Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30321.Seq
(904 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g09840.1 68416.m01174 cell division cycle protein 48 (CDC48A)... 211 4e-55
At5g03340.1 68418.m00286 cell division cycle protein 48, putativ... 209 2e-54
At3g53230.1 68416.m05865 cell division cycle protein 48, putativ... 194 5e-50
At4g13885.1 68417.m02151 3'-5' exonuclease-related contains weak... 31 1.4
At2g38770.1 68415.m04760 expressed protein 28 7.4
At5g22370.1 68418.m02610 ATP-binding family protein contains Pfa... 28 9.7
At1g53780.1 68414.m06120 26S proteasome AAA-ATPase subunit, puta... 28 9.7
>At3g09840.1 68416.m01174 cell division cycle protein 48 (CDC48A)
(CDC48) identical to SP|P54609 Cell division cycle
protein 48 homolog {Arabidopsis thaliana}
Length = 809
Score = 211 bits (516), Expect = 4e-55
Identities = 124/267 (46%), Positives = 158/267 (59%)
Frame = +2
Query: 62 ADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKR 241
+D+KS D STAIL RK PNRL+V+EA++DDNSVV+L A ME+LQLFRGDT+L+KGK+
Sbjct: 8 SDSKSKKDFSTAILERKKSPNRLVVDEAINDDNSVVSLHPATMEKLQLFRGDTILIKGKK 67
Query: 242 RKETVASCSQTIIALMRKFG*TVLXXTNFGXXXPXGEPXVXGPSVKYGKRVHILPIXDSX 421
RK+TV K + +N P VKYGKRVHILP+ D+
Sbjct: 68 RKDTVCIALADETCEEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGKRVHILPVDDTV 127
Query: 422 EGLTGNLFEVYLKPYFMXAYRPIHRDDTFWSAGACAPSSSKWSKQIHHHFASWAPDTVIH 601
EG+TGNLF+ YLKPYF+ AYRP+ + D F G K + + APDT I
Sbjct: 128 EGVTGNLFDAYLKPYFLEAYRPVRKGDLFLVRGGMRSVEFKVIETDPAEYCVVAPDTEIF 187
Query: 602 CDGEPIKREEEEEALNAVGYDDIGGCRKQLAQIXGMGGXATXSXLHXSKAIXGEASXXAF 781
C+GEP+KRE+EE L+ VGYDD+GG RKQ+AQI + K+I G
Sbjct: 188 CEGEPVKREDEER-LDDVGYDDVGGVRKQMAQIRELVELPLRHP-QLFKSI-GVKPPKGI 244
Query: 782 LHVWGXLVPXKHPIARAVAXWKXGAFF 862
L ++G K IARAVA + GAFF
Sbjct: 245 L-LYGPPGSGKTLIARAVAN-ETGAFF 269
>At5g03340.1 68418.m00286 cell division cycle protein 48, putative /
CDC48, putative very strong similarity to SP|P54609 Cell
division cycle protein 48 homolog {Arabidopsis
thaliana}; contains Pfam profiles PF00004: ATPase AAA
family, PF02359: Cell division protein 48 (CDC48)
N-terminal domain; supporting cDNA
gi|26449351|dbj|AK117125.1|
Length = 810
Score = 209 bits (510), Expect = 2e-54
Identities = 122/267 (45%), Positives = 157/267 (58%)
Frame = +2
Query: 62 ADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKR 241
+D+K+ D STAIL RK PNRL+V+EA++DDNSVV+L ME+LQLFRGDT+L+KGK+
Sbjct: 8 SDSKTKKDFSTAILERKKSPNRLVVDEAINDDNSVVSLHPTTMEKLQLFRGDTILIKGKK 67
Query: 242 RKETVASCSQTIIALMRKFG*TVLXXTNFGXXXPXGEPXVXGPSVKYGKRVHILPIXDSX 421
RK+TV K + +N P VKYGKRVHILP+ D+
Sbjct: 68 RKDTVCIALADETCEEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGKRVHILPVDDTV 127
Query: 422 EGLTGNLFEVYLKPYFMXAYRPIHRDDTFWSAGACAPSSSKWSKQIHHHFASWAPDTVIH 601
EG+TGNLF+ YLKPYF+ AYRP+ + D F G K + + APDT I
Sbjct: 128 EGVTGNLFDAYLKPYFLEAYRPVRKGDLFLVRGGMRSVEFKVIETDPAEYCVVAPDTEIF 187
Query: 602 CDGEPIKREEEEEALNAVGYDDIGGCRKQLAQIXGMGGXATXSXLHXSKAIXGEASXXAF 781
C+GEP+KRE+EE L+ VGYDD+GG RKQ+AQI + K+I G
Sbjct: 188 CEGEPVKREDEER-LDEVGYDDVGGVRKQMAQIRELVELPLRHP-QLFKSI-GVKPPKGI 244
Query: 782 LHVWGXLVPXKHPIARAVAXWKXGAFF 862
L ++G K IARAVA + GAFF
Sbjct: 245 L-LYGPPGSGKTLIARAVAN-ETGAFF 269
>At3g53230.1 68416.m05865 cell division cycle protein 48, putative /
CDC48, putative very strong similarity to SP|P54609 Cell
