Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30328.Seq
         (878 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac...    99   4e-22
SPAC1556.02c |sdh1||succinate dehydrogenase Sdh1|Schizosaccharom...    30   0.38 
SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schi...    30   0.38 
SPAPJ698.02c |rps002|rpsa-2, rps0-2, rps0|40S ribosomal protein ...    30   0.38 
SPBC1A4.01 |apc10|SPBC1E8.06|anaphase-promoting complex subunit ...    29   0.87 
SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein S15a...    29   1.2  
SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|S...    29   1.2  
SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein S...    29   1.2  
SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schi...    28   1.5  
SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac...    28   2.0  
SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr...    27   3.5  
SPAC1952.16 |rga9||RhoGAp, GTPase activator towards Rho/Rac/Cdc4...    27   4.7  
SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizo...    26   6.1  
SPBP4G3.03 |||PI31 proteasome regulator related|Schizosaccharomy...    26   6.1  
SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   8.1  
SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr ...    26   8.1  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   8.1  

>SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase
           Cdc48|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 815

 Score =   99 bits (238), Expect = 4e-22
 Identities = 63/189 (33%), Positives = 90/189 (47%)
 Frame = +1

Query: 223 LAQGQTPQGNRCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 402
           + +G+  +    IVL+D+   D   R+             D+V+I PCP +KY +R+ +L
Sbjct: 78  VVKGKRRKDTVLIVLTDEEMEDGVARINRVVRNNLRVRLGDIVTINPCPDIKYAERISVL 137

Query: 403 PIDDSVEGLTGNLFEVYLKPYFMEAYRPIHRDDTFRSAGACAPSSSKWSKQIHHHFASWL 582
           P+ D+VEGLTG+LF+VYLKPYF+EAYRPI + D F   G+      K        F    
Sbjct: 138 PLADTVEGLTGSLFDVYLKPYFVEAYRPIRKGDLFVVRGSMRQVEFKVVDVAPDEFGIVS 197

Query: 583 LIP*YTVTVNRLNVRKKRKH*MLSAMMTSGGCRKQLGANLRRWWSCHCVILHWFKAIGSE 762
                      +N   +            GGCR+Q+ A +R            FK+IG +
Sbjct: 198 QDTIIHWEGEPINREDEESSLAEVGYDDIGGCRRQM-AQIRELVELPLRHPQLFKSIGIK 256

Query: 763 PPTRAFLMY 789
           PP R  LMY
Sbjct: 257 PP-RGILMY 264



 Score = 94.7 bits (225), Expect = 2e-20
 Identities = 57/125 (45%), Positives = 71/125 (56%), Gaps = 3/125 (2%)
 Frame = +3

Query: 510 VRGGMRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIKREEEEEALNAVGYDDIGRLSQTT 689
           VRG MR VEFKVV+  P  F IV+ DT+IH +GEPI RE+EE +L  VGYDDIG   +  
Sbjct: 174 VRGSMRQVEFKVVDVAPDEFGIVSQDTIIHWEGEPINREDEESSLAEVGYDDIGGCRRQM 233

Query: 690 GRKFKEMVELPLRHPSLVQGYWE*TSHAGISHVXGRLV---PGKNTPLLVAXCQXKQGAS 860
             + +E+VELPLRHP L +         GI    G L+   PG    L+      + GA 
Sbjct: 234 A-QIRELVELPLRHPQLFKS-------IGIKPPRGILMYGPPGTGKTLMARAVANETGAF 285

Query: 861 SF*IN 875
            F IN
Sbjct: 286 FFLIN 290



 Score = 88.6 bits (210), Expect = 1e-18
 Identities = 41/68 (60%), Positives = 53/68 (77%)
 Frame = +2

Query: 53  NKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLK 232
           N   D  S +D +TAILR+K +PN L+V++A +DDNSV+ LS   ME LQLFRGDTV++K
Sbjct: 21  NPPKDTYSAEDTATAILRKKRKPNSLVVDDATNDDNSVITLSSNTMETLQLFRGDTVVVK 80

