Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30186.Seq
(767 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 178 4e-47
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 178 4e-47
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 175 5e-46
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 175 5e-46
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 36 5e-04
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.4
DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 3.1
AY338499-1|AAR08420.1| 500|Apis mellifera Kruppel-like protein ... 23 3.1
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.1
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 4.1
DQ342041-1|ABC69933.1| 828|Apis mellifera STIP protein. 22 7.2
AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 21 9.5
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 178 bits (434), Expect = 4e-47
Identities = 85/85 (100%), Positives = 85/85 (100%)
Frame = +3
Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434
TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG
Sbjct: 15 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 74
Query: 435 QTREHALLAFTLGVKQLIVGVNKMD 509
QTREHALLAFTLGVKQLIVGVNKMD
Sbjct: 75 QTREHALLAFTLGVKQLIVGVNKMD 99
Score = 118 bits (284), Expect = 6e-29
Identities = 53/67 (79%), Positives = 57/67 (85%)
Frame = +2
Query: 509 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDXMLEPSTKMXWFXGWQW 688
STEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGD MLE S+KM WF GW
Sbjct: 100 STEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTV 159
Query: 689 SVRKAKL 709
++ K+
Sbjct: 160 ERKEGKV 166
Score = 40.3 bits (90), Expect = 2e-05
Identities = 17/26 (65%), Positives = 20/26 (76%)
Frame = +1
Query: 685 VERKEGKADXKCSLKLLDAILPTARP 762
VERKEGK + KC ++ LDAILP RP
Sbjct: 159 VERKEGKVEGKCLIEALDAILPPTRP 184
Score = 27.5 bits (58), Expect = 0.15
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +1
Query: 214 WVLDKLKAERE 246
WVLDKLKAERE
Sbjct: 1 WVLDKLKAERE 11
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 178 bits (434), Expect = 4e-47
Identities = 85/85 (100%), Positives = 85/85 (100%)
Frame = +3
Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434
TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG
Sbjct: 72 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 131
Query: 435 QTREHALLAFTLGVKQLIVGVNKMD 509
QTREHALLAFTLGVKQLIVGVNKMD
Sbjct: 132 QTREHALLAFTLGVKQLIVGVNKMD 156
Score = 145 bits (352), Expect = 3e-37
Identities = 67/68 (98%), Positives = 67/68 (98%)
Frame = +1
Query: 43 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 222
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 223 DKLKAERE 246
DKLKAERE
Sbjct: 61 DKLKAERE 68
Score = 118 bits (284), Expect = 6e-29
Identities = 53/67 (79%), Positives = 57/67 (85%)
Frame = +2
Query: 509 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDXMLEPSTKMXWFXGWQW 688
STEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGD MLE S+KM WF GW
Sbjct: 157 STEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTV 216
Query: 689 SVRKAKL 709
++ K+
Sbjct: 217 ERKEGKV 223
Score = 40.3 bits (90), Expect = 2e-05
Identities = 17/26 (65%), Positives = 20/26 (76%)
Frame = +1
Query: 685 VERKEGKADXKCSLKLLDAILPTARP 762
VERKEGK + KC ++ LDAILP RP
Sbjct: 216 VERKEGKVEGKCLIEALDAILPPTRP 241
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 175 bits (425), Expect = 5e-46
Identities = 83/85 (97%), Positives = 84/85 (98%)
Frame = +3
Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434
TIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG GEFEAGISKNG
Sbjct: 72 TIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISKNG 131
Query: 435 QTREHALLAFTLGVKQLIVGVNKMD 509
QTREHALLAFTLGVKQLIVGVNKMD
Sbjct: 132 QTREHALLAFTLGVKQLIVGVNKMD 156
Score = 145 bits (352), Expect = 3e-37
Identities = 67/68 (98%), Positives = 67/68 (98%)
Frame = +1
Query: 43 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 222
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 223 DKLKAERE 246
DKLKAERE
