BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30110.Seq
(444 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g26230.1 68417.m03776 60S ribosomal protein L31 (RPL31B) ribo... 102 1e-22
At5g56710.1 68418.m07078 60S ribosomal protein L31 (RPL31C) 99 7e-22
At2g19740.1 68415.m02306 60S ribosomal protein L31 (RPL31A) 99 1e-21
At4g17250.1 68417.m02594 expressed protein 28 2.5
At3g19400.2 68416.m02460 cysteine proteinase, putative non-conse... 28 2.5
At3g19400.1 68416.m02461 cysteine proteinase, putative non-conse... 28 2.5
At5g07150.1 68418.m00815 leucine-rich repeat family protein cont... 27 4.3
At3g51220.1 68416.m05607 expressed protein contains Pfam doamin ... 27 4.3
At5g40170.1 68418.m04875 disease resistance family protein conta... 27 5.7
At1g32900.1 68414.m04053 starch synthase, putative similar to st... 27 5.7
At3g43960.1 68416.m04706 cysteine proteinase, putative contains ... 26 10.0
>At4g26230.1 68417.m03776 60S ribosomal protein L31 (RPL31B)
ribosomal protein L31, Nicotiana glutinosa, U23784
Length = 119
Score = 102 bits (244), Expect = 1e-22
Identities = 46/66 (69%), Positives = 56/66 (84%)
Frame = +2
Query: 56 ERKGKSAINEVVTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLN 235
E+KG+ EVVTREYT+NLH+RLH FKK+AP+AIKEIRKFAEK+MGT D+RVD +LN
Sbjct: 3 EKKGRK--EEVVTREYTINLHRRLHSCTFKKKAPKAIKEIRKFAEKEMGTKDVRVDVKLN 60
Query: 236 KFLWSK 253
K +WSK
Sbjct: 61 KQIWSK 66
Score = 31.9 bits (69), Expect = 0.20
Identities = 16/50 (32%), Positives = 25/50 (50%), Gaps = 2/50 (4%)
Frame = +1
Query: 253 GVRNVPFXXXXXXXXXXNDDEDSAHKLFTLVTY--VPVASIKGLQTENVD 396
G+R P NDDED+ + F+LVT +P + GL T+ ++
Sbjct: 67 GIRGPPRRIRVRVARKRNDDEDAKEEFFSLVTVAEIPAEGLSGLGTKIIE 116
>At5g56710.1 68418.m07078 60S ribosomal protein L31 (RPL31C)
Length = 119
Score = 99 bits (238), Expect = 7e-22
Identities = 45/66 (68%), Positives = 55/66 (83%)
Frame = +2
Query: 56 ERKGKSAINEVVTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLN 235
E+KG+ EV+TREYT+NLH+RLH FKK+AP+AIKEIRKFAEK MGT D+RVD +LN
Sbjct: 3 EKKGRK--EEVITREYTINLHRRLHKCTFKKKAPKAIKEIRKFAEKAMGTKDVRVDVKLN 60
Query: 236 KFLWSK 253
K +WSK
Sbjct: 61 KQIWSK 66
Score = 31.9 bits (69), Expect = 0.20
Identities = 16/50 (32%), Positives = 25/50 (50%), Gaps = 2/50 (4%)
Frame = +1
Query: 253 GVRNVPFXXXXXXXXXXNDDEDSAHKLFTLVTY--VPVASIKGLQTENVD 396
G+R P NDDED+ + F+LVT +P + GL T+ ++
Sbjct: 67 GIRGPPRRIRVRVARKRNDDEDAKEEFFSLVTVAEIPAEGLSGLGTKVIE 116
>At2g19740.1 68415.m02306 60S ribosomal protein L31 (RPL31A)
Length = 119
Score = 99.1 bits (236), Expect = 1e-21
Identities = 45/64 (70%), Positives = 51/64 (79%)
Frame = +2
Query: 62 KGKSAINEVVTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLNKF 241
KGK EVVTREYT+NLH+RLH FKK+AP AIKEIRKFA K MGT D+RVD +LNK
Sbjct: 3 KGKGRKEEVVTREYTINLHRRLHSCTFKKKAPNAIKEIRKFALKAMGTKDVRVDVKLNKQ 62
Query: 242 LWSK 253
+WSK
Sbjct: 63 IWSK 66
Score = 32.3 bits (70), Expect = 0.15
Identities = 16/53 (30%), Positives = 26/53 (49%), Gaps = 2/53 (3%)
Frame = +1
Query: 253 GVRNVPFXXXXXXXXXXNDDEDSAHKLFTLVTY--VPVASIKGLQTENVDASQ 405
G+R P NDDED+ + F+LVT +P + GL T+ ++ +
Sbjct: 67 GIRGPPRRIRVRVARKRNDDEDAKEEFFSLVTVAEIPAEGLSGLGTKVIEEEE 119
>At4g17250.1 68417.m02594 expressed protein
Length = 416
Score = 28.3 bits (60), Expect = 2.5
Identities = 13/40 (32%), Positives = 22/40 (55%)
Frame = +2
Query: 104 TVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVD 223
TV KR + +KKR A+K+ + E++ G P ++ D
Sbjct: 169 TVPATKRFLELKYKKRYEFALKQCPSYTERRRGVPKLKED 208
>At3g19400.2 68416.m02460 cysteine proteinase, putative
non-consensus AT acceptor site at exon 3; contains
