BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30258.Seq (721 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC2G11.03c |vps45||vacuolar sorting protein Vps 45|Schizosacch... 77 3e-15 SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces ... 34 0.023 SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni... 31 0.13 SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 29 0.51 SPBC25H2.09 |||DUF1690 family protein|Schizosaccharomyces pombe|... 26 6.2 >SPAC2G11.03c |vps45||vacuolar sorting protein Vps 45|Schizosaccharomyces pombe|chr 1|||Manual Length = 558 Score = 76.6 bits (180), Expect = 3e-15 Identities = 33/68 (48%), Positives = 45/68 (66%) Frame = +3 Query: 255 HMKCIVFIRPTSENIALLSRELRDPKYGVYFIYFSNVVSKADIKTLAXCDXXEAVRXVQX 434 H+KC+ F+RPT + LL ELRDPKY Y +YF+NV+ K+ ++ LA D EAV+ +Q Sbjct: 63 HLKCVAFLRPTPTTLRLLCEELRDPKYAEYHLYFTNVIPKSFLERLAESDDFEAVKSIQE 122 Query: 435 VFQDNLTV 458 F D L V Sbjct: 123 FFLDYLVV 130 Score = 36.7 bits (81), Expect = 0.003 Identities = 16/51 (31%), Positives = 33/51 (64%) Frame = +1 Query: 61 MNVIQAVKMYITKMTXXSGPGMKVILMDKETTSIVSMVYSQSEILQKEVYL 213 M+++ A + Y K +K++L++++TT IVS +QS +L++++YL Sbjct: 1 MDLVSASQSYF-KRIFQEVSDLKILLLEEDTTKIVSSCITQSNLLEQQIYL 50 >SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces pombe|chr 3|||Manual Length = 639 Score = 33.9 bits (74), Expect = 0.023 Identities = 15/43 (34%), Positives = 26/43 (60%) Frame = +3 Query: 267 IVFIRPTSENIALLSRELRDPKYGVYFIYFSNVVSKADIKTLA 395 I F++PT ENI L+ +L Y ++ FS+ +S+A ++ A Sbjct: 92 IYFVQPTQENIELIIEDLSKGLYESAYVCFSSTISRALLEQFA 134 >SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit Cct1|Schizosaccharomyces pombe|chr 2|||Manual Length = 556 Score = 31.5 bits (68), Expect = 0.13 Identities = 19/45 (42%), Positives = 26/45 (57%) Frame = +3 Query: 414 AVRXVQXVFQDNLTVTVESLGRAVSDSTQAKTPMXSKLIGADAGF 548 A+R D L+ +V+SLG+ S AKT M SK+IG D+ F Sbjct: 129 AIREAVKFMTDVLSCSVDSLGKE-SLINVAKTSMSSKIIGNDSDF 172 >SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 2685 Score = 29.5 bits (63), Expect = 0.51 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = -1 Query: 280 RIKTMHFMCSCYPIWHDCLSSRISKLPSVRFP 185 R++ +H + SC+P HD S + K P FP Sbjct: 430 RLELLHDVLSCFPKKHDSTSRKKPKFPYQYFP 461 >SPBC25H2.09 |||DUF1690 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 162 Score = 25.8 bits (54), Expect = 6.2 Identities = 14/42 (33%), Positives = 20/42 (47%) Frame = +3 Query: 213 IREDRQSCQMG*HEHMKCIVFIRPTSENIALLSRELRDPKYG 338 IR D C EH + P +E A+L+ +L +PK G Sbjct: 122 IRSDLLKCMS---EHPDKSLICHPLAEKFAILASKLHNPKVG 160 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,776,798 Number of Sequences: 5004 Number of extensions: 53449 Number of successful extensions: 98 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 94 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 98 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 337208592 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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