SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30201.Seq
         (671 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g77140.1 68414.m08986 vacuolar protein sorting protein 45, pu...    79   3e-15
At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putat...    37   0.014
At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE...    36   0.032
At2g28070.1 68415.m03408 ABC transporter family protein                29   2.1  
At3g15060.1 68416.m01905 Ras-related GTP-binding family protein ...    29   3.7  
At5g23490.1 68418.m02756 expressed protein                             27   8.6  
At1g71770.1 68414.m08295 polyadenylate-binding protein 5 (PABP5)...    27   8.6  

>At1g77140.1 68414.m08986 vacuolar protein sorting protein 45,
           putative / VPS45p, putative identical to vacuolar
           protein sorting homolog VPS45p [Arabidopsis thaliana]
           gi|2921406|gb|AAC39472
          Length = 569

 Score = 79.0 bits (186), Expect = 3e-15
 Identities = 33/77 (42%), Positives = 54/77 (70%)
 Frame = +3

Query: 255 HMKCIVFIRPTSENIALLSRELRDPKYGVYFIYFSNVVSKADIKTLAECDEXEAVREVQE 434
           H+K + FIRPTS+NI  L  +L +P++G Y ++FSN++    I  LA+ DE E V++VQE
Sbjct: 66  HLKAVYFIRPTSDNIQKLRYQLANPRFGEYHLFFSNLLKDTQIHILADSDEQEVVQQVQE 125

Query: 435 VFADYLAVDRHLFSFNI 485
            +AD+++ D + F+ N+
Sbjct: 126 YYADFVSGDPYHFTLNM 142



 Score = 59.7 bits (138), Expect = 2e-09
 Identities = 31/57 (54%), Positives = 41/57 (71%)
 Frame = +1

Query: 61  MNVIQAVKMYITXMXXXSGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDS 231
           M ++ +V+ YI  M      GMKV+++D ET S VS+VYSQSE+LQKEV+L E IDS
Sbjct: 1   MVLVTSVRDYINRMLQDIS-GMKVLILDSETVSNVSIVYSQSELLQKEVFLVEMIDS 56


>At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putative
           similar to cytokinesis-related Sec1 protein KEULE
           [Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
           Pfam domain, PF00995: Sec1 family
          Length = 662

 Score = 36.7 bits (81), Expect = 0.014
 Identities = 21/84 (25%), Positives = 45/84 (53%), Gaps = 3/84 (3%)
 Frame = +3

Query: 258 MKCIVFIRPTSENIALLSREL--RDPKYGVYFIYFSNVVSKADIKTL-AECDEXEAVREV 428
           M+ I FI+PT EN+     ++  + P Y   F++FS+ VS++ +  +  +    + +  +
Sbjct: 86  MEVIYFIQPTEENVTAFLSDMTGKSPLYKKAFVFFSSPVSRSLVNLIKKDMRAMKRIGGL 145

Query: 429 QEVFADYLAVDRHLFSFNIVGCLQ 500
           +E+  +Y+++D   F  N    L+
Sbjct: 146 KEMNLEYISMDIQGFVTNNENALE 169



 Score = 31.9 bits (69), Expect = 0.40
 Identities = 16/36 (44%), Positives = 21/36 (58%)
 Frame = +1

Query: 127 KVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSH 234
           KV++MDK T  I+S     SEI Q+ + L E I  H
Sbjct: 44  KVLVMDKFTVKIMSSACKMSEITQEGISLVEVITKH 79


>At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE)
           similar to cytokinesis-related Sec1 protein KEULE
           [Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
           Pfam domain, PF00995: Sec1 family
          Length = 666

 Score = 35.5 bits (78), Expect = 0.032
 Identities = 20/75 (26%), Positives = 39/75 (52%), Gaps = 3/75 (4%)
 Frame = +3

Query: 258 MKCIVFIRPTSENIALLSREL--RDPKYGVYFIYFSNVVSKADIKTL-AECDEXEAVREV 428
           M  I FI+PT EN+ +   ++  + P Y   F++FS+ VSK  +  +  +      +  +
Sbjct: 86  MDAIYFIQPTKENVIMFLSDMSGKSPLYKKAFVFFSSPVSKELVGHIKKDSSVLPRIGAL 145

Query: 429 QEVFADYLAVDRHLF 473
           +E+  ++ A+D   F
Sbjct: 146 REMNLEFFAIDSQGF 160


>At2g28070.1 68415.m03408 ABC transporter family protein
          Length = 730

 Score = 29.5 bits (63), Expect = 2.1
 Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 2/46 (4%)
 Frame = -2

Query: 622 SXRXLMLXRSERQARRLQSTG--GAPXRP*ETRWKMLLVPTRPWRH 491
           S   +++  +ER+  +L+S G  GA  R     W+ LLV +R W++
Sbjct: 415 SVEAMIIKLTEREGTQLKSKGKAGAATRVAVLTWRSLLVMSREWKY 460


>At3g15060.1 68416.m01905 Ras-related GTP-binding family protein
           similar to GTP-binding protein GI:303742 from [Pisum
           sativum]; contains Pfam profile: PF00071 ras family
          Length = 217

 Score = 28.7 bits (61), Expect = 3.7
 Identities = 21/56 (37%), Positives = 30/56 (53%), Gaps = 3/56 (5%)
 Frame = -1

Query: 500 LEASXNIEREQVSVHSQ---VVSKNFLDLSDSLXFITFGQSFDVCLRNYITEIYKV 342
           LEA  N+E     V SQ   V SK  LD+ D    +  GQS +V  ++ ++E+ KV
Sbjct: 158 LEA-LNVENAFTEVLSQIYRVASKKALDIGDDHTTLPKGQSINVGSKDDVSEVKKV 212


>At5g23490.1 68418.m02756 expressed protein
          Length = 729

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 20/59 (33%), Positives = 29/59 (49%)
 Frame = -1

Query: 581 SPLAEYRRSAXQTLRNSLEDAVGSNPTLEASXNIEREQVSVHSQVVSKNFLDLSDSLXF 405
           SPLA   RSA   L +S +D++ S+       N+ER+Q         +N+  L DS  F
Sbjct: 359 SPLAP-SRSAGVALTHSTKDSMYSHDHTAIDWNLERQQQDEPGSSAVRNY-HLDDSSTF 415


>At1g71770.1 68414.m08295 polyadenylate-binding protein 5 (PABP5)
           identical to GB:Q05196 from [Arabidopsis thaliana]
          Length = 668

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 17/63 (26%), Positives = 24/63 (38%)
 Frame = +2

Query: 482 YCXMPPRSGWNQQHLPTSFSRSAGRSSCTLQAASLAFATXQHQXPGAPFXGXXGPRFCLK 661
           Y  +  R    Q HL + F++   RS  T+       +   H  PG P  G   P F   
Sbjct: 399 YVALAQRKEERQAHLQSLFTQI--RSPGTMSPVPSPMSGFHHHPPGGPMSGPHHPMFIGH 456

Query: 662 EGK 670
            G+
Sbjct: 457 NGQ 459


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,453,799
Number of Sequences: 28952
Number of extensions: 256062
Number of successful extensions: 594
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 584
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 593
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1422784080
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -