Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30176.Seq
(834 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g01800.1 68417.m00237 preprotein translocase secA subunit, pu... 30 1.6
At3g02210.1 68416.m00202 phytochelatin synthetase family protein... 29 3.8
At4g12440.1 68417.m01968 adenine phosphoribosyltransferase, puta... 29 5.0
At3g29810.1 68416.m03794 phytochelatin synthetase family protein... 29 5.0
At3g18860.2 68416.m02396 transducin family protein / WD-40 repea... 29 5.0
At3g18860.1 68416.m02395 transducin family protein / WD-40 repea... 29 5.0
At1g77320.1 68414.m09005 BRCT domain-containing protein contains... 29 5.0
At2g41540.2 68415.m05134 NAD-dependent glycerol-3-phosphate dehy... 28 8.8
At2g41540.1 68415.m05133 NAD-dependent glycerol-3-phosphate dehy... 28 8.8
At2g33420.1 68415.m04096 expressed protein 28 8.8
>At4g01800.1 68417.m00237 preprotein translocase secA subunit,
putative similar to preprotein translocase secA subunit,
chloroplast [precursor] SP:Q9SYI0 from [Arabidopsis
thaliana]; non-consensus GA donor splice site at exon 4
Length = 1022
Score = 30.3 bits (65), Expect = 1.6
Identities = 15/50 (30%), Positives = 28/50 (56%)
Frame = +1
Query: 379 DDRRRRVPHYWVPVLKRTVVRSEVLNTHLSVTVTDRTISLINDPMGLTII 528
D+ +R+V +Y+ + K+ EVLN+ T+R +L++D + II
Sbjct: 774 DEAQRKVENYFFDIRKQLFEFDEVLNSQRDRVYTERRRALVSDSLEPLII 823
>At3g02210.1 68416.m00202 phytochelatin synthetase family protein /
COBRA cell expansion protein COBL3 similar to
phytochelatin synthetase [Hordeum vulgare subsp.
vulgare] GI:29570314; identified in Roudier, et al,
Plant Phys. (2002) 130:538-548 (PMID:12376623);
supported by cDNA: gi|26452134|dbj|AK118555.1; contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 452
Score = 29.1 bits (62), Expect = 3.8
Identities = 15/50 (30%), Positives = 29/50 (58%)
Frame = -1
Query: 465 EMSIENFRSYYSSLQYWHPVVRYPASSIITLLETFNHSFSSPYPLIDFSG 316
+++I NF +Y + W+ VV++P +T +FN+ +PY I+ +G
Sbjct: 312 KVTITNF-NYNMNYSQWNLVVQHPNFDNLTQTFSFNYKPLTPYASINDTG 360
>At4g12440.1 68417.m01968 adenine phosphoribosyltransferase,
putative strong similarity to SP|P31166 Adenine
phosphoribosyltransferase 1 (EC 2.4.2.7) (APRT)
{Arabidopsis thaliana}; contains Pfam profile PF00156:
Phosphoribosyl transferase domain
Length = 178
Score = 28.7 bits (61), Expect = 5.0
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = -1
Query: 96 CKREDAIELLYSFFGSINLWGKILTEFQWVTI 1
C + + +SFF +I+L K+L F+W+ I
Sbjct: 139 CAAMNLLSKFFSFFNNISLVPKLLLSFKWLPI 170
>At3g29810.1 68416.m03794 phytochelatin synthetase family protein /
COBRA cell expansion protein COBL2 similar to
phytochelatin synthetase [Hordeum vulgare subsp.
