BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30132.Seq (689 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac... 30 0.27 SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 29 0.84 SPBC19G7.07c |||conserved fungal protein|Schizosaccharomyces pom... 28 1.1 SPAC343.14c |||translation initiation factor eIF2B|Schizosacchar... 27 1.9 SPAC4H3.11c |ppc89|mug127|spindle pole body protein Ppc89|Schizo... 27 3.4 SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 26 4.5 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 26 4.5 SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr ... 26 4.5 SPAC14C4.06c |||poly|Schizosaccharomyces pombe|chr 1|||Manual 26 5.9 SPBC1683.08 |ght4||hexose transporter Ght4 |Schizosaccharomyces ... 25 7.8 >SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 882 Score = 30.3 bits (65), Expect = 0.27 Identities = 26/80 (32%), Positives = 39/80 (48%), Gaps = 2/80 (2%) Frame = +2 Query: 293 STQTGPSGL-VESLEMVRRGIILEQAANQRPLKGILNAASALDAFK*STRITKLKLTSRP 469 S+ T PS + S +V R A KG+ NAAS+ +F S I+K TSR Sbjct: 589 SSPTKPSTTSLRSQSLVNRFASSTLKAPSSSSKGVSNAASSKQSFPSSPSISKKLETSRL 648 Query: 470 SIVTVP-SAIFATRSIVQHP 526 S +P S + A ++ ++P Sbjct: 649 STKKLPGSTMKAASALKEYP 668 >SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosaccharomyces pombe|chr 2|||Manual Length = 933 Score = 28.7 bits (61), Expect = 0.84 Identities = 10/39 (25%), Positives = 20/39 (51%) Frame = +1 Query: 409 CSRCLQMINENNKIEIDKQAFHSHCAKCYFCHTVHRPTS 525 C +C + + +++ + +HS C KC C+ P+S Sbjct: 24 CIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSS 62 >SPBC19G7.07c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 687 Score = 28.3 bits (60), Expect = 1.1 Identities = 12/19 (63%), Positives = 13/19 (68%) Frame = +3 Query: 546 FCEPCFQHHVLHRHKDNPN 602 FC PC + LH HKDNPN Sbjct: 17 FCGPCRR---LHYHKDNPN 32 >SPAC343.14c |||translation initiation factor eIF2B|Schizosaccharomyces pombe|chr 1|||Manual Length = 393 Score = 27.5 bits (58), Expect = 1.9 Identities = 12/22 (54%), Positives = 14/22 (63%) Frame = +2 Query: 32 HTYPKKSSLIMDKGQEQVKKPF 97 HTYP SSLI D +V+ PF Sbjct: 8 HTYPAVSSLIADLKSRKVQGPF 29 >SPAC4H3.11c |ppc89|mug127|spindle pole body protein Ppc89|Schizosaccharomyces pombe|chr 1|||Manual Length = 783 Score = 26.6 bits (56), Expect = 3.4 Identities = 12/40 (30%), Positives = 24/40 (60%) Frame = +1 Query: 349 DNPGASSKSEASEGNTKRSVCSRCLQMINENNKIEIDKQA 468 D+P +S E+ EG+ + S+ S +++++ E +KQA Sbjct: 557 DSPNVASGVESDEGSEEISLDSEYSDILSDDGDFEKEKQA 596 >SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1133 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/26 (42%), Positives = 15/26 (57%) Frame = -1 Query: 317 VHWAQFVWTDGHSPLNRSSTCA*SIH 240 VHW + V +GH+ NR S A + H Sbjct: 648 VHWFRVVLDEGHNIRNRESKTAKACH 673 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 26.2 bits (55), Expect = 4.5 Identities = 15/45 (33%), Positives = 20/45 (44%) Frame = +1 Query: 517 PTSTSAMVKSSASLASNITSCTDIRTTPMXXXXXXXXXXRTPDLP 651 PTSTS+ +S L + TSCT + P TP +P Sbjct: 152 PTSTSSTDTNSNPLPTTSTSCTTSTSIPPTGGSSSLSTPITPTVP 196 Score = 26.2 bits (55), Expect = 4.5 Identities = 13/28 (46%), Positives = 16/28 (57%) Frame = +1 Query: 517 PTSTSAMVKSSASLASNITSCTDIRTTP 600 P STS+ SS+ L S TSCT + P Sbjct: 319 PASTSSTGTSSSPLPSTSTSCTTSTSIP 346 Score = 26.2 bits (55), Expect = 4.5 Identities = 13/28 (46%), Positives = 16/28 (57%) Frame = +1 Query: 517 PTSTSAMVKSSASLASNITSCTDIRTTP 600 P STS+ SS+ L S TSCT + P Sbjct: 373 PASTSSTGTSSSPLLSTSTSCTTSTSIP 400 Score = 25.8 bits (54), Expect = 5.9 Identities = 12/28 (42%), Positives = 17/28 (60%) Frame = +1 Query: 517 PTSTSAMVKSSASLASNITSCTDIRTTP 600 PTSTS+ +S+ L + TSCT + P Sbjct: 209 PTSTSSTDTNSSPLPTTSTSCTTSTSIP 236 Score = 25.8 bits (54), Expect = 5.9 Identities = 12/28 (42%), Positives = 17/28 (60%) Frame = +1 Query: 517 PTSTSAMVKSSASLASNITSCTDIRTTP 600 PTSTS+ +S+ L + TSCT + P Sbjct: 265 PTSTSSTDTNSSPLPTTSTSCTTSTSIP 292 >SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1337 Score = 26.2 bits (55), Expect = 4.5 Identities = 12/29 (41%), Positives = 17/29 (58%) Frame = -1 Query: 281 SPLNRSSTCA*SIHCRIRARRTSSPPTEL 195 S N SST + S H +I + R S PP ++ Sbjct: 26 SSSNNSSTASGSRHVKIESNRVSLPPLKI 54 >SPAC14C4.06c |||poly|Schizosaccharomyces pombe|chr 1|||Manual Length = 307 Score = 25.8 bits (54), Expect = 5.9 Identities = 16/62 (25%), Positives = 24/62 (38%) Frame = +1 Query: 379 ASEGNTKRSVCSRCLQMINENNKIEIDKQAFHSHCAKCYFCHTVHRPTSTSAMVKSSASL 558 A+ N +SV + + + ++ + K A A C F H MV SS Sbjct: 158 AASFNYSKSVMPAAKRALTQTQEVPLCKYADKCSRANCIFAHPTPAAAPGEGMVLSSEMC 217 Query: 559 AS 564 AS Sbjct: 218 AS 219 >SPBC1683.08 |ght4||hexose transporter Ght4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 557 Score = 25.4 bits (53), Expect = 7.8 Identities = 14/37 (37%), Positives = 21/37 (56%) Frame = +1 Query: 334 NGQEGDNPGASSKSEASEGNTKRSVCSRCLQMINENN 444 +G + D+ ASS+S AS G RSV R + E++ Sbjct: 517 DGADNDDSSASSESFASAGAHSRSVFPRRSNVSEESH 553 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,934,997 Number of Sequences: 5004 Number of extensions: 61162 Number of successful extensions: 182 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 171 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 182 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 319939482 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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