similarity to cysteine protease CYP1 GI:2828252, TDI-65
GI:5726641 from [Lycopersicon esculentum]
Length = 290
Score = 28.3 bits (60), Expect = 2.5
Identities = 13/48 (27%), Positives = 24/48 (50%)
Frame = +2
Query: 98 EYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLNKF 241
++ V K +G+G K+R + K+ KF ++ PD + L +F
Sbjct: 46 QWLVENRKNYNGLGEKERRFKIFKDNLKFVDEHNSVPDRTFEVGLTRF 93
>At3g19400.1 68416.m02461 cysteine proteinase, putative
non-consensus AT acceptor site at exon 3; contains
similarity to cysteine protease CYP1 GI:2828252, TDI-65
GI:5726641 from [Lycopersicon esculentum]
Length = 362
Score = 28.3 bits (60), Expect = 2.5
Identities = 13/48 (27%), Positives = 24/48 (50%)
Frame = +2
Query: 98 EYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLNKF 241
++ V K +G+G K+R + K+ KF ++ PD + L +F
Sbjct: 46 QWLVENRKNYNGLGEKERRFKIFKDNLKFVDEHNSVPDRTFEVGLTRF 93
>At5g07150.1 68418.m00815 leucine-rich repeat family protein
contains weak similarity to LRR receptor-like protein
kinase [Nicotiana tabacum] gi|7672732|gb|AAF66615;
contains Pfam PF00560 domain Leucine Rich Repeat
Length = 553
Score = 27.5 bits (58), Expect = 4.3
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +2
Query: 2 FFFFWYTKLKITMAKPKGERKGKSAINEVVT 94
FFF W K+K+ KP GE + +VVT
Sbjct: 250 FFFLWNQKVKLI--KPWGETGSSGQLQDVVT 278
>At3g51220.1 68416.m05607 expressed protein contains Pfam doamin
PF05701: Plant protein of unknown function (DUF827)
Length = 186
Score = 27.5 bits (58), Expect = 4.3
Identities = 16/71 (22%), Positives = 31/71 (43%)
Frame = +2
Query: 29 KITMAKPKGERKGKSAINEVVTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTP 208
K + K + E K S + E +T+E K H + P+ +++ E + P
Sbjct: 53 KENLKKAEEENKVLSQLIESLTQELETTKEKLNHSLRNFPEHPQVEDDLKFIEESTVNEP 112
Query: 209 DIRVDTRLNKF 241
D + ++N+F
Sbjct: 113 DNITEIKMNRF 123
>At5g40170.1 68418.m04875 disease resistance family protein contains
leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; similar to Cf-4 [Lycopersicon
hirsutum] gi|2808683|emb|CAA05268
Length = 792
Score = 27.1 bits (57), Expect = 5.7
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +2
Query: 215 RVDTRLNKFLWSKESEMFPSVS 280
R+DTR N W+K+S+ F VS
Sbjct: 62 RMDTRANISSWTKDSDSFSGVS 83
>At1g32900.1 68414.m04053 starch synthase, putative similar to
starch synthase SP:Q42857 from [Ipomoea batatas]
Length = 610
Score = 27.1 bits (57), Expect = 5.7
Identities = 14/55 (25%), Positives = 26/55 (47%)
Frame = +2
Query: 56 ERKGKSAINEVVTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRV 220
E+KG + E +++ +N+ + G G KK + ++ KF K +G V
Sbjct: 412 EQKGSDILVEAISKFMGLNVQMVILGTGKKKMEAQILELEEKFPGKAVGVAKFNV 466
>At3g43960.1 68416.m04706 cysteine proteinase, putative contains
similarity to cysteine proteinase RD21A (thiol protease)
GI:435619, SP:P43297 from [Arabidopsis thaliana]
Length = 376
Score = 26.2 bits (55), Expect = 10.0
Identities = 17/55 (30%), Positives = 28/55 (50%), Gaps = 2/55 (3%)
Frame = +2
Query: 83 EVVT--REYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLNKF 241
EV+T ++ V K +G+G K+R + K+ K E+ P+ + LNKF
Sbjct: 36 EVLTMYEQWLVENGKNYNGLGEKERRFKIFKDNLKRIEEHNSDPNRSYERGLNKF 90
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,992,812
Number of Sequences: 28952
Number of extensions: 164393
Number of successful extensions: 363
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 353
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 363
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 712739520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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