Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30160.Seq
(904 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_37396| Best HMM Match : PEPCK (HMM E-Value=0) 144 7e-35
SB_3810| Best HMM Match : PEPCK (HMM E-Value=0) 141 6e-34
SB_37397| Best HMM Match : PEPCK (HMM E-Value=0) 135 4e-32
SB_50513| Best HMM Match : Lig_chan (HMM E-Value=0.0083) 29 6.8
SB_15741| Best HMM Match : MANEC (HMM E-Value=2.7) 28 9.0
>SB_37396| Best HMM Match : PEPCK (HMM E-Value=0)
Length = 549
Score = 144 bits (350), Expect = 7e-35
Identities = 76/128 (59%), Positives = 86/128 (67%), Gaps = 5/128 (3%)
Frame = +3
Query: 249 SEVLEILRQDEQFVRCLHAVGSGGTPG-----WPCDPKNTIILHKPAENEIVXXXXXXXX 413
S VL+ L D FVRCLH+VG G WPCDP+ T+I H P EI
Sbjct: 40 SHVLKALG-DGDFVRCLHSVGGPLHDGKNNVPWPCDPERTLITHFPETREIKSYGSGYGG 98
Query: 414 XXXXXKKCFALRLGSVIARREGWLAEHMLIVGITNPQGKKRYIAAAFPSACGKTNLAMMT 593
KKCFALR+ SVIAR EGWLAEHM+I+G+TNP+GKK YIAAAFPSACGKTNLAM+T
Sbjct: 99 NSLLGKKCFALRIASVIARDEGWLAEHMMILGLTNPEGKKIYIAAAFPSACGKTNLAMLT 158
Query: 594 PNTGPGTK 617
P T PG K
Sbjct: 159 P-TIPGWK 165
Score = 70.9 bits (166), Expect = 1e-12
Identities = 30/40 (75%), Positives = 37/40 (92%)
Frame = +1
Query: 133 MYVIPFSMGPVGSPLSKIGVEITDSPYVVYSMRVMTRIGA 252
MYV+PFSMGPVGSP+SKIG+E+TDS YVV MR+MTR+G+
Sbjct: 1 MYVMPFSMGPVGSPISKIGIELTDSEYVVCCMRIMTRMGS 40
Score = 30.3 bits (65), Expect = 2.2
Identities = 17/36 (47%), Positives = 21/36 (58%)
Frame = +2
Query: 605 PGYKVXCVGDDIALMKFRXRTAYFRGHLXRKNGFFG 712
PG+K CVGDDIA M F + R + + GFFG
Sbjct: 162 PGWKCECVGDDIAWMWF-DKDGTLRA-INPECGFFG 195
>SB_3810| Best HMM Match : PEPCK (HMM E-Value=0)
Length = 707
Score = 141 bits (342), Expect = 6e-34
Identities = 74/128 (57%), Positives = 86/128 (67%), Gaps = 5/128 (3%)
Frame = +3
Query: 249 SEVLEILRQDEQFVRCLHAVGSGGTPG-----WPCDPKNTIILHKPAENEIVXXXXXXXX 413
S VL+ L Q + FV+CLH+VG G WPCDP TII H PA+N I
Sbjct: 354 SHVLKALDQGD-FVKCLHSVGKPLKEGEKDVLWPCDPDRTIITHFPADNYIKSFGSGYGG 412
Query: 414 XXXXXKKCFALRLGSVIARREGWLAEHMLIVGITNPQGKKRYIAAAFPSACGKTNLAMMT 593
KKCFALR+ S IA +EGWLAEHMLI+ +TNP+GKK+YIAAAFPSACGKTNLAM+
Sbjct: 413 NSLLGKKCFALRIASNIALKEGWLAEHMLIMALTNPEGKKKYIAAAFPSACGKTNLAMLK 472
Query: 594 PNTGPGTK 617
P T PG K
Sbjct: 473 P-TIPGWK 479
Score = 55.2 bits (127), Expect = 7e-08
Identities = 22/34 (64%), Positives = 31/34 (91%)
Frame = +1
Query: 151 SMGPVGSPLSKIGVEITDSPYVVYSMRVMTRIGA 252
+MGPVGSP++KIG+++TDS YVV MR+MTR+G+
Sbjct: 321 NMGPVGSPIAKIGIQLTDSEYVVCCMRIMTRMGS 354
Score = 36.7 bits (81), Expect = 0.026
Identities = 19/36 (52%), Positives = 24/36 (66%)
Frame = +2
