BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30156.Seq (961 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC576.08c |rps2||40S ribosomal protein S2|Schizosaccharomyces ... 144 1e-35 SPBC1A4.07c |||U3 snoRNP-associated protein Sof1|Schizosaccharom... 28 1.7 SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 28 2.2 SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyc... 26 6.9 >SPCC576.08c |rps2||40S ribosomal protein S2|Schizosaccharomyces pombe|chr 3|||Manual Length = 253 Score = 144 bits (350), Expect = 1e-35 Identities = 67/110 (60%), Positives = 87/110 (79%) Frame = +1 Query: 220 KNRQTREHLLVFLPIKEFEIIDFFLGPSLNDEVLKIMPVQKQTRAGQRTRFKAFVAIGDN 399 K + E L LPIKE++I+D+FL P LNDEV+K++PVQKQTRAGQRTRFKAFV IGD+ Sbjct: 48 KIKSIEEIYLYSLPIKEYQIVDYFL-PRLNDEVMKVVPVQKQTRAGQRTRFKAFVVIGDS 106 Query: 400 NGHIGLGVKCSKEVATAIRGAIILAKLSVLTSPKKLRGNKIGKPHNRPLQ 549 +GH+GLG+KC+KEVATAIRGAII+ KLS++ + G +G PH P++ Sbjct: 107 DGHVGLGIKCAKEVATAIRGAIIMGKLSIMPIRRGYWGTALGDPHTVPVK 156 Score = 51.2 bits (117), Expect = 2e-07 Identities = 20/31 (64%), Positives = 27/31 (87%) Frame = +2 Query: 161 KEDQKEWVPVTKLGRLVREGKIDKLESIYLF 253 ++++KEWVPVTKLGRLV+ GKI +E IYL+ Sbjct: 28 RDEEKEWVPVTKLGRLVKAGKIKSIEEIYLY 58 >SPBC1A4.07c |||U3 snoRNP-associated protein Sof1|Schizosaccharomyces pombe|chr 2|||Manual Length = 436 Score = 28.3 bits (60), Expect = 1.7 Identities = 12/37 (32%), Positives = 22/37 (59%) Frame = +2 Query: 269 NSRSLISSSARP*MMRFLRSCLYRNKHVPDSAHVSRH 379 +SR+ I S+ +++L S R KH+P+ ++RH Sbjct: 348 SSRASIRSTREENRLKYLDSLRERYKHIPEIRRIARH 384 >SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 1420 Score = 27.9 bits (59), Expect = 2.2 Identities = 13/42 (30%), Positives = 21/42 (50%) Frame = -3 Query: 158 RAHDHGRDHDRVHEDRHGLYLHRVIRIRRENRHVHRLEQRPP 33 ++HDHG H + H DR + R R++R ++ PP Sbjct: 720 QSHDHGHSHSKSH-DREKEKEKKKDREHRKHRETEEEDEGPP 760 >SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyces pombe|chr 1|||Manual Length = 503 Score = 26.2 bits (55), Expect = 6.9 Identities = 15/45 (33%), Positives = 25/45 (55%), Gaps = 2/45 (4%) Frame = -3 Query: 503 FFGLVRTDSLARIIAPRMAVATS--LLHFTPKPI*PLLSPMATNA 375 F L ++ RII PR+A++ S L + T K + +L+ M + A Sbjct: 238 FLNLANDPTIERIITPRLALSASEFLAYQTEKHVLTILTDMTSYA 282 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,374,238 Number of Sequences: 5004 Number of extensions: 65092 Number of successful extensions: 150 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 143 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 149 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 491307756 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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