Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30115.Seq
(690 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g03340.1 68418.m00286 cell division cycle protein 48, putativ... 116 1e-26
At3g09840.1 68416.m01174 cell division cycle protein 48 (CDC48A)... 116 1e-26
At3g53230.1 68416.m05865 cell division cycle protein 48, putativ... 111 4e-25
At4g13885.1 68417.m02151 3'-5' exonuclease-related contains weak... 31 0.95
At3g26630.1 68416.m03328 pentatricopeptide (PPR) repeat-containi... 30 1.3
At1g30750.1 68414.m03758 expressed protein 30 1.3
At3g10160.1 68416.m01218 dihydrofolate synthetase/folylpolygluta... 29 2.9
At2g46430.1 68415.m05778 cyclic nucleotide-regulated ion channel... 29 2.9
At1g31060.1 68414.m03802 expressed protein similar to EST gb|T43267 29 2.9
At5g50220.1 68418.m06220 F-box family protein contains F-box dom... 28 5.1
At5g05980.1 68418.m00662 dihydrofolate synthetase/folylpolygluta... 28 5.1
At2g46440.1 68415.m05779 cyclic nucleotide-regulated ion channel... 28 5.1
At2g14120.2 68415.m01573 dynamin-like protein 2b (ADL2b) identic... 28 6.7
At2g14120.1 68415.m01572 dynamin-like protein 2b (ADL2b) identic... 28 6.7
At5g10520.1 68418.m01218 protein kinase family protein contains ... 27 8.9
At1g49400.1 68414.m05537 ribosomal protein S17 family protein si... 27 8.9
>At5g03340.1 68418.m00286 cell division cycle protein 48, putative /
CDC48, putative very strong similarity to SP|P54609 Cell
division cycle protein 48 homolog {Arabidopsis
thaliana}; contains Pfam profiles PF00004: ATPase AAA
family, PF02359: Cell division protein 48 (CDC48)
N-terminal domain; supporting cDNA
gi|26449351|dbj|AK117125.1|
Length = 810
Score = 116 bits (280), Expect = 1e-26
Identities = 49/85 (57%), Positives = 62/85 (72%)
Frame = +2
Query: 257 LSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHILPIDDSVEGLTGNLF 436
L+D+ C + KIRM DV+S+ CP VKYGKRVHILP+DD+VEG+TGNLF
Sbjct: 76 LADETCEEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGKRVHILPVDDTVEGVTGNLF 135
Query: 437 EVYLKPYFMEAYRPIHRDDTFMVRG 511
+ YLKPYF+EAYRP+ + D F+VRG
Sbjct: 136 DAYLKPYFLEAYRPVRKGDLFLVRG 160
Score = 99.1 bits (236), Expect = 2e-21
Identities = 44/67 (65%), Positives = 57/67 (85%)
Frame = +3
Query: 54 ADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKR 233
+D+K+ D STAIL RK PNRL+V+EA++DDNSVV+L ME+LQLFRGDT+L+KGK+
Sbjct: 8 SDSKTKKDFSTAILERKKSPNRLVVDEAINDDNSVVSLHPTTMEKLQLFRGDTILIKGKK 67
Query: 234 RKETVCI 254
RK+TVCI
Sbjct: 68 RKDTVCI 74
Score = 93.1 bits (221), Expect = 2e-19
Identities = 39/57 (68%), Positives = 50/57 (87%)
Frame = +1
Query: 511 GMRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIKREEEEEALNAVGYDDIGGCRKQL 681
GMR+VEFKV+ETDP+ +C+VAPDT I C+GEP+KRE+EE L+ VGYDD+GG RKQ+
Sbjct: 161 GMRSVEFKVIETDPAEYCVVAPDTEIFCEGEPVKREDEER-LDEVGYDDVGGVRKQM 216
