BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30308.Seq (812 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2 |Schizo... 124 2e-29 SPAC24C9.12c |||glycine hydroxymethyltransferase |Schizosaccharo... 122 8e-29 SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual 27 4.2 SPAC3F10.17 |||ribosome biogenesis protein Ltv1|Schizosaccharomy... 26 5.5 SPAC869.08 |pcm2||protein-L-isoaspartate O-methyltransferase |Sc... 26 7.3 SPBC11C11.05 |||KRE9 family cell wall biosynthesis protein |Schi... 26 7.3 SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy... 25 9.7 SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 25 9.7 >SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 472 Score = 124 bits (298), Expect = 2e-29 Identities = 53/82 (64%), Positives = 64/82 (78%) Frame = +1 Query: 259 RYYGGNEYIDEIEILAQNRSLEAYRLKSEEWGVNVQPYSGSPANFAVYTGIVEPHGRIMG 438 RYYGGNE+ID+ E L Q R+LEA+ L E+WGVNVQP+SGSPAN Y +++PH R+MG Sbjct: 73 RYYGGNEFIDQAERLCQTRALEAFHLDGEKWGVNVQPHSGSPANLQAYQAVMKPHDRLMG 132 Query: 439 LDLPDGGHLTHGFFTATKKISA 504 LDLP GGHL+HGF T K ISA Sbjct: 133 LDLPHGGHLSHGFSTPQKAISA 154 Score = 64.5 bits (150), Expect = 2e-11 Identities = 32/66 (48%), Positives = 41/66 (62%) Frame = +2 Query: 77 KLLNSNLWEADPELFDIIVKEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPI 256 KLL + L E DP ++ I+ EK RQ+ + +IASENFTS V+ L S + NKYSEG P Sbjct: 12 KLLKAPLAECDPTVYKILESEKSRQKESIALIASENFTSRAVMDALGSIMQNKYSEGYPG 71 Query: 257 NGTMGG 274 GG Sbjct: 72 ARYYGG 77 Score = 50.8 bits (116), Expect = 2e-07 Identities = 29/78 (37%), Positives = 45/78 (57%) Frame = +3 Query: 513 FFESMPYKVDPKSGLIDYDKLAETAKLFKPRLIIAGHELFTQDVSIYKXXREIRRRQXEL 692 +F +MPY V+ ++G+IDYD L + A F+P++I+AG + + V YK R+I Sbjct: 158 YFSTMPYNVNKETGIIDYDSLEKAAIQFRPKVIVAGASAYARLVD-YKRMRKI-TEMCNA 215 Query: 693 NLMADXGSRYLGLVAGQV 746 L+ D + GLVA V Sbjct: 216 YLLCDM-AHISGLVAAGV 232 >SPAC24C9.12c |||glycine hydroxymethyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 467 Score = 122 bits (293), Expect = 8e-29 Identities = 54/82 (65%), Positives = 64/82 (78%) Frame = +1 Query: 259 RYYGGNEYIDEIEILAQNRSLEAYRLKSEEWGVNVQPYSGSPANFAVYTGIVEPHGRIMG 438 RYYGGN++ID+IE L Q R+L A+ L +WGVNVQ SGSPAN VY I+ PHGR+MG Sbjct: 67 RYYGGNKFIDQIETLCQERALAAFNLDPAKWGVNVQCLSGSPANMQVYQAIMPPHGRLMG 126 Query: 439 LDLPDGGHLTHGFFTATKKISA 504 LDLP GGHL+HG+ T TKKISA Sbjct: 127 LDLPSGGHLSHGYQTDTKKISA 148 Score = 59.3 bits (137), Expect = 6e-10 Identities = 25/53 (47%), Positives = 38/53 (71%) Frame = +3 Query: 513 FFESMPYKVDPKSGLIDYDKLAETAKLFKPRLIIAGHELFTQDVSIYKXXREI 671 +FESMPY+VDP +GLIDYD L A+LF+P++++AG + + + Y R+I Sbjct: 152 YFESMPYRVDPNTGLIDYDMLEHDAQLFRPKILVAGTSAYCRLID-YARMRQI 203 Score = 58.0 bits (134), Expect = 1e-09 Identities = 30/60 (50%), Positives = 38/60 (63%) Frame = +2 Query: 95 LWEADPELFDIIVKEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPINGTMGG 274 L