Sbjct: 61 DKLKAERE 68
Score = 109 bits (263), Expect = 2e-26
Identities = 47/58 (81%), Positives = 51/58 (87%)
Frame = +2
Query: 512 TEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDXMLEPSTKMXWFXGWQ 685
T+PPYSE RFEEIKKEVSSYIKKIGYN A+VAFVPISGWHGD MLEPS K W+ GW+
Sbjct: 158 TDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWK 215
Score = 36.7 bits (81), Expect = 2e-04
Identities = 16/26 (61%), Positives = 20/26 (76%)
Frame = +1
Query: 685 VERKEGKADXKCSLKLLDAILPTARP 762
VERK+G AD K ++ LDAILP +RP
Sbjct: 216 VERKDGNADGKTLIEALDAILPPSRP 241
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 175 bits (425), Expect = 5e-46
Identities = 83/83 (100%), Positives = 83/83 (100%)
Frame = +3
Query: 261 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 440
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 441 REHALLAFTLGVKQLIVGVNKMD 509
REHALLAFTLGVKQLIVGVNKMD
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMD 83
Score = 118 bits (284), Expect = 6e-29
Identities = 53/67 (79%), Positives = 57/67 (85%)
Frame = +2
Query: 509 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDXMLEPSTKMXWFXGWQW 688
STEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGD MLE S+KM WF GW
Sbjct: 84 STEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTV 143
Query: 689 SVRKAKL 709
++ K+
Sbjct: 144 ERKEGKV 150
Score = 40.3 bits (90), Expect = 2e-05
Identities = 17/26 (65%), Positives = 20/26 (76%)
Frame = +1
Query: 685 VERKEGKADXKCSLKLLDAILPTARP 762
VERKEGK + KC ++ LDAILP RP
Sbjct: 143 VERKEGKVEGKCLIEALDAILPPTRP 168
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 35.5 bits (78), Expect = 5e-04
Identities = 27/73 (36%), Positives = 34/73 (46%)
Frame = +3
Query: 300 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 479
VT +D PGH FI G D VL+VAA G E QT + +A V
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246
Query: 480 QLIVGVNKMDPLN 518
+IV +NK+D N
Sbjct: 247 PIIVAINKIDKPN 259
Score = 25.4 bits (53), Expect = 0.59
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 55 KTHINIVVIGHVDSGKST 108
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.4
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1
Query: 64 INIVVIGHVDSGKST 108
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 517
Score = 23.0 bits (47), Expect = 3.1
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -1
Query: 56 FSLPIFG*SRITNCV*Y 6
FSLPIFG I +C+ Y
Sbjct: 57 FSLPIFGTRWIFSCIGY 73
>AY338499-1|AAR08420.1| 500|Apis mellifera Kruppel-like protein 1
protein.
Length = 500
Score = 23.0 bits (47), Expect = 3.1
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = +3
Query: 465 TLGVKQLIVGVNKMDPLNHHTVSPDL 542
T+ VK I V +DP+ SPDL
Sbjct: 15 TMSVKNEISTVEPVDPVKSLVCSPDL 40
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 3.1
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +3
Query: 285 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 386
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 4.1
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +3
Query: 285 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 386
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>DQ342041-1|ABC69933.1| 828|Apis mellifera STIP protein.
Length = 828
Score = 21.8 bits (44), Expect = 7.2
Identities = 9/28 (32%), Positives = 16/28 (57%)
Frame = +2
Query: 479 TAHRRSKQNGSTEPPYSEPRFEEIKKEV 562
T+ R++ S PP ++PR E + + V
Sbjct: 725 TSLERTQPTMSQMPPTAQPRMERLAEAV 752
>AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic
acetylcholine Apisa7-2 subunit protein.
Length = 461
Score = 21.4 bits (43), Expect = 9.5
Identities = 8/24 (33%), Positives = 13/24 (54%)
Frame = -2
Query: 661 FG*RLQHVVSVPSRNGHESDSSWV 590
FG L H++ V +N + + WV
Sbjct: 35 FGLSLHHIIDVDEKNQILTTNCWV 58
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 214,807
Number of Sequences: 438
Number of extensions: 4283
Number of successful extensions: 32
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 24032646
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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