>At3g09840.1 68416.m01174 cell division cycle protein 48 (CDC48A)
(CDC48) identical to SP|P54609 Cell division cycle
protein 48 homolog {Arabidopsis thaliana}
Length = 809
Score = 116 bits (280), Expect = 1e-26
Identities = 49/85 (57%), Positives = 62/85 (72%)
Frame = +2
Query: 257 LSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHILPIDDSVEGLTGNLF 436
L+D+ C + KIRM DV+S+ CP VKYGKRVHILP+DD+VEG+TGNLF
Sbjct: 76 LADETCEEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGKRVHILPVDDTVEGVTGNLF 135
Query: 437 EVYLKPYFMEAYRPIHRDDTFMVRG 511
+ YLKPYF+EAYRP+ + D F+VRG
Sbjct: 136 DAYLKPYFLEAYRPVRKGDLFLVRG 160
Score = 101 bits (243), Expect = 3e-22
Identities = 46/67 (68%), Positives = 58/67 (86%)
Frame = +3
Query: 54 ADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKR 233
+D+KS D STAIL RK PNRL+V+EA++DDNSVV+L A ME+LQLFRGDT+L+KGK+
Sbjct: 8 SDSKSKKDFSTAILERKKSPNRLVVDEAINDDNSVVSLHPATMEKLQLFRGDTILIKGKK 67
Query: 234 RKETVCI 254
RK+TVCI
Sbjct: 68 RKDTVCI 74
Score = 92.7 bits (220), Expect = 2e-19
Identities = 39/57 (68%), Positives = 50/57 (87%)
Frame = +1
Query: 511 GMRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIKREEEEEALNAVGYDDIGGCRKQL 681
GMR+VEFKV+ETDP+ +C+VAPDT I C+GEP+KRE+EE L+ VGYDD+GG RKQ+
Sbjct: 161 GMRSVEFKVIETDPAEYCVVAPDTEIFCEGEPVKREDEER-LDDVGYDDVGGVRKQM 216
>At3g53230.1 68416.m05865 cell division cycle protein 48, putative /
CDC48, putative very strong similarity to SP|P54609 Cell
division cycle protein 48 homolog {Arabidopsis
thaliana}; contains Pfam profiles PF00004: ATPase AAA
family, PF02359: Cell division protein 48 (CDC48)
N-terminal domain
Length = 815
Score = 111 bits (267), Expect = 4e-25
Identities = 45/85 (52%), Positives = 61/85 (71%)
Frame = +2
Query: 257 LSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHILPIDDSVEGLTGNLF 436
L+D+ C + KIRM DV+S+ CP VKYG RVHILP+DD++EG++GN+F
Sbjct: 77 LADETCDEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGNRVHILPLDDTIEGVSGNIF 136
Query: 437 EVYLKPYFMEAYRPIHRDDTFMVRG 511
+ YLKPYF+EAYRP+ + D F+VRG
Sbjct: 137 DAYLKPYFLEAYRPVRKGDLFLVRG 161
Score = 93.1 bits (221), Expect = 2e-19
Identities = 39/57 (68%), Positives = 50/57 (87%)
Frame = +1
Query: 511 GMRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIKREEEEEALNAVGYDDIGGCRKQL 681
GMR++EFKV+ETDP+ +C+VAPDT I C+GEPIKRE+EE L+ VGYDD+GG RKQ+
Sbjct: 162 GMRSIEFKVIETDPAEYCVVAPDTEIFCEGEPIKREDEER-LDEVGYDDVGGVRKQM 217
Score = 89.4 bits (212), Expect = 2e-18
Identities = 42/68 (61%), Positives = 56/68 (82%), Gaps = 1/68 (1%)
Frame = +3
Query: 54 ADNK-SPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGK 230
+D+K + D STAIL +K NRL+V+EA++DDNSVV+L ME+LQLFRGDT+L+KGK
Sbjct: 8 SDSKGTKKDFSTAILEKKKAANRLVVDEAINDDNSVVSLHPDTMEKLQLFRGDTILIKGK 67
Query: 231 RRKETVCI 254
+RK+TVCI
Sbjct: 68 KRKDTVCI 75
>At4g13885.1 68417.m02151 3'-5' exonuclease-related contains weak
