Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30179.Seq
         (844 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac...    96   6e-21
SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pomb...    31   0.15 
SPAC1556.02c |sdh1||succinate dehydrogenase Sdh1|Schizosaccharom...    30   0.36 
SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein S15a...    29   1.1  
SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein S...    29   1.1  
SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schi...    28   1.4  
SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac...    28   1.9  
SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr...    27   3.3  
SPBP4G3.03 |||PI31 proteasome regulator related|Schizosaccharomy...    26   5.8  
SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   7.7  
SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr ...    26   7.7  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   7.7  

>SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase
           Cdc48|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 815

 Score = 95.9 bits (228), Expect = 6e-21
 Identities = 43/100 (43%), Positives = 63/100 (63%)
 Frame = +1

Query: 223 LAQGQTPQGNRCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 402
           + +G+  +    IVL+D+   D   R+             D+V+I PCP +KY +R+ +L
Sbjct: 78  VVKGKRRKDTVLIVLTDEEMEDGVARINRVVRNNLRVRLGDIVTINPCPDIKYAERISVL 137

Query: 403 PIDDSVEGLTGNLFEVYLKPYFMEAYRPIHRDDTFRSAGA 522
           P+ D+VEGLTG+LF+VYLKPYF+EAYRPI + D F   G+
Sbjct: 138 PLADTVEGLTGSLFDVYLKPYFVEAYRPIRKGDLFVVRGS 177



 Score = 88.6 bits (210), Expect = 1e-18
 Identities = 41/68 (60%), Positives = 53/68 (77%)
 Frame = +2

Query: 53  NKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLK 232
           N   D  S +D +TAILR+K +PN L+V++A +DDNSV+ LS   ME LQLFRGDTV++K
Sbjct: 21  NPPKDTYSAEDTATAILRKKRKPNSLVVDDATNDDNSVITLSSNTMETLQLFRGDTVVVK 80

Query: 233 GKRRKETV 256
           GKRRK+TV
Sbjct: 81  GKRRKDTV 88



 Score = 70.5 bits (165), Expect = 3e-13
 Identities = 34/57 (59%), Positives = 40/57 (70%)
 Frame = +3

Query: 510 VRGGMRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIKREEEEGSTKMLSGYXDIXGC 680
           VRG MR VEFKVV+  P  F IV+ DT+IH +GEPI RE+EE S   + GY DI GC
Sbjct: 174 VRGSMRQVEFKVVDVAPDEFGIVSQDTIIHWEGEPINREDEESSLAEV-GYDDIGGC 229


>SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1379

 Score = 31.5 bits (68), Expect = 0.15
 Identities = 18/55 (32%), Positives = 25/55 (45%)
 Frame = -2

Query: 759  SPXXXXRMXASAKLHXLLKFGASCXADNRXCXHSPTAF*CXPLLPHV*SVHRHSV 595
            +P       +S+K H      A+  A+     H+PT     P+LP V   HRHSV
Sbjct: 975  TPSIEETSYSSSKAHDASLSAATLVANKDKLIHAPTPQVLAPVLPKVSLPHRHSV 1029


>SPAC1556.02c |sdh1||succinate dehydrogenase
           Sdh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 641

 Score = 30.3 bits (65), Expect = 0.36
 Identities = 19/56 (33%), Positives = 28/56 (50%), Gaps = 1/56 (1%)
 Frame = -3

Query: 548 DHFELDGAHAPAD-LKVSSRWIGR*ASMKYGFKYTSNRLPVRPSTESSIGSMWTRF 384
           DH  L  +H PA+ LK     I   A++  G   T   +PV P+   ++G + TRF
Sbjct: 355 DHCYLQLSHLPAEILKERLPGISETAAIFAGVDVTKEPIPVLPTVHYNMGGIPTRF 410


>SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein
           S15a|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 130

 Score = 28.7 bits (61), Expect = 1.1
 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%)
 Frame = -1

