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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30258.Seq
         (721 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g77140.1 68414.m08986 vacuolar protein sorting protein 45, pu...    62   4e-10
At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE...    34   0.082
At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putat...    33   0.19 
At3g53510.1 68416.m05908 ABC transporter family protein breast c...    29   4.1  
At3g20050.1 68416.m02536 T-complex protein 1 alpha subunit / TCP...    28   7.2  
At3g11900.1 68416.m01459 amino acid transporter family protein l...    27   9.5  

>At1g77140.1 68414.m08986 vacuolar protein sorting protein 45,
           putative / VPS45p, putative identical to vacuolar
           protein sorting homolog VPS45p [Arabidopsis thaliana]
           gi|2921406|gb|AAC39472
          Length = 569

 Score = 62.1 bits (144), Expect = 4e-10
 Identities = 27/64 (42%), Positives = 40/64 (62%)
 Frame = +3

Query: 255 HMKCIVFIRPTSENIALLSRELRDPKYGVYFIYFSNVVSKADIKTLAXCDXXEAVRXVQX 434
           H+K + FIRPTS+NI  L  +L +P++G Y ++FSN++    I  LA  D  E V+ VQ 
Sbjct: 66  HLKAVYFIRPTSDNIQKLRYQLANPRFGEYHLFFSNLLKDTQIHILADSDEQEVVQQVQE 125

Query: 435 VFQD 446
            + D
Sbjct: 126 YYAD 129



 Score = 60.9 bits (141), Expect = 8e-10
 Identities = 31/57 (54%), Positives = 42/57 (73%)
 Frame = +1

Query: 61  MNVIQAVKMYITKMTXXSGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDS 231
           M ++ +V+ YI +M      GMKV+++D ET S VS+VYSQSE+LQKEV+L E IDS
Sbjct: 1   MVLVTSVRDYINRMLQDIS-GMKVLILDSETVSNVSIVYSQSELLQKEVFLVEMIDS 56


>At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE)
           similar to cytokinesis-related Sec1 protein KEULE
           [Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
           Pfam domain, PF00995: Sec1 family
          Length = 666

 Score = 34.3 bits (75), Expect = 0.082
 Identities = 16/41 (39%), Positives = 25/41 (60%), Gaps = 2/41 (4%)
 Frame = +3

Query: 258 MKCIVFIRPTSENIALLSREL--RDPKYGVYFIYFSNVVSK 374
           M  I FI+PT EN+ +   ++  + P Y   F++FS+ VSK
Sbjct: 86  MDAIYFIQPTKENVIMFLSDMSGKSPLYKKAFVFFSSPVSK 126


>At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putative
           similar to cytokinesis-related Sec1 protein KEULE
           [Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
           Pfam domain, PF00995: Sec1 family
          Length = 662

 Score = 33.1 bits (72), Expect = 0.19
 Identities = 15/42 (35%), Positives = 26/42 (61%), Gaps = 2/42 (4%)
 Frame = +3

Query: 258 MKCIVFIRPTSENIALLSREL--RDPKYGVYFIYFSNVVSKA 377
           M+ I FI+PT EN+     ++  + P Y   F++FS+ VS++
Sbjct: 86  MEVIYFIQPTEENVTAFLSDMTGKSPLYKKAFVFFSSPVSRS 127



 Score = 31.9 bits (69), Expect = 0.44
 Identities = 16/36 (44%), Positives = 21/36 (58%)
 Frame = +1

Query: 127 KVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSH 234
           KV++MDK T  I+S     SEI Q+ + L E I  H
Sbjct: 44  KVLVMDKFTVKIMSSACKMSEITQEGISLVEVITKH 79


>At3g53510.1 68416.m05908 ABC transporter family protein breast
           cancer resistance protein (BCRP), Homo sapiens,
           EMBL:AF098951
          Length = 739

 Score = 28.7 bits (61), Expect = 4.1
 Identities = 15/49 (30%), Positives = 27/49 (55%)
 Frame = -3

Query: 176 YTILTMLVVSLSINITFIPGPLSSVILVMYILTACITFILIKLSLFISQ 30
           +TILT      S  +TF+ G +S V++   ++ A + + L+    FIS+
Sbjct: 568 FTILTAFWAGSSF-VTFLSGVVSHVMIGFTVVVAILAYFLLFSGFFISR 615


>At3g20050.1 68416.m02536 T-complex protein 1 alpha subunit /
           TCP-1-alpha / chaperonin (CCT1) identical to
           SWISS-PROT:P28769- T-complex protein 1, alpha subunit
           (TCP-1-alpha) [Arabidopsis thaliana]
          Length = 545

 Score = 27.9 bits (59), Expect = 7.2
 Identities = 18/45 (40%), Positives = 23/45 (51%)
 Frame = +3

Query: 414 AVRXVQXVFQDNLTVTVESLGRAVSDSTQAKTPMXSKLIGADAGF 548
           A+R      ++ L   VE LG+ V     AKT M SKLI  D+ F
Sbjct: 127 AMRESCKYIEEKLVTKVEKLGK-VPLINCAKTSMSSKLISGDSDF 170


>At3g11900.1 68416.m01459 amino acid transporter family protein low
           similarity to proton/amino acid transporter 1 [Mus
           musculus] GI:21908024; belongs to INTERPRO:IPR002422
           amino acid/polyamine transporter, family II
          Length = 432

 Score = 27.5 bits (58), Expect = 9.5
 Identities = 15/41 (36%), Positives = 24/41 (58%)
 Frame = -3

Query: 179 LYTILTMLVVSLSINITFIPGPLSSVILVMYILTACITFIL 57
           ++T  T+LVV L+   + +PG  +   LV   L A I+F+L
Sbjct: 349 IFTTRTLLVVGLAAIASLVPGFGTFASLVGSTLCALISFVL 389


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,337,847
Number of Sequences: 28952
Number of extensions: 273360
Number of successful extensions: 488
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 473
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 487
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1565336320
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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