division cycle protein 48 homolog {Arabidopsis
thaliana}; contains Pfam profiles PF00004: ATPase AAA
family, PF02359: Cell division protein 48 (CDC48)
N-terminal domain
Length = 815
Score = 194 bits (474), Expect = 5e-50
Identities = 118/268 (44%), Positives = 155/268 (57%), Gaps = 1/268 (0%)
Frame = +2
Query: 62 ADNK-SPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGK 238
+D+K + D STAIL +K NRL+V+EA++DDNSVV+L ME+LQLFRGDT+L+KGK
Sbjct: 8 SDSKGTKKDFSTAILEKKKAANRLVVDEAINDDNSVVSLHPDTMEKLQLFRGDTILIKGK 67
Query: 239 RRKETVASCSQTIIALMRKFG*TVLXXTNFGXXXPXGEPXVXGPSVKYGKRVHILPIXDS 418
+RK+TV K + +N P VKYG RVHILP+ D+
Sbjct: 68 KRKDTVCIALADETCDEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGNRVHILPLDDT 127
Query: 419 XEGLTGNLFEVYLKPYFMXAYRPIHRDDTFWSAGACAPSSSKWSKQIHHHFASWAPDTVI 598
EG++GN+F+ YLKPYF+ AYRP+ + D F G K + + APDT I
Sbjct: 128 IEGVSGNIFDAYLKPYFLEAYRPVRKGDLFLVRGGMRSIEFKVIETDPAEYCVVAPDTEI 187
Query: 599 HCDGEPIKREEEEEALNAVGYDDIGGCRKQLAQIXGMGGXATXSXLHXSKAIXGEASXXA 778
C+GEPIKRE+EE L+ VGYDD+GG RKQ+AQI + K+I G
Sbjct: 188 FCEGEPIKREDEER-LDEVGYDDVGGVRKQMAQIRELVELPLRHP-QLFKSI-GVKPPKG 244
Query: 779 FLHVWGXLVPXKHPIARAVAXWKXGAFF 862
L ++G K IARAVA + GAFF
Sbjct: 245 IL-LYGPPGSGKTLIARAVAN-ETGAFF 270
Score = 37.5 bits (83), Expect = 0.012
Identities = 16/36 (44%), Positives = 24/36 (66%)
Frame = +1
Query: 223 LAQGQTPQGNRCIVLSDDNCPDEKIRMNRVVXNXLR 330
L +G+ + CI L+D+ C + KIRMN+VV + LR
Sbjct: 63 LIKGKKRKDTVCIALADETCDEPKIRMNKVVRSNLR 98
>At4g13885.1 68417.m02151 3'-5' exonuclease-related contains weak
similarity to Pfam domain PF01612: 3'-5' exonuclease
Length = 263
Score = 30.7 bits (66), Expect = 1.4
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = -1
Query: 214 TTEELKLLHFGL*KCHD*VVIAD 146
TTEELK+ H+ L KC D +V+A+
Sbjct: 3 TTEELKISHYKLYKCFDFLVVAN 25
>At2g38770.1 68415.m04760 expressed protein
Length = 1509
Score = 28.3 bits (60), Expect = 7.4
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = +1
Query: 766 LQXGISPCMGPPGTGKT 816
+Q G++ +GPPGTGKT
Sbjct: 878 IQPGLTMVVGPPGTGKT 894
>At5g22370.1 68418.m02610 ATP-binding family protein contains Pfam
domain, PF03029: Conserved hypothetical ATP binding
protein
Length = 291
Score = 27.9 bits (59), Expect = 9.7
Identities = 9/14 (64%), Positives = 12/14 (85%)
Frame = +1
Query: 790 MGPPGTGKTPHCXG 831
+GPPG+GKT +C G
Sbjct: 8 IGPPGSGKTTYCNG 21
>At1g53780.1 68414.m06120 26S proteasome AAA-ATPase subunit,
putative similar to 26S proteasome AAA-ATPase subunit
RPT1 SP:Q41365 from [Spinacia oleracea]
Length = 464
Score = 27.9 bits (59), Expect = 9.7
Identities = 21/69 (30%), Positives = 32/69 (46%)
Frame = +2
Query: 659 YDDIGGCRKQLAQIXGMGGXATXSXLHXSKAIXGEASXXAFLHVWGXLVPXKHPIARAVA 838
Y DIGGC++Q+ +I + LH K + + +G K +ARAVA
Sbjct: 204 YSDIGGCKEQIEKIREV---VELPMLHPEKFVRLGIDPPKGVLCYGPPGSGKTLVARAVA 260
Query: 839 XWKXGAFFL 865
+ GA F+
Sbjct: 261 N-RTGACFI 268
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,215,326
Number of Sequences: 28952
Number of extensions: 346317
Number of successful extensions: 982
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 933
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 965
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2129873112
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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