Query: 421 QLNHQLAVCGLV---FHISLKDTERWI 350
           QLN ++  CG++   F++ LKD E+W+
Sbjct: 64  QLNGRINKCGVISPRFNVKLKDIEKWV 90


>SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein
           S15a|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 130

 Score = 28.7 bits (61), Expect = 1.1
 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%)
 Frame = -1

Query: 421 QLNHQLAVCGLV---FHISLKDTERWI 350
           QLN ++  CG++   F++ LKD E+W+
Sbjct: 64  QLNGRINKCGVISPRFNVKLKDIEKWV 90


>SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 319

 Score = 28.3 bits (60), Expect = 1.4
 Identities = 12/39 (30%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
 Frame = +3

Query: 78  LMIYRPRSSVARTDPTVSLSKKQSAMTTQSWH-FHRPKW 191
           L + R    +   DP ++ +  Q  + T+SWH FH  +W
Sbjct: 259 LALIRGCDGLLSDDPVMARALSQGPIVTKSWHYFHYSEW 297


>SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase
           E3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 905

 Score = 27.9 bits (59), Expect = 1.9
 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
 Frame = -1

Query: 391 LVFHISLKDTERWIP-HQKDAHEGCFSQHGSSEFSHQG 281
           LV  + LKD   WI    + + E  F   G ++FS+QG
Sbjct: 435 LVSDLYLKDLWSWIHLSHRQSEESLFGDTGDTDFSYQG 472


>SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 382

 Score = 27.1 bits (57), Expect = 3.3
 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 2/62 (3%)
 Frame = +3

Query: 27  FLDSNNKF*IKWQIIKALMIYRPRSSVARTDPTVS--LSKKQSAMTTQSWHFHRPKWSNF 200
           F+++N K   KW++  A+    P  S + + PT+S   + + SA TT     +RP  S  
Sbjct: 286 FIEANIK---KWRLAPAVGSLAPPPSYSTSQPTISWDAASESSAGTTTQGDTNRPDKSKN 342

Query: 201 NS 206
            S
Sbjct: 343 RS 344


>SPBP4G3.03 |||PI31 proteasome regulator related|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 241

 Score = 26.2 bits (55), Expect = 5.8
 Identities = 14/48 (29%), Positives = 21/48 (43%)
 Frame = -1

Query: 391 LVFHISLKDTERWIPHQKDAHEGCFSQHGSSEFSHQGNYRLRARCNGF 248
           L FH ++ D   W PH+ +   G F  H     + +G +  R    GF
Sbjct: 177 LGFHPNVGDYFDWYPHRPNHFGGKFDGHSRGRGAGRGGFCGRHGRGGF 224


>SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 452

 Score = 25.8 bits (54), Expect = 7.7
 Identities = 14/47 (29%), Positives = 25/47 (53%)
 Frame = +3

Query: 99  SSVARTDPTVSLSKKQSAMTTQSWHFHRPKWSNFNSSVVTQSCSRAN 239
           SSV       S S   ++M++ + +F+    S+ +SSV T + S +N
Sbjct: 329 SSVVIVQSETSCSINSASMSSNTTYFYWNSTSSLSSSVFTNTTSSSN 375


>SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 409

 Score = 25.8 bits (54), Expect = 7.7
 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 1/27 (3%)
 Frame = +2

Query: 77  PD-DLSTAILRRKDRPNRLIVEEAVSD 154
           PD D + + + RK+ PNRL++  A+S+
Sbjct: 233 PDADANISKIARKEDPNRLVLASAISE 259


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 7.7
 Identities = 14/53 (26%), Positives = 30/53 (56%)
 Frame = +2

Query: 50  LNKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLF 208
           L  +A N   + L+T   +RK + N  ++ +A ++ N+  +++ A  E+ QL+
Sbjct: 768 LGSLAGNLPQESLTTKS-KRKSKVNLELMFDATAEKNNENSMNSAVFEKSQLY 819


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,269,277
Number of Sequences: 5004
Number of extensions: 63966
Number of successful extensions: 189
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 188
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 416455520
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)