Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30328.Seq
(878 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g03340.1 68418.m00286 cell division cycle protein 48, putativ... 119 3e-27
At3g09840.1 68416.m01174 cell division cycle protein 48 (CDC48A)... 119 3e-27
At3g53230.1 68416.m05865 cell division cycle protein 48, putativ... 113 1e-25
At5g20000.1 68418.m02380 26S proteasome AAA-ATPase subunit, puta... 33 0.33
At5g19990.1 68418.m02379 26S proteasome AAA-ATPase subunit (RPT6a) 33 0.33
At4g13885.1 68417.m02151 3'-5' exonuclease-related contains weak... 31 1.3
At3g26630.1 68416.m03328 pentatricopeptide (PPR) repeat-containi... 30 1.8
At4g29040.1 68417.m04153 26S proteasome AAA-ATPase subunit (RPT2... 30 2.3
At4g28000.1 68417.m04016 AAA-type ATPase family protein contains... 30 2.3
At2g20140.1 68415.m02353 26S protease regulatory complex subunit... 30 2.3
At4g02480.1 68417.m00335 AAA-type ATPase family protein contains... 29 5.4
At1g53750.1 68414.m06115 26S proteasome AAA-ATPase subunit (RPT1... 28 9.5
>At5g03340.1 68418.m00286 cell division cycle protein 48, putative /
CDC48, putative very strong similarity to SP|P54609 Cell
division cycle protein 48 homolog {Arabidopsis
thaliana}; contains Pfam profiles PF00004: ATPase AAA
family, PF02359: Cell division protein 48 (CDC48)
N-terminal domain; supporting cDNA
gi|26449351|dbj|AK117125.1|
Length = 810
Score = 119 bits (286), Expect = 3e-27
Identities = 73/191 (38%), Positives = 99/191 (51%), Gaps = 2/191 (1%)
Frame = +1
Query: 223 LAQGQTPQGNRCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 402
L +G+ + CI L+D+ C + KIRM DV+S+ CP VKYGKRVHIL
Sbjct: 62 LIKGKKRKDTVCIALADETCEEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGKRVHIL 121
Query: 403 PIDDSVEGLTGNLFEVYLKPYFMEAYRPIHRDDTFRSAGACAPSSSKWSKQIHHHFASWL 582
P+DD+VEG+TGNLF+ YLKPYF+EAYRP+ + D F G S K I A +
Sbjct: 122 PVDDTVEGVTGNLFDAYLKPYFLEAYRPVRKGDLFLVRGG---MRSVEFKVIETDPAEYC 178
Query: 583 LIP*YTVTVNRLNVRKKRKH*MLSAMMTS--GGCRKQLGANLRRWWSCHCVILHWFKAIG 756
++ T K+ L + GG RKQ+ A +R FK+IG
Sbjct: 179 VVAPDTEIFCEGEPVKREDEERLDEVGYDDVGGVRKQM-AQIRELVELPLRHPQLFKSIG 237
Query: 757 SEPPTRAFLMY 789
+PP + L+Y
Sbjct: 238 VKPP-KGILLY 247
Score = 109 bits (261), Expect = 3e-24
Identities = 59/125 (47%), Positives = 79/125 (63%), Gaps = 3/125 (2%)
Frame = +3
Query: 510 VRGGMRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIKREEEEEALNAVGYDDIGRLSQTT 689
VRGGMR+VEFKV+ETDP+ +C+VAPDT I C+GEP+KRE+EE L+ VGYDD+G + +
Sbjct: 158 VRGGMRSVEFKVIETDPAEYCVVAPDTEIFCEGEPVKREDEER-LDEVGYDDVGGVRKQM 216
Query: 690 GRKFKEMVELPLRHPSLVQGYWE*TSHAGISHVXGRLV---PGKNTPLLVAXCQXKQGAS 860
+ +E+VELPLRHP L + G+ G L+ PG L+ + GA
Sbjct: 217 A-QIRELVELPLRHPQLFKS-------IGVKPPKGILLYGPPGSGKTLIARAVANETGAF 268
Query: 861 SF*IN 875
F IN
Sbjct: 269 FFCIN 273
Score = 93.9 bits (223), Expect = 1e-19
