BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30289.Seq (648 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC31H12.04c |rpl1202|rpl12-2|60S ribosomal protein L12.1/L12A|... 108 6e-25 SPCC16C4.13c |rpl1201|rpl12-1, rpl12.1|60S ribosomal protein L12... 108 6e-25 SPBC365.11 |||GRIP domain protein|Schizosaccharomyces pombe|chr ... 26 4.1 SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3 |Schiz... 26 5.4 SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynei... 26 5.4 SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 25 7.1 SPCC5E4.10c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 25 7.1 SPAC1783.05 |hrp1|chd1|ATP-dependent DNA helicase Hrp1|Schizosac... 25 9.4 SPBC725.14 |arg6||acetylglutamate synthase Arg6 |Schizosaccharom... 25 9.4 >SPCC31H12.04c |rpl1202|rpl12-2|60S ribosomal protein L12.1/L12A|Schizosaccharomyces pombe|chr 3|||Manual Length = 165 Score = 108 bits (260), Expect = 6e-25 Identities = 54/106 (50%), Positives = 76/106 (71%), Gaps = 5/106 (4%) Frame = +3 Query: 252 KDWKGLKITVQLTVQNRQAQIAVVPSAAALIIRALKEPPRDRKKQKNIKHNGNISLEDVI 431 KDWKGL++TV+LT+QNRQA ++VVPSA+AL+I+ALKEP RDRKK KN+ H+GN+SL+++I Sbjct: 51 KDWKGLRVTVKLTIQNRQAAVSVVPSASALVIKALKEPARDRKKDKNVAHSGNVSLDEII 110 Query: 432 G-----HCEDHEKQINGPVPFWLSKRDSWHRQSVGCTVEGRXAHDL 554 + K+++G V K SVGCTV+G+ HD+ Sbjct: 111 EVARTMRFKSLAKELSGTV-----KEILGTAFSVGCTVDGKNPHDV 151 Score = 99.5 bits (237), Expect = 4e-22 Identities = 58/135 (42%), Positives = 74/135 (54%) Frame = +1 Query: 103 MPPKFDPNEIKIVNLRCVGGEVGATSSLAPKIGPLGLSPKKVGDDIAKATRTGRVSRSLC 282 MPPKFDPNE+K + +R VGGEV S+LAPKIGPLGLSPKKVG+DIAKAT+ + R Sbjct: 1 MPPKFDPNEVKTIFMRAVGGEVAGGSTLAPKIGPLGLSPKKVGEDIAKATKDWKGLRVTV 60 Query: 283 S*QFKTDKXXXXXXXXXXXXXXXXXXXXXVTVKSRKISNTTATSPLKM*SGIAKIMRNRS 462 + + K K + L +A+ MR +S Sbjct: 61 KLTIQNRQAAVSVVPSASALVIKALKEPARDRKKDKNVAHSGNVSLDEIIEVARTMRFKS 120 Query: 463 MARYLSGSVKEILGT 507 +A+ LSG+VKEILGT Sbjct: 121 LAKELSGTVKEILGT 135 >SPCC16C4.13c |rpl1201|rpl12-1, rpl12.1|60S ribosomal protein L12.1/L12A|Schizosaccharomyces pombe|chr 3|||Manual Length = 165 Score = 108 bits (260), Expect = 6e-25 Identities = 54/106 (50%), Positives = 76/106 (71%), Gaps = 5/106 (4%) Frame = +3 Query: 252 KDWKGLKITVQLTVQNRQAQIAVVPSAAALIIRALKEPPRDRKKQKNIKHNGNISLEDVI 431 KDWKGL++TV+LT+QNRQA ++VVPSA+AL+I+ALKEP RDRKK KN+ H+GN+SL+++I Sbjct: 51 KDWKGLRVTVKLTIQNRQAAVSVVPSASALVIKALKEPARDRKKDKNVAHSGNVSLDEII 110 Query: 432 G-----HCEDHEKQINGPVPFWLSKRDSWHRQSVGCTVEGRXAHDL 554 + K+++G V K SVGCTV+G+ HD+ Sbjct: 111 EVARTMRFKSLAKELSGTV-----KEILGTAFSVGCTVDGKNPHDV 151 Score = 99.5 bits (237), Expect = 4e-22 Identities = 58/135 (42%), Positives = 74/135 (54%) Frame = +1 Query: 103 MPPKFDPNEIKIVNLRCVGGEVGATSSLAPKIGPLGLSPKKVGDDIAKATRTGRVSRSLC 282 MPPKFDPNE+K + +R VGGEV S+LAPKIGPLGLSPKKVG+DIAKAT+ + R Sbjct: 1 MPPKFDPNEVKTIFMRAVGGEVAGGSTLAPKIGPLGLSPKKVGEDIAKATKDWKGLRVTV 