BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30123.Seq (694 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 139 3e-34 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 86 5e-18 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 86 5e-18 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 69 9e-13 SPAC343.17c |||WD repeat protein, human WDR70 family|Schizosacch... 27 1.9 SPBC947.14c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 27 3.4 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 4.5 SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|ch... 26 5.9 SPCC736.11 |ago1|csp9|argonaute|Schizosaccharomyces pombe|chr 3|... 25 7.9 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 139 bits (337), Expect = 3e-34 Identities = 71/145 (48%), Positives = 90/145 (62%) Frame = +3 Query: 255 QLERINVYYNEASAGKYVPRTVLIDLKPATMDAVRSGPFGCLFRPDNFVYGQNCAANNWA 434 Q ER+NVY+NEA+ GKYVPR VL+DL+P TMDAV+SG FG LFRPDN +YGQ+ A N WA Sbjct: 43 QHERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWA 102 Query: 435 KGHYTEGVEILESALDVIRREAEGWIACKFSDVTLARWRYRFRIRYATS*TGYAKSIPTE 614 KGHYTEG E+ ++ LDV+RREAE A + +T + + + P Sbjct: 103 KGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDR 162 Query: 615 LY*PFQSFQVPECRICVVEPYNTTL 689 + F P+ VVEPYN TL Sbjct: 163 MMATFSVAPAPKSSDTVVEPYNATL 187 Score = 66.9 bits (156), Expect = 3e-12 Identities = 24/41 (58%), Positives = 32/41 (78%) Frame = +1 Query: 130 MREIINLQVGSCGNQIGGKFWEVISDEHGIDPSGCYHGDSD 252 MREI+++Q G CGNQ+G FW I+DEHG+D +G YHG S+ Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSE 41 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 85.8 bits (203), Expect = 5e-18 Identities = 35/76 (46%), Positives = 56/76 (73%) Frame = +3 Query: 276 YYNEASAGKYVPRTVLIDLKPATMDAVRSGPFGCLFRPDNFVYGQNCAANNWAKGHYTEG 455 +++E GK+VPR++ +DL+P +D VR+GP+ LF P+ V G+ A+NN+A+GHYT G Sbjct: 56 FFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVG 115 Query: 456 VEILESALDVIRREAE 503 E+++S L+ IRR A+ Sbjct: 116 KEMIDSVLERIRRMAD 131 Score = 44.0 bits (99), Expect = 2e-05 Identities = 20/47 (42%), Positives = 28/47 (59%) Frame = +1 Query: 130 MREIINLQVGSCGNQIGGKFWEVISDEHGIDPSGCYHGDSDLNSKGS 270 MRE+I++ VG G QIG WE+ EHGI P G +S+++ S Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNS 47 Score = 26.6 bits (56), Expect = 3.4 Identities = 11/25 (44%), Positives = 17/25 (68%) Frame = +2 Query: 581 VNRIREEYPDRIILTFSVFPSPRVS 655 + R+ EY + L FSV+P+P+VS Sbjct: 158 LERLNMEYGKKSNLQFSVYPAPQVS 182 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 85.8 bits (203), Expect = 5e-18 Identities = 50/147 (34%), Positives = 79/147 (53%), Gaps = 7/147 (4%) Frame = +3 Query: 270 NVYYNEASAGKYVPRTVLIDLKPATMDAVRSGPFGCLFRPDNFVYGQNCAANNWAKGHYT 449 + +++E GKYVPR++ +DL+P +D VR+GP+ LF P+ + G+ A+NN+A+GHYT Sbjct: 50 STFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYT 109 Query: 450 EGVEILESALDVIRREA------EGWIAC-KFSDVTLARWRYRFRIRYATS*TGYAKSIP 608 G E+++ D IRR A +G++ F T + + R A Y K Sbjct: 110 VGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAME---YTKKSK 166 Query: 609 TELY*PFQSFQVPECRICVVEPYNTTL 689 + F + P+ VVEPYN+ L Sbjct: 167 LQ----FSVYPAPQVSTSVVEPYNSVL 189 Score = 46.4 bits (105), Expect = 4e-06 Identities = 23/51 (45%), Positives = 30/51 (58%), Gaps = 1/51 (1%) Frame = +1 Query: 130 MREIINLQVGSCGNQIGGKFWEVISDEHGIDPSGCYHGD-SDLNSKGSTCT 279 MREII++ VG G QIG WE+ EHGI P+G + + + NS G T Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFST 51 Score = 26.6 bits (56), Expect = 3.4 Identities = 11/25 (44%), Positives = 17/25 (68%) Frame = +2 Query: 581 VNRIREEYPDRIILTFSVFPSPRVS 655 + R+ EY + L FSV+P+P+VS Sbjct: 154 LERLAMEYTKKSKLQFSVYPAPQVS 178 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 68.5 bits (160), Expect = 9e-13 Identities = 31/85 (36%), Positives = 54/85 (63%), Gaps = 2/85 (2%) Frame = +3 Query: 258 LERINVYYNEASAGKYVPRTVLIDLKPATMDAVRSGPFGCLFRPDNFVYGQN--CAANNW 431 ++R +V++ ++ +Y+PR +LIDL+P ++ + S +G L+ P+N + +N A NNW Sbjct: 45 VDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNW 104 Query: 432 AKGHYTEGVEILESALDVIRREAEG 506 A G Y+ I E +D+I REA+G Sbjct: 105 ANG-YSHAERIFEDIMDMIDREADG 128 Score = 52.0 bits (119), Expect = 8e-08 Identities = 21/33 (63%), Positives = 24/33 (72%) Frame = +1 Query: 133 REIINLQVGSCGNQIGGKFWEVISDEHGIDPSG 231 REII LQ G CGNQIG +FW+ + EHGI P G Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDG 35 Score = 31.9 bits (69), Expect = 0.090 Identities = 14/30 (46%), Positives = 21/30 (70%), Gaps = 1/30 (3%) Frame = +2 Query: 575 YFVNRIREEYPDRIILTFSVFP-SPRVSDL 661 + + R+ + YP +II T+SVFP S VSD+ Sbjct: 152 FLLERLNDRYPKKIIQTYSVFPNSQSVSDV 181 >SPAC343.17c |||WD repeat protein, human WDR70 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 576 Score = 27.5 bits (58), Expect = 1.9 Identities = 17/50 (34%), Positives = 21/50 (42%) Frame = +2 Query: 224 QADATTATPTSTRKDQRVLQRGIGWEICSSHGSYRLEAGHDGRGEVGTLR 373 Q + T P KD + Q+GI S G+Y L G D V LR Sbjct: 279 QKGSRTKRPVMKIKDAHLPQQGISCLSFSQDGNYLLSRGEDNALRVWDLR 328 >SPBC947.14c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 103 Score = 26.6 bits (56), Expect = 3.4 Identities = 14/42 (33%), Positives = 23/42 (54%) Frame = +3 Query: 213 RHRSKRMLPRRLRPQLERINVYYNEASAGKYVPRTVLIDLKP 338 R R ++M P++ QL +N + KY+P+ V+ LKP Sbjct: 34 RARLQKMPPQQANKQLNALNNLLDNVYWNKYIPKQVV--LKP 73 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 26.2 bits (55), Expect = 4.5 Identities = 10/28 (35%), Positives = 18/28 (64%) Frame = +3 Query: 432 AKGHYTEGVEILESALDVIRREAEGWIA 515 A+GH GVE++ + D +R+++E A Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTA 210 >SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|chr 3|||Manual Length = 872 Score = 25.8 bits (54), Expect = 5.9 Identities = 9/27 (33%), Positives = 16/27 (59%) Frame = +1 Query: 361 RDPSDAYSDRTISSTDKTVRPTTGRRD 441 + P + +SD+TI S T+ PT ++ Sbjct: 702 KTPMNLFSDQTIGSITNTIEPTAAAKN 728 >SPCC736.11 |ago1|csp9|argonaute|Schizosaccharomyces pombe|chr 3|||Manual Length = 834 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/44 (29%), Positives = 18/44 (40%) Frame = +1 Query: 181 GKFWEVISDEHGIDPSGCYHGDSDLNSKGSTCTTTRHRLGNMFL 312 G+F VI+DE C+ N K CTT + F+ Sbjct: 656 GQFLSVINDELSQIKEACHSLSPKYNPKILVCTTQKRHHARFFI 699 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,796,410 Number of Sequences: 5004 Number of extensions: 56246 Number of successful extensions: 163 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 161 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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