BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30221.Seq (764 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value AJ968562-1|CAI91546.1| 998|Apis mellifera protein ( Apis mellif... 25 0.58 DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholi... 22 7.2 DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholi... 22 7.2 AY268031-1|AAP23056.1| 810|Apis mellifera dorsal protein splice... 21 9.5 >AJ968562-1|CAI91546.1| 998|Apis mellifera protein ( Apis mellifera ORF for hypotheticalprotein. ). Length = 998 Score = 25.4 bits (53), Expect = 0.58 Identities = 12/35 (34%), Positives = 18/35 (51%), Gaps = 1/35 (2%) Frame = +2 Query: 605 KSGENLKNRLTIQIDNGYGGVERYAWDHWL-MHAK 706 + E + RL ++D+ GGV HWL +H K Sbjct: 485 EDSEEFRQRLRKELDSKTGGVNLKGHAHWLTLHFK 519 >DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholine receptor alpha4subunit protein. Length = 569 Score = 21.8 bits (44), Expect = 7.2 Identities = 7/23 (30%), Positives = 14/23 (60%) Frame = -2 Query: 700 MHQPMIPSVPLHAAISIINLYGK 632 +H P +P +PLH +++ G+ Sbjct: 436 IHGPPLPPLPLHTECEDLSVSGE 458 >DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholine receptor alpha4subunit protein. Length = 569 Score = 21.8 bits (44), Expect = 7.2 Identities = 7/23 (30%), Positives = 14/23 (60%) Frame = -2 Query: 700 MHQPMIPSVPLHAAISIINLYGK 632 +H P +P +PLH +++ G+ Sbjct: 436 IHGPPLPPLPLHTECEDLSVSGE 458 >AY268031-1|AAP23056.1| 810|Apis mellifera dorsal protein splice variant B protein. Length = 810 Score = 21.4 bits (43), Expect = 9.5 Identities = 8/18 (44%), Positives = 14/18 (77%) Frame = -1 Query: 80 IEKISRDLNIEIHEIPES 27 I+K ++D+N+EI P+S Sbjct: 461 IDKETKDMNLEISTRPKS 478 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 184,567 Number of Sequences: 438 Number of extensions: 3296 Number of successful extensions: 9 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 9 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 9 length of database: 146,343 effective HSP length: 57 effective length of database: 121,377 effective search space used: 23911269 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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