60 Query: 283 S*QFKTDKXXXXXXXXXXXXXXXXXXXXXVTVKSRKISNTTATSPLKM*SGIAKIMRNRS 462 + + K K + L +A+ MR +S Sbjct: 61 KLTIQNRQAAVSVVPSASALVIKALKEPARDRKKDKNVAHSGNVSLDEIIEVARTMRFKS 120 Query: 463 MARYLSGSVKEILGT 507 +A+ LSG+VKEILGT Sbjct: 121 LAKELSGTVKEILGT 135 >SPBC365.11 |||GRIP domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 266 Score = 26.2 bits (55), Expect = 4.1 Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%) Frame = +3 Query: 351 ALKEPPRDRKKQKN-IKHNGNISLEDVIGHCEDHEKQINGPV 473 A+ +PP +KK+KN K N E +G+ D E+Q +G + Sbjct: 30 AITKPPASKKKRKNRKKKKNNGPSEQFVGN-NDLEEQRSGSI 70 >SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 900 Score = 25.8 bits (54), Expect = 5.4 Identities = 12/29 (41%), Positives = 16/29 (55%) Frame = +3 Query: 420 EDVIGHCEDHEKQINGPVPFWLSKRDSWH 506 E+++G C DHEK +WL DS H Sbjct: 345 EEILGLCCDHEKN-----TYWLYTTDSLH 368 >SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynein Mcp5/Num1|Schizosaccharomyces pombe|chr 2|||Manual Length = 968 Score = 25.8 bits (54), Expect = 5.4 Identities = 13/36 (36%), Positives = 18/36 (50%) Frame = -2 Query: 383 LFTVTRRLLKGSDDKGCCRGNNSYLGLSVLNCQLHS 276 +FT+ R LK + CR N + GLS C +S Sbjct: 805 VFTLPRNNLKSKTNTKKCRDNLNLSGLSSSTCNANS 840 >SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pombe|chr 2|||Manual Length = 1067 Score = 25.4 bits (53), Expect = 7.1 Identities = 11/34 (32%), Positives = 17/34 (50%) Frame = -2 Query: 269 ETLPVLVALAMSSPTFLGDRPRGPILGAKDDVAP 168 E +PV ++ SP L D R +L ++AP Sbjct: 1017 ELIPVFASVLTGSPEQLNDELRSELLSMVKEIAP 1050 >SPCC5E4.10c |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 101 Score = 25.4 bits (53), Expect = 7.1 Identities = 11/39 (28%), Positives = 18/39 (46%) Frame = +3 Query: 348 RALKEPPRDRKKQKNIKHNGNISLEDVIGHCEDHEKQIN 464 R K P DR++Q K + I D + H H +++ Sbjct: 50 RKTKNPESDRRRQSRKKKSTQIQASDEMKHRRHHVHKVH 88 >SPAC1783.05 |hrp1|chd1|ATP-dependent DNA helicase Hrp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1373 Score = 25.0 bits (52), Expect = 9.4 Identities = 9/25 (36%), Positives = 16/25 (64%) Frame = +3 Query: 378 KKQKNIKHNGNISLEDVIGHCEDHE 452 K +N K N++L+D++ H ED + Sbjct: 887 KATENQKKLENMNLDDILSHAEDRD 911 >SPBC725.14 |arg6||acetylglutamate synthase Arg6 |Schizosaccharomyces pombe|chr 2|||Manual Length = 500 Score = 25.0 bits (52), Expect = 9.4 Identities = 10/18 (55%), Positives = 13/18 (72%) Frame = -2 Query: 182 DDVAPTSPPTHRKFTILI 129 D++A T PP HRK IL+ Sbjct: 249 DELAKTLPPYHRKNLILV 266 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,663,429 Number of Sequences: 5004 Number of extensions: 55431 Number of successful extensions: 158 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 154 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 291768710 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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