vulgare] GI:29570314; identified in Roudier, et al,
Plant Phys. (2002) 130:538-548 (PMID:12376623); contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 441
Score = 28.7 bits (61), Expect = 5.0
Identities = 14/43 (32%), Positives = 26/43 (60%)
Frame = -1
Query: 465 EMSIENFRSYYSSLQYWHPVVRYPASSIITLLETFNHSFSSPY 337
+++I NF +Y + W+ VV++P +T L +FN+ +PY
Sbjct: 303 KVAITNF-NYNMNYSQWNLVVQHPNFDNLTKLFSFNYKPLNPY 344
>At3g18860.2 68416.m02396 transducin family protein / WD-40 repeat
family protein contains seven G-protein beta WD-40
repeats; similar to phospholipase a-2-activating protein
SP:P27612 from [Mus musculus]
Length = 760
Score = 28.7 bits (61), Expect = 5.0
Identities = 15/69 (21%), Positives = 29/69 (42%)
Frame = -1
Query: 552 NHKQESSVDNGQTHRIINETNSSIRYSY*EMSIENFRSYYSSLQYWHPVVRYPASSIITL 373
N K + ++ + I+N + Y + + LQ W P + +PA+ I+ +
Sbjct: 522 NDKSLTELEVSRVGAIVNILKDTSHYHSTNFADMDIALLLKVLQAWPPAMMFPATDIVRM 581
Query: 372 LETFNHSFS 346
L +H S
Sbjct: 582 LVLHHHGAS 590
>At3g18860.1 68416.m02395 transducin family protein / WD-40 repeat
family protein contains seven G-protein beta WD-40
repeats; similar to phospholipase a-2-activating protein
SP:P27612 from [Mus musculus]
Length = 760
Score = 28.7 bits (61), Expect = 5.0
Identities = 15/69 (21%), Positives = 29/69 (42%)
Frame = -1
Query: 552 NHKQESSVDNGQTHRIINETNSSIRYSY*EMSIENFRSYYSSLQYWHPVVRYPASSIITL 373
N K + ++ + I+N + Y + + LQ W P + +PA+ I+ +
Sbjct: 522 NDKSLTELEVSRVGAIVNILKDTSHYHSTNFADMDIALLLKVLQAWPPAMMFPATDIVRM 581
Query: 372 LETFNHSFS 346
L +H S
Sbjct: 582 LVLHHHGAS 590
>At1g77320.1 68414.m09005 BRCT domain-containing protein contains
Pfam profile PF00533: BRCA1 C Terminus (BRCT) domain
Length = 783
Score = 28.7 bits (61), Expect = 5.0
Identities = 14/52 (26%), Positives = 25/52 (48%)
Frame = +1
Query: 226 TFRSSRREVERDELTGETYPIQNIPIPLKFPAEINEGIWGGEAVVKGFQKRD 381
TF S + ++ RD + +P++ +P P+ +GI G V G + D
Sbjct: 625 TFPSKKAKLLRDGQESDVFPVRELPSNCDRPSHSGDGIVTGYDVASGREVPD 676
>At2g41540.2 68415.m05134 NAD-dependent glycerol-3-phosphate
dehydrogenase family protein weak similarity to
SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
(EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
PF01210: NAD-dependent glycerol-3-phosphate
dehydrogenase
Length = 462
Score = 27.9 bits (59), Expect = 8.8
Identities = 16/65 (24%), Positives = 28/65 (43%), Gaps = 5/65 (7%)
Frame = +1
Query: 232 RSSRREVERDELTGETYPIQNI--PI-PLKFPAEINEGIWGGEAVVKGFQKRDDRR--RR 396
R R + DE+ + + + + P+ PLK + E +W + VV G + R
Sbjct: 118 RLGDRTLYADEILKDGFCLNMVDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEE 177
Query: 397 VPHYW 411
+ YW
Sbjct: 178 ISKYW 182
>At2g41540.1 68415.m05133 NAD-dependent glycerol-3-phosphate
dehydrogenase family protein weak similarity to
SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
(EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
PF01210: NAD-dependent glycerol-3-phosphate
dehydrogenase
Length = 462
Score = 27.9 bits (59), Expect = 8.8
Identities = 16/65 (24%), Positives = 28/65 (43%), Gaps = 5/65 (7%)
Frame = +1
Query: 232 RSSRREVERDELTGETYPIQNI--PI-PLKFPAEINEGIWGGEAVVKGFQKRDDRR--RR 396
R R + DE+ + + + + P+ PLK + E +W + VV G + R
Sbjct: 118 RLGDRTLYADEILKDGFCLNMVDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEE 177
Query: 397 VPHYW 411
+ YW
Sbjct: 178 ISKYW 182
>At2g33420.1 68415.m04096 expressed protein
Length = 1039
Score = 27.9 bits (59), Expect = 8.8
Identities = 13/27 (48%), Positives = 20/27 (74%)
Frame = +2
Query: 275 KLIRYKIFLFLLNSPLKSMRGYGEEKL 355
KL R +++ LLNS L SM+G+ E++L
Sbjct: 405 KLDREALYVKLLNSTLASMQGWTEKRL 431
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,435,119
Number of Sequences: 28952
Number of extensions: 328526
Number of successful extensions: 883
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 867
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 883
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1921616800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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