BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30182.Seq (827 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC22A12.08c |||cardiolipin synthase/ hydrolase fusion protein ... 60 5e-10 SPCC1442.12 |||CDP-diacylglycerol--serine O-phosphatidyltransfer... 35 0.012 SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ... 31 0.15 SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltra... 31 0.26 SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyc... 27 4.3 SPAC20G8.03 |itr2||MFS myo-inositol transporter|Schizosaccharomy... 26 7.5 SPAC12B10.11 |exg2||glucan 1,3-beta-glucosidase Exg2|Schizosacch... 26 7.5 SPAC6F6.02c |pof5||F-box protein Pof5|Schizosaccharomyces pombe|... 26 7.5 >SPAC22A12.08c |||cardiolipin synthase/ hydrolase fusion protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 570 Score = 59.7 bits (138), Expect = 5e-10 Identities = 28/58 (48%), Positives = 39/58 (67%) Frame = -2 Query: 505 QDLIPISLTLLIVGRDIALVVAGFVIRYISLPPPRTLSRYFDVTHATAQLAPTFISKV 332 ++ +P++L LI+GRD+ LV A +RY SLP P+T R+FD T +L PT ISKV Sbjct: 478 RETMPLTLASLIIGRDVLLVSAVSYLRYKSLPAPKTFRRFFDFAIPTTELKPTRISKV 535 Score = 43.2 bits (97), Expect = 5e-05 Identities = 23/47 (48%), Positives = 31/47 (65%) Frame = -3 Query: 642 VFAGITDLLDGWIARNWKGQSTKMGSFLDPMADKVLVATLFISLTGR 502 ++AG TDL+DG+IAR + S G+ LDP+ADK L+ L I L R Sbjct: 433 LYAGFTDLVDGYIARKFDLGSI-AGTVLDPLADKTLMTCLTICLAVR 478 >SPCC1442.12 |||CDP-diacylglycerol--serine O-phosphatidyltransferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 250 Score = 35.1 bits (77), Expect = 0.012 Identities = 17/34 (50%), Positives = 23/34 (67%) Frame = -3 Query: 639 FAGITDLLDGWIARNWKGQSTKMGSFLDPMADKV 538 FA D LDG +AR W+G+S+ MG LD +AD + Sbjct: 87 FALFFDFLDGKVAR-WRGKSSLMGQELDSLADLI 119 >SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1877 Score = 31.5 bits (68), Expect = 0.15 Identities = 19/62 (30%), Positives = 27/62 (43%), Gaps = 2/62 (3%) Frame = -3 Query: 669 ITTWHWGLLVFAGITDLLDGWIARNWKGQ--STKMGSFLDPMADKVLVATLFISLTGRTS 496 IT W+W + + L I RNWK + + K G F + L + S T R S Sbjct: 1065 ITLWNWMMNQVPSFSSFLISNIIRNWKNEIVTEKRGYFSTAKSKSPLALPMTYSPTERAS 1124 Query: 495 YL 490 +L Sbjct: 1125 FL 1126 >SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltransferase Pis1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 251 Score = 30.7 bits (66), Expect = 0.26 Identities = 14/44 (31%), Positives = 23/44 (52%) Frame = -3 Query: 642 VFAGITDLLDGWIARNWKGQSTKMGSFLDPMADKVLVATLFISL 511 +++ + D DGW AR Q+T G+ LD + D+ + L L Sbjct: 60 LYSSLLDAFDGWAARKLH-QATNFGAILDMVTDRCATSCLLCFL 102 >SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1428 Score = 26.6 bits (56), Expect = 4.3 Identities = 14/42 (33%), Positives = 22/42 (52%) Frame = -1 Query: 326 SCSTSIGRHYAGVPSFWLC*SSSIKVLCGITAASTIVSAVSY 201 S SI R +A +P S + ++LCG+ AAS + + Y Sbjct: 1240 SLKRSICRRFAVIPKEHDINSGNAEILCGVIAASLYPNILRY 1281 >SPAC20G8.03 |itr2||MFS myo-inositol transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 557 Score = 25.8 bits (54), Expect = 7.5 Identities = 21/63 (33%), Positives = 30/63 (47%), Gaps = 5/63 (7%) Frame = -3 Query: 669 ITTWHWGLLVFAGITDLLDGWIARNWKGQSTKMGSFLDPM---ADKVLV--ATLFISLTG 505 I++W W L AGI+ LL G+ G +GS L + K L+ AT F +L Sbjct: 77 ISSWIWVLSAVAGISGLLFGYDTGVISGALAVLGSDLGHVLSSGQKELITSATSFAALIS 136 Query: 504 RTS 496 T+ Sbjct: 137 ATT 139 >SPAC12B10.11 |exg2||glucan 1,3-beta-glucosidase Exg2|Schizosaccharomyces pombe|chr 1|||Manual Length = 570 Score = 25.8 bits (54), Expect = 7.5 Identities = 11/22 (50%), Positives = 14/22 (63%) Frame = +2 Query: 719 SDT*SGLG*WKDIFFPYNFPQI 784 SD G G W+D F P++FP I Sbjct: 372 SDGFRGPGNWEDHFDPFHFPNI 393 >SPAC6F6.02c |pof5||F-box protein Pof5|Schizosaccharomyces pombe|chr 1|||Manual Length = 348 Score = 25.8 bits (54), Expect = 7.5 Identities = 16/51 (31%), Positives = 26/51 (50%) Frame = +3 Query: 327 VFTLLIKVGASCAVACVTSKYLLNVRGGGKLIYRMTNPATTNAISRPTISS 479 +F+LL K+G + + CV R G I+++ ATT +I I+S Sbjct: 213 IFSLLSKIGPNLSSVCVQYPMPELSRSGLDCIFQLCPNATTISIPANYITS 263 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,303,441 Number of Sequences: 5004 Number of extensions: 68848 Number of successful extensions: 148 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 141 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 147 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 406444570 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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