similarity to Pfam domain PF01612: 3'-5' exonuclease
Length = 263
Score = 30.7 bits (66), Expect = 0.95
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = -2
Query: 206 TTEELKLLHFGL*KCHD*VVIAD 138
TTEELK+ H+ L KC D +V+A+
Sbjct: 3 TTEELKISHYKLYKCFDFLVVAN 25
>At3g26630.1 68416.m03328 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile: PF01535 PPR repeat
Length = 455
Score = 30.3 bits (65), Expect = 1.3
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = -3
Query: 361 RTRSDGYHIRKTHTKVVSHNTVHPNFLIRAII 266
RT S+ +++ HTK++ HN + L+R +I
Sbjct: 28 RTCSNFSQLKQIHTKIIKHNLTNDQLLVRQLI 59
>At1g30750.1 68414.m03758 expressed protein
Length = 212
Score = 30.3 bits (65), Expect = 1.3
Identities = 14/59 (23%), Positives = 28/59 (47%)
Frame = +1
Query: 91 SSVARTDPTVSLSKKQSAMTTQSWHFHRPKWSNFNSSVVTQSCSRANAARKPFASALRR 267
SS +P + S++ F++P + FN + + S S ++A +PF + L +
Sbjct: 115 SSTGLNNPILGQPSTSSSLNQPLTGFNQPSSTGFNQPISSSSSSSSSAQNQPFTNRLNQ 173
>At3g10160.1 68416.m01218 dihydrofolate
synthetase/folylpolyglutamate synthetase (DHFS/FPGS3)
nearly identical to gi:17976757
Length = 464
Score = 29.1 bits (62), Expect = 2.9
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = -1
Query: 534 FELDGAHAPRTMKVSSRW 481
F LDGAH+P +M+ RW
Sbjct: 250 FYLDGAHSPESMEACGRW 267
>At2g46430.1 68415.m05778 cyclic nucleotide-regulated ion channel /
cyclic nucleotide-gated channel (CNGC3) identical to
cyclic nucleotide and calmodulin-regulated ion channel
GI:4581201 from [Arabidopsis thaliana]
Length = 706
Score = 29.1 bits (62), Expect = 2.9
Identities = 21/68 (30%), Positives = 28/68 (41%), Gaps = 2/68 (2%)
Frame = +1
Query: 490 RHLHGPRGMRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIK--REEEEEALNAVGYDDIG 663
R LH + F V+ C + HC + K REEE + N + DD G
Sbjct: 592 RRLHSKQLRHMFRFYSVQWQTWAACFIQAAWKRHCRRKLSKALREEEGKLHNTLQNDDSG 651
Query: 664 GCRKQLGA 687
G + LGA
Sbjct: 652 GNKLNLGA 659
>At1g31060.1 68414.m03802 expressed protein similar to EST gb|T43267
Length = 106
Score = 29.1 bits (62), Expect = 2.9
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = +2
Query: 500 MVRGACAPSSSKWSKQIHHH 559
M+R C PSSS W HHH
Sbjct: 1 MLREECTPSSSWWEDVQHHH 20
>At5g50220.1 68418.m06220 F-box family protein contains F-box domain
Pfam:PF00646
Length = 357
Score = 28.3 bits (60), Expect = 5.1
Identities = 18/78 (23%), Positives = 34/78 (43%), Gaps = 2/78 (2%)
Frame = -1
Query: 594 TVYYGIRSHDAKW*WICFDHF--ELDGAHAPRTMKVSSRWIGR*ASMKYGFKYTSNRLPV 421
T+YY + + + + +CFD + D AP+TM + R+ K GF NR+ +
Sbjct: 206 TIYYQAKQYGSTYVLLCFDVISEKFDQVEAPKTM-MDHRYTLINYQGKLGFMCCQNRVEI 264
Query: 420 RPSTESSIGSMWTRFPYF 367
W++ ++
Sbjct: 265 WVMKNDEKKQEWSKIFFY 282
>At5g05980.1 68418.m00662 dihydrofolate
synthetase/folylpolyglutamate synthetase (DHFS/FPGS2)
nearly identical to gi:17976705; identical to cDNA
dihydrofolate synthetase/folylpolyglutamate synthetase