Query: 233 GKRRKETV 256
           GKRRK+TV
Sbjct: 81  GKRRKDTV 88


>SPAC1556.02c |sdh1||succinate dehydrogenase
           Sdh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 641

 Score = 30.3 bits (65), Expect = 0.38
 Identities = 19/56 (33%), Positives = 28/56 (50%), Gaps = 1/56 (1%)
 Frame = -1

Query: 548 DHFELDGAHAPAD-LKVSSRWIGR*ASMKYGFKYTSNRLPVRPSTESSIGSMWTRF 384
           DH  L  +H PA+ LK     I   A++  G   T   +PV P+   ++G + TRF
Sbjct: 355 DHCYLQLSHLPAEILKERLPGISETAAIFAGVDVTKEPIPVLPTVHYNMGGIPTRF 410


>SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit
           Rpt2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 448

 Score = 30.3 bits (65), Expect = 0.38
 Identities = 17/48 (35%), Positives = 24/48 (50%)
 Frame = +3

Query: 603 DGEPIKREEEEEALNAVGYDDIGRLSQTTGRKFKEMVELPLRHPSLVQ 746
           D +P+    + +      Y DIG L     ++ KE VELPL HP L +
Sbjct: 172 DTDPMINVMKLDKAPTESYADIGGLESQI-QEIKEAVELPLTHPELYE 218


>SPAPJ698.02c |rps002|rpsa-2, rps0-2, rps0|40S ribosomal protein
           S0B|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 287

 Score = 30.3 bits (65), Expect = 0.38
 Identities = 16/44 (36%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
 Frame = +3

Query: 576 VAPDTVIHCDGEPIKREEEEEALNAVGYDDIGRL-SQTTGRKFK 704
           V PD   + D E I+REEE++A  A   ++  +L +QT   +F+
Sbjct: 200 VMPDLYFYRDPEEIEREEEQKAAAAAAAEEEAQLAAQTAAAEFE 243


>SPBC1A4.01 |apc10|SPBC1E8.06|anaphase-promoting complex subunit
           Apc10|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 189

 Score = 29.1 bits (62), Expect = 0.87
 Identities = 14/50 (28%), Positives = 24/50 (48%)
 Frame = +3

Query: 609 EPIKREEEEEALNAVGYDDIGRLSQTTGRKFKEMVELPLRHPSLVQGYWE 758
           E +K+++ E   +  G+ DIG L+Q T    K    + L     +  YW+
Sbjct: 7   EALKKQKSETQKSTEGFVDIGNLAQWTCSSEKSGFPIRLVRDDNIDTYWQ 56


>SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein
           S15a|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 130

 Score = 28.7 bits (61), Expect = 1.2
 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%)
 Frame = -2

Query: 421 QLNHQLAVCGLV---FHISLKDTERWI 350
           QLN ++  CG++   F++ LKD E+W+
Sbjct: 64  QLNGRINKCGVISPRFNVKLKDIEKWV 90


>SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit
           Rpt6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 403

 Score = 28.7 bits (61), Expect = 1.2
 Identities = 11/30 (36%), Positives = 21/30 (70%)
 Frame = +3

Query: 657 YDDIGRLSQTTGRKFKEMVELPLRHPSLVQ 746
           Y+ +G L +   ++ KE++ELP++HP L +
Sbjct: 144 YEMVGGLEKQI-KEIKEVIELPVKHPELFE 172


>SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein
           S15a|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 130

 Score = 28.7 bits (61), Expect = 1.2
 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%)
 Frame = -2

Query: 421 QLNHQLAVCGLV---FHISLKDTERWI 350
           QLN ++  CG++   F++ LKD E+W+
Sbjct: 64  QLNGRINKCGVISPRFNVKLKDIEKWV 90


>SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 319

 Score = 28.3 bits (60), Expect = 1.5
 Identities = 12/39 (30%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
 Frame = +3

