Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30200.Seq
(809 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g36020.1 68417.m05128 cold-shock DNA-binding family protein c... 42 4e-04
At4g38680.1 68417.m05477 cold-shock DNA-binding family protein c... 42 6e-04
At2g17870.1 68415.m02070 cold-shock DNA-binding family protein c... 39 0.003
At2g21060.1 68415.m02500 cold-shock DNA-binding family protein /... 39 0.005
At2g33620.3 68415.m04122 DNA-binding family protein / AT-hook pr... 30 1.6
At2g33620.2 68415.m04121 DNA-binding family protein / AT-hook pr... 30 1.6
At2g33620.1 68415.m04120 DNA-binding family protein / AT-hook pr... 30 1.6
At3g45830.1 68416.m04960 expressed protein 29 2.8
At3g28958.1 68416.m03619 plastocyanin-like domain-containing pro... 29 2.8
At1g25540.1 68414.m03171 phytochrome and flowering time regulato... 28 6.4
>At4g36020.1 68417.m05128 cold-shock DNA-binding family protein
contains Pfam domains, PF00313: 'Cold-shock' DNA-binding
domain and PF00098: Zinc knuckle
Length = 299
Score = 42.3 bits (95), Expect = 4e-04
Identities = 25/62 (40%), Positives = 35/62 (56%), Gaps = 1/62 (1%)
Frame = +1
Query: 241 FHQQDDTKEDVFVHQTAIARNNPRKAVRSVGDGEAVEFAVVAGEKG-FEAAGVTGPGGEP 417
F DD ++FVHQ++I + RS+ G+AVEFA+ G G +A VT PGG
Sbjct: 26 FITPDDGSVELFVHQSSIVS----EGYRSLTVGDAVEFAITQGSDGKTKAVNVTAPGGGS 81
Query: 418 VK 423
+K
Sbjct: 82 LK 83
Score = 30.3 bits (65), Expect = 1.6
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = +2
Query: 188 AEKVSGTVKWFNVKSGYGFI 247
A + +G V WFN GYGFI
Sbjct: 8 AARSTGKVNWFNASKGYGFI 27
>At4g38680.1 68417.m05477 cold-shock DNA-binding family protein
contains Pfam domains PF00313: 'Cold-shock' DNA-binding
domain and PF00098: Zinc knuckle
Length = 203
Score = 41.5 bits (93), Expect = 6e-04
Identities = 27/77 (35%), Positives = 40/77 (51%), Gaps = 1/77 (1%)
Frame = +1
Query: 202 GHCEMVQRQEWIWFHQQDDTKEDVFVHQTAIARNNPRKAVRSVGDGEAVEFAV-VAGEKG 378
G + Q+ F DD +D+FVHQ++I + RS+ EAVEF V +
Sbjct: 13 GSVKWFDTQKGFGFITPDDGGDDLFVHQSSIR----SEGFRSLAAEEAVEFEVEIDNNNR 68
Query: 379 FEAAGVTGPGGEPVKGS 429
+A V+GP G PV+G+
Sbjct: 69 PKAIDVSGPDGAPVQGN 85
Score = 29.9 bits (64), Expect = 2.1
Identities = 10/19 (52%), Positives = 15/19 (78%)
Frame = +2
Query: 191 EKVSGTVKWFNVKSGYGFI 247
E+ G+VKWF+ + G+GFI
Sbjct: 9 ERRKGSVKWFDTQKGFGFI 27
>At2g17870.1 68415.m02070 cold-shock DNA-binding family protein
contains Pfam domains, PF00313: 'Cold-shock' DNA-binding
domain and PF00098: Zinc knuckle
Length = 301
Score = 39.1 bits (87), Expect = 0.003
Identities = 26/73 (35%), Positives = 37/73 (50%), Gaps = 1/73 (1%)
Frame = +1
Query: 196 SIGHCEMVQRQEWIWFHQQDDTKEDVFVHQTAIARNNPRKAVRSVGDGEAVEFAVVAGEK 375
SIG + F DD E++FVHQ++I + RS+ GE+VE+ + G
Sbjct: 11 SIGKVSWFSDGKGYGFITPDDGGEELFVHQSSIVSD----GFRSLTLGESVEYEIALGSD 66
Query: 376 G-FEAAGVTGPGG 411
G +A VT PGG
Sbjct: 67 GKTKAIEVTAPGG 79
>At2g21060.1 68415.m02500 cold-shock DNA-binding family protein /
glycine-rich protein (GRP2) identical to Glycine-rich
protein 2b (AtGRP2b) [Arabidopsis thaliana]
SWISS-PROT:Q38896; contains Pfam domains PF00313:
'Cold-shock' DNA-binding domain and PF00098: Zinc
knuckle
Length = 201
Score = 38.7 bits (86), Expect = 0.005
Identities = 26/77 (33%), Positives = 39/77 (50%), Gaps = 1/77 (1%)
Frame = +1
Query: 202 GHCEMVQRQEWIWFHQQDDTKEDVFVHQTAIARNNPRKAVRSVGDGEAVEFAVVAGEKGF 381
