BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30321.Seq (904 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac... 192 4e-50 SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schi... 28 1.6 SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr... 27 3.6 SPAC1556.02c |sdh1||succinate dehydrogenase Sdh1|Schizosaccharom... 27 4.8 SPAC144.07c |||conserved eukaryotic protein|Schizosaccharomyces ... 26 6.4 SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 26 6.4 SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit ... 26 6.4 SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.4 SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr ... 26 8.4 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.4 >SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosaccharomyces pombe|chr 1|||Manual Length = 815 Score = 192 bits (469), Expect = 4e-50 Identities = 116/275 (42%), Positives = 155/275 (56%), Gaps = 2/275 (0%) Frame = +2 Query: 53 NKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLK 232 N D S +D +TAILR+K +PN L+V++A +DDNSV+ LS ME LQLFRGDTV++K Sbjct: 21 NPPKDTYSAEDTATAILRKKRKPNSLVVDDATNDDNSVITLSSNTMETLQLFRGDTVVVK 80 Query: 233 GKRRKETV--ASCSQTIIALMRKFG*TVLXXTNFGXXXPXGEPXVXGPSVKYGKRVHILP 406 GKRRK+TV + + + + V N P +KY +R+ +LP Sbjct: 81 GKRRKDTVLIVLTDEEMEDGVARINRVV--RNNLRVRLGDIVTINPCPDIKYAERISVLP 138 Query: 407 IXDSXEGLTGNLFEVYLKPYFMXAYRPIHRDDTFWSAGACAPSSSKWSKQIHHHFASWAP 586 + D+ EGLTG+LF+VYLKPYF+ AYRPI + D F G+ K F + Sbjct: 139 LADTVEGLTGSLFDVYLKPYFVEAYRPIRKGDLFVVRGSMRQVEFKVVDVAPDEFGIVSQ 198 Query: 587 DTVIHCDGEPIKREEEEEALNAVGYDDIGGCRKQLAQIXGMGGXATXSXLHXSKAIXGEA 766 DT+IH +GEPI RE+EE +L VGYDDIGGCR+Q+AQI + K+I G Sbjct: 199 DTIIHWEGEPINREDEESSLAEVGYDDIGGCRRQMAQIRELVELPLRHP-QLFKSI-GIK 256 Query: 767 SXXAFLHVWGXLVPXKHPIARAVAXWKXGAFFLFN 871 L ++G K +ARAVA FFL N Sbjct: 257 PPRGIL-MYGPPGTGKTLMARAVANETGAFFFLIN 290 Score = 28.3 bits (60), Expect = 1.6 Identities = 14/36 (38%), Positives = 21/36 (58%) Frame = +1 Query: 223 LAQGQTPQGNRCIVLSDDNCPDEKIRMNRVVXNXLR 330 + +G+ + IVL+D+ D R+NRVV N LR Sbjct: 78 VVKGKRRKDTVLIVLTDEEMEDGVARINRVVRNNLR 113 >SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schizosaccharomyces pombe|chr 1|||Manual Length = 319 Score = 28.3 bits (60), Expect = 1.6 Identities = 12/39 (30%), Positives = 20/39 (51%), Gaps = 1/39 (2%) Frame = +3 Query: 78 LMIYRPRSSVARTDPTVSLSKKQSAMTTQSWH-FHRPKW 191 L + R + DP ++ + Q + T+SWH FH +W Sbjct: 259 LALIRGCDGLLSDDPVMARALSQGPIVTKSWHYFHYSEW 297 >SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 382 Score = 27.1 bits (57), Expect = 3.6 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 2/62 (3%) Frame = +3 Query: 27 FLDSNNKF*IKWQIIKALMIYRPRSSVARTDPTVS--LSKKQSAMTTQSWHFHRPKWSNF 200 F+++N K KW++ A+ P S + + PT+S + + SA TT +RP S Sbjct: 286 FIEANIK---KWRLAPAVGSLAPPPSYSTSQPTISWDAASESSAGTTTQGDTNRPDKSKN 342 Query: 201 NS 206 S Sbjct: 343 RS 344 >SPAC1556.02c |sdh1||succinate dehydrogenase Sdh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 641 Score = 26.6 bits (56), Expect = 4.8 Identities = 18/56 (32%), Positives = 26/56 (46%), Gaps = 1/56 (1%) Frame = -2 Query: 549 DHFELDGAHAPAD-QKVSSRWIGR*AXMKYGFKYTSNRLPVRPSXESSIGSMWTRF 385 DH L +H PA+ K I A + G T +PV P+ ++G + TRF Sbjct: 355 DHCYLQLSHLPAEILKERLPGISETAAIFAGVDVTKEPIPVLPTVHYNMGGIPTRF 410 >SPAC144.07c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 315 Score = 26.2 bits (55), Expect = 6.4 Identities = 8/14 (57%), Positives = 12/14 (85%) Frame = +1 Query: 790 MGPPGTGKTPHCXG 831 +GPPG+GK+ +C G Sbjct: 8 VGPPGSGKSTYCFG 21 >SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |Schizosaccharomyces pombe|chr 2|||Manual Length = 1944 Score = 26.2 bits (55), Expect = 6.4 Identities = 11/23 (47%), Positives = 13/23 (56%) Frame = +1 Query: 763 SLQXGISPCMGPPGTGKTPHCXG 831 S+ G + GPPGTGKT G Sbjct: 1283 SVNDGFTLIQGPPGTGKTKTILG 1305 >SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit Rpt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 438 Score = 26.2 bits (55), Expect = 6.4 Identities = 11/23 (47%), Positives = 11/23 (47%) Frame = +1 Query: 793 GPPGTGKTPHCXGCCXMETXGFL 861 GPPGTGKT C FL Sbjct: 226 GPPGTGKTLLARACAAQSNATFL 248 >SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 452 Score = 25.8 bits (54), Expect = 8.4 Identities = 14/47 (29%), Positives = 25/47 (53%) Frame = +3 Query: 99 SSVARTDPTVSLSKKQSAMTTQSWHFHRPKWSNFNSSVVTQSCSRAN 239 SSV S S ++M++ + +F+ S+ +SSV T + S +N Sbjct: 329 SSVVIVQSETSCSINSASMSSNTTYFYWNSTSSLSSSVFTNTTSSSN 375 >SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 409 Score = 25.8 bits (54), Expect = 8.4 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 1/27 (3%) Frame = +2 Query: 77 PD-DLSTAILRRKDRPNRLIVEEAVSD 154 PD D + + + RK+ PNRL++ A+S+ Sbjct: 233 PDADANISKIARKEDPNRLVLASAISE 259 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 8.4 Identities = 14/53 (26%), Positives = 30/53 (56%) Frame = +2 Query: 50 LNKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLF 208 L +A N + L+T +RK + N ++ +A ++ N+ +++ A E+ QL+ Sbjct: 768 LGSLAGNLPQESLTTKS-KRKSKVNLELMFDATAEKNNENSMNSAVFEKSQLY 819 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,356,564 Number of Sequences: 5004 Number of extensions: 61319 Number of successful extensions: 204 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 190 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 202 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 456499320 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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