Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30296.Seq
(753 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac... 96 5e-21
SPAC1556.02c |sdh1||succinate dehydrogenase Sdh1|Schizosaccharom... 30 0.31
SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein S15a... 29 0.94
SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein S... 29 0.94
SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schi... 28 1.2
SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac... 28 1.6
SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr... 27 2.9
SPBP4G3.03 |||PI31 proteasome regulator related|Schizosaccharomy... 26 5.0
SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 6.6
SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr ... 26 6.6
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.6
SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyce... 25 8.8
>SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase
Cdc48|Schizosaccharomyces pombe|chr 1|||Manual
Length = 815
Score = 95.9 bits (228), Expect = 5e-21
Identities = 43/100 (43%), Positives = 63/100 (63%)
Frame = +1
Query: 223 LAQGQTPQGNRCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 402
+ +G+ + IVL+D+ D R+ D+V+I PCP +KY +R+ +L
Sbjct: 78 VVKGKRRKDTVLIVLTDEEMEDGVARINRVVRNNLRVRLGDIVTINPCPDIKYAERISVL 137
Query: 403 PIDDSVEGLTGNLFEVYLKPYFMEAYRPIHRDDTFRSAGA 522
P+ D+VEGLTG+LF+VYLKPYF+EAYRPI + D F G+
Sbjct: 138 PLADTVEGLTGSLFDVYLKPYFVEAYRPIRKGDLFVVRGS 177
Score = 88.6 bits (210), Expect = 8e-19
Identities = 41/68 (60%), Positives = 53/68 (77%)
Frame = +2
Query: 53 NKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLK 232
N D S +D +TAILR+K +PN L+V++A +DDNSV+ LS ME LQLFRGDTV++K
Sbjct: 21 NPPKDTYSAEDTATAILRKKRKPNSLVVDDATNDDNSVITLSSNTMETLQLFRGDTVVVK 80
Query: 233 GKRRKETV 256
GKRRK+TV
Sbjct: 81 GKRRKDTV 88
Score = 46.8 bits (106), Expect = 3e-06
Identities = 21/34 (61%), Positives = 25/34 (73%)
Frame = +3
Query: 510 VRGGMRAVEFKVVETDPSPFCIVAPDTVIHCDGE 611
VRG MR VEFKVV+ P F IV+ DT+IH +GE
Sbjct: 174 VRGSMRQVEFKVVDVAPDEFGIVSQDTIIHWEGE 207
>SPAC1556.02c |sdh1||succinate dehydrogenase
Sdh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 641
Score = 30.3 bits (65), Expect = 0.31
Identities = 19/56 (33%), Positives = 28/56 (50%), Gaps = 1/56 (1%)
Frame = -2
Query: 548 DHFELDGAHAPAD-LKVSSRWIGR*ASMKYGFKYTSNRLPVRPSTESSIGSMWTRF 384
DH L +H PA+ LK I A++ G T +PV P+ ++G + TRF
Sbjct: 355 DHCYLQLSHLPAEILKERLPGISETAAIFAGVDVTKEPIPVLPTVHYNMGGIPTRF 410
>SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein
S15a|Schizosaccharomyces pombe|chr 1|||Manual
Length = 130
Score = 28.7 bits (61), Expect = 0.94
Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%)
Frame = -3
Query: 421 QLNHQLAVCGLV---FHISLKDTERWI 350
QLN ++ CG++ F++ LKD E+W+
Sbjct: 64 QLNGRINKCGVISPRFNVKLKDIEKWV 90
>SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein
S15a|Schizosaccharomyces pombe|chr 1|||Manual
Length = 130
Score = 28.7 bits (61), Expect = 0.94
Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%)
Frame = -3
Query: 421 QLNHQLAVCGLV---FHISLKDTERWI 350
QLN ++ CG++ F++ LKD E+W+
Sbjct: 64 QLNGRINKCGVISPRFNVKLKDIEKWV 90
>SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 319
Score = 28.3 bits (60), Expect = 1.2
Identities = 12/39 (30%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Frame = +3
Query: 78 LMIYRPRSSVARTDPTVSLSKKQSAMTTQSWH-FHRPKW 191
L + R + DP ++ + Q + T+SWH FH +W
Sbjct: 259 LALIRGCDGLLSDDPVMARALSQGPIVTKSWHYFHYSEW 297
>SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase
E3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 905
Score = 27.9 bits (59), Expect = 1.6
Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
Frame = -3
Query: 391 LVFHISLKDTERWIP-HQKDAHEGCFSQHGSSEFSHQG 281
LV + LKD WI + + E F G ++FS+QG
Sbjct: 435 LVSDLYLKDLWSWIHLSHRQSEESLFGDTGDTDFSYQG 472
>SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 382
Score = 27.1 bits (57), Expect = 2.9
Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 2/62 (3%)
Frame = +3
Query: 27 FLDSNNKF*IKWQIIKALMIYRPRSSVARTDPTVS--LSKKQSAMTTQSWHFHRPKWSNF 200
F+++N K KW++ A+ P S + + PT+S + + SA TT +RP S
Sbjct: 286 FIEANIK---KWRLAPAVGSLAPPPSYSTSQPTISWDAASESSAGTTTQGDTNRPDKSKN 342
Query: 201 NS 206
S
Sbjct: 343 RS 344
>SPBP4G3.03 |||PI31 proteasome regulator related|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 241
Score = 26.2 bits (55), Expect = 5.0
Identities = 14/48 (29%), Positives = 21/48 (43%)
Frame = -3
Query: 391 LVFHISLKDTERWIPHQKDAHEGCFSQHGSSEFSHQGNYRLRARCNGF 248
L FH ++ D W PH+ + G F H + +G + R GF
Sbjct: 177 LGFHPNVGDYFDWYPHRPNHFGGKFDGHSRGRGAGRGGFCGRHGRGGF 224
>SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 452
Score = 25.8 bits (54), Expect = 6.6
Identities = 14/47 (29%), Positives = 25/47 (53%)
Frame = +3
Query: 99 SSVARTDPTVSLSKKQSAMTTQSWHFHRPKWSNFNSSVVTQSCSRAN 239
SSV S S ++M++ + +F+ S+ +SSV T + S +N
Sbjct: 329 SSVVIVQSETSCSINSASMSSNTTYFYWNSTSSLSSSVFTNTTSSSN 375
>SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 409
Score = 25.8 bits (54), Expect = 6.6
Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 1/27 (3%)
Frame = +2
Query: 77 PD-DLSTAILRRKDRPNRLIVEEAVSD 154
PD D + + + RK+ PNRL++ A+S+
Sbjct: 233 PDADANISKIARKEDPNRLVLASAISE 259
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.6
Identities = 14/53 (26%), Positives = 30/53 (56%)
Frame = +2
Query: 50 LNKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLF 208
L +A N + L+T +RK + N ++ +A ++ N+ +++ A E+ QL+
Sbjct: 768 LGSLAGNLPQESLTTKS-KRKSKVNLELMFDATAEKNNENSMNSAVFEKSQLY 819
>SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1008
Score = 25.4 bits (53), Expect = 8.8
Identities = 10/38 (26%), Positives = 22/38 (57%)
Frame = +2
Query: 137 EEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKRRKE 250
E+AV D+N+ ++L+ + + RGD ++ G ++
Sbjct: 247 EKAVCDENTKISLTNTEHYKFHSLRGDVEVVVGDLERD 284
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,048,120
Number of Sequences: 5004
Number of extensions: 59541
Number of successful extensions: 177
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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