Query: 605 PGYKVXCVGDDIALMKFRXRTAYFRGHLXRKNGFFG 712
PG+KV CVGDDIA M F +T R + ++GFFG
Sbjct: 476 PGWKVDCVGDDIAWMWF-DKTGQLRA-INPESGFFG 509
>SB_37397| Best HMM Match : PEPCK (HMM E-Value=0)
Length = 613
Score = 135 bits (327), Expect = 4e-32
Identities = 73/128 (57%), Positives = 84/128 (65%), Gaps = 5/128 (3%)
Frame = +3
Query: 249 SEVLEILRQDEQFVRCLHAVGSGGTPG-----WPCDPKNTIILHKPAENEIVXXXXXXXX 413
S VL+ L D FVR +H+VG G WPCDP+ T+I H PA EI
Sbjct: 170 SHVLKALG-DGDFVRGVHSVGFPLKEGVKEVPWPCDPERTLITHFPATREIKSYGSGYGG 228
Query: 414 XXXXXKKCFALRLGSVIARREGWLAEHMLIVGITNPQGKKRYIAAAFPSACGKTNLAMMT 593
KKCFALR+ S IA EGWLAEHM+I+G+TNP+GKK YIAAAFPSACGKTNLAM+T
Sbjct: 229 NSLLGKKCFALRIASNIAHDEGWLAEHMMIMGLTNPEGKKIYIAAAFPSACGKTNLAMLT 288
Query: 594 PNTGPGTK 617
P T PG K
Sbjct: 289 P-TIPGWK 295
Score = 76.6 bits (180), Expect = 3e-14
Identities = 32/43 (74%), Positives = 40/43 (93%)
Frame = +1
Query: 124 GRTMYVIPFSMGPVGSPLSKIGVEITDSPYVVYSMRVMTRIGA 252
GRTMYV+PFSMGPVGSP+SKIG+++TDS YVV MR+MTR+G+
Sbjct: 128 GRTMYVLPFSMGPVGSPISKIGIQLTDSEYVVCCMRIMTRMGS 170
Score = 32.3 bits (70), Expect = 0.55
Identities = 17/38 (44%), Positives = 22/38 (57%)
Frame = +2
Query: 605 PGYKVXCVGDDIALMKFRXRTAYFRGHLXRKNGFFGSC 718
PG+K CVGDDI M F + R + ++GFFG C
Sbjct: 292 PGWKCECVGDDINWMWF-DKDGTLRA-INPESGFFGVC 327
>SB_50513| Best HMM Match : Lig_chan (HMM E-Value=0.0083)
Length = 417
Score = 28.7 bits (61), Expect = 6.8
Identities = 14/44 (31%), Positives = 26/44 (59%)
Frame = +2
Query: 386 RKLRQWIRRQ*FVGQEVLRSTSGISDRSSRRMAGRTYAYRRHNQ 517
R L +WI+R+ + V + + +SD SSRR +T + R+++
Sbjct: 226 RDLNKWIKREYHYTKTVDKVVTQLSDASSRRRESKTRSPDRYSR 269
>SB_15741| Best HMM Match : MANEC (HMM E-Value=2.7)
Length = 263
Score = 28.3 bits (60), Expect = 9.0
Identities = 20/72 (27%), Positives = 33/72 (45%), Gaps = 2/72 (2%)
Frame = -2
Query: 342 GRRANPECHRSRLREDNERTARLDVEFLELRSNSSHDSHRIN-HVRRIRDFY-ADLRERR 169
GRRA+PE + L + R E +++S + R+N R R +++ +
Sbjct: 127 GRRADPESNTDNLTKTKRRKRLFGGNQTEPVTDASLNEKRLNERFREHRSVIDSNVEDSA 186
Query: 168 SHRTHREWYHVH 133
S R H E + VH
Sbjct: 187 STRRHHERFQVH 198
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 26,371,056
Number of Sequences: 59808
Number of extensions: 540087
Number of successful extensions: 1555
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 1465
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1552
length of database: 16,821,457
effective HSP length: 82
effective length of database: 11,917,201
effective search space used: 2597949818
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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