVIRDLSNELNTPHYGSYYLF 100
>UniRef50_Q5ABT2 Cluster: Putative uncharacterized protein VPS45;
n=1; Candida albicans|Rep: Putative uncharacterized
protein VPS45 - Candida albicans (Yeast)
Length = 652
Score = 45.6 bits (103), Expect = 8e-04
Identities = 30/110 (27%), Positives = 57/110 (51%), Gaps = 2/110 (1%)
Frame = +1
Query: 127 KVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSHAKWDNMNT*NA*FLYVQHQRT*LC 306
+V+L+DK TT I+SM Y+QSE+L + L E + + +M + +Y++ + +
Sbjct: 46 RVLLLDKYTTPIISMCYTQSELLANNIILIELLQYYHDLSSMKHLDC-IIYIKPCQESVN 104
Query: 307 CHGN*EILNMVFTLY--XXSNVVSKADIKTLAECDDXXAVRXVHXVFADY 450
E+ N + Y +N + K ++++AE D+ + V +F DY
Sbjct: 105 DLRQ-ELHNPHYGQYKLFLNNCIHKNQLESIAEADEYEVITKVIEIFQDY 153
Score = 39.5 bits (88), Expect = 0.055
Identities = 14/38 (36%), Positives = 25/38 (65%)
Frame = +3
Query: 252 EHMKCIVFIRPTSENIALLSRELRDPKYGVYFIYXQ*C 365
+H+ CI++I+P E++ L +EL +P YG Y ++ C
Sbjct: 88 KHLDCIIYIKPCQESVNDLRQELHNPHYGQYKLFLNNC 125
>UniRef50_Q1EPY1 Cluster: EhVps45B; n=1; Entamoeba histolytica|Rep:
EhVps45B - Entamoeba histolytica
Length = 542
Score = 40.7 bits (91), Expect = 0.024
Identities = 16/35 (45%), Positives = 25/35 (71%)
Frame = +1
Query: 121 GMKVILMDKETTSIVSMVYSQSEILQKEVYLFERI 225
G+K +++D TTSIVS +YS ++ QKE+Y+ I
Sbjct: 22 GLKALVLDSVTTSIVSHLYSMMDVTQKEIYIITNI 56
>UniRef50_A2GDG9 Cluster: Sec1 family protein; n=3; Trichomonas
vaginalis G3|Rep: Sec1 family protein - Trichomonas
vaginalis G3
Length = 543
Score = 39.5 bits (88), Expect = 0.055
Identities = 40/136 (29%), Positives = 62/136 (45%), Gaps = 5/136 (3%)
Frame = +1
Query: 61 MNVIQAVKMYITKMXXESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFER----ID 228
MNV A YI+ + G KV+L D+ET I+SM +++E+L EV L E I+
Sbjct: 1 MNVQVASYKYISFILAGLA-GPKVVLFDEETLPIISMSMTKTELLSLEVVLTESFTKIIE 59
Query: 229 SHAKWDNMNT*NA*FLYVQHQRT*LCCHGN*EILNMVFTLYXXSNVVSKADIKTLAECDD 408
D + FL C+ M +TL +NVVS+A ++ +A D+
Sbjct: 60 GPYSADLQSLPAICFLRPTDDNVNYICNELSHPHFMKYTL-VFTNVVSEAFLRQIASRDN 118
Query: 409 XXAVRXVHXVFAD-YP 453
+ + + D YP
Sbjct: 119 NSLINSIQEAYLDVYP 134
>UniRef50_Q4Q1J8 Cluster: Vacuolar protein sorting-associated
protein 45-like protein; n=5; Trypanosomatidae|Rep:
Vacuolar protein sorting-associated protein 45-like
protein - Leishmania major
Length = 559
Score = 36.7 bits (81), Expect = 0.39
Identities = 16/43 (37%), Positives = 29/43 (67%)
Frame = +1
Query: 124 MKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSHAKWDNM 252
MKV+L+D +++ +SQ+E+L+ VYL E ++S A+ N+
Sbjct: 11 MKVLLVDDGALPMIATAFSQTELLKHGVYLVESLNSAARQRNL 53
>UniRef50_Q7P2P9 Cluster: Hypothetical Membrane Spanning Protein;
n=1; Fusobacterium nucleatum subsp. vincentii ATCC
49256|Rep: Hypothetical Membrane Spanning Protein -
Fusobacterium nucleatum subsp. vincentii ATCC 49256
Length = 383
Score = 35.9 bits (79), Expect = 0.68
Identities = 24/55 (43%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
Frame = +1
Query: 25 ILCEINKDNLIKMNVIQAVKMYITKM---XXESGPGMKVILMDKETTSIVSMVYS 180
IL + N+ NLIKMN I VK Y K+ ES P M++ DKE +V M S