G + Q+ F D +D+FVHQ++I + RS+ E+VEF V G
Sbjct: 17 GTVKWFDTQKGFGFITPSDGGDDLFVHQSSIR----SEGFRSLAAEESVEFDVEVDNSGR 72
Query: 382 -EAAGVTGPGGEPVKGS 429
+A V+GP G PV+G+
Sbjct: 73 PKAIEVSGPDGAPVQGN 89
Score = 30.3 bits (65), Expect = 1.6
Identities = 10/19 (52%), Positives = 15/19 (78%)
Frame = +2
Query: 191 EKVSGTVKWFNVKSGYGFI 247
++ GTVKWF+ + G+GFI
Sbjct: 13 DRRKGTVKWFDTQKGFGFI 31
>At2g33620.3 68415.m04122 DNA-binding family protein / AT-hook
protein 1 (AHP1) identical to AT-hook protein 1
[Arabidopsis thaliana] gi|2598227|emb|CAA10857
Length = 351
Score = 30.3 bits (65), Expect = 1.6
Identities = 20/51 (39%), Positives = 25/51 (49%)
Frame = +1
Query: 325 SVGDGEAVEFAVVAGEKGFEAAGVTGPGGEPVKGSPYAADKRRGYHRQYFP 477
S G+ + + GE G G+TG G EPVK KRRG R+Y P
Sbjct: 69 SAGENSVLNMNLPGGESG----GMTGTGSEPVK-------KRRGRPRKYGP 108
>At2g33620.2 68415.m04121 DNA-binding family protein / AT-hook
protein 1 (AHP1) identical to AT-hook protein 1
[Arabidopsis thaliana] gi|2598227|emb|CAA10857
Length = 351
Score = 30.3 bits (65), Expect = 1.6
Identities = 20/51 (39%), Positives = 25/51 (49%)
Frame = +1
Query: 325 SVGDGEAVEFAVVAGEKGFEAAGVTGPGGEPVKGSPYAADKRRGYHRQYFP 477
S G+ + + GE G G+TG G EPVK KRRG R+Y P
Sbjct: 69 SAGENSVLNMNLPGGESG----GMTGTGSEPVK-------KRRGRPRKYGP 108
>At2g33620.1 68415.m04120 DNA-binding family protein / AT-hook
protein 1 (AHP1) identical to AT-hook protein 1
[Arabidopsis thaliana] gi|2598227|emb|CAA10857
Length = 351
Score = 30.3 bits (65), Expect = 1.6
Identities = 20/51 (39%), Positives = 25/51 (49%)
Frame = +1
Query: 325 SVGDGEAVEFAVVAGEKGFEAAGVTGPGGEPVKGSPYAADKRRGYHRQYFP 477
S G+ + + GE G G+TG G EPVK KRRG R+Y P
Sbjct: 69 SAGENSVLNMNLPGGESG----GMTGTGSEPVK-------KRRGRPRKYGP 108
>At3g45830.1 68416.m04960 expressed protein
Length = 1298
Score = 29.5 bits (63), Expect = 2.8
Identities = 21/81 (25%), Positives = 35/81 (43%), Gaps = 2/81 (2%)
Frame = +1
Query: 220 QRQEWIWFHQQDDTK--EDVFVHQTAIARNNPRKAVRSVGDGEAVEFAVVAGEKGFEAAG 393
+R+ W++ H+ + + ED T + ++A + EAV A + E+ E
Sbjct: 1161 ERKLWVYLHRDREQEDFEDDGTSSTKKWKRPKKEAAEQTEEQEAVTVAFLGNEEQTE--- 1217
Query: 394 VTGPGGEPVKGSPYAADKRRG 456
T G EP G P D +G
Sbjct: 1218 -TEMGSEPKTGEPTGLDGDQG 1237
>At3g28958.1 68416.m03619 plastocyanin-like domain-containing
protein contains plastocyanin-like domain Pfam:PF02298
Length = 175
Score = 29.5 bits (63), Expect = 2.8
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = -3
Query: 714 GWKIARNAIRRNLPLFNWARTSPTAVHRENXD 619
GW +++ I R+L + TSP A+H+ D
Sbjct: 95 GWSVSQEKISRDLEFISCDPTSPVAMHKTGHD 126
>At1g25540.1 68414.m03171 phytochrome and flowering time regulatory
protein (PFT1) PMID: 12815435
Length = 836
Score = 28.3 bits (60), Expect = 6.4
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = +2
Query: 218 FNVKSGYGFINRMTPRKMCLCIRLPS 295
F S +GF+ ++ +K+C I+LPS
Sbjct: 623 FRAMSQHGFLGQLQDKKLCAVIQLPS 648
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,927,978
Number of Sequences: 28952
Number of extensions: 260913
Number of successful extensions: 734
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 708
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 732
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1843581600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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