Identities = 42/65 (64%), Positives = 55/65 (84%)
Frame = +2
Query: 62 ADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKR 241
+D+K+ D STAIL RK PNRL+V+EA++DDNSVV+L ME+LQLFRGDT+L+KGK+
Sbjct: 8 SDSKTKKDFSTAILERKKSPNRLVVDEAINDDNSVVSLHPTTMEKLQLFRGDTILIKGKK 67
Query: 242 RKETV 256
RK+TV
Sbjct: 68 RKDTV 72
>At3g09840.1 68416.m01174 cell division cycle protein 48 (CDC48A)
(CDC48) identical to SP|P54609 Cell division cycle
protein 48 homolog {Arabidopsis thaliana}
Length = 809
Score = 119 bits (286), Expect = 3e-27
Identities = 72/191 (37%), Positives = 102/191 (53%), Gaps = 2/191 (1%)
Frame = +1
Query: 223 LAQGQTPQGNRCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 402
L +G+ + CI L+D+ C + KIRM DV+S+ CP VKYGKRVHIL
Sbjct: 62 LIKGKKRKDTVCIALADETCEEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGKRVHIL 121
Query: 403 PIDDSVEGLTGNLFEVYLKPYFMEAYRPIHRDDTFRSAGACAPSSSKWSKQIHHHFASWL 582
P+DD+VEG+TGNLF+ YLKPYF+EAYRP+ + D F G S K I A +
Sbjct: 122 PVDDTVEGVTGNLFDAYLKPYFLEAYRPVRKGDLFLVRGG---MRSVEFKVIETDPAEYC 178
Query: 583 LI-P*YTVTVNRLNVRKKRKH*MLSAMMTS-GGCRKQLGANLRRWWSCHCVILHWFKAIG 756
++ P + V+++ + + GG RKQ+ A +R FK+IG
Sbjct: 179 VVAPDTEIFCEGEPVKREDEERLDDVGYDDVGGVRKQM-AQIRELVELPLRHPQLFKSIG 237
Query: 757 SEPPTRAFLMY 789
+PP + L+Y
Sbjct: 238 VKPP-KGILLY 247
Score = 108 bits (260), Expect = 4e-24
Identities = 59/125 (47%), Positives = 79/125 (63%), Gaps = 3/125 (2%)
Frame = +3
Query: 510 VRGGMRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIKREEEEEALNAVGYDDIGRLSQTT 689
VRGGMR+VEFKV+ETDP+ +C+VAPDT I C+GEP+KRE+EE L+ VGYDD+G + +
Sbjct: 158 VRGGMRSVEFKVIETDPAEYCVVAPDTEIFCEGEPVKREDEER-LDDVGYDDVGGVRKQM 216
Query: 690 GRKFKEMVELPLRHPSLVQGYWE*TSHAGISHVXGRLV---PGKNTPLLVAXCQXKQGAS 860
+ +E+VELPLRHP L + G+ G L+ PG L+ + GA
Sbjct: 217 A-QIRELVELPLRHPQLFKS-------IGVKPPKGILLYGPPGSGKTLIARAVANETGAF 268
Query: 861 SF*IN 875
F IN
Sbjct: 269 FFCIN 273
Score = 96.7 bits (230), Expect = 2e-20
Identities = 44/65 (67%), Positives = 56/65 (86%)
Frame = +2
Query: 62 ADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKR 241
+D+KS D STAIL RK PNRL+V+EA++DDNSVV+L A ME+LQLFRGDT+L+KGK+
Sbjct: 8 SDSKSKKDFSTAILERKKSPNRLVVDEAINDDNSVVSLHPATMEKLQLFRGDTILIKGKK 67
Query: 242 RKETV 256
RK+TV
Sbjct: 68 RKDTV 72
>At3g53230.1 68416.m05865 cell division cycle protein 48, putative /
CDC48, putative very strong similarity to SP|P54609 Cell
division cycle protein 48 homolog {Arabidopsis
thaliana}; contains Pfam profiles PF00004: ATPase AAA
family, PF02359: Cell division protein 48 (CDC48)
N-terminal domain
Length = 815
Score = 113 bits (272), Expect = 1e-25
Identities = 69/191 (36%), Positives = 98/191 (51%), Gaps = 2/191 (1%)
Frame = +1
Query: 223 LAQGQTPQGNRCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 402
L +G+ + CI L+D+ C + KIRM DV+S+ CP VKYG RVHIL
Sbjct: 63 LIKGKKRKDTVCIALADETCDEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGNRVHIL 122
Query: 403 PIDDSVEGLTGNLFEVYLKPYFMEAYRPIHRDDTFRSAGACAPSSSKWSKQIHHHFASWL 582
P+DD++EG++GN+F+ YLKPYF+EAYRP+ + D F G S K I A +
Sbjct: 123 PLDDTIEGVSGNIFDAYLKPYFLEAYRPVRKGDLFLVRGG---MRSIEFKVIETDPAEYC 179
Query: 583 LIP*YTVTVNRLNVRKKRKH*MLSAMMTS--GGCRKQLGANLRRWWSCHCVILHWFKAIG 756
++ T K+ L + GG RKQ+ A +R FK+IG
Sbjct: 180 VVAPDTEIFCEGEPIKREDEERLDEVGYDDVGGVRKQM-AQIRELVELPLRHPQLFKSIG 238
Query: 757 SEPPTRAFLMY 789
+PP + L+Y
Sbjct: 239 VKPP-KGILLY 248
Score = 109 bits (261), Expect = 3e-24
Identities = 59/125 (47%), Positives = 79/125 (63%), Gaps = 3/125 (2%)
Frame = +3
Query: 510 VRGGMRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIKREEEEEALNAVGYDDIGRLSQTT 689
VRGGMR++EFKV+ETDP+ +C+VAPDT I C+GEPIKRE+EE L+ VGYDD+G + +
Sbjct: 159 VRGGMRSIEFKVIETDPAEYCVVAPDTEIFCEGEPIKREDEER-LDEVGYDDVGGVRKQM 217
Query: 690 GRKFKEMVELPLRHPSLVQGYWE*TSHAGISHVXGRLV---PGKNTPLLVAXCQXKQGAS 860
+ +E+VELPLRHP L + G+ G L+ PG L+ + GA
Sbjct: 218 A-QIRELVELPLRHPQLFKS-------IGVKPPKGILLYGPPGSGKTLIARAVANETGAF 269
Query: 861 SF*IN 875
F IN
Sbjct: 270 FFCIN 274
Score = 84.2 bits (199), Expect = 1e-16
Identities = 40/66 (60%), Positives = 54/66 (81%), Gaps = 1/66 (1%)
Frame = +2
Query: 62 ADNK-SPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGK 238
+D+K + D STAIL +K NRL+V+EA++DDNSVV+L ME+LQLFRGDT+L+KGK
Sbjct: 8 SDSKGTKKDFSTAILEKKKAANRLVVDEAINDDNSVVSLHPDTMEKLQLFRGDTILIKGK 67
Query: 239 RRKETV 256
+RK+TV
Sbjct: 68 KRKDTV 73
>At5g20000.1 68418.m02380 26S proteasome AAA-ATPase subunit,
putative almost identical to 26S proteasome AAA-ATPase
subunit RPT6a GI:6652888 from [Arabidopsis thaliana];
almost identical to a member of conserved Sug1 CAD
family AtSUG1 GI:13537115 from [Arabidopsis thaliana]
Length = 419
Score = 32.7 bits (71), Expect = 0.33
Identities = 14/30 (46%), Positives = 21/30 (70%)
Frame = +3
Query: 657 YDDIGRLSQTTGRKFKEMVELPLRHPSLVQ 746
YD IG L Q ++ KE++ELP++HP L +
Sbjct: 160 YDMIGGLDQQI-KEIKEVIELPIKHPELFE 188
>At5g19990.1 68418.m02379 26S proteasome AAA-ATPase subunit (RPT6a)
Length = 419
Score = 32.7 bits (71), Expect = 0.33
Identities = 14/30 (46%), Positives = 21/30 (70%)
Frame = +3
Query: 657 YDDIGRLSQTTGRKFKEMVELPLRHPSLVQ 746
YD IG L Q ++ KE++ELP++HP L +
Sbjct: 160 YDMIGGLDQQI-KEIKEVIELPIKHPELFE 188
>At4g13885.1 68417.m02151 3'-5' exonuclease-related contains weak
similarity to Pfam domain PF01612: 3'-5' exonuclease
Length = 263
Score = 30.7 bits (66), Expect = 1.3
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = -2
Query: 214 TTEELKLLHFGL*KCHD*VVIAD 146
TTEELK+ H+ L KC D +V+A+
Sbjct: 3 TTEELKISHYKLYKCFDFLVVAN 25
>At3g26630.1 68416.m03328 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile: PF01535 PPR repeat
Length = 455
Score = 30.3 bits (65), Expect = 1.8
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = -3