(dhfs/fpgs2 gene) GI:17976704
Length = 571
Score = 28.3 bits (60), Expect = 5.1
Identities = 9/18 (50%), Positives = 14/18 (77%)
Frame = -1
Query: 534 FELDGAHAPRTMKVSSRW 481
F LDGAH+P +M+ ++W
Sbjct: 382 FYLDGAHSPESMEACAKW 399
>At2g46440.1 68415.m05779 cyclic nucleotide-regulated ion channel,
putative (CNGC11) similar to cyclic nucleotide and
calmodulin-regulated ion channel (cngc3) GI:4581201 from
[Arabidopsis thaliana]
Length = 588
Score = 28.3 bits (60), Expect = 5.1
Identities = 20/68 (29%), Positives = 28/68 (41%), Gaps = 2/68 (2%)
Frame = +1
Query: 490 RHLHGPRGMRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIK--REEEEEALNAVGYDDIG 663
R LH + F ++ C + HC + K REEE + N + DD G
Sbjct: 517 RRLHSKQLQHMFRFYSLQWQTWAACFIQAAWKRHCRRKLSKALREEEGKLHNTLQNDDSG 576
Query: 664 GCRKQLGA 687
G + LGA
Sbjct: 577 GNKLNLGA 584
>At2g14120.2 68415.m01573 dynamin-like protein 2b (ADL2b) identical
to dynamin like protein 2b (ADL2b) [Arabidopsis
thaliana] GI:19032339
Length = 780
Score = 27.9 bits (59), Expect = 6.7
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = -3
Query: 304 NTVHPNFLIRAIIV*EQMQTVSLRRLP 224
NT HPNF+ V + MQTV R+P
Sbjct: 508 NTSHPNFIGGTKAVEQAMQTVKSSRIP 534
>At2g14120.1 68415.m01572 dynamin-like protein 2b (ADL2b) identical
to dynamin like protein 2b (ADL2b) [Arabidopsis
thaliana] GI:19032339
Length = 780
Score = 27.9 bits (59), Expect = 6.7
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = -3
Query: 304 NTVHPNFLIRAIIV*EQMQTVSLRRLP 224
NT HPNF+ V + MQTV R+P
Sbjct: 508 NTSHPNFIGGTKAVEQAMQTVKSSRIP 534
>At5g10520.1 68418.m01218 protein kinase family protein contains
protein kinase domain, INTERPRO:IPR000719
Length = 467
Score = 27.5 bits (58), Expect = 8.9
Identities = 14/49 (28%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
Frame = +1
Query: 49 KWQIIKALMIYR-PRSSVARTDPTVSLSKKQSAMTTQSWHFHRPKWSNF 192
++ +I L Y R ++ R P L+ +SA T +++ +P W NF
Sbjct: 95 RFSVIPLLASYELTRKNLRRKQP--KLTPSESAFTCEAFFMAKPSWRNF 141
>At1g49400.1 68414.m05537 ribosomal protein S17 family protein
similar to 40S ribosomal protein S17 GI:1620985 from
[Nicotiana plumbaginifolia]
Length = 116
Score = 27.5 bits (58), Expect = 8.9
Identities = 18/44 (40%), Positives = 23/44 (52%)
Frame = +1
Query: 28 SNNKF*IKWQIIKALMIYRPRSSVARTDPTVSLSKKQSAMTTQS 159
S NK I +IIK IY P+++ A + S S SA TT S
Sbjct: 64 SKNKHWIVAEIIKKARIYSPKAAAAAVSASASAS---SASTTDS 104
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,270,404
Number of Sequences: 28952
Number of extensions: 320853
Number of successful extensions: 902
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 877
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 899
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1467502800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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