Query: 78  LMIYRPRSSVARTDPTVSLSKKQSAMTTQSWH-FHRPKW 191
           L + R    +   DP ++ +  Q  + T+SWH FH  +W
Sbjct: 259 LALIRGCDGLLSDDPVMARALSQGPIVTKSWHYFHYSEW 297


>SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase
           E3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 905

 Score = 27.9 bits (59), Expect = 2.0
 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
 Frame = -2

Query: 391 LVFHISLKDTERWIP-HQKDAHEGCFSQHGSSEFSHQG 281
           LV  + LKD   WI    + + E  F   G ++FS+QG
Sbjct: 435 LVSDLYLKDLWSWIHLSHRQSEESLFGDTGDTDFSYQG 472


>SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 382

 Score = 27.1 bits (57), Expect = 3.5
 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 2/62 (3%)
 Frame = +3

Query: 27  FLDSNNKF*IKWQIIKALMIYRPRSSVARTDPTVS--LSKKQSAMTTQSWHFHRPKWSNF 200
           F+++N K   KW++  A+    P  S + + PT+S   + + SA TT     +RP  S  
Sbjct: 286 FIEANIK---KWRLAPAVGSLAPPPSYSTSQPTISWDAASESSAGTTTQGDTNRPDKSKN 342

Query: 201 NS 206
            S
Sbjct: 343 RS 344


>SPAC1952.16 |rga9||RhoGAp, GTPase activator towards
           Rho/Rac/Cdc42-like small GTPases|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 684

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 10/23 (43%), Positives = 13/23 (56%)
 Frame = -1

Query: 734 RMTQWQLHHLLKFAPSCLRQPPD 666
           RM   Q+ HL K    C+ +PPD
Sbjct: 587 RMKDGQIRHLAKIISPCVLRPPD 609


>SPCC1682.16 |rpt4||19S proteasome regulatory subunit
           Rpt4|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 388

 Score = 26.2 bits (55), Expect = 6.1
 Identities = 10/30 (33%), Positives = 21/30 (70%)
 Frame = +3

Query: 651 VGYDDIGRLSQTTGRKFKEMVELPLRHPSL 740
           + +  +G L++   R+ +E++ELPL++P L
Sbjct: 129 ISFAGVGGLNEQI-RELREVIELPLKNPEL 157


>SPBP4G3.03 |||PI31 proteasome regulator related|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 241

 Score = 26.2 bits (55), Expect = 6.1
 Identities = 14/48 (29%), Positives = 21/48 (43%)
 Frame = -2

Query: 391 LVFHISLKDTERWIPHQKDAHEGCFSQHGSSEFSHQGNYRLRARCNGF 248
           L FH ++ D   W PH+ +   G F  H     + +G +  R    GF
Sbjct: 177 LGFHPNVGDYFDWYPHRPNHFGGKFDGHSRGRGAGRGGFCGRHGRGGF 224


>SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 452

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 14/47 (29%), Positives = 25/47 (53%)
 Frame = +3

Query: 99  SSVARTDPTVSLSKKQSAMTTQSWHFHRPKWSNFNSSVVTQSCSRAN 239
           SSV       S S   ++M++ + +F+    S+ +SSV T + S +N
Sbjct: 329 SSVVIVQSETSCSINSASMSSNTTYFYWNSTSSLSSSVFTNTTSSSN 375


>SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 409

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 1/27 (3%)
 Frame = +2

Query: 77  PD-DLSTAILRRKDRPNRLIVEEAVSD 154
           PD D + + + RK+ PNRL++  A+S+
Sbjct: 233 PDADANISKIARKEDPNRLVLASAISE 259


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 14/53 (26%), Positives = 30/53 (56%)
 Frame = +2

Query: 50  LNKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLF 208
           L  +A N   + L+T   +RK + N  ++ +A ++ N+  +++ A  E+ QL+
Sbjct: 768 LGSLAGNLPQESLTTKS-KRKSKVNLELMFDATAEKNNENSMNSAVFEKSQLY 819


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,664,181
Number of Sequences: 5004
Number of extensions: 75769
Number of successful extensions: 228
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 216
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 227
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 440481800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)