BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30335.Seq (681 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1682.14 |rpl1902|rpl19-2|60S ribosomal protein L19B|Schizosa... 120 3e-28 SPBC56F2.02 |rpl1901|rpl19-1|60S ribosomal protein L19|Schizosac... 120 3e-28 SPBC9B6.07 |||nucleolar protein Nop52 family |Schizosaccharomyce... 29 0.47 SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 29 0.62 SPBC28E12.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 29 0.82 SPCC4G3.18 |||conserved fungal family|Schizosaccharomyces pombe|... 27 1.9 SPAC1039.04 |||nicotinic acid plasma membrane transporter |Schiz... 26 4.4 SPBP23A10.12 |||FRG1 family protein|Schizosaccharomyces pombe|ch... 26 4.4 SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Sc... 26 5.8 >SPCC1682.14 |rpl1902|rpl19-2|60S ribosomal protein L19B|Schizosaccharomyces pombe|chr 3|||Manual Length = 193 Score = 120 bits (288), Expect = 3e-28 Identities = 52/77 (67%), Positives = 67/77 (87%) Frame = +1 Query: 22 MSSLKLQKRLAASVMRCGKKKVWLDPNEINEIANTNSRQNIRKMIKDGLVIKKPVAVHSR 201 M++L+ QKRLAASV++CGK+KVW+DPNEI+EI+N NSRQNIRK++KDGLVI+KP +HSR Sbjct: 1 MANLRTQKRLAASVLKCGKRKVWMDPNEISEISNANSRQNIRKLVKDGLVIRKPNLMHSR 60 Query: 202 ARVRKNTEARRKGRHCG 252 R+RK A+R GRH G Sbjct: 61 FRIRKTHAAKRLGRHTG 77 Score = 93.1 bits (221), Expect = 3e-20 Identities = 42/87 (48%), Positives = 57/87 (65%) Frame = +3 Query: 255 GKRRGTANARMPQKELWXXXXXXXXXXXXXXXTAKKIDRHLYHSLYMKAKGNVFKNKRVL 434 GKR+GTA ARMP +W + KID+HLYH+LY++AKGN FK+KR L Sbjct: 79 GKRKGTAEARMPSTVVWMRRQRVLRRLLRKYRESGKIDKHLYHTLYLEAKGNTFKHKRAL 138 Query: 435 MEYIHRKKAEKARTKMLSDQAEARRIK 515 +E+I R KAE RTK++ +Q +ARR + Sbjct: 139 IEHIQRAKAEANRTKLIQEQQDARRAR 165 >SPBC56F2.02 |rpl1901|rpl19-1|60S ribosomal protein L19|Schizosaccharomyces pombe|chr 2|||Manual Length = 193 Score = 120 bits (288), Expect = 3e-28 Identities = 52/77 (67%), Positives = 67/77 (87%) Frame = +1 Query: 22 MSSLKLQKRLAASVMRCGKKKVWLDPNEINEIANTNSRQNIRKMIKDGLVIKKPVAVHSR 201 M++L+ QKRLAASV++CGK+KVW+DPNEI+EI+N NSRQN+RK+IKDGLVI+KP +HSR Sbjct: 1 MANLRTQKRLAASVLKCGKRKVWMDPNEISEISNANSRQNVRKLIKDGLVIRKPNLMHSR 60 Query: 202 ARVRKNTEARRKGRHCG 252 R+RK A+R GRH G Sbjct: 61 FRIRKTHAAKRLGRHTG 77 Score = 93.1 bits (221), Expect = 3e-20 Identities = 42/87 (48%), Positives = 57/87 (65%) Frame = +3 Query: 255 GKRRGTANARMPQKELWXXXXXXXXXXXXXXXTAKKIDRHLYHSLYMKAKGNVFKNKRVL 434 GKR+GTA ARMP +W + KID+HLYH+LY++AKGN FK+KR L Sbjct: 79 GKRKGTAEARMPSAVVWMRRQRVLRRLLRKYRESGKIDKHLYHTLYLEAKGNTFKHKRAL 138 Query: 435 MEYIHRKKAEKARTKMLSDQAEARRIK 515 +E+I R KAE RTK++ +Q +ARR + Sbjct: 139 IEHIQRAKAEANRTKLIQEQQDARRAR 165 >SPBC9B6.07 |||nucleolar protein Nop52 family |Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 29.5 bits (63), Expect = 0.47 Identities = 12/30 (40%), Positives = 15/30 (50%) Frame = -2 Query: 224 SVFLRTRAREWTATGFLMTRPSLIILRMFC 135 S F T REWT L T +++R FC Sbjct: 85 STFWETMGREWTGIDILRTDKFYLLMRRFC 114 >SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharomyces pombe|chr 2|||Manual Length = 1309 Score = 29.1 bits (62), Expect = 0.62 Identities = 12/30 (40%), Positives = 21/30 (70%) Frame = -1 Query: 165 TILDHLTDVLSGVGVCDLIDFIWIQPHLLF 76 +I +H++DV G+ V LI+ + I+P +LF Sbjct: 351 SICEHVSDVSRGIAVETLIELMIIRPKILF 380 >SPBC28E12.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 356 Score = 28.7 bits (61), Expect = 0.82 Identities = 12/32 (37%), Positives = 19/32 (59%) Frame = -1 Query: 144 DVLSGVGVCDLIDFIWIQPHLLFTTSHNRGCK 49 +VLS + ++D + PHLL +S N+ CK Sbjct: 237 EVLSKLPYFHILDKSFFSPHLLLQSSENQACK 268 >SPCC4G3.18 |||conserved fungal family|Schizosaccharomyces pombe|chr 3|||Manual Length = 828 Score = 27.5 bits (58), Expect = 1.9 Identities = 17/42 (40%), Positives = 26/42 (61%) Frame = -2 Query: 515 LYSAGLSLVAKHLRPGLLSLLPVDVLHEHTLVLEHITLRLHV 390 +Y+ LSL K + + +LL + + H HTLV E +TL+L V Sbjct: 319 VYTVQLSLPVKSVESSVQALLFMVLPHLHTLVNE-LTLKLFV 359 >SPAC1039.04 |||nicotinic acid plasma membrane transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 507 Score = 26.2 bits (55), Expect = 4.4 Identities = 14/37 (37%), Positives = 18/37 (48%) Frame = -1 Query: 276 WQYLFSYQATVTTLSTCLCVFADTGAGVDCYRFLDDE 166 WQYLF + VT L + +F V+ FL DE Sbjct: 219 WQYLFLIEGGVTFL-CAIVIFLVLPVSVETANFLTDE 254 >SPBP23A10.12 |||FRG1 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 245 Score = 26.2 bits (55), Expect = 4.4 Identities = 11/38 (28%), Positives = 18/38 (47%) Frame = +1 Query: 4 GSHRVRMSSLKLQKRLAASVMRCGKKKVWLDPNEINEI 117 G +++ K K L +S+ W D NE+NE+ Sbjct: 9 GDKKIQKKKKKNTKSLQSSLESAENDPFWTDANELNEL 46 >SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 611 Score = 25.8 bits (54), Expect = 5.8 Identities = 11/32 (34%), Positives = 17/32 (53%), Gaps = 1/32 (3%) Frame = -3 Query: 460 AFFLWMYSMSTRLFLNTLPFAFM-*SEWYRCL 368 AF LW+YS+ L+L + W++CL Sbjct: 337 AFVLWVYSLDKELWLQLDMLGVLNHGSWFKCL 368 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,678,398 Number of Sequences: 5004 Number of extensions: 56123 Number of successful extensions: 162 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 154 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 160 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 313902888 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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