BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30256.Seq (603 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g77140.1 68414.m08986 vacuolar protein sorting protein 45, pu... 79 2e-15 At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putat... 37 0.012 At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE... 36 0.021 At3g15060.1 68416.m01905 Ras-related GTP-binding family protein ... 29 1.8 At1g28380.1 68414.m03487 expressed protein 29 2.4 At3g53510.1 68416.m05908 ABC transporter family protein breast c... 29 3.1 At5g14390.1 68418.m01681 expressed protein 28 4.1 At3g01690.1 68416.m00101 expressed protein 28 4.1 At4g24760.1 68417.m03545 expressed protein 28 5.5 At4g31020.2 68417.m04406 expressed protein 27 7.2 At4g31020.1 68417.m04405 expressed protein 27 7.2 At3g11900.1 68416.m01459 amino acid transporter family protein l... 27 7.2 At2g24320.1 68415.m02907 hypothetical protein 27 7.2 At1g74430.1 68414.m08623 myb family transcription factor (MYB95)... 27 7.2 At5g57990.1 68418.m07255 ubiquitin-specific protease 23, putativ... 27 9.6 At1g08440.1 68414.m00933 hypothetical protein contains Pfam prof... 27 9.6 >At1g77140.1 68414.m08986 vacuolar protein sorting protein 45, putative / VPS45p, putative identical to vacuolar protein sorting homolog VPS45p [Arabidopsis thaliana] gi|2921406|gb|AAC39472 Length = 569 Score = 79.0 bits (186), Expect = 2e-15 Identities = 33/77 (42%), Positives = 54/77 (70%) Frame = +3 Query: 255 HMKCIVFIRPTSENIALLSRELRDPKYGVYFIYFSNVVSKADIKTLAECDEXEAVREVQE 434 H+K + FIRPTS+NI L +L +P++G Y ++FSN++ I LA+ DE E V++VQE Sbjct: 66 HLKAVYFIRPTSDNIQKLRYQLANPRFGEYHLFFSNLLKDTQIHILADSDEQEVVQQVQE 125 Query: 435 VFADYLAVDRHLFSFNI 485 +AD+++ D + F+ N+ Sbjct: 126 YYADFVSGDPYHFTLNM 142 Score = 62.1 bits (144), Expect = 3e-10 Identities = 31/57 (54%), Positives = 43/57 (75%) Frame = +1 Query: 61 MNVIQAVKMYITKMTXESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDS 231 M ++ +V+ YI +M + GMKV+++D ET S VS+VYSQSE+LQKEV+L E IDS Sbjct: 1 MVLVTSVRDYINRMLQDIS-GMKVLILDSETVSNVSIVYSQSELLQKEVFLVEMIDS 56 >At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putative similar to cytokinesis-related Sec1 protein KEULE [Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains Pfam domain, PF00995: Sec1 family Length = 662 Score = 36.7 bits (81), Expect = 0.012 Identities = 21/84 (25%), Positives = 45/84 (53%), Gaps = 3/84 (3%) Frame = +3 Query: 258 MKCIVFIRPTSENIALLSREL--RDPKYGVYFIYFSNVVSKADIKTL-AECDEXEAVREV 428 M+ I FI+PT EN+ ++ + P Y F++FS+ VS++ + + + + + + Sbjct: 86 MEVIYFIQPTEENVTAFLSDMTGKSPLYKKAFVFFSSPVSRSLVNLIKKDMRAMKRIGGL 145 Query: 429 QEVFADYLAVDRHLFSFNIVGCLQ 500 +E+ +Y+++D F N L+ Sbjct: 146 KEMNLEYISMDIQGFVTNNENALE 169 Score = 31.9 bits (69), Expect = 0.34 Identities = 16/36 (44%), Positives = 21/36 (58%) Frame = +1 Query: 127 KVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSH 234 KV++MDK T I+S SEI Q+ + L E I H Sbjct: 44 KVLVMDKFTVKIMSSACKMSEITQEGISLVEVITKH 79 >At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE) similar to cytokinesis-related Sec1 protein KEULE [Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains Pfam domain, PF00995: Sec1 family Length = 666 Score = 35.9 bits (79), Expect = 0.021 Identities = 23/95 (24%), Positives = 45/95 (47%), Gaps = 3/95 (3%) Frame = +3 Query: 258 MKCIVFIRPTSENIALLSREL--RDPKYGVYFIYFSNVVSKADIKTL-AECDEXEAVREV 428 M I FI+PT EN+ + ++ + P Y F++FS+ VSK + + + + + Sbjct: 86 MDAIYFIQPTKENVIMFLSDMSGKSPLYKKAFVFFSSPVSKELVGHIKKDSSVLPRIGAL 145 Query: 429 QEVFADYLAVDRHLFSFNIVGCLQGRVGTNSISNE 533 +E+ ++ A+D F + L+ G S + Sbjct: 146 REMNLEFFAIDSQGFITDHERALEDLFGDEETSRK 180 >At3g15060.1 68416.m01905 Ras-related GTP-binding family protein similar to GTP-binding protein GI:303742 from [Pisum sativum]; contains Pfam profile: PF00071 ras family Length = 217 Score = 29.5 bits (63), Expect = 1.8 Identities = 27/83 (32%), Positives = 38/83 (45%), Gaps = 2/83 (2%) Frame = -2 Query: 584 RRLAASETGGAPADLEKLVGDAVGSNPTLEASN--NIEREQVSVHSQVVSKNFLDLSDSL 411 R L A T A A E+ LEA N N E +S +V SK LD+ D Sbjct: 131 RHLRAVSTEDAKAFAER-ENTFFMETSALEALNVENAFTEVLSQIYRVASKKALDIGDDH 189 Query: 410 XFITFGQSFDVCLRNYITEIYKV 342 + GQS +V ++ ++E+ KV Sbjct: 190 TTLPKGQSINVGSKDDVSEVKKV 212 >At1g28380.1 68414.m03487 expressed protein Length = 612 Score = 29.1 bits (62), Expect = 2.4 Identities = 23/93 (24%), Positives = 40/93 (43%), Gaps = 1/93 (1%) Frame = +3 Query: 324 DPKYGVYFIYFSNVVSKADIKTLAECDEXEAVREVQEVFADYLAVDRHLFSFNIVGCLQG 503 DP I+ +V ++ + +CD+ E R + +D L+ ++ FN CL G Sbjct: 50 DPTRNRDLIFPGGIVVN-NVSSSIKCDKGERTR----LRSDILSFNQMSEKFNQDMCLSG 104 Query: 504 RVGTNSISNEF-LKVCWRSSCL*SGEPAFAYEG 599 ++ + +N F CW S AY+G Sbjct: 105 KIPSGMFNNMFAFSKCWPKDA--SSVKTLAYDG 135 >At3g53510.1 68416.m05908 ABC transporter family protein breast cancer resistance protein (BCRP), Homo sapiens, EMBL:AF098951 Length = 739 Score = 28.7 bits (61), Expect = 3.1 Identities = 15/49 (30%), Positives = 27/49 (55%) Frame = -2 Query: 176 YTILTMLVVSLSINITFIPGPLSSVILVMYILTACITFILIKLSLFISQ 30 +TILT S +TF+ G +S V++ ++ A + + L+ FIS+ Sbjct: 568 FTILTAFWAGSSF-VTFLSGVVSHVMIGFTVVVAILAYFLLFSGFFISR 615 >At5g14390.1 68418.m01681 expressed protein Length = 369 Score = 28.3 bits (60), Expect = 4.1 Identities = 16/43 (37%), Positives = 26/43 (60%), Gaps = 1/43 (2%) Frame = -2 Query: 566 ETGGAPADLEKLVGDAVGSNPTLEASNNIEREQVSV-HSQVVS 441 ET GA + L G +VGS PTL+ + + + + +V HS ++S Sbjct: 132 ETYGAKQEDIILYGQSVGSGPTLDLAARLPQLRAAVLHSPILS 174 >At3g01690.1 68416.m00101 expressed protein Length = 361 Score = 28.3 bits (60), Expect = 4.1 Identities = 15/43 (34%), Positives = 26/43 (60%), Gaps = 1/43 (2%) Frame = -2 Query: 566 ETGGAPADLEKLVGDAVGSNPTLEASNNI-EREQVSVHSQVVS 441 ET G+ + L G +VGS PTL+ ++ + + V +HS ++S Sbjct: 132 ETFGSKQEGVILYGQSVGSGPTLDLASRLPQLRAVVLHSPILS 174 >At4g24760.1 68417.m03545 expressed protein Length = 365 Score = 27.9 bits (59), Expect = 5.5 Identities = 15/43 (34%), Positives = 25/43 (58%), Gaps = 1/43 (2%) Frame = -2 Query: 566 ETGGAPADLEKLVGDAVGSNPTLEASNNIEREQVSV-HSQVVS 441 E GA + L G +VGS PT++ + + R + S+ HS ++S Sbjct: 132 ENYGAKQENIILYGQSVGSGPTVDLAARLPRLRASILHSPILS 174 >At4g31020.2 68417.m04406 expressed protein Length = 294 Score = 27.5 bits (58), Expect = 7.2 Identities = 13/32 (40%), Positives = 21/32 (65%), Gaps = 1/32 (3%) Frame = -2 Query: 533 LVGDAVGSNPTLEASNNIER-EQVSVHSQVVS 441 L G +VGS PTL ++ ++R V +HS ++S Sbjct: 143 LYGQSVGSGPTLHMASRLKRLRGVVLHSAILS 174 >At4g31020.1 68417.m04405 expressed protein Length = 294 Score = 27.5 bits (58), Expect = 7.2 Identities = 13/32 (40%), Positives = 21/32 (65%), Gaps = 1/32 (3%) Frame = -2 Query: 533 LVGDAVGSNPTLEASNNIER-EQVSVHSQVVS 441 L G +VGS PTL ++ ++R V +HS ++S Sbjct: 143 LYGQSVGSGPTLHMASRLKRLRGVVLHSAILS 174 >At3g11900.1 68416.m01459 amino acid transporter family protein low similarity to proton/amino acid transporter 1 [Mus musculus] GI:21908024; belongs to INTERPRO:IPR002422 amino acid/polyamine transporter, family II Length = 432 Score = 27.5 bits (58), Expect = 7.2 Identities = 15/41 (36%), Positives = 24/41 (58%) Frame = -2 Query: 179 LYTILTMLVVSLSINITFIPGPLSSVILVMYILTACITFIL 57 ++T T+LVV L+ + +PG + LV L A I+F+L Sbjct: 349 IFTTRTLLVVGLAAIASLVPGFGTFASLVGSTLCALISFVL 389 >At2g24320.1 68415.m02907 hypothetical protein Length = 286 Score = 27.5 bits (58), Expect = 7.2 Identities = 12/32 (37%), Positives = 21/32 (65%), Gaps = 1/32 (3%) Frame = -2 Query: 533 LVGDAVGSNPTLEASNNIER-EQVSVHSQVVS 441 L G +VGS PTL ++ ++R + +HS ++S Sbjct: 135 LYGQSVGSGPTLHLASRVKRLRGIVLHSAILS 166 >At1g74430.1 68414.m08623 myb family transcription factor (MYB95) contains Pfam profile: PF00249 myb-like DNA-binding domain Length = 271 Score = 27.5 bits (58), Expect = 7.2 Identities = 23/71 (32%), Positives = 35/71 (49%) Frame = +1 Query: 391 WPNVMKXRLSERSRKFLLTTWLWTDTCSLSILLDASKVGLEPTASPTSFSRSAGAPPVSE 570 W + +K RL+++ + T T T SL++ + +S PT SPTS S S+ S Sbjct: 108 WNSCLKKRLAKKGIDPM--THEPTTTTSLTVDVTSSSTTSSPTPSPTSSSFSS----CSS 161 Query: 571 AASLRSLTRAA 603 S R L + A Sbjct: 162 TGSARFLNKLA 172 >At5g57990.1 68418.m07255 ubiquitin-specific protease 23, putative (UBP23) identical to GI:11993486 Length = 859 Score = 27.1 bits (57), Expect = 9.6 Identities = 17/57 (29%), Positives = 31/57 (54%) Frame = -2 Query: 599 ALVSERRLAASETGGAPADLEKLVGDAVGSNPTLEASNNIEREQVSVHSQVVSKNFL 429 +L++ ++ +S G +D ++ VGS+ EAS N +R++ SV Q + N L Sbjct: 715 SLIAPSKMISSSDGAVTSDQQQ----PVGSSDLSEASQNAKRKRESVLLQKEAVNIL 767 >At1g08440.1 68414.m00933 hypothetical protein contains Pfam profile PF01027: Uncharacterized protein family UPF0005 Length = 501 Score = 27.1 bits (57), Expect = 9.6 Identities = 13/44 (29%), Positives = 25/44 (56%) Frame = +1 Query: 37 INKDNLIKMNVIQAVKMYITKMTXESGPGMKVILMDKETTSIVS 168 IN D I M++ + ++ + +M+ ESG MK + + + +I S Sbjct: 289 INSDMQIPMDIKKKIEEPLRRMSSESGKSMKEVSISLKNMTISS 332 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,431,539 Number of Sequences: 28952 Number of extensions: 244469 Number of successful extensions: 684 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 672 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 683 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1197101088 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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