Sbjct: 306 ILYKNNEINLIKMNSIDCVKFYSKKLGKGRKESNPTMQI--FDKEQKILVEMTIS 358
>UniRef50_Q4Q232 Cluster: Vacuolar protein sorting-associated
protein, putative; n=5; Trypanosomatidae|Rep: Vacuolar
protein sorting-associated protein, putative -
Leishmania major
Length = 617
Score = 35.9 bits (79), Expect = 0.68
Identities = 19/68 (27%), Positives = 36/68 (52%)
Frame = +1
Query: 40 NKDNLIKMNVIQAVKMYITKMXXESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFE 219
+K N + N ++ Y+ + G+KV+L D T I+S+ YSQ ++LQ V L +
Sbjct: 7 SKVNRGRRNCLEQAWNYLNTAFSATA-GLKVLLCDDATREILSVAYSQHQLLQHNVVLVD 65
Query: 220 RIDSHAKW 243
+ + ++
Sbjct: 66 MLANQERY 73
>UniRef50_A2EN46 Cluster: Sec1 family protein; n=1; Trichomonas
vaginalis G3|Rep: Sec1 family protein - Trichomonas
vaginalis G3
Length = 545
Score = 35.9 bits (79), Expect = 0.68
Identities = 19/59 (32%), Positives = 32/59 (54%)
Frame = +1
Query: 61 MNVIQAVKMYITKMXXESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSHA 237
MN++ A Y + E+ PG K ++MD T ++ + +++EIL EV + E I A
Sbjct: 1 MNILDASFEYFRDIFVEN-PGPKGLIMDDSTKKVLGLAITRTEILSYEVLITESISDLA 58
>UniRef50_O96243 Cluster: Vacuolar protein-sorting protein VPS45,
putative; n=7; Plasmodium|Rep: Vacuolar protein-sorting
protein VPS45, putative - Plasmodium falciparum (isolate
3D7)
Length = 722
Score = 34.7 bits (76), Expect = 1.6
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = +3
Query: 252 EHMKCIVFIRPTSENIALLSRELRDPKYGVYFIY 353
+H+K I +RPT NI L EL+ P + Y+I+
Sbjct: 93 KHLKVIFLLRPTYTNILRLMSELKKPLFSEYYIF 126
>UniRef50_Q8SRN0 Cluster: Putative VACUOLAR PROTEIN
SORTING-ASSOCIATED PROTEIN; n=1; Encephalitozoon
cuniculi|Rep: Putative VACUOLAR PROTEIN
SORTING-ASSOCIATED PROTEIN - Encephalitozoon cuniculi
Length = 490
Score = 33.9 bits (74), Expect = 2.8
Identities = 14/37 (37%), Positives = 24/37 (64%)
Frame = +1
Query: 115 GPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERI 225
G G+KV+L D++T I+S + S L+ + +LF+ I
Sbjct: 17 GEGVKVLLFDEDTKMILSNLIPHSRFLESDYFLFDSI 53
>UniRef50_UPI000069F4A8 Cluster: Hyaluronidase-1 precursor (EC
3.2.1.35) (Hyal-1) (Hyaluronoglucosaminidase-1)
(LUCA-1).; n=1; Xenopus tropicalis|Rep: Hyaluronidase-1
precursor (EC 3.2.1.35) (Hyal-1)
(Hyaluronoglucosaminidase-1) (LUCA-1). - Xenopus
tropicalis
Length = 356
Score = 32.3 bits (70), Expect = 8.4
Identities = 16/37 (43%), Positives = 18/37 (48%), Gaps = 2/37 (5%)
Frame = -1
Query: 319 SSRDNRAMFSDVGRIKTMHFMCSCYPIW--HDCLSSR 215
SS + + D RIK HF C CYP W DC R
Sbjct: 320 SSDSHLHLHPDSFRIKREHFYCRCYPGWDGEDCTRRR 356
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 514,936,367
Number of Sequences: 1657284
Number of extensions: 8851817
Number of successful extensions: 15278
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 14876
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 15244
length of database: 575,637,011
effective HSP length: 96
effective length of database: 416,537,747
effective search space used: 39571085965
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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