Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30251.Seq
         (605 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g29210.1 68415.m03550 splicing factor PWI domain-containing p...    83   2e-16
At5g47840.1 68418.m05911 adenylate kinase, chloroplast, putative...    28   4.2  
At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    28   4.2  
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    27   7.3  

>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
           protein contains Pfam profile PF01480: PWI domain
          Length = 878

 Score = 82.6 bits (195), Expect = 2e-16
 Identities = 37/77 (48%), Positives = 55/77 (71%)
 Frame = +3

Query: 255 FSDKEKKLMKQMKFGDCLTQQVDMSKVKLDVLKPWITQKITEILNMEDDVVIEYVTNQLE 434
           FS+K+ KLMK  KF   L   VD++KVK+DV+KPWI  ++TE+L +ED+V+I ++   L+
Sbjct: 17  FSNKQAKLMKSQKFAPELENLVDITKVKMDVMKPWIATRVTELLGIEDEVLINFIYGLLD 76

Query: 435 EKFPCPKKMQINLTGFL 485
            K    K++QI LTGF+
Sbjct: 77  GKVVNGKEIQITLTGFM 93



 Score = 27.9 bits (59), Expect = 5.5
 Identities = 17/34 (50%), Positives = 18/34 (52%)
 Frame = +1

Query: 487 MGKXRTPFMGELWELLLSAQAQLKTVYPESFTPA 588
           M K    FM ELW LLLSAQ     V P+ F  A
Sbjct: 93  MEKNTGKFMKELWTLLLSAQNNPSGV-PQQFLDA 125


>At5g47840.1 68418.m05911 adenylate kinase, chloroplast, putative /
           ATP-AMP transphosphorylase, putative similar to
           SP|P43188 Adenylate kinase, chloroplast (EC 2.7.4.3)
           (ATP-AMP transphosphorylase) {Zea mays}; contains Pfam
           profile PF00406: Adenylate kinase
          Length = 283

 Score = 28.3 bits (60), Expect = 4.2
 Identities = 13/45 (28%), Positives = 27/45 (60%)
 Frame = +3

Query: 306 LTQQVDMSKVKLDVLKPWITQKITEILNMEDDVVIEYVTNQLEEK 440
           LTQ+ D ++ K  +      Q ++++L+M DD+ I+   N+ +E+
Sbjct: 214 LTQRFDDTEEKAKLRLKTHNQNVSDVLSMYDDITIKIEGNRSKEE 258


>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein low similarity to SP|Q13144
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Homo sapiens};
           contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 411

 Score = 28.3 bits (60), Expect = 4.2
 Identities = 19/56 (33%), Positives = 33/56 (58%), Gaps = 1/56 (1%)
 Frame = +3

Query: 324 MSKVKLDVLKPWITQKITEILNMEDDVVIEYVTNQLEE-KFPCPKKMQINLTGFLN 488
           M +VKL  +K  +T K+TE  N+  D VIE V  Q+++ K P  + +++   G ++
Sbjct: 248 MFEVKLREIKTVLTSKVTEESNV--DEVIESVKQQIKDAKLPDIEVVRVVWDGLMD 301


>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein low similarity to SP|P47823
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Oryctolagus
           cuniculus}; contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 411

 Score = 27.5 bits (58), Expect = 7.3
 Identities = 17/54 (31%), Positives = 31/54 (57%), Gaps = 1/54 (1%)
 Frame = +3

Query: 330 KVKLDVLKPWITQKITEILNMEDDVVIEYVTNQLEE-KFPCPKKMQINLTGFLN 488
           +VKL  +K  +T ++TE +N+  D V E V  Q+++ K P  + + +   G +N
Sbjct: 250 EVKLKEIKAVLTSQVTEEINV--DEVTEMVKQQVKDAKLPETEVVHVIWDGIMN 301


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,065,447
Number of Sequences: 28952
Number of extensions: 267236
Number of successful extensions: 649
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 634
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 649
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1206913392
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)