E DP + +I+ E DRQR+ + +IASENFTS V+ L S + NKYSEG P GG Sbjct: 12 LKEQDPTVAEIMRHEADRQRSSVVLIASENFTSRAVMDALGSVMSNKYSEGYPGARYYGG 71 >SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual Length = 510 Score = 26.6 bits (56), Expect = 4.2 Identities = 15/35 (42%), Positives = 21/35 (60%) Frame = -3 Query: 174 AIISSPAR*RSFSLTIISKSSGSASQRLLFSNLAL 70 +I+ S + S LT + KSSG AS + FSN +L Sbjct: 397 SILQSDSLMISTQLTSVQKSSGFASYSVQFSNCSL 431 >SPAC3F10.17 |||ribosome biogenesis protein Ltv1|Schizosaccharomyces pombe|chr 1|||Manual Length = 386 Score = 26.2 bits (55), Expect = 5.5 Identities = 16/58 (27%), Positives = 26/58 (44%), Gaps = 6/58 (10%) Frame = +1 Query: 247 HAHQR--YYGGNEYIDEIEILAQNRSLEAYRLK----SEEWGVNVQPYSGSPANFAVY 402 H QR Y + + + A+ + A RL EE+G ++P G AN+ +Y Sbjct: 19 HRSQRDPQYHDENATERVLVSAETLNKTARRLNRQTLDEEYGSTIRPNEGEAANYGIY 76 >SPAC869.08 |pcm2||protein-L-isoaspartate O-methyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 230 Score = 25.8 bits (54), Expect = 7.3 Identities = 12/49 (24%), Positives = 22/49 (44%) Frame = +1 Query: 277 EYIDEIEILAQNRSLEAYRLKSEEWGVNVQPYSGSPANFAVYTGIVEPH 423 +++ E + L R+++A S + + PY SP + I PH Sbjct: 15 QHLVESKFLTNQRAIKAMNATSRSFYCPLSPYMDSPQSIGYGVTISAPH 63 >SPBC11C11.05 |||KRE9 family cell wall biosynthesis protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 284 Score = 25.8 bits (54), Expect = 7.3 Identities = 13/56 (23%), Positives = 24/56 (42%), Gaps = 2/56 (3%) Frame = +1 Query: 250 AHQRYYGG--NEYIDEIEILAQNRSLEAYRLKSEEWGVNVQPYSGSPANFAVYTGI 411 A Y GG N+Y D + + + Y + + + V PY + F+ + G+ Sbjct: 108 AQSTYAGGIVNDYTDFFTVNGLTGTFDNYEIYASLMALGVYPYVPTLTGFSTFLGV 163 >SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1010 Score = 25.4 bits (53), Expect = 9.7 Identities = 15/58 (25%), Positives = 29/58 (50%) Frame = +2 Query: 68 MSAKLLNSNLWEADPELFDIIVKEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYS 241 ++ + LN L E D + + +KD + G ++ +EN +L LS+ +N +S Sbjct: 84 ITCQALNITLSETDSSKYYLEGFKKDLEEEGSPLLFNENNVDSALLSRLSTTGNNTFS 141 >SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosaccharomyces pombe|chr 2|||Manual Length = 2352 Score = 25.4 bits (53), Expect = 9.7 Identities = 18/56 (32%), Positives = 27/56 (48%), Gaps = 2/56 (3%) Frame = +1 Query: 256 QRYYG-GNEY-IDEIEILAQNRSLEAYRLKSEEWGVNVQPYSGSPANFAVYTGIVE 417 Q Y G N++ I E+ + N+S +A + +G PYS NF+ Y VE Sbjct: 1780 QSYEGDSNDFGIREVPLSDANQSSQADSTSIDRYG----PYSSQKVNFSKYKDFVE 1831 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,340,910 Number of Sequences: 5004 Number of extensions: 71365 Number of successful extensions: 189 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 173 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 189 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 396433620 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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