Query: 369 RTRSDGYHIRKTHTKVVSHNTVHPNFLIRAII 274
RT S+ +++ HTK++ HN + L+R +I
Sbjct: 28 RTCSNFSQLKQIHTKIIKHNLTNDQLLVRQLI 59
>At4g29040.1 68417.m04153 26S proteasome AAA-ATPase subunit (RPT2a)
almost identical to 26S proteasome AAA-ATPase subunit
RPT2a (GI:6652880) {Arabidopsis thaliana}; Drosophila
melanogaster 26S proteasome subunit 4 ATPase,
PID:g1066065
Length = 443
Score = 29.9 bits (64), Expect = 2.3
Identities = 17/52 (32%), Positives = 26/52 (50%)
Frame = +3
Query: 591 VIHCDGEPIKREEEEEALNAVGYDDIGRLSQTTGRKFKEMVELPLRHPSLVQ 746
++ + +P+ + E Y DIG L ++ KE VELPL HP L +
Sbjct: 165 ILQDEVDPMVSVMKVEKAPLESYADIGGLEAQI-QEIKEAVELPLTHPELYE 215
>At4g28000.1 68417.m04016 AAA-type ATPase family protein contains
Pfam domain, PF00004: ATPase, AAA family
Length = 726
Score = 29.9 bits (64), Expect = 2.3
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = +3
Query: 651 VGYDDIGRLSQTTGRKFKEMVELPLRHPSLVQG 749
V + DIG L +T +E+V LPLR P L +G
Sbjct: 411 VTFADIGSLDETK-ESLQELVMLPLRRPDLFKG 442
>At2g20140.1 68415.m02353 26S protease regulatory complex subunit 4,
putative similar to Swiss-Prot:P48601 26S protease
regulatory subunit 4 (P26S4) [Drosophila melanogaster]
Length = 443
Score = 29.9 bits (64), Expect = 2.3
Identities = 17/52 (32%), Positives = 26/52 (50%)
Frame = +3
Query: 591 VIHCDGEPIKREEEEEALNAVGYDDIGRLSQTTGRKFKEMVELPLRHPSLVQ 746
++ + +P+ + E Y DIG L ++ KE VELPL HP L +
Sbjct: 165 ILQDEVDPMVSVMKVEKAPLESYADIGGLEAQI-QEIKEAVELPLTHPELYE 215
>At4g02480.1 68417.m00335 AAA-type ATPase family protein contains Pfam
domain, PF00004: ATPase, AAA family; similar to Spastin
(Swiss-Prot:Q9UBP0) [Homo sapiens] and Spastin (Fragment)
(Swiss-Prot:Q9QYY8) [Mus musculus]; similar to
mitochondrial sorting protein 1 (MSP1) protein
(TAT-binding homolog 4) (Swiss-Prot:P28737)
[Saccharomyces cerevisiae]
Length = 1265
Score = 28.7 bits (61), Expect = 5.4
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = +3
Query: 651 VGYDDIGRLSQTTGRKFKEMVELPLRHPSL 740
V +DDIG L + KE+V LPL+ P L
Sbjct: 960 VSFDDIGAL-ENVKETLKELVMLPLQRPEL 988
>At1g53750.1 68414.m06115 26S proteasome AAA-ATPase subunit (RPT1a)
similar to 26S proteasome ATPase subunit GI:1395190 from
[Spinacia oleracea]
Length = 426
Score = 27.9 bits (59), Expect = 9.5
Identities = 12/28 (42%), Positives = 18/28 (64%)
Frame = +3
Query: 651 VGYDDIGRLSQTTGRKFKEMVELPLRHP 734
V Y+D+G + K +E+VELP+ HP
Sbjct: 165 VTYNDVGGCKEQI-EKMREVVELPMLHP 191
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,853,773
Number of Sequences: 28952
Number of extensions: 423877
Number of successful extensions: 